Browsing by Author "Anirbana Parida"

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Cyanobacteria: a key player in nutrient cycling
(Elsevier, 2023) Alka Bhardwaj; Prashansa Singh; Neha Gupta; Samujjal Bhattacharjee; Ankit Srivastava; Anirbana Parida; Arun Kumar Mishra
Cyanobacteria are photosynthetic prokaryotic organisms that are found in various aquatic and terrestrial environments. They are one of the oldest and most primitive forms of life on Earth, playing critical role in the biological nutrient cycling of different habitats. The phenomenon of nutrient cycling delineates the continual recycling of essential elements, which are rendered accessible to the biota of an ecosystem. Cyanobacteria possess the ability to fixate atmospheric nitrogen and transform it into a bioavailable form, which can be utilized by other organisms in the ecosystem. This process is called nitrogen fixation, and it helps to increase the availability of nitrogen in the ecosystem, which is an essential nutrient for the growth of different life forms. They also play a key role in carbon cycling by capturing carbon dioxide through photosynthesis and releasing oxygen into the atmosphere. Indeed, cyanobacteria played a significant role in making the early environment aerobic by producing oxygen through photosynthesis. This process helps to regulate the amount of carbon dioxide in the atmosphere, which is important for mitigating the effects of climate change. Additionally, cyanobacteria can contribute to the cycling of other nutrients, such as phosphorus and sulfur, by releasing them from organic matter and making them available for other organisms to use. Overall, cyanobacteria are crucial for the cycling of different nutrients, including nitrogen, carbon, phosphorus, and sulfur, and their impact on the health of the ecosystem cannot be overstated. The presence of these microorganisms is essential for ensuring a stable and thriving environment, and their involvement in nutrient cycling and oxygenic photosynthesis has constituted a critical component in the evolutionary history of life on Earth. © 2024 Elsevier Inc. All rights reserved.
Efficient biodegradation of sodium dodecyl sulfate (SDS) by the cyanobacterium Fischerella sp. lmga1 harbouring SdsA1 hydrolase
(Springer Science and Business Media B.V., 2023) Samujjal Bhattacharjee; Ankit Srivastava; Anirbana Parida; Neha Gupta; Prashansha Singh; Satya Shila Singh; Arun Kumar Mishra
Sodium dodecyl sulfate (SDS) accumulation in nature impart detrimental effects on various lifeforms as it truncates cellular proteins. The present study exhibited SDS bioremediation ability of cyanobacterium Fischerella sp. lmga1. Phylogeny across cyanobacterial phyla depicted uneven distribution and abundance of SdsA1 hydrolase (characterised for SDS hydrolysis in Pseudomonas aeruginosa PA01) in Microcystis spp. and Fischerella spp. Further, Fischerella sp. lmga1, was administered with 200 µM SDS to validate not only the bioremediation capability but also the physiological adjustments during SDS hydrolysis. The preliminary effect of SDS was harsh, as the cyanobacterial growth was significantly reduced along with subsequent decline in photopigment, chlorophyll a fluorescence and carbohydrate. Nevertheless, drastic rejuvenation upon 8th day of treatment and increment in photosynthetic capacity suggested the resilience of lmga1 against SDS. Further, about 50% removal of SDS with highest degradation rate was observed on 10th day of treatment, followed after substantial increment in the expression of sdsA1 on 8th day. Interestingly, the Fourier-transform infrared (FTIR) depicted homeostasis in the carbon allocation pattern or membrane lipid dynamics during SDS removal, indicating the endurance of lmga1. Although, the peak intensity at 1077 cm−1 reduced on day 4 corresponded to DNA damage, yet increased peak intensity on 8th day ascertained damage repair. Molecular docking of SdsA1 hydrolase of Fischeralla thermalis CCME 5301 with SDS depicted low binding energy of -4.3 kCal mol−1, thereby endorsing stable binding. Thus, this study corroborated the application of lmga1 for removal of surfactant as it displayed commendable resilience and SDS removal capability. Graphical abstract: [Figure not available: see fulltext.] © 2023, The Author(s), under exclusive licence to Springer Nature B.V.
Harnessing SDS as a source of sulfur: a bioremediation strategy of Fischerella sp. lmga1
(Springer Science and Business Media B.V., 2025) Ankit Srivastava; Anirbana Parida; Samujjal Bhattacharjee; Neha K. Gupta; Satya Shila Singh; Arun Kumar Mishra
Sodium dodecyl sulfate (SDS), a widely used anionic surfactant, is a pervasive aquatic pollutant with documented ecotoxicity and persistence in the environment. In this study, we investigated metabolic response of the filamentous, heterocytous cyanobacterium Fischerella sp. lmga1 under sulfur starvation, focusing on its capacity to degrade SDS and utilize it as an alternative sulfur source. Sulfur-deprived cultures supplemented with 150 µM SDS initially exhibited chlorosis and physiological stress, but showed significant recovery by 14 days, including increased growth and better photosynthetic performance. A significant rise in intracellular sulfur content was observed, suggesting active sulfur acquisition. Expression analysis revealed strong induction of genes involved in sulfur uptake and assimilation (e.g., cysT, cysW, sbp, sat), alongside an ~ 880-fold upregulation of sdsA1 on day 10, encoding an SDS hydrolase. Correlation analyses showed that increased sdsA1 expression coincided with improvements in viability and sulfur status. This underscored a coordinated mechanism of SDS degradation and concomitant sulfur assimilation in Fischerella, indicating towards a novel adaptive strategy. Thus, this study establishes Fischerella as a promising candidate for bioremediation of sulfonated pollutants in aquatic systems and expands the knowledge of metabolic plasticity of cyanobacteria. © The Author(s), under exclusive licence to Springer Nature B.V. 2025.
Nitric Oxide: A Double-Edged Sword in Photosynthetic Stress Responses
(Springer Nature, 2024) Neha Gupta; Ankit Srivasatava; Anirbana Parida; Arun Kumar Mishra
Nitric oxide (NO), a small gaseous molecule with redox activityserves as a central regulator of growth, development, immunity. This remarkable signaling molecule orchestrates a complex symphony of biological responses, navigating through intricate pathways that influence essential aspects of plant life. The absence of canonical Nitric Oxide Synthases (NOS) in higher plants has led to the discovery of alternative NO production pathways, featuring key contributors like nitrate reductase (NR) in mitochondria, peroxisomes, and chloroplasts. In the realm of plant physiology, NO emerges as a multifaceted player, significantly impacting reproduction, symbiotic interactions, senescence, and defense mechanisms. In symbiotic relationships, NO plays a pivotal role in interactions with rhizobia, mycorrhizae, and lichens, influencing nodule development and senescence. Furthermore, NO governs plant senescence, acting both as a signaling molecule and a modulator of hormone-induced processes. In defense responses, NO collaborates with reactive oxygen species, influencing hypersensitive cell death. Beyond growth, NO showcases its potential in postharvest applications, preserving sensory attributes, enhancing nutritional quality, and serving as an eco-friendly alternative for pest and disease control in horticultural crops. As research delves deeper, unveiling additional dimensions of NO's contributions, it holds promise for innovative applications in agriculture, environmental management, and sustainable horticulture. © The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Nature Singapore Pte Ltd. 2024.
The hidden world of cyanobacterial cell death: classification, regulatory mechanisms, and ecological significance
(Elsevier, 2023) Samujjal Bhattacharjee; Anirbana Parida; Anabella Aguilera; María Victoria Martin
Cyanobacteria are ancient, globally widespread photosynthetic prokaryotes that synthesize potent toxins and form blooms, a major ecological and human health problem worldwide. Conditions promoting massive bloom proliferation have been extensively studied, but mechanisms causing their abrupt termination are poorly understood. Cell death plays a vital role in the dynamics of ephemeral blooms, determining the flow and fate of organic matter and nutrients. In recent decades, regulated cell death (RCD) induced by biotic or abiotic stresses has become a major mechanism to explain the disappearance of blooms of harmful algal species. However, the molecular basis and physiological mechanisms behind RCD in cyanobacteria are still largely unknown. This chapter aims to describe recent advances in regulated cell death, its nomenclature, implications for cyanobacterial fitness, and ecological relevance. Additionally, we describe methods to study cell demise in this group of photosynthetic organisms. This information contributes to increasing our understanding of how cyanobacteria cope with environmental stress and activate RCD and opens new applications in biotechnology, for instance, the development of new technologies to control harmful blooms and ensuring water quality, and preserve the health of the population. © 2024 Elsevier Inc. All rights reserved.