Browsing by Author "Abhay Dev Tripathi"

Now showing 1 - 13 of 13

Advancements in Nano-Mediated Biosensors: Targeting Cancer Exosome Detection
(Springer, 2024) Abhay Dev Tripathi; Yamini Labh; Soumya Katiyar; Vivek K. Chaturvedi; Pooja Sharma; Abha Mishra
Cancer-derived exosomes, a subset of extracellular vesicles, carry vital information about tumor progression, metastasis, and drug resistance, making them attractive targets for cancer diagnostics and therapeutics. The identification of these cancer exosomes with high sensitivity and specificity has enormous promise for early diagnosis and prognosis. Nano-mediated biological sensors are establishing themselves as innovative techniques for detecting cancer exosomes based on the distinctive physicochemical attributes of nanomaterials to improve detection sensitivity and specificity. This article presents an overview of the recent developments in nano-mediated biosensors directed particularly toward the detection of cancer exosomes. The development of ultrasensitive sensors has been enhanced by using nanomaterials such as magnetic nanoparticles, quantum dots, and gold nanoparticles. Surface modifications of these nanomaterials by conjugating the cancer-specific antibodies or aptamers facilitate target recognition and binding of cancer exosomes, thus increasing the sensitivity of detection. This review compiles different detection techniques, including SERS, Electrochemical, SPR, Chemiluminescence, and Fluorescence-based biosensor detection, in combination with different nanomaterials that are currently being researched or utilized as biosensors. © The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2024.
Antimicrobial Proficiency of Amlodipine: Investigating its Impact on Pseudomonas spp. in Urinary Tract Infections
(Springer, 2024) Pooja Sharma; Aakanksha Kalra; Abhay Dev Tripathi; Vivek K. Chaturvedi; Bharti Chouhan
Antibiotic resistance in urinary tract infections (UTIs) is a growing concern due to extensive antibiotic use. The study explores a drug repurposing approach to find non-antibiotic drugs with antibacterial activity. In the present study, 8 strains of Pseudomonas spp. were used that were clinically isolated from UTI-infected patients. Amlodipine, a cardiovascular drug used in this study, has shown potential antimicrobial effect in reducing the various virulence factors, including swimming and twitching motility, biofilm, rhamnolipid, pyocyanin, and oxidative stress resistance against all the strains. Amlodipine exhibited the most potent antimicrobial activity with MIC in the range of 6.25 to 25 µg/ml. Significant inhibition in biofilm production was seen in the range of 45.75 to 76.70%. A maximum decrease of 54.66% and 59.45% in swimming and twitching motility was observed, respectively. Maximum inhibition of 65.87% of pyocyanin pigment was observed with the effect of amlodipine. Moreover, a significant decrease in rhamnolipids production observed after amlodipine treatment was between 16.5 and 0.001 mg/ml as compared to the control. All bacterial strains exhibited leakage of proteins and nucleic acids from their cell membranes when exposed to amlodipine which suggests the damage of the structural integrity. In conclusion, amlodipine exhibited good antimicrobial activity and can be used as a potential candidate to be repurposed for the treatment of urinary tract infections. © Association of Microbiologists of India 2024.
Biosynthesized nanoparticles: a novel approach for cancer therapeutics
(Frontiers Media SA, 2023) Vivek K. Chaturvedi; Bhaskar Sharma; Abhay Dev Tripathi; Dawesh P. Yadav; Kshitij RB Singh; Jay Singh; Ravindra Pratap Singh
Nanotechnology has become one of the most rapid, innovative, and adaptable sciences in modern science and cancer therapy. Traditional chemotherapy has limits owing to its non-specific nature and adverse side effects on healthy cells, and it remains a serious worldwide health issue. Because of their capacity to specifically target cancer cells and deliver therapeutic chemicals directly to them, nanoparticles have emerged as a viable strategy for cancer therapies. Nanomaterials disclose novel properties based on size, distribution, and shape. Biosynthesized or biogenic nanoparticles are a novel technique with anti-cancer capabilities, such as triggering apoptosis in cancer cells and slowing tumour growth. They may be configured to deliver medications or other therapies to specific cancer cells or tumour markers. Despite their potential, biosynthesized nanoparticles confront development obstacles such as a lack of standardisation in their synthesis and characterization, the possibility of toxicity, and their efficiency against various forms of cancer. The effectiveness and safety of biosynthesized nanoparticles must be further investigated, as well as the types of cancer they are most successful against. This review discusses the promise of biosynthesized nanoparticles as a novel approach for cancer therapeutics, as well as their mode of action and present barriers to their development. 2023 Chaturvedi, Sharma, Tripathi, Yadav, Singh, Singh and Singh.
Cadmium sulfide nanoparticles (CdSNPs) modulate key oncogenic pathways in PA1 ovarian cancer cells: Insights from transcriptomic analysis
(Elsevier Ltd, 2025) Aditi Bhatnagar; Abhay Dev Tripathi; Sonali Kumari; Abha Mishra
Transcriptomics has become a useful tool for comparing the levels of gene expression in healthy and malignant cells, holding potential for the discovery of new cancer therapies. This study used RNA-sequencing and transcriptome analysis on the PA1 ovarian cancer cell line to examine the potential of Cadmium Sulfide Nanoparticles (CdSNPs) as a therapeutic agent. A total of 5.42 Gb of high-quality reads was estimated based on the findings of gene expression techniques, comprising 2.25 Gb of treated PA1 cells and 3.17 Gb of control cells. Of these, 1641 genes with padj<0.001 and log2 foldchange >2 were found to be significantly regulated DEGs (differentially expressed genes). Analysis of gene ontology (GO) assays demonstrates the molecular mechanism behind CdSNPs anticancer effects. GO:0006915, GO:0012501, GO:1903561, and GO:0070588 are a few significant highlights of elevated GO (enriched DEGs) that are involved in apoptotic pathways, extracellular vesicles, programmed cell death, and Ca++ signaling. KEGG analysis elucidated that up and downregulated DEGs were enriched in a few pathways: calcium signaling pathway, Apoptosis, and TNF signaling pathway. Important pathways like MAP kinase, JAK/STAT, cAMP, and folate biosynthesis, showed inhibitory effects on ovarian cancer cell proliferation. The results of this work provide insight into possible therapeutic approaches employing CdSNPs and encourage additional research using a variety of cell lines and in vivo models to improve ovarian cancer treatment. © 2025
Folate-Mediated Targeting and Controlled Release: PLGA-Encapsulated Mesoporous Silica Nanoparticles Delivering Capecitabine to Pancreatic Tumor
(American Chemical Society, 2024) Abhay Dev Tripathi; Yamini Labh; Soumya Katiyar; Anurag Kumar Singh; Vivek K. Chaturvedi; Abha Mishra
The discovery of specifically tailored therapeutic delivery systems has sparked the interest of pharmaceutical researchers considering improved therapeutic effectiveness and fewer adverse effects. The current study concentrates on the design and characterization of PLGA (polylactic-co-glycolic acid) capped mesoporous silica nanoparticles (MSN)-based systems for drug delivery for pH-sensitive controlled drug release in order to achieve a targeted drug release inside the acidic tumor microenvironment. The physicochemical properties of the nanoformulations were analyzed using TEM, zeta potential, AFM, TGA, FTIR, and BET analyses in addition to DLS size. The final formed PLGA-FoA-MSN-CAP and pure MSN had sizes within the therapeutic ranges of 164.5 ± 1.8 and 110.7 ± 2.2, respectively. Morphological characterization (TEM and AFM) and elemental analysis (FTIR and XPS) confirmed the proper capping and tagging of PLGA and folic acid (FoA). The PLGA-coated FoA-MSN exhibited a pH-dependent controlled release of the CAP (capecitabine) drug, showing efficient release at pH 6.8. Furthermore, the in vitro MTT test on PANC1 and MIAPaCa-2 resulted in an IC50 value of 146.37 μg/ml and 105.90 μg/ml, respectively. Mitochondrial-mediated apoptosis was confirmed from the caspase-3 and annexin V/PI flow cytometry assay, which displayed a cell cycle arrest at the G1 phase. Overall, the results predicted that the designed nanoformulation is a potential therapeutic agent in treating pancreatic cancer. © 2024 American Chemical Society.
Graphene-silymarin-loaded chitosan/gelatin/hyaluronic acid hybrid constructs for advanced full-thickness burn wound management
(Elsevier B.V., 2024) Soumya Katiyar; Abhay Dev Tripathi; Ritika K. Singh; Avinash Kumar Chaurasia; Pradeep K. Srivastava; Abha Mishra
Burn wounds (BWs) with extensive blood loss, along with bacterial infections and poor healing, may become detrimental and pose significant rehabilitation obstacles in medical facilities. Therefore, the freeze-drying method synthesized novel hemocompatible chitosan, gelatin, and hyaluronic acid infused with graphene oxide-silymarin (CGH-SGO) hybrid constructs for application as a BW patch. Most significantly, synthesized hybrid constructs exhibited an interconnected-porous framework with precise pore sizes (≈118.52 µm) conducive to biological functions. Furthermore, the FTIR and XRD analyses document the constructs' physiochemical interactions. Similarly, enhanced swelling ratios, adequate WVTR (736 ± 78 g m−2 hr−1), and bio-degradation rates were seen during the physiological examination of constructs. Following the in vitro investigations, SMN-GO added to constructs improved their anti-bacterial (against E.coli and S. aureus), anti-oxidant, hemocompatible, and bio-compatible characteristics in conjunction with prolonged drug release. Furthermore, in vivo, implanting constructs on wounds exhibited significant acceleration in full-thickness burn wound (FT-BW) healing on the 14th day (CGH-SGO: 95 ± 2.1 %) in contrast with the control (Gauze: 71 ± 4.2 %). Additionally, contrary to gauze, the in vivo rat tail excision model administered with constructs assured immediate blood clotting. Therefore, CGH-SGO constructs with an improved porous framework, anti-bacterial activity, hemocompatibility, and biocompatibility could represent an attractive option for healing FT-BWs. © 2024
Herbal Drug-Loaded Nanoparticles for the Treatment of Neurodegenerative Diseases
(CRC Press, 2024) Soumya Katiyar; Shikha Kumari; Abhay Dev Tripathi; Ritika Singh; Pradeep K. Srivastava; Abha Mishra
Neurodegenerative disorders (NDs) such as Alzheimer’s disease, Parkinson’s disease, prion disease, spinal muscular atrophy, amyotrophic lateral sclerosis, Friedreich ataxia, and Huntington’s disease can severely affect or damage any brain areas. The exact cause and mechanism of NDs are unknown; however, various underlying molecular mechanisms and chemical processes have been proposed. Because of the presence of the blood-brain barrier (BBB), a tightly compacted system of blood arteries and endothelial cells that works to restrict the admittance of undesired substances into the brain, the approaches associated with the designing and advancement of new treatments for neurodegenerative illnesses are exceedingly complicated and demanding. Nanotechnology’s implementation and diverse progress offer promising potential for overcoming this issue. Nanotechnology has been one of the approaches that have changed human existence in numerous aspects and are an effective strategy that helps counteract the numerous restrictions of different illnesses, notably NDs. NPs can penetrate the BBB and deliver bioactive molecules to specific brain areas, reducing adverse reactions. Multiple therapeutic drugs might benefit from adding nanostructured materials such as polymeric/lipid nanoparticles (PNPs), nano-liposomes, nano-micelles, and carbon nanotubes (CNTs) to boost their effectiveness and performance along with minimizing adverse effects, prolonging the shelf life, and enhancing pharmacokinetics. The conjugates of nanoparticles (NPs) and traditional herbal plant bioactives (such as curcumin, berberine, quercetin, lycopene, thymoquinone, ferulic acid, and others) have recently gained importance in the creation of innovative neuro-therapeutics due to its universal availability, low cost, ability to allow potentially specific targeting of supply to the brain, and relatively low possibility for adverse outcomes. Furthermore, recent findings have shown that multiple plant-based compounds have remarkable neuroprotective, anti-oxidant, and reduced neuroinflammatory characteristics that help combat a wide range of NDs. This chapter discusses herbal therapeutics with remarkable potency in NDs, as well as documented herbal bioactive-loaded NP-based methods of delivery. © 2024 selection and editorial matter, Anurag Kumar Singh, Vivek K. Chaturvedi, and Jay Singh; individual chapters, the contributors.
In vitro and in vivo assessment of curcumin-quercetin loaded multi-layered 3D-nanofibroporous matrix prepared by solution blow-spinning for full-thickness burn wound healing
(Elsevier B.V., 2024) Soumya Katiyar; Divakar Singh; Abhay Dev Tripathi; Avinash Kumar Chaurasia; Ritika K. Singh; Pradeep K. Srivastava; Abha Mishra
Burn wounds (BWs) cause impairment of native skin tissue and may cause significant microbial infections that demand immediate care. Curcumin (Cur) and quercetin (Que) exhibit antimicrobial, hemocompatibility, ROS-scavenging, and anti-inflammatory properties. However, its instability, water insolubility, and low biological fluid absorption render it challenging to sustain local Cur and Que doses at the wound site. Therefore, to combat these limitations, we employed blow-spinning and freeze-drying to develop a multi-layered, Cur/Que-loaded gelatin/chitosan/PCL (GCP-Q/C) nanofibroporous (NFP) matrix. Morphological analysis of the NFP-matrix using SEM revealed a well-formed multi-layered structure. The FTIR and XRD plots demonstrated dual-bioactive incorporation and scaffold polymer interaction. Additionally, the GCP-Q/C matrix displayed high porosity (82.7 ± 2.07 %), adequate pore size (∼121 μm), enhanced water-uptake ability (∼675 % within 24 h), and satisfactory biodegradation. The scaffolds with bioactives had a long-term release, increased antioxidant activity, and were more effective against gram-positive (S. aureus) and gram-negative (E. coli) bacteria than the unloaded scaffolds. The in vitro findings of GCP-Q/C scaffolds showed promoted L929 cell growth and hemocompatibility. Additionally, an in vivo full-thickness BW investigation found that an implanted GCP-Q/C matrix stimulates rapid recuperation and tissue regeneration. In accordance with the findings, the Gel/Ch/PCL-Que/Cur NFP-matrix could represent an effective wound-healing dressing for BWs. © 2024 Elsevier B.V.
In Vitro Cytotoxic Assessment of Functionalized Multi-walled Carbon Nanotubes Against Cervical Cancer
(Springer, 2023) Vivek K. Chaturvedi; Abhay Dev Tripathi; Tarun Minocha; Veer Singh; Mohan P. Singh; Dawesh P. Yadav
Cervical cancer is the most prevalent reproductive malignancy that affects the female reproductive system. A myriad of anti-cancer compounds has been used for its treatment, but the increasing mortality rates are still devastating. Currently, organic metal complexes are gaining popularity due to their anti-cancer attributes. Therefore, the current work was designed to assess the therapeutic efficacy of non-functionalized multi-walled carbon nanotubes (N-MWCNT) and functionalized MWCNTs (F-MWCNT) against cervical cancer. Initially, N-MWCNTs and F-MWCNTs were structurally characterized by various parameters, including FTIR, XRD, TEM, SEM, AFM, and EDAX. Our comparative study revealed that F-MWCNTs have a larger surface area and the presence of OH and COOH as functional groups, which are uniformly distributed in the tubular and crystalline structure. Furthermore, the MTT assay revealed IC50 values of 47.70 µg/ml and 61.94 µg/ml of N- MWCNTs and F-MWCNT, respectively; in that relation, our immunoblot results show upregulation of BAX and downregulation of BCL-2 in either of the cases, with N-MWCNT having 2.92 and 0.45 folds in comparison to 2.68 and 0.41 folds of F-MWCNT. Our results revealed that both materials inhibited cell proliferation and induced apoptosis in a dose-dependent manner. Still, the effect of N-MWCNTs was more prominent as compared to F-MWCNTs. The overall conclusions of this study indicated that doses of F-MWCNTs could be explored for the safe drug delivery vehicle used as an effective biocompatible nanoformulation agent and this will open a new door for cervical cancer treatment. © 2023, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.
Mesoporous Silica Nanoparticles for Drug Delivery in Brain Disorders
(CRC Press, 2024) Abhay Dev Tripathi; Aditi Bhatnagar; Soumya Katiyar; Abha Mishra
The blood-brain barrier (BBB), a characteristic component of the central nervous system, effectively shields the brain from numerous blood-borne viruses and pathogens. The discovery of novel drugs for brain cancer and neurodegenerative diseases is hampered by this barrier, which also restricts treatment efficacy. The BBB’s extremely selective nature prevents all large-molecule therapies and over 98% of all small-molecule medicines from reaching the brain. Considering the lack of efficient formulations to treat brain-related disorders, nanotechnology offers various innovative design possibilities for novel therapeutics employing cutting-edge nanostructures for medication delivery. Mesoporous silica nanoparticles (MSNs) are one of the authentic nanocarriers for targeted drug release because of their easy functionalization, high surface area, biocompatibility, and structurally tunable properties that are possible with a low-cost synthesis process. They also stand out among the available nanosystems because they are non-hazardous to biological tissues. This chapter aims to provide an overview of MSN fabrication and the most recent developments regarding MSNs’ prospective use as brain-targeted drug delivery systems and evaluate their capabilities and benefits in overcoming these intriguing biological hurdles. © 2024 selection and editorial matter, Anurag Kumar Singh, Vivek K. Chaturvedi, and Jay Singh; individual chapters, the contributors.
Navigating Safety and Toxicity Challenges in Nanomedicine: Strategies, Assessment, and Mitigation
(Springer Science and Business Media B.V., 2024) Rajesh Kumar; Sunil Dutt; Abhay Dev Tripathi; Anurag Kumar Singh; Vivek K. Chaturvedi; Santosh Kumar Singh
The burgeoning field of nanomedicine offers unprecedented opportunities for targeted drug delivery, diagnostics, and imaging. However, alongside its promises, nanomedicine presents intricate safety and toxicity challenges that necessitate careful navigation. This abstract elucidates the multifaceted strategies, assessment methodologies, and mitigation approaches crucial for addressing safety concerns and realizing the potential of nanomedicine. Strategies aimed at enhancing safety encompass a spectrum of approaches, including surface modifications, formulation optimization, and the judicious selection of biocompatible materials. These strategies aim to minimize adverse effects while maximizing therapeutic efficacy by tailoring nanomaterials to interact harmoniously with biological systems. Additionally, the development of stimuli-responsive nanomaterials and intelligent drug delivery systems provides dynamic control over drug release, further enhancing safety profiles. Assessment methodologies play a pivotal role in evaluating the safety and toxicity of nanomedicines. Advanced analytical techniques, such as spectroscopy and imaging methods, offer insights into the physicochemical properties and interactions of nanomaterials within biological environments. In vitro and in vivo models facilitate systematic evaluation of biocompatibility, pharmacokinetics, and toxicity profiles, providing critical data for informed decision-making during preclinical and clinical development stages. Mitigation strategies are essential for managing and minimizing potential risks associated with nanomedicine toxicity. Robust regulatory frameworks, risk assessment protocols, and interdisciplinary collaborations foster transparency and ensure adherence to safety standards. Furthermore, the integration of predictive modeling and computational simulations enables the anticipation and mitigation of safety concerns early in the development process, enhancing overall safety profiles. In conclusion, navigating safety and toxicity challenges in nanomedicine necessitates a holistic approach integrating innovative strategies, rigorous assessment methodologies, and proactive mitigation measures. By fostering a comprehensive understanding of nanomaterial behavior and its interactions within biological systems, the field can harness its transformative potential while ensuring the highest standards of safety and efficacy in clinical applications. © The Author(s), under exclusive license to Springer Nature Switzerland AG 2024.
Porous silica and related composites for topical drug delivery
(Elsevier, 2025) Soumya Katiyar; Abhay Dev Tripathi; Shikha Kumari; Ritika Kumari Singh; Avinash Kumar Chaurasia; Pradeep Kumar Srivastava; Abha Mishra
Topical drug delivery (TDD) methods of silica nanocomposites (SCPs) and their composites have been extensively studied because of their appealing characteristics. These include high surface area, biocompatibility, thermal stability, tunable pore volume, and surface-active groups. The exclusive mesoporous arrangement of silica and the simplicity with which its surface may be modified render it possible to achieve efficient drug loading and regulated release at a designated spot. SCPs have recently been recognized as a possible therapeutic alternative to TDD systems for the treatment of a broad variety of medical conditions, encompassing malignancies (prostate, pancreatic, lung, skin, etc.), as well as tissue engineering and regenerative medicine. In this chapter we focus on the recent progress achieved in the synthesis of various SCPs and on the parameters that impact the unique characteristics and functions of these nanocomposites for TDD. Furthermore, a complete literature analysis will explain SCPs-based TDD transport, nonharmful effects, and biodistribution. The chapter also discusses the benefits and drawbacks of SCPs in terms of their current and potential medical applications in therapeutics. © 2026 Elsevier Inc. All rights reserved.
Retraction notice to “In vitro and in vivo assessment of curcumin-quercetin loaded multi-layered 3D-nanofibroporous matrix prepared by solution blow-spinning for full-thickness burn wound healing” [Int. J. Biol. Macromol. 270P1 (2024) 132269] (International Journal of Biological Macromolecules (2024) 270(P1), (S0141813024030745), (10.1016/j.ijbiomac.2024.132269))
(Elsevier B.V., 2025) Soumya Katiyar; Divakar Singh; Abhay Dev Tripathi; Avinash Kumar Chaurasia; Ritika Kumari Singh; Pradeep Kumar Srivastava; Abha Mishra
This article has been retracted: please see Elsevier Policy on Article Withdrawal (https://www.elsevier.com/locate/withdrawalpolicy). This article has been retracted at the request of the Editors-in-Chief. Concerns were raised about several instances of image duplications in Figs. 2, 7 and 9(a), where images seem to depict the same samples, but are labeled as different samples, with changes in focus and contrast. The corresponding author responded to these concerns but was unable to provide a satisfactory explanation and consequently the Editors-in-Chief decided to retract the article. © 2024 Elsevier B.V.