Browsing by Author "Ajai Kumar Singh"

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Characterization and identification of pigeonpea [cajanus cajan (L.) millsp.] genotypes based on quality of seed protein
(Agricultural Research Communication Centre, 2020) Ashish Kumar Pandey; Ajai Kumar Singh; Prakash Singh; Rajendra Prasad Vyas; Ravish Kumar Singh; Hemraj Bhandari
Tris-and water-soluble seed protein profiling was used to characterize, identify and differentiate 13 genotypes of pigeonpea via SDS– PAGE based electrophoresis of seed protein. Electropherograms, RM-value and UPGMA based cluster (dendrograms) analysis was used to analyze tris-and water-soluble protein banding patterns of these genotypes. It revealed that RM-value of protein bands have differed from all the genotypes for both soluble proteins. Tris-soluble protein banding pattern exhibited 17 bands including 12-15 variate of RM-value (0.083 to 0.98) and two common bands with RM-value 0.083 and 0.78. However, water-soluble protein banding pattern revealed the presence of 16 bands including 8-12 variate of RM-value (0.16 to 0.95) with four common bands of RM-value 0.43, 048, 0.23 and 0.65, respectively. UPGMA cluster analysis was used to group the 13 genotypes into six distinct clusters. The banding pattern of tris-soluble protein was found to be more distinct than water-soluble protein. The result demonstrated that the electrophoretic profile of tris-soluble protein through SDS-PAGE was more effective than the water-soluble protein. © 2020, Agricultural Research Communication Centre. All rights reserved.
Toxic effects of sodium arsenate (Na2HAsO4· 7H2O) on the skin epidermis of air-breathing catfish Clarias batrachus (L.)
(2008) Ajai Kumar Singh; Tarun Kumar Banerjee
The toxicopathological effects have been investigated of a sublethal concentration (1 ppm) of sodium arsenate on the epidermis of the skin of air-breathing catfish Clarias batrachus L. The skin that acts as an accessory respiratory organ in this fish, faces direct contact stress of the toxicants and exhibits extensive damage, including massive wear and tear, sloughing of the epithelial cells (ECs) along with degeneration of the club cells (CCs) whose contents get squeezed out onto the body surface. This causes altered histomorphology of the epidermis. The mucous cells (MCs) show great hyperplasia and hypertrophy at most exposure periods. The staining properties of MCs also showed periodic alterations exhibiting more affinity for sulphate moieties. A thick layer of slime very often protects the surface of the skin. The epidermis also exhibits periodic but independent fluctuations in its protein, RNA and DNA contents. This is due to periodic synthesis, accumulation and sloughing of the slime, along with degeneration followed by regeneration of its different cellular elements, especially in the earlier stages of the treatment.