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Enzymatic and transport studies in cholesterol fed guinea pigs using intestinal brush border membrane vesicle

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dc.contributor.author Sidhu, G. S.
dc.contributor.author Garg, U C
dc.contributor.author Dhaunsi, G. S.
dc.contributor.author Bhari, S K
dc.contributor.author Ganguly, N K
dc.contributor.author Bhatnagar, Rakesh
dc.date.accessioned 2018-11-15T05:17:02Z
dc.date.available 2018-11-15T05:17:02Z
dc.date.issued 1986
dc.identifier.issn 1421-9867
dc.identifier.issn 0012-2823 (Print)
dc.identifier.uri https://doi.org/10.1159/000199338
dc.identifier.uri http://dl.bhu.ac.in:8080/xmlui/handle/123456789/285
dc.description Full text is available from publishers website. en_US
dc.description.abstract The intestinal absorptive and digestive functions using the brush border membrane (BBM) vesicles were evaluated in guinea pigs receiving cholesterol-supplemented diet for 12 weeks. The Na+-gradient-dependent transport of D-glucose (p < 0.001), L-alanine and L-phenylalanine (p < 0.01) was decreased significantly the BBM of cholesterol-fed animals. The maximal velocity (Vmax) value of the sucrase and leucine aminopeptidase was decreased without any change in the affinity constant (Km) value, demonstrating that the enzyme contents were reduced in response to cholesterol-rich diet. However, both the Km and Vmax values of the alkaline phosphatase decreased markedly, suggesting that a new enzyme of increased substrate affinity had been formed due to intestinal adaptation of cholesterol load in diet. The present study demonstrated that cholesterol feeding caused a significant alteration in nutrients absorption, membrane enzymes and chemical composition of the small intestine. en_US
dc.language.iso en en_US
dc.publisher Karger Publishers en_US
dc.subject Cholesterol en_US
dc.subject Membrane vesicles en_US
dc.subject Nutrient transport en_US
dc.subject Hydrolytic enzymes en_US
dc.subject Biochemistry en_US
dc.title Enzymatic and transport studies in cholesterol fed guinea pigs using intestinal brush border membrane vesicle en_US
dc.type Journal Article en_US
dc.rights.holder S. Karger AG, Basel en_US


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