Browsing by Author "A. Anil Kumar"

Now showing 1 - 2 of 2

Insights into early generation synthetic amphidiploid Brassica juncea: a strategy to harness maximum parental genomic diversity for improving Indian mustard
(Frontiers Media SA, 2025) Pooja Garg; Shikha Tripathi; Anamika Kashyap; A. Anil Kumar; Sujata Kumari; Mandeep L. Singh; Ranjeet Kushwaha; Shivani Shankar Sharma; Jyoti Sharma; Rashmi P. Yadav; Navin Chandra Gupta; Naveen Singh; Ramcharan C. Bhattacharya; Vinod Chhokar; Mahesh Rao
In India, amphidiploid Brassica juncea (AABB, 2n=36) is a significant oilseed crop, but its small gene pool limits its ability to develop traits of higher breeding and economic value. Through interspecific hybridization from various lines of the progenitor species, resynthesized B. juncea (RBJ) can provide breeders with additional resources for creating genetically diverse stress-tolerant and high-yielding cultivars. Three B. rapa accessions and eight B. nigra accessions were crossed in this study to develop 33 synthetic B. juncea lines. A total of 28 crosses were attempted, including the three-way crosses, but only the cross combinations with B. rapa cytoplasm led to successful embryonic development. Molecular diversity analysis of these lines in S2 generation revealed significant genetic diversity with higher levels of heterozygosity and allelic richness, along with significant variations for the yield-related traits. These results suggest that the synthesized lines could effectively enrich the genetic base of B. juncea and generate variability for agronomically important traits in a shorter time duration. The characterized variability in the synthetic lines needs to be utilized in hybridization, with already evolved genotypes, in early generations before it is lost due to chromosomal rearrangements, recombination and natural selection. © © 2025 Garg, Tripathi, Kashyap, Anil Kumar, Kumari, Singh, Kushwaha, Sharma, Sharma, Yadav, Gupta, Singh, Bhattacharya, Chhokar and Rao.
Morphological, Histobiochemical and Molecular Characterisation of Low Lignin Phloem Fibre (llpf) Mutant of Dark Jute (Corchorus olitorius L.)
(Humana Press Inc., 2017) S.B. Choudhary; I. Chowdhury; R.K. Singh; S.P. Pandey; H.K. Sharma; A. Anil Kumar; P.G. Karmakar; N. Kumari; J. Souframanien; S.J. Jambhulkar
Lignin is a versatile plant metabolite challenging high-end industrial applications of several plant products including jute. Application of developmental mutant in regulation of lignification in jute may open up door for much awaited jute based diversified products. In the present study, a novel dark jute (Corchorus olitorius L.) mutant with low lignin (7.23%) in phloem fibre being compared to wild-type JRO 204 (13.7%) was identified and characterised. Unique morphological features including undulated stem, petiole and leaf vein distinguished the mutant in gamma ray irradiated mutant population. Histological and biochemical analysis revealed reduced lignification of phloem fibre cells of the plant. RT-PCR analysis demonstrated temporal transcriptional regulation of CCoAMT1 gene in the mutant. The mutant was found an extremely useful model to study phloem fibre developmental biology in the crop besides acting as a donor genetic stock for low lignin containing jute fibre in dark jute improvement programme. © 2017, Springer Science+Business Media New York.