Browsing by Author "Amit Kumar Sharma"

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Antioxidant capacity and radical scavenging effect of polyphenol rich Mallotus philippenensis fruit extract on human erythrocytes: An in vitro study
(Hindawi Publishing Corporation, 2014) Mayank Gangwar; Manish Kumar Gautam; Amit Kumar Sharma; Yamini B. Tripathi; R.K. Goel; Gopal Nath
Mallotus philippinensis is an important source of molecules with strong antioxidant activity widely used medicinal plant. Previous studies have highlighted their anticestodal, antibacterial, wound healing activities, and so forth. So, present investigation was designed to evaluate the total antioxidant activity and radical scavenging effect of 50% ethanol fruit glandular hair extract (MPE) and its role on Human Erythrocytes. MPE was tested for phytochemical test followed by its HPLC analysis. Standard antioxidant assays like DPPH, ABTS, hydroxyl, superoxide radical, nitric oxide, and lipid peroxidation assay were determined along with total phenolic and flavonoids content. Results showed that MPE contains the presence of various phytochemicals, with high total phenolic and flavonoid content. HPLC analysis showed the presence of rottlerin, a polyphenolic compound in a very rich quantity. MPE exhibits significant strong scavenging activity on DPPH and ABTS assay. Reducing power showed dose dependent increase in concentration absorption compared to standard, Quercetin. Superoxide, hydroxyl radical, lipid peroxidation, nitric oxide assay showed a comparable scavenging activity compared to its standard. Our finding further provides evidence that Mallotus fruit extract is a potential natural source of antioxidants which have a protective role on human Erythrocytes exhibiting minimum hemolytic activity and this justified its uses in folklore medicines. © 2014 Mayank Gangwar et al.
Comparative analysis of diversity based on morpho-agronomic traits and microsatellite markers in common bean
(2009) Vipin Kumar; Shailendra Sharma; Amit Kumar Sharma; Shiveta Sharma; K. Venkataramana Bhat
Morpho-agronomic traits and microsatellite markers were used to survey genetic diversity in 115 common bean genotypes that included 70 Indian landraces, 24 released varieties and 21 exotic accessions. Twelve morpho-agronomic traits, namely, days to 50% flowering, leaflet length, leaflet width, pod length, pod width, number of pods per plant, days to maturity, seed length, seed width, number of seeds per pod, 100 seed weight and seed yield per plant were studied. Field data of two consecutive years were subjected to multivariate analysis as proposed by Mahalanobis's D2-statistics, Tochers method of clustering and combined analysis of variance. Seventeen microsatellite markers were also used to examine genetic diversity at molecular level that showed polymorphic information content (PIC) in the range of 0.00-0.684. Dendrograms based on Euclidean distances and UPGMA analysis showed the presence of majority of released varieties into single cluster, which pointed toward their low genetic base in comparison to indigenous landraces and exotic germplasm. Significant correlation existed between morphological genetic distance and microsatellite genetic distance tested by Mantel test (r = 0.876). © Springer Science+Business Media B.V. 2009.
Comparative analysis of phenolic and flavonoid content of Jatropha Curcas Linn.
(2012) Amit Kumar Sharma; Mayank Gangwar; Adya Prasad Chaturvedi; A.S.K. Sinha; Yamini B. Tripathi
Jatropha species belong to the family Euphorbiaceae commonly called physic nut, purging nut or pig nut. Based on their economic and pharmaceutical importance, we investigated the total phenolic and flavonoid content of different parts of whole plant. The dried plant powder was subjected to Soxhlet extraction with methanol. Phenolic content was estimated using Folin ciocalteau reagent, flavonoid using aluminium chloride (2%) reagent as quercetin equivalent and TLC analysis using chloroform, benzene, hexane and ethyl acetate for the analysis of number of constituent in different extract. Methanolic extract of J. curcas leaf contain higher phenolic content (38.8±2.14) followed by latex (29.12±4.52) and root (26.15±3.84). Flavonoid contents was found to be maximum in cake (26.15±3.84) followed by latex (18.14±2.54). TLC analysis shows four to six spots in different extract showing varying number of components using hexane, chloroform and benzene solvent. The study revealed that the extracts of J. curcas showed presence of high amount of phenolic and flavonoid compounds especially presscake along with secondary metabolites suggesting its use for treatment of various infections.
Comparative in vitro antimicrobial and phytochemical evaluation of methanolic extract of root, stem and leaf of Jatropha curcas linn
(Pharmacognosy Network Worldwide, 2012) Amit Kumar Sharma; Mayank Gangwar; Ragini Tilak; Gopal Nath; Akhoury Sudhir Kumar Sinha; Yamini Bhusan Tripathi; Dharmendra Kumar
Background: Earlier researchers have reported antibacterial activity of different specific parts, but none of the reports show the comparative microbial and phytochemical studies of root, stem and leaf extract. Objective: To compare and investigate antimicrobial, qualitative phytochemical studies, phenol, flavonoid and TLC analysis of root, stem bark, leaf extracts of Jatropha curcas Linn family Euphorbiaceae. Methods: The dried plant powder was subjected to Soxhlet extraction with methanol. These solvent extracts were subjected to a preliminary phytochemical screening to detect the different chemical principles present viz., carbohydrates, proteins, amino acids, steroids, glycosides, alkaloids, flavonoids, tannins and phenolic compounds, fixed oils. Antimicrobial activity was evaluated by disc diffusion method and minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), minimum fungicidal concentration (MFC) was calculated by micro dilution method. Thin layer chromatography was also performed using solvent system chloroform, benzene, hexane, and ethyl acetate for the analysis of a number of constituents in the plant extract. The content of the total phenolics in the extract was determined spectrometrically according to the Folin-Ciocalteu procedure and calculated as catechol equivalent. The content of total flavonoids in the extract was determined and calculated as quercetin equivalent. Result: These extracts showed antibacterial, antifungal activities against gram-positive and gramnegative bacteria with varying magnitudes. The phytochemical analysis showed the presence of alkaloid, saponin, tanins, terpenoids, steroids, glycosides, phenols and flavonoids. Maximum phenolic content (38.8) was found in leaf extract and flavonoid content (18.14) in latex of plant. Discussion: It is concluded that the antimicrobial activity showed by the plant was due to the presence of these phytochemicals. Further studies are highly needed for drug development.
Evaluation of antimicrobial activity and bronchodialator effect of a polyherbal drug-Shrishadi
(Asian Pacific Tropical Biomedicine Press, 2012) Divya Kumari Kajaria; Mayank Gangwar; Dharmendra Kumar; Amit Kumar Sharma; Ragini Tilak; Gopal Nath; Yamini Bhusan Tripathi; J.S. Tripathi; S.K. Tiwari
Objective: To investigate antimicrobial and bronchodialator effect of hydroalcholic extract of polyherbal drug Shirishadi containing Shirisha (Albezzia lebbeck), Nagarmotha (Cyprus rotandus) & Kantakari (Solanum xanthocarpum). Methods: Antimicrobial activity was evaluated by disc diffusion method and MIC, MBC, MFC were calculated by micro dilution method. Hydroalcholic extract of this preparation was investigated for its phytochemical analysis, phenol and flavonoid were determined by spectrophotometric method and in vivo bronchodilator effect was analysed by convulsion time. Results: The phytochemical tests revealed presence of alkaloids, anthraquinones, carbohydrates, flavonoids, saponins and tannins. The antimicrobial result showed the MIC of 6.25 mg/mL against Staphylococcus aureus and 12.5 mg/mL for Escherichia coli and 12.5 mg/mL against remaining bacteria tested, with strong antifungal activity. The maximum inhibition zone is found against Pseudomonas aeruginosa with MIC 16 mg/mL. Drug showed significant bronchodilator effect with 27.86% & 36.13% increase in preconvulsion time of guinea pigs pretreated with 100 & 200 mg/kg body weight of extract. Conclusions: The study reveals that the extracts possess antibacterial activity and antifungal activity in a dose dependent manner. This antimicrobial property may be due to presence of several saponins, further studies are highly needed for the drug development. © 2012 Asian Pacific Tropical Biomedical Magazine.
Genetic diversity in Indian common bean (Phaseolus vulgaris L.) using random amplified polymorphic DNA markers
(2008) Vipin Kumar; Shailendra Sharma; Amit Kumar Sharma; Mukesh Kumar; Shiveta Sharma; Sunil Malik; K.P. Singh; R.S. Sanger; K.V. Bhat
Genetic diversity of twenty-six common bean (Phaseolus vulgaris L.) accessions of diverse geographical origin was studied using Random Amplified Polymorphic DNA (RAPD) markers. Fifteen out of forty four primers screened showed polymorphism across present set of genotypes. A total of 124 amplicons were scored using these 15 primers. Ninety five percent of the amplified products showed polymorphism, indicating fair amount of variation at the DNA level among these accessions. Cluster analysis delineated the genotypes in to four groups. © 2008 Prof. H.S. Srivastava Foundation for Science and Society.
India's shift toward sustainable energy: A comprehensive approach to renewable energy integration and environmental sustainability
(American Institute of Physics, 2024) Surajit Mondal; Arnab Mondal; Jaswant Rathore; Kota Krishna Koundinya; Amit Kumar Sharma
India's reliance on coal for energy has led to pollution, carbon emissions, and environmental hazards. To combat these issues, the Indian government is promoting renewable energy to enhance energy efficiency and reduce environmental impacts. By investing in solar, hydro, and wind power, India aims to minimize pollution and greenhouse gas emissions while meeting energy demands sustainably. The shift to renewable energy not only benefits the environment but also reduces energy import dependency and lowers costs. Renewable energy technologies like solar, hydro, and wind play a crucial role in power generation, offering clean alternatives derived from natural resources. The government's focus on providing 24/7 affordable electricity for all has spurred innovations in off-grid electricity generation, particularly in sun-rich regions. These advancements have attracted private sector investments, improved energy efficiency, and addressing energy shortages. India's target to install 337 GW of renewable energy capacity by 2027, with significant contributions from foreign and private sectors, underscores the country's vast renewable energy potential. Initiatives like the Electricity Act 2001 and rural electrification have enhanced energy conservation and distribution efficiency. The transition to electric vehicles and advancements in renewable energy technologies signal a promising future for India's energy sector, with opportunities for innovation, employment growth, and sustainable development. This article discusses the government's initiatives to promote renewable energy, the role of solar, hydro, and wind power in power generation, and the potential benefits of this transition for the Indian economy and environment. © 2024 Author(s).