Browsing by Author "Amin Golshah"

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Applying the Taguchi method to the optimization of anticancer activity of bacterial alginate-CuO bionanocomposite
(Open Access Macedonian Journal of Medical Sciences, 2019) Amin Golshah; Mojtaba Taran; Mohsen Safaei; Hamid Reza Mozaffari; Mohammad Moslem Imani; Roohollah Sharifi; Hedaiat Moradpoor; Prabhat Upadhyay
AIM: In recent decades, despite various types of cancer inflicting many people worldwide, the existing therapies are not satisfactory and have many side effects. The present study was conducted to optimise the synthesis of novel alginate-CuO nanocomposite with utmost anticancer activity. METHODS: In this study, 9 nanocomposites were designed using Taguchi method and three factors including copper oxide nanoparticles, alginate biopolymer and stirring times were assessed at three different levels. The anticancer activity of the synthesised nanocomposites was evaluated on the MCF-7 cell line using the MTT method. Using the Qulitek-4 software, we determined the optimum conditions for the synthesis of alginate-CuO nanocomposite with the highest anticancer activity. RESULTS: The results indicated that all three factors (copper oxide, alginate and stirring time) were effective on the anticancer activity of the alginate-CuO nanocomposite. Also, the nanocomposite produced under the conditions of experiment 9 (8 mg/ml of copper oxide, 2 mg/ml of alginate and 60 min of stirring time) provided the highest growth inhibition rate as 75.63% against cancer cells. CONCLUSION: The synthesised alginate-copper oxide nanocomposites in this study showed a significant anticancer effect. Therefore, the synthesised nanocomposite under optimal conditions can be used in the design of new anticancer drugs. © 2019 Amin Golshah, Mojtaba Taran, Mohsen Safaei, Hamid Reza Mozaffari, Mohammad Moslem Imani, Roohollah Sharifi, Hedaiat Moradpoor, Prabhat Upadhyay.
In vitro evaluation of anticancer activity of sodium hyaluronate-titanium dioxide bionanocomposite
(Sciendo, 2019) Mohsen Safaei; Mojtaba Taran; Mohammad Moslem Imani; Hedaiat Moradpoor; Amin Golshah; Prabhat Upadhyay
The purpose of the current research is to optimize the synthesis of sodium hyaluronatetitanium dioxide nanocomposite with the highest anticancer activity. To this end, the Taguchi method was followed to design nine experiments with different ratios of sodium hyaluronate biopolymer, titanium dioxide nanoparticles and stirring times. The results of scanning electron microscopy (SEM) confirmed the synthesis of the nanoparticle and nanocomposite. The comparison of anticancer activity level of synthesized nanocomposites using MTT assay showed that the nanocomposite synthesized in the conditions of experiment 9 (8 mg/ml of titanium dioxide nanoparticles, 2 mg/ml of sodium hyaluronate biopolymer and 60 min stirring time) had the maximum anticancer activity against Michigan Cancer Foundation-7 (MCF-7) cell line. According to the results, the Taguchi method can be employed as an effective and useful strategy to save time and cost in order to determine the optimal conditions for the synthesis of sodium hyaluronate-titanium dioxide nanocomposite with the most favorable anticancer activity. © 2019 Mohsen Safaei et al., published by Sciendo.