Browsing by Author "Amit Kumar Dubey"

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In silico characterization of pectate lyase protein sequences from different source organisms
(2010) Amit Kumar Dubey; Sangeeta Yadav; Manish Kumar; Vinay Kumar Singh; Bijaya Ketan Sarangi; Dinesh Yadav
A total of 121 protein sequences of pectate lyases were subjected to homology search, multiple sequence alignment, phylogenetic tree construction, and motif analysis. The phylogenetic tree constructed revealed different clusters based on different source organisms representing bacterial, fungal, plant, and nematode pectate lyases. The multiple accessions of bacterial, fungal, nematode, and plant pectate lyase protein sequences were placed closely revealing a sequence level similarity. The multiple sequence alignment of these pectate lyase protein sequences from different source organisms showed conserved regions at different stretches with maximum homology from amino acid residues 439-467, 715-816, and 829-910 which could be used for designing degenerate primers or probes specific for pectate lyases. The motif analysis revealed a conserved Pec-Lyase-C domain uniformly observed in all pectate lyases irrespective of variable sources suggesting its possible role in structural and enzymatic functions. © 2010 Amit Kumar Dubey et al.