Browsing by Author "A.N. Sahu"

Now showing 1 - 2 of 2

Encircling granulosa cells protects against di-(2-ethylhexyl)phthalate-induced apoptosis in rat oocytes cultured in vitro
(Cambridge University Press, 2019) Anima Tripathi; Vivek Pandey; A.N. Sahu; Alok K. Singh; Pawan K. Dubey
The present study investigated if the presence of encircling granulosa cells protected against di(2-ethylhexyl)phthalate (DEHP)-induced oxidative stress in rat oocytes cultured in vitro. Denuded oocytes and cumulus-oocyte complexes (COCs) were treated with or without various doses of DEHP (0.0, 25.0, 50.0, 100, 200, 400 and 800 μM) in vitro. Morphological apoptotic changes, levels of oxidative stress and reactive oxygen species (ROS), mitochondrial membrane potential, and expression levels of apoptotic markers (Bcl2, Bax, cytochrome c) were analyzed. Our results showed that DEHP induced morphological apoptotic changes in a dose-dependent manner in denuded oocytes cultured in vitro. The effective dose of DEHP (400 Âg) significantly (P>0.05) increased oxidative stress by elevating ROS levels and the mitochondrial membrane potential with higher mRNA expression and protein levels of apoptotic markers (Bax, cytochrome c). Encircling granulosa cells protected oocytes from DEHP-induced morphological changes, increased oxidative stress and ROS levels, as well as increased expression of apoptotic markers. Taken together our data suggested that encircling granulosa cells protected oocytes against DEHP-induced apoptosis and that the presence of granulosa cells could act positively towards the survival of oocytes under in vitro culture conditions and may be helpful during assisted reproductive technique programmes. © Cambridge University Press 2019.
Pharmacognostical and phytochemical evaluation of Cinnamomum wightii Meissn. flowers
(2012) D. Laloo; A.N. Sahu; S. Hemalatha; S.D. Dubey
Cinnamomum wightii Meissn. (Family- Lauraceae) is a tree mainly found in the Western Ghats and Shola forest of the South India. Pharmacognostical and phytochemical evaluation of crude drug has been done as per WHO guidelines and IHP 2002. The crude flower buds are globoid to round in shape, dark brown with 1-2 cm long pedicel. Transverse section through petals showed both the outer and inner epidermis with numerous stone cells and secretory glands scattered in the cortex. The quantitative values found are: foreign organic matter (0.71%), loss on drying (11.88%), total ash (6.41%), acid insoluble ash (2.07%), water soluble ash (0.38%), alcohol soluble extractive (28.63%) and water soluble extractive (7.23%), foaming index (200), swelling index (0.85 ml), volatile oil content (0.20%) and pesticide residue content. The powdered crude flower buds have shown the presence of stone cells, vessel element, covering trichomes, spiral xylem vessel and fragments of anthers filamentous cell layer. Phytochemical screening of methanolic extract showed the presence of alkaloids, glycosides, flavonoids, saponins, coumarins, reducing sugars, phenolics, tannins, sterols and mucilage. Quantitative estimation of the methanolic extracts of various phytoconstituents, viz. total tannins (156.5 mg/g), total phenolics (146.40 mg/g), total flavonoids (30 mg/g) and total flavonols (3.6 mg/g) content has been estimated spectrophotometrically. These findings may serve as diagnostic tools for identification of the crude drug.