Browsing by Author "Aditya Sharma"

Now showing 1 - 8 of 8

Burden of Chronic Nonhealing Wounds: An Overview of the Worldwide Humanistic and Economic Burden to the Healthcare System
(SAGE Publications Inc., 2024) Aditya Sharma; Ravi Shankar; Ashish Kumar Yadav; Arvind Pratap; Mumtaz Ahmad Ansari; Vivek Srivastava
Chronic wounds have long been a significant public health concern, but the true impact of these wounds is unknown since research designs and measuring techniques vary, leading to inconsistent estimates. The definition of a wound is a loss of epithelial continuity caused by damage to the tissue. The following conditions can cause chronic wounds: panniculitis, pyoderma gangrenosum, traumatic, neurological, metabolic, hematologic, neoplastic, or infection-related. The growing global incidence of diabetes and the aging population necessitate greater attention to chronic wounds. Regrettably, it is sad that significant healthcare institutions have overlooked wound research. The study of health-related illnesses and occurrences in particular populations, including their distribution, frequency, and determinants, and the application of this research to control health problems. © The Author(s) 2024.
Cationic poly(lactic-co-glycolic acid) iron oxide microspheres for nucleic acid detection
(2013) Chandra Mouli Pandey; Aditya Sharma; Gajjala Sumana; Ida Tiwari; Bansi Dhar Malhotra
Herein, we envisage the possibility of preparing stable cationic poly(lactic-co-glycolic acid) (PLGA) microspheres encapsulating the iron oxide nanoparticles (IONPs; 8-12 nm). The IONPs are incorporated into PLGA in organic phase followed by microsphere formation and chitosan coating in aqueous medium via nano-emulsion technique. The average size of the microspheres, as determined by dynamic light scattering are about 310 nm, while the zeta potential for the composite remains near 35 mV at pH 4.0. These microspheres are electrophoretically deposited onto indium tin oxide (ITO)-coated glass substrate used as cathode and parallel platinum plate as the counter electrode. This platform is utilized to fabricate a DNA biosensor, by immobilizing a probe sequence specific to Escherichia coli. The bioelectrode shows a surface-controlled electrode reaction with the electron transfer coefficient (α) of 0.64 and charge transfer rate constant (ks) of 61.73 s-1. Under the optimal conditions, this biosensor shows a detection limit of 8.7 × 10-14 M and is found to retain about 81% of the initial activity after 9 cycles of use. © 2013 The Royal Society of Chemistry.
Enhanced indole-3-acetic acid production by Enterobacter hormaechei APSB3 through heuristic artificial neural network and particle swarm optimisation
(Springer, 2025) Aditya Sharma; Guddu Kumar Gupta; Deepak Chhabra; Piyush Pandey; Pratyoosh Shukla
Indole-3-acetic acid (IAA) is essential in abiotic stress tolerance via signalling molecules between plants and microorganisms, contributing to sustainable agricultural practices. The present study investigates a combinatorial artificial neural network (ANN) modelling and particle swarm optimisation (PSO) algorithm to optimise the process parameters for enhanced IAA production by Enterobacter hormaechei APSB3. Hence, to improve IAA production, single-factor experiments and a design matrix generated by central composite design were employed to explore the significant input variables, including temperature, pH, carbon source, and nitrogen source, which were subsequently validated through the application of ANN-PSO. Thus, under the optimised ANN-PSO conditions, i.e. carbon source (2.11%), nitrogen source (2.37%), pH (9), and temperature (45 ℃), IAA production was improved to 94.76 ± 0.03 µg/mL (2.90-fold) as compared to un-optimised condition (33.04 ± 0.58 µg/mL). The IAA production was further confirmed by TLC and HPLC analyses, exhibiting an Rf value of 0.77 and a retention time of 3.301 min. Thus, the present work could conclude that the hybrid heuristic ANN-PSO, an empirical and decision-making tool, significantly improves efficiency and scalability for IAA production by E. hormaechei APSB3. © The Author(s) under exclusive licence to Society for Environmental Sustainability 2025.
Epidemiological profile of chronic wounds in a rural and urban community of an Indian district: A feasibility study trial
(Wolters Kluwer Medknow Publications, 2023) Aditya Sharma
[No abstract available]
Molecular insights and omics-based understanding of plant–microbe interactions under drought stress
(Springer Science and Business Media B.V., 2024) Aditya Sharma; Prassan Choudhary; Hillol Chakdar; Pratyoosh Shukla
The detrimental effects of adverse environmental conditions are always challenging and remain a major concern for plant development and production worldwide. Plants deal with such constraints by physiological, biochemical, and morphological adaptations as well as acquiring mutual support of beneficial microorganisms. As many stress-responsive traits of plants are influenced by microbial activities, plants have developed a sophisticated interaction with microbes to cope with adverse environmental conditions. The production of numerous bioactive metabolites by rhizospheric, endo-, or epiphytic microorganisms can directly or indirectly alter the root system architecture, foliage production, and defense responses. Although plant–microbe interactions have been shown to improve nutrient uptake and stress resilience in plants, the underlying mechanisms are not fully understood. “Multi-omics” application supported by genomics, transcriptomics, and metabolomics has been quite useful to investigate and understand the biochemical, physiological, and molecular aspects of plant–microbe interactions under drought stress conditions. The present review explores various microbe-mediated mechanisms for drought stress resilience in plants. In addition, plant adaptation to drought stress is discussed, and insights into the latest molecular techniques and approaches available to improve drought-stress resilience are provided. © 2023, The Author(s), under exclusive licence to Springer Nature B.V.
Plant-microbiome responses under drought stress and their metabolite-mediated interactions towards enhanced crop resilience
(Elsevier B.V., 2025) Aditya Sharma; Nandita Das; Piyush Pandey; Pratyoosh Shukla
The impacts of climate change are felt worldwide; however, drought stress poses significant challenges to global agriculture, affecting crop yields and food security. Understanding the multifaceted responses of crop plants to drought, particularly through their interaction with microbiomes and metabolites, is crucial and urgent for developing resilient agricultural systems. This review highlights the detrimental effects of drought on crop plants, including reduced water use efficiency, the production of free radicals, impaired plant growth and yield, and alterations in the photosynthetic apparatus. Additionally, this review addresses the research progress on plant responses, microbiome assemblages, metabolomic responses, and interactions under drought stress. By integrating findings from metabolomics, we discuss the “call for help” signal via root exudates in crop plants and their microbiomes during drought stress. Key aspects include the reciprocal exchange of metabolites (oxaloacetic acid, flavonoids, triterpenoids, phytoalexin, coumarin, and pyruvic acid), osmoprotectants (proline, sugars, amino acids), antioxidant enzymes (peroxidase, catalase, superoxide dismutase), and phytohormones (salicylic acid, jasmonic acid, and abscisic acid), along with the activation of stress-responsive pathways. Here, we explain the forefront of deciphering plant-microbiome interactions using cutting-edge metabolomics techniques. Therefore, this review summarizes the significance of metabolic and chemical exchanges between coexisting microorganisms to combat the escalating challenges of drought conditions effectively. © 2025 The Authors
Role of Bovine Colostrum Dressing on Chronic Non-Healing Wounds in Comparison to Conventional Dressing: A Case-Control Study
(SAGE Publications Inc., 2024) Vikas Mandloi; Tuhina Banerjee; Aditya Sharma; Arvind Pratap; Mumtaz Ahmad Ansari; Vivek Srivastava
Colostrum has been shown to be suitable for oral and/or topical applications. Colostrum decreases the amount of discharge from wounds and also accelerates healing, leading to a decrease in the number of dressings. In this study, 40 patients with chronic non-healing wounds were divided into two groups, considering the inclusion and exclusion criteria. Group I included 15 patients with conventional dressings, and Group II included 25 patients with added topical colostrum dressings. All patients were assessed at the time of presentation and after 21 days. The results of the present study indicate that colostrum powder dressings may be used as an adjunct in the management of chronic non-healing wounds. © The Author(s) 2024.
Significance of Plant Growth-Promoting Rhizobacteria in Alleviating Drought Stress in Crop Plants Under a Changing Climate for Sustainable Agricultural Production
(Springer, 2025) Aditya Sharma; Pratyoosh Shukla; Piyush Pandey
The intensification of drought and climate change presents a significant risk to agricultural production, increasing the urgency of finding environmentally friendly solutions to ensure global food security. Drought stress altered the stomatal conductance, osmotic adjustment, antioxidant, root architecture and morphology, regulation of hormonal signaling, metabolic regulations, and molecular responses in crop plants. In this context, plant growth-promoting rhizobacteria (PGPR) has emerged as a crucial sustainable agricultural solution in a changing climate. These bacteria significantly alleviate drought stress in crop plants through various mechanisms, including modifying root morphology, regulating stress-responsive genes, influencing plant hormones and stress signalling pathways, and producing volatile organic compounds and exopolysaccharides. Furthermore, PGPR improves plant resilience to drought by enhancing water and nutrient uptake efficiency, modulating stress hormone levels, and inducing systemic resistance mechanisms. This chapter explores the sophisticated roles of PGPR in mitigating drought stress, highlighting recent advances in understanding their interactions with crop plants under changing climatic conditions. Additionally, it discusses practical applications of PGPR-based strategies for sustainable agriculture production. It emphasizes their potential to enhance crop yields, reduce environmental impact, and promote resilience in agricultural systems vulnerable to drought. © The Author(s), under exclusive license to Springer Nature Singapore Pte Ltd. 2025.