Browsing by Author "Alakh N. Sahu"

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A comparative analysis of phytochemicals versus synthetic drugs/nanomedicines in the treatment of uterine fibroid: a systematic review
(Korean Society of Environmental Risk Assessment and Health Science, 2024) Sonal Upadhyay; Vivek Pandey; Anima Tripathi; Alakh N. Sahu; Anjali Rani; Amita Diwakar; Lavina Chaubey; Rashmi Gupta; Pawan K. Dubey
Most women experience uterine fibroids (UFs), a common benign gynecological tumor, at some point in their reproductive age. There are several pharmacological treatments available to shrink fibroids and lessen the UF symptoms. These medications cost a lot of money, though, and frequently have serious side effects. Therefore, due to their low cost, comparable and powerful therapeutic efficiency and lower side effects, phytochemical-based medications are gaining popularity in these days. This review's goal is to provide a summary of the knowledge that is currently unavailable regarding the mechanisms of the action of various phytochemical-based medications with anti-uterine fibroid efficacy. The present results showed that dietary phytocompounds (dehydroxyelephantopin, butein, capsaicin, fisetin, kaempferol, resveratrol, silibinin and curcumin) could probably be effective as therapeutic compounds for uterine leiomyoma. These phytochemicals indicated their capability to regulate main fibroid promoting and initiating events for instance, proliferation, inflammation, angiogenesis and fibrosis in various experimental setups through modulating various signaling pathways, such as Smad 2/3, PI3K/AKT/mTOR, ERK 1/2 and β-catenin indicating that they could serve as targets for prevention and/or treatment of UFs. This review provides key molecular insights for the development of phytochemical-based novel personalized therapy for non-surgical management of UFs which may help to avoid hysterectomy. © The Author(s), under exclusive licence to Korean Society of Environmental Risk Assessment and Health Science 2023.
A phytopharmacological review on alternanthera ficoidea
(Advanced Scientific Research, 2019) Stuti Pandey; Ajay K. Pal; Alakh N. Sahu; Manmath K. Nandi
The spine of Ayurveda and other traditional system of medicines is medicinal plants. The medicinal plants and their extracts are rich with minerals, primary metabolites and secondary metabolites, which are the effective against various diseases. India has big biodiversity that rich with medicinal plants and near about 2500 medicinal plants of Indian origin are recommended in traditional treatment for various diseases. In recent decades metabolites derived from the extract of medicinal plants are frequently used by allopathic physicians as cardio-vascular, immunomodulatory, anticancer and antibiotic medicines. One of the Indian medicinal plant, Alternanthera ficoidea (L.) (Synonyms: Alternanthera tenella Colla and Gomphera ficoidea) belongs to family Amaranthaceae. The plant is traditionally used as diuretic, anti-pyretic, antiseptic, anti-inflammatory and anti- diarrheal agent etc. The secondary metabolites like tannins, saponins, phytols, carotenoids and polyphenols are major classes of compounds are identified for the plant. Some phytomolecules are isolated from the extract as vitexin, quercetin, kaempferol, amarantin, betaine, isoamarantin and sterol etc. Pharmacological activities like hypolipidemic, anti-inflammatory, antidiabetic, antibacterial and immunomodulatory activity are also investigated on this plant. This study compile the information related to Alternanthera ficoidea, which will helpful the researchers for further study on this plant. © 2019, Advanced Scientific Research. All rights reserved.
Asparagus racemosus root-derived carbon nanodots as a nano-probe for biomedical applications
(Springer, 2022) Gaurav Gopal Naik; Tarun Minocha; Abhineet Verma; Sanjeev Kumar Yadav; Satyen Saha; Ashish Kumar Agrawal; Sanjay Singh; Alakh N. Sahu
Nowadays, green nanotechnology has emerged as a crucial and eco-friendly approach to combat the problems affecting human health or the environment. It deals with the extensive utilization of plants owing to their cocktail of natural and chemical substances to eliminate adverse pollutants involved in the synthesis of nanomaterials. Herein, we report a facile one-step hydrothermal carbonization approach synthesizing fluorescent bluish-green carbon nanodots (CNDs) using Asparagus racemosus roots termed ARCD. The fluorescence quantum yield of ARCD was further improved by surface passivating it with a 1:1 ratio of Carrageenan (CAR) and Polyethylenimine (PEI), termed ARCCD. First, we demonstrate the application of ARCD and ARCCD as a nano-probe for sensing As3+ and Ag+. Second, we show an inhibitory effect on cancer cell proliferation against breast (MDA-MB-231) and cervical (SiHa) cancer. Also, no significant cytotoxicity was observed in the normal kidney (HEK 293) cells, indicating cytotoxic specificity of the developed CNDs. Third, we demonstrate antibacterial activity against clinically isolated multi-drug-resistant strains and free radicals scavenging potential. Next, we present the in vivo toxicity evaluation of these CNDs in Swiss albino mice, which exhibited minimal toxicity as confirmed by biochemical, hematological, and histological parameters. This study deals with an innovative approach to constructing a multifunctional nano-probe for potential biomedical and environmental applications. Graphical abstract: [Figure not available: see fulltext.]. © 2022, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.
Bioengineered dual fluorescent carbon nano dots from Indian long pepper leaves for multifaceted environmental and health utilities
(Springer Science and Business Media Deutschland GmbH, 2023) Debadatta Mohapatra; Ravi Pratap; Vivek Pandey; Singh Shreya; Gaurav Gopal Naik; Subhash C. Mandal; Sunday O. Otimenyin; Pawan K. Dubey; Avanish S. Parmar; Alakh N. Sahu
In this article, we present the synthesis of Piper longum leaves–derived ethanolic carbon dots (PLECDs) using the most simplistic environmentally friendly solvothermal carbonization method. The PLECDs fluoresced pink color with maximum emission at 670 nm at 397 nm excitation. Additionally, the dried PLECDs dissolved in water showed green fluorescence with higher emission at 452 nm at 370 nm excitation. The UV spectra showed peaks in the UV region (271.25 nm and 320.79 nm) and a noticeable tail in the visible region, signifying the efficient synthesis of nano-sized carbon particles and the Mie scattering effect. Various functional groups (–OH, –N–H, –C–H, –C = C, –C–N, and –C–O) were identified using Fourier transform infrared spectroscopy (FTIR). Its nanocrystalline property was revealed by the sharp peaks in the X-ray diffraction (XRD). High-resolution transmission electron microscopy (HRTEM) photomicrograph displayed a roughly spherical structure with a mean size of 2.835 nm. The energy dispersive X-ray (EDAX) and X-ray photoelectron spectroscopy (XPS) revealed the elemental abundance of C, O, and N. The high-performance thin-layer chromatography (HPTLC) fingerprint of PLECDs showed an altered pattern than its precursor (Piper longum leaves ethanolic extract or PLLEE). The PLECDs sensed Cu2+ selectively with a limit of detection (LOD) and limit of quantification (LOQ) of 0.063 μM and 0.193 μM, respectively. It showed excellent cytotoxicity toward MDA-MB-231 (human breast cancer), SiHa (human cervical carcinoma), and B16F10 (murine melanoma) cell lines with excellent in vitro bioimaging outcomes. It also has free radical scavenging activity. The PLECDs also showed outstanding bacterial biocompatibility, pH-dependent fluorescence stability, photostability, physicochemical stability, and thermal stability. © 2023, The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.
Carbon dots from an immunomodulatory plant for cancer cell imaging, free radical scavenging and metal sensing applications
(Future Medicine Ltd., 2021) Debadatta Mohapatra; Md. Bayazeed Alam; Vivek Pandey; Ravi Pratap; Pawan K. Dubey; Avanish S. Parmar; Alakh N. Sahu
Aim: This work aimed to develop Tinospora cordifolia stem-derived carbon dots (TCSCD) for cancer cell imaging, free radical scavenging and metal sensing applications. Method: The TCSCDs were synthesized by a simple, one-step, and ecofriendly hydrothermal carbonization method and characterized for their optical properties, morphology, hydrodynamic size, surface functionality, crystallinity, stability, bacterial biocompatibility, in vitro cellular imaging, free radical scavenging and metal sensing ability. Results: The TCSCDs exhibited excellent biocompatibility with dose-dependent bioimaging results in melanoma (B16F10) and cervical cancer (SiHa) cell lines. They exerted good free radical scavenging, Fe3+ sensing, bacterial biocompatibility, photostability, colloidal dispersion stability and thermal stability. Conclusion: The results reflect the potential of TCSCDs for biomedical and pharmaceutical applications. © 2021
Cryptocoryne spiralis, a substitute of Aconitum heterophyllum in the treatment of diarrhoea
(Oxford University Press, 2014) Satyendra K. Prasad; Damiki Laloo; Alakh N. Sahu; Gopal Nath; Siva Hemalatha
Objective To scientifically validate the traditional substitution of roots of highly expensive Aconitum heterophyllum (AH) with rhizomes of Cryptocoryne spiralis (CS) in the treatment of diarrhoea. Methods Different fractions from root/rhizome extract of both the plant were subjected to faecal excretion rate and castor oil-induced diarrhoea models. Further, bioactive fractions from both plants, i.e. chloroform (CAH) from AH at 50 mg/kg p.o. and ethyl acetate (EACS) from CS at 100 mg/kg p.o., were examined for small intestinal transit, intestinal fluid accumulation and PGE2-induced enteropooling models in rats. Biochemical estimations and Na+ and K+ concentration in intestinal fluid were also determined along with antibacterial studies. Phytochemical standardisation of AH and CS was performed by quantifying aconitine for the former and stigmasterol for the latter using HPLC. Key findings CAH and EACS illustrated a significant reduction in faecal output rate and demonstrated a protection of 63.068% at CAH 50 and 59.090% at EACS 100 mg/kg p.o. in castor oil-induced diarrhoea model. The fractions also persuaded promising effects in all the other models, restored alterations in biochemical parameters and showed potential antibacterial activity. Conclusion The antidiarrhoeal potential of AH and CS may be attributed to an antimotility and antisecretory type of effect. © 2014 Oxford University Press. All rights reserved.
Di-(2-ethylhexyl) phthalate (DEHP) inhibits steroidogenesis and induces mitochondria-ROS mediated apoptosis in rat ovarian granulosa cells
(Royal Society of Chemistry, 2019) Anima Tripathi; Vivek Pandey; Alakh N. Sahu; Alok Singh; Pawan K. Dubey
Increased oxidative stress (OS) due to ubiquitous exposure to di-(2-ethylhexyl) phthalate (DEHP) can affect the quality of oocytes by inducing apoptosis and hampering granulosa cell mediated steroidogenesis. This study was carried out to investigate whether DEHP induced OS affects steroidogenesis and induces apoptosis in rat ovarian granulosa cells. OS was induced by exposing granulosa cells to various concentrations of DEHP (0.0, 100, 200, 400 and 800 μM) for 72 h in vitro. Intracellular reactive oxygen species (ROS), oxidative stress (OS), mitochondrial membrane potential, cellular senescence, apoptosis, steroid hormones (estradiol & progesterone) and gene expression were analyzed. The results showed that an effective dose of DEHP (400 μg) significantly increased OS by elevating the ROS level, mitochondrial membrane potential, and β-galactosidase activity with higher mRNA expression levels of apoptotic genes (Bax, cytochrome-c and caspase3) and a lower level of an anti-apoptotic gene (Bcl2) as compared to the control. Further, DEHP significantly (P > 0.05) decreased the level of steroid hormones (estradiol and progesterone) in a conditioned medium and this effect was reciprocated with a lower expression (P > 0.05) of steroidogenic responsive genes (Cyp11a1, Cyp19A1, Star, ERβ1) in treated granulosa cells. Furthermore, co-treatment with N-Acetyl-Cysteine (NAC) rescues the effects of DEHP on OS, ROS, β-galactosidase levels and gene expression activities. Altogether, these results suggest that DEHP induces oxidative stress via ROS generation and inhibits steroid synthesis via modulating steroidogenic responsive genes, which leads to the induction of apoptosis through the activation of Bax/Bcl-2-cytochrome-c and the caspase-3-mediated mitochondrial apoptotic pathway in rat granulosa cells. © 2019 The Royal Society of Chemistry and the Centre National de la Recherche Scientifique.
In Vitro Cancer Cell Imaging, Free Radical Scavenging, and Fe3+ Sensing Activity of Green Synthesized Carbon Dots from Leaves of Piper longum
(Springer, 2023) Debadatta Mohapatra; Ravi Pratap; Vivek Pandey; Singh Shreya; Prakash Ch. Senapati; Pawan K. Dubey; Avanish S. Parmar; Alakh N. Sahu
The development of carbon dots via a green synthesis approach from natural products is one of the most researched areas nowadays. Herein, we present the synthesis of Piper longum leaves-derived aqueous carbon dots (PLACDs) via the simplest ecofriendly hydrothermal carbonization method. The PLACDs exhibited excitation-dependent emission behavior with maximum emission at 450 nm at an excitation wavelength of 365 nm. The High-Resolution Transmission Electron Microscopy results showed a quasi-spherical shape with an average size of 4.121 nm. The sharp diffractions of X-ray diffraction revealed its nanocrystalline property. The Energy Dispersive X-ray spectra reflected the presence of carbon, nitrogen, and oxygen. The Fourier-Transform Infrared Spectroscopy disclosed the existence of –OH, –C=C, –C=O, and –C–O–C groups. The PLACDs presented excellent biocompatibility against B16F10 (melanoma) and SiHa (cervical carcinoma) cells lines with concentration-dependent in vitro bioimaging results. It also exhibited antiradical activity with the IC50 value of 0.499 mg/mL and 0.051 mg/mL against DPPH and ABTS.+, respectively. It showed Fe3+ sensing with a lower limit of detection of 0.673 μM. Further, the PLACDs displayed excellent bacterial biocompatibility, pH-dependent fluorescence property, fluorescent ink property, photostability, physical, chemical, and thermal stability. © 2022, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.
Multi-Functional Carbon Dots from an Ayurvedic Medicinal Plant for Cancer Cell Bioimaging Applications
(Springer, 2020) Gaurav Gopal Naik; Md. Bayazeed Alam; Vivek Pandey; Debadatta Mohapatra; Pawan K. Dubey; Avanish S. Parmar; Alakh N. Sahu
The combination of an Ayurvedic wisdom and nanotechnology may help us to resolve the complex healthcare challenges. A facile and economical one-pot hydrothermal synthesis method has been adopted for preparing a blue fluorescent carbon dots (CDs) with a quantum yield of 15.10% from an Ayurvedic medicinal plant Andrographis paniculata (AP). The Andrographis paniculata derived CDs (AAPCDs) were then characterized using different techniques. Through High Performance Thin Layer Chromatography (HPTLC) profiling of the AP extract and the CDs, it was found that some of the phytoconstituents are retained as such while others may have been converted into their derivatives during the process of formation of CDs. The CDs are designed to possess cellular imaging of human breast carcinoma cells (MCF-7), apart from free radicals sensing and scavenging capabilities. AAPCDs showed minimal cytotoxicity in Multi Drug Resistant clinically isolated strains of gram positive and gram negative bacteria which may be employed for microbiology oriented experiments. These results suggest potential of multi-functional AAPCDs as nano-probes for various pharmaceutical, biomedical and bioengineering applications. © 2020, Springer Science+Business Media, LLC, part of Springer Nature.
Pink Fluorescent Carbon Dots Derived from the Phytomedicine for Breast Cancer Cell Imaging
(Wiley-Blackwell, 2020) Gaurav Gopal Naik; Md Bayazeed Alam; Vivek Pandey; Pawan Kumar Dubey; Avanish S. Parmar; Alakh N. Sahu
Phytomedicines have been beneficial to humankind for the treatment of implacable ailments from time immemorial. In this report, we have synthesized a carbon dots as a photomedicine from traditional phytomedicine Andrographis paniculata which is widely spread across Southern and Southeastern Asia. Interestingly, these carbon dots exhibited unique pink fluorescence that is perceptibly distinctive from the previous reports. The inherent properties of carbon dots were characterised using Transmission Electron Microscopy, Fluorescence spectroscopy, Absorption spectroscopy, Fourier transform infrared spectroscopy, Selected area diffraction, X-Ray Diffraction, and Energy Dispersive X-ray Spectroscopy. Furthermore, thermal stability of carbon dots was determined using Thermo gravimetric analysis. The carbon dots are fabricated to possess breast cancer cell bioimaging applications apart from anti-bacterial activities against clinically isolated strains of gram-positive and gram-negative bacteria and free radicals sensing and scavenging attributes. These findings establish carbon dots as a potential photomedicine with wide healthcare applications. © 2020 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim
Shatavarin-IV rescues the Di (2-ethylhexyl) phthalate (DEHP) induced oxidative stress in rat granulosa cells in vitro
(Elsevier Inc., 2024) Vivek Pandey; Alka Sharma; Sonal Tiwari; Yashvant Patel; Jayhind Kumar Chauhan; Safiya Ayesha; Alakh N. Sahu; Rashmi Gupta; Anima Tripathi; Pawan K. Dubey
Studies provide notable evidence that oxidative stress (OS) mediated reactive oxygen species (ROS) disturb reproductive health. We have shown in our previous publication that exposure of Di-(2-ethylhexyl) phthalate (DEHP), induces OS mediated ROS generation which inhibits steroid synthesis. In the present study, we demonstrated the ameliorative/protective effects of one of the steroidal saponins, i.e., Shatavarin-IV, isolated from the roots of Asparagus racemosus against DEHP induced OS in rat granulosa cells. Granulosa cells were exposed with DEHP alone (400 μM), Shatavarin-IV alone (8 μg/ml), and a combination of DEHP + Shatavarin-IV (400 μM + 8 μg/ml) in vitro for 24 hrs. Intracellular ROS, OS/hypoxia, mitochondrial membrane potential, steroid-responsive genes expression were analyzed. The results revealed that the effective dose of DEHP (400 µg) significantly increased OS compared to the control by increasing ROS levels, mitochondrial membrane potential, and β-galactosidase activity with a higher level of apoptotic genes (Bax, Caspase-3) expression at mRNA level. Further, DEHP significantly (p < 0.05) reduced mRNA expression of steroidogenic responsive genes (StAR, CYP17A1, and CYP19A1) in granulosa cells treated with above combination compared to control. Interestingly, co-treatment of DEHP + Shatavarin-IV significantly suppressed the DEHP induced OS, ROS, β-galactosidase levels and enhanced steroidogeneic and apoptotic gene expression activities, which suggests that Shatavarin-IV rescued DEHP-induced changes that may useful for the prevention of DEHP- induced reproductive toxicity. © 2024 Elsevier Inc.
Tinospora cordifolia Leaves Derived Carbon dots for Cancer Cell Bioimaging, Free radical Scavenging, and Fe3+ Sensing Applications
(Springer, 2022) Debadatta Mohapatra; Ravi Pratap; Vivek Pandey; Pawan K. Dubey; Ashish K. Agrawal; Avanish S. Parmar; Alakh N. Sahu
Herein, we report the fabrication of Tinospora cordifolia leaves-derived carbon dots (TCLCDs) from aqueous extract of leaves as carbon source via simple, environmentally friendly, hydrothermal carbonization (HTC) technique. The synthesized TCLCDs were characterized for their physicochemical properties and further explored for in-vitro cancer cell bioimaging, radical scavenging, and metal ion sensing. The synthesized TCLCDs showed excitation-dependent emission property with maximum emission at 435 nm under the excitation of 350 nm. The High-Resolution Transmission Electron Microscopy (HRTEM) results revealed a roughly spherical shape with an average diameter of 5.47 nm. The diffused ring pattern of Selected Area Electron Diffraction (SAED) and halo diffraction pattern of X-ray diffraction (XRD) disclosed their amorphous nature. The Energy Dispersive X-ray (EDX) showed the existence of C, N, and O. The Fourier-transform infrared spectroscopy (FTIR) revealed the presence of -OH, -NH, -CN, and -CH groups. The TCLCDs showed excellent cellular biocompatibility with dose-dependent bioimaging results in melanoma (B16F10) and cervical cancer (SiHa) cell lines. Also, they exhibited excellent scavenging of free radicals with an IC50 value of 0.524 mg/mL & selective Fe3+ ion sensing with a detection limit of 0.414 µM. Further, they exerted excellent bacterial biocompatibility, photostability, and thermal stability. The overall results reflected their potential for in-vitro cancer cell bioimaging, free radical scavenging, and selective Fe3+ ion sensing. © 2021, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.