Browsing by Author "Amit Verma"

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Development of colloidal Gold nanoparticle based lateral-flow assay for rapid detection of SARS-CoV-2 showing enhanced sensitivity and specificity
(Open Science Publishers LLP Inc., 2023) Suresh Singh; Tapan Singha; Rajat Maheshwari; Pankaj Yadav; Arun Kumar; Harendra Bhoj; Bharti Sharma; Amit Verma; Amit Gupta; Harsh Singh; Sonal Mishra; Rajeshwar Sinha
To stop the spread of COVID-19 in this outbreak, diagnostic testing is essential. Quick diagnostic tests must be employed in this pandemic, which is brought on by the severe acute respiratory syndrome (SARS)-CoV-2 virus, to successfully treat and manage COVID-19. There are several problems with the present RT-PCR system that the lateral flow assay (LFA), a kind of clinically sensitive diagnostic test, may be able to fix, especially in low-and middle-income nations. Gold nanoparticle-(AuNP-LFA) is a practical method for detecting COVID-19 in basic hospitals and laboratories, particularly in emergency situations where many samples must be quickly examined. Safe, accurate, and non-toxic diagnostic tests must be employed during the pandemic, to successfully treat and manage COVID-19. Recombinant SARS-CoV-2 nucleocapsid monoclonal antibody was employed to detect COVID-19 antigens in the presence of patients to establish a fast LFA for COVID-19. Synthesis of colloidal gold particles and antibody colloidal gold conjugates was evaluated by using UV/Vis spectroscopy. A capture line made of anti-SARS-CoV-2 antibody was coated on nitrocellulose membrane. To create the control line, goat anti-mouse IgG monoclonal antibody was coated. On a polystyrene backing board, the immunochromatographic strip was constructed in the ideal order. Using ELISA as the standard procedure, the strips’ sensitivity and specificity were assessed. The results’ stability and repeatability were evaluated over a 9-month period. Colloidal gold nanoparticle-based LFAs created in this study can be employed for quicker and more accurate detection of SARS-CoV-2. © 2023 Singh, et al.
Diagnostic Approaches for Covid-19: Present Status and Future Prospects
(AMG Transcend Association, 2023) Rajat Maheshwari; Tapan K. Singha; Preeti Vashisth; Amit Verma; Amit Gupta; Suresh C. Singh; Sonal Mishra; Varsha K. Singh; Rajeshwar P. Sinha
During this COVID-19 outbreak, diagnostic tests were crucial in controlling its spread. This pandemic, which was caused by the SARS-CoV-2 virus, has created an urgent need for rapid diagnostic testing to enable efficient treatment and control of COVID-19. Many institutes and companies are trying to develop effective methods for detecting COVID-19. The essential indicative instrument presently utilized is reverse transcription-polymerase chain response (RT-PCR), which can have great affectability. Tragically, execution costs, time is taken, and false-negative results have limited the use of RT-PCR. Lateral flow assays (LFAs) are a type of diagnostic test that is clinically sensitive enough, might bridge various inadequacies in the present RT-PCR system, especially in low-and middle-income countries. In basic hospitals and laboratories, AuNP-LFA is a viable technique for diagnosing COVID-19, especially in emergency settings where a large number of samples must be examined quickly. This review summarizes the different diagnostic approaches for detecting the SARS-COV-2. This review thus provides guidelines for the scientists and technicians engaged in detecting SARS-COV-2. An accurate and sensitive detection system would enormously benefit in controlling this pandemic. © 2022 by the authors.
Evaluation of canine bone marrow-derived mesenchymal stem cells for experimental full-thickness cutaneous wounds in a diabetic rat model
(Taylor and Francis Ltd., 2021) Deepika Bist; A.M. Pawde; Amarpal; Prakash Kinjavdekar; Reena Mukherjee; K.P. Singh; Med Ram Verma; Khan Sharun; Amit Kumar; Pawan K. Dubey; Divya Mohan; Amit Verma; G. Taru Sharma
Background: The wound healing potential of canine bone marrow-derived mesenchymal stem cells (BMSCs) was evaluated in the excisional wound of streptozotocin-induced diabetic rats. Research design and methods: Xenogenic BMSCs were collected aseptically from the iliac crest of healthy canine donors under general anesthesia. Full-thickness experimental wounds (20 × 20 mm2) on the dorsum of forty-eight adult healthy Wistar white rats. The wounds were assigned randomly to three treatment groups: PBS (Group A) or BMSCs (Group B) injected into the wound margins on days 0, 7, and 14 or BMSCs (Group C) injected into the wound margins on days 7, 14, and 21 post-wounding. The degree of wound healing was evaluated based on macroscopical, hemato-biochemical, histopathological, and histochemical parameters. Results: The results indicated granulation tissue formation with reduced exudation and peripheral swelling in the treatment groups compared to the control group A. Similarly, the degree of wound contraction was significantly higher in groups B and C animals than group A on days 14 and 21 post-wounding. The transplantation of BMSCs resulted in early drying of wounds, granulation tissue appearance, and enhanced cosmetic appearance. Conclusion: The histopathological, histochemical, and gross findings suggested the therapeutic potential of xenogeneic mesenchymal stem cell therapy in managing diabetic wounds. Abbreviations: BMSCs-bone marrow-derived mesenchymal stem cells, PBS-phosphate-buffered saline, MSCs-mesenchymal stem cells, FBS-fetal bovine serum, ECM-extracellular matrix. © 2021 Informa UK Limited, trading as Taylor & Francis Group.
Phytomicrobiome Interactions and Sustainable Agriculture
(wiley, 2021) Amit Verma; Jitendra Kumar Saini; Abd El-Latif Hesham; Harikesh Bahadur Singh
A guide to the role microbes play in the enhanced production and productivity of agriculture to feed our growing population Phytomicrobiome Interactions and Sustainable Agriculture offers an essential guide to the importance of ‘Phytomicrobiome’ and explores its various components. The authors – noted experts on the topic – explore the key benefits of plant development such as nutrient availability, amelioration of stress and defense to plant disease. Throughout the book, the authors introduce and classify the corresponding Phytomicrobiome components and then present a detailed discussion related to its effect on plant development: controlling factors of this biome, its behaviour under the prevailing climate change condition and beneficial effects. The book covers the newly emerging technical concept of Phytomicrobiome engineering, which is an advanced concept to sustain agricultural productivity in recent climatic scenario. The text is filled with comprehensive, cutting edge data, making it possible to access this ever-growing wealth of information. This important book: Offers a one-stop resource on phytomicrobiome concepts Provides a better understanding of the topic and how it can be employed for understanding plant development Contains a guide to sustaining agriculture using phytomicrobiome engineering Presents information that can lead to enhanced production and productivity to feed our growing population Written for students, researchers and policy makers of plant biology, Phytomicrobiome Interactions and Sustainable Agriculture offers a clear understanding of the importance of microbes in overall plant growth and development. © 2021 John Wiley & Sons Ltd. All rights reserved.
Preface
(wiley, 2021) Amit Verma; Jitendra Kumar Saini; Abd El-Latif Hesham; Harikesh Bahadur Singh
[No abstract available]
Unethical issues in advertising
(Associated Management Consultants Pvt. Ltd., 2011) Amit Verma
[No abstract available]