Browsing by Author "Anju Gautam"

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A new era of Nano!!! Comparative evaluation of ganglioside polymeric nanoparticle coated satranidazole gel and 1% metronidazole gel for the treatment of periodontitis
(Wolters Kluwer Medknow Publications, 2022) Shivam Kesarwani; Sarita Parihar; Sanjay Singh; Anju Gautam; Aishwarya Pandey; Md Anjum
Background: The present study was intended to comparatively assess the efficacy of ganglioside polymeric nanoparticle-coated 0.25% satranidazole-loaded nanoparticles in gel form with that of the commercially available 1% metronidazole gel as a local drug delivery (LDD) agent for the treatment of periodontal pockets. Materials and Methods: A split-mouth randomized clinical trial was carried out in 46 chronic periodontitis patients with probing pocket depth (PPD) ≥4 mm or clinical attachment loss greater than 3 mm on both quadrants of the same arch. Full-mouth scaling and root planing (SRP) was performed for all the patients followed by application of 0.25% satranidazole-loaded nanoparticles in gel form on one site (Group 1) and commercially available 1% metronidazole gel on another site (Group 2). Clinical parameters (gingival index, plaque index, PPD, clinical attachment level gain, and bleeding on probing) and microbiological analysis of the subgingival plaque samples were performed and assessed at baseline, after SRP, 21 st day, and 90 th day post treatment. Unpaired 't'-test and ANOVA tests were used for intergroup and intragroup comparison of recorded parameters. Results: The results showed that the satranidazole-loaded nanoparticle group as an adjunct to SRP in chronic periodontitis showed a statistically significant improvement in all the clinical parameters and a fewer relapse of microbial flora in comparison with the metronidazole group as an LDD agent. Conclusion: The present study depicted that both the LDD agents showed an effective improvement of clinical as well as microbiological parameters, but the satranidazole group consistently produced better results than the metronidazole group and hence has a promising future as an LDD agent in treating periodontal pockets. © 2022 Wolters Kluwer Medknow Publications. All rights reserved.
A ray of hope for the hopeless: Hemisection of mandibular molar with socket preservation
(2012) Pushpendra K. Verma; Ruchi Srivastava; Harak C. Baranwal; Anju Gautam
Introduction: The management, treatment and long-term retention of mandibular molar teeth with furcation involvement have always been a challenge in periodontal therapy. Hemisection has been used successfully to retain teeth with furcation involvement. The term hemisection refers to the sectioning of a molar tooth, with the removal of an unrestorable root which may be affected by periodontal, endodontic, structural (cracked roots), or caries. Case Report: This case report describes a simple procedure of hemisection in a mandibular molar with socket preservation with help of an alloplastic bone graft and subsequent restoration of the tooth with fixed prothesis. Discussion: Hemisection represents a form of conservative procedure, which aims at retaining as much of the original tooth structure as possible. It may be a suitable alternative to extraction. Hemisection of the affected tooth allows the preservation of tooth structure, alveolar bone and cost savings (time and money) over other treatment options. The preservation of posterior abutment teeth permits oral rehabilitation with fixed bridges instead of removable prosthesis. Conclusion: The keys to long term success appear to be thorough diagnosis followed by interdisciplinary approach with endodontic, surgical and prosthetic procedures. Preservation of a hopeless tooth is possible by selecting patients with good oral hygiene, and careful surgical and restorative management.
Clinical evaluation of coronally advanced flap along with autologous platelet-rich fibrin membrane for root coverage of isolated gingival recession
(Nova Science Publishers, Inc., 2020) Anju Gautam; Awadhesh Chandra Nagar; Karuna Lodhi
Aim: The aim of this study was to evaluate the clinical efficacy of autologous platelet rich fibrin membrane (PRF) along with coronally advanced flap (CAF) for root coverage of isolated gingival recession. Materials and Methods: Thirty isolated Miller class I or II gingival recession sites in 28 patients were randomly divided into control (CAF alone) and test (CAF+PRF) groups. The Clinical outcome was determined by clinical parameters such as gingival recession depth (GRD), probing pocket depth (PPD), clinical attachment level (CAL), width of keratinized gingiva (WKG), width of attached gingiva (WAG) and gingival thickness (GTH) at baseline, 1st, 3rd, and 6th month postoperatively. Results: Results from the present study indicated that both the groups, CAF+PRF and CAF demonstrated an overall significant improvement in all the clinical parameters assessed. However the result of this study found no statistically significant difference between the two groups except gain in gingival thickness. Conclusion: CAF alone is a predictable treatment for isolated gingival recession defects. The addition of PRF membrane with CAF provides better outcome in terms of root coverage, gain in CAL and gingival thickness (GTH). © 2020 Nova Science Publishers, Inc.
Comparative evaluation of mucin and total protein in periodontal disease before and after nonsurgical periodontal therapy
(Wolters Kluwer Medknow Publications, 2024) J.P. Vishnu; Anju Gautam; Surendra Pratap Mishra; Farhan Durrani; Fouzia Imran; Ekta Kumari
Background: Periodontal ailments cause a quantum leap in the biomarker profile of the saliva. This profile is, in fact, the epiphany of the scale and extent of the disease. Both gingivitis and periodontitis are chronic inflammatory diseases with a step-grade progression. The study aimed to determine the response of the host in these conditions by analyzing concentrations of salivary mucin and total protein activity, before and after nonsurgical periodontal therapy (NSPT). Materials and Methods: Sixty adult subjects were clinically examined and divided into three groups (n = 20) according to the clinical assessment and categorized as Group I (healthy), Group II (gingivitis), and Group III (chronic periodontitis). Whole saliva was collected, and salivary mucin and total protein levels were quantitatively measured at baseline in all the groups and additionally after NSPT in Groups II and III. Results: Levels of mucin and total protein increased in patients with gingivitis and periodontitis. There was a slight decline in mucin levels in periodontitis patients in comparison with the gingivitis group. A positive correlation was found between the respective clinical parameters of both the groups along with their levels of salivary mucin and total protein. It indicated that the response of salivary glands to increase their protective potential caused the change among the groups. Conclusion: Periodontal diseases induce an increase in the levels of mucins and proteins, which is believed as the action of the salivary glands to protect the oral cavity and put off the chaos caused by the microorganisms. © 2024 Indian Society of Periodontology | Published by Wolters Kluwer - Medknow.
Correlation of ABO blood group phenotype and rhesus factor with periodontal disease: An observational study
(Medknow Publications, 2017) Anju Gautam; Neelam Mittal; T.B. Singh; Ruchi Srivastava; Pushpendra K. Verma
Background: The knowledge of the ABO blood group phenotype of the patients and their correlation with the periodontal disease maybe important in the development of early treatment strategies, and it would be helpful to target non-responding areas to periodontal therapy of the susceptible individuals. Aims: The present study was conducted to determine whether there was any correlation between periodontal diseases and ABO blood groups and Rh factor. Material and Method: This study was carried out on 537 subjects attending Faculty of Dental Sciences OPD in BHU. Subjects were divided into three groups: group I (healthy subjects), group II (subjects with gingivitis), and group III (subjects with periodontitis) based on periodontal examination (Gingival index, Bleeding Index, Probing pocket depth and clinical attachment level). ABO Blood grouping were done and correlated with the periodontal status of study subjects. Statistical Analysis: Data was analyzed using the statistical software namely Statistical Package for the Social Sciences (SPSS, Version 16, IBM Analytics) and Systat 8.0. Results: In this study, there was a greater prevalence of gingivitis in blood group O and periodontitis in blood group B. The blood group AB showed the least prevalence of periodontal diseases. Similarly gingivitis and peridontitis were significantly higher among Rhesus positive groups when compared with Rhesus negative groups. Conclusion: Considering the results of this study, it can be concluded that ABO blood groups and Rh factor could be a risk factor for the development of periodontal disease. © 2017 Contemporary Clinical Dentistry.
Design and characterization of anti-microbial novel herbal nanofiber scaffolds for the management of periodontal diseases
(Nature Research, 2025) Pragati Dubey; Neelam Mittal; Brahmeshwar Mishra; S. Manjit; Pooja Goswami; Biplob Koch; Anju Gautam; Pradeep Harish Kumar; Kavindra Nath Tiwari
Design and evaluation of PCL and gelatin loaded nanofiber containing herbal formulation named Ashvakatri in the treatment of chronic periodontitis. The electro-spinning method was used to create the composite nano-fibers containing herbal formulation, which were then evaluated for various parameters including in-vitro antimicrobial study and in-vitro drug release. An interconnected, continuous and bead free nanofiber with an average diameter of 150–250 nm was exhibited by the optimized nanofiber. The addition of gelatin and PCL to the optimized nanofiber improved its biocompatibility and bioadhesive characteristics. According to anti-microbial investigation and CLSM study indicated the remarkable inhibition of periodontal pathogens such as Aggregabacter actinomycetemcomitans (AI), Porphyromonas Gingivilis (PG), Fusobacterium spp (FB), and Porphyromonas intermedia (PI) respectively by optimized nanofiber and in-vitro drug release study also demonstrated the controlled release of composite nanofiber for over 220 h. Additionally, the MTT assay and in-vitro scratch assay indicated the composite NF had no cytotoxicity to the fibroblast cell line. The fabricated NF is a potential option for chronic periodontitis treatment since it expedited anti-bacterial, cytotoxic and sustained release medications. © The Author(s) 2025.
Erratum to: Pyogenic Granuloma - Hyperplastic Lesion of the Gingiva: Case Reports (The Open Dentistry Journal, (2012), 6, (153-156))
(2012) Pushpendra Kumar Verma; Ruchi Srivastava; H.C. Baranwal; T.P. Chaturvedi; Anju Gautam; Amit Singh
Pyogenic granuloma is a reactive hyperplasia of connective tissue in response to local irritants. It is a tumourlike growth of the oral cavity, frequently located surrounding the anterior teeth or skin that is considered to be neoplastic in nature. It usually arises in response to various stimuli such as low-grade local irritation, traumatic injury, hormonal factors, or certain kinds of drugs. Histologically, the surface epithelium may be intact, or may show foci of ulcerations or even exhibiting hyperkeratosis. It overlies a mass of dense connective tissue composed of significant amounts of mature collagen. Gingiva is the most common site affected followed by buccal mucosa, tongue and lips. Pyogenic granuloma in general, does not occur when excised along with the base and its causative factors. This paper presents some cases of a pyogenic granuloma managed by surgical intervention.
Evaluation of nanofiber scaffolds laden Ashvakatri in the management of chronic periodontitis-a randomized, controlled split pocket study
(Elsevier B.V., 2025) Pragati Dubey; Neelam Mittal; Brahmeshwar Mishra; Anju Gautam; Naveen P.G. Kumar; M. Rashika
Background: The main objective of periodontal treatment is to stop the progression of periodontal disease. Controlled-release drugs yield promising outcomes when conventional treatment is proven to be insufficient in establishing periodontal health in chronic periodontitis. A low-dose controlled-release delivery method for the treatment of periodontal infection was attempted to be developed in this study. With effective electrospinning, a novel sustained-release medication system including polycaprolactone (PCL) nanofibers containing Ashvakatri (A2) and Tetracycline (TET) was accessed clinically for periodontitis. Method & materials: The electrospinning technique prepared nanofibers with A2 and TET in PCL and gelatin. The A2-loaded nanofiber followed the Higuchi model release and had a sustained impact of 9 days (220 h). 75 periodontal sites from 31 patients with chronic periodontitis (≥5 mm probing depth) followed by 3 groups: Group I received Scaling and root planning (SRP) and blank polymer mat whereas Group II treated with SRP and PCL-GE-A2 nanofiber scaffold/mat, and Group III received SRP + PCL-GE-TET (tetracycline) nanofiber scaffolds. Clinical evaluations of GI, PI, PPD, and CAL were performed on each patient group. Conclusion: Compared to the placebo and standard group, the test group was remarkably associated with improved GI, PI, PPD, and CAL at the end of the study. Therefore, drug-loaded nanofiber was found to be efficacious in treating periodontal diseases and may be appropriate as an alternative treatment. Clinical significance: The fabricated PCL-GE-A2 nanofiber mat was more cost-efficient, minimized the dosage amount, and dosage frequency, and showed no adverse effects or discomforts with increased patient compliance. © 2025 The Authors
Evaluation of the effect of nonsurgical periodontal therapy on malondialdehyde and 8-hydroxy deoxyguanosine levels in chronic periodontitis
(Wolters Kluwer Medknow Publications, 2022) Anju Gautam; Neelam Mittal; Surendra Pratap Mishra; Tej Bali Singh; Akhilesh Chandra
Background: Reactive oxygen species released on stimulation by periodontal pathogens cause oxidation of biomolecules and play significant role in periodontal disease pathogenesis. Aim: This study aimed to evaluate the levels of oxidative by-products malondialdehyde (MDA) and 8-hydroxy deoxyguanosine (8-OHdG) as biomarkers in chronic periodontitis patients compared to healthy as well as before and after nonsurgical periodontal therapy. The correlation between biomarkers and clinical attachment level was also evaluated. Settings and Design: A total of 112 subjects were included in this study. The subjects were divided into two groups (Group I included 56 healthy subjects and Group II constituted 56 chronic periodontitis patients) on the basis of clinical periodontal parameters. Materials and Methods: Group I subjects received no treatment and were evaluated once only for clinical and oxidative stress biomarker parameters. Nonsurgical periodontal therapy was carried out in Group II patients and they were evaluated at baseline and 3 months after therapy. Results: Both salivary and serum levels of MDA and 8-OHdG were found to be increased in chronic periodontitis patients as compared to healthy subjects. After nonsurgical periodontal therapy, the levels of MDA and 8-OHdG significantly reduced. Linear correlation between clinical attachment level and oxidative stress parameters was found to be positive and highly significant. Conclusion: It can be concluded that periodontal therapy is effective in improving the oxidative stress condition. © 2022 Wolters Kluwer Medknow Publications. All rights reserved.
Periosteum as a barrier membrane in the treatment of intrabony defect: A new technique
(Medknow Publications, 2014) Charanjeet Singh Saimbi; Anju Gautam; Mohd. Akhlak Khan; Nandlal
Objective: The purpose of the study was to evaluate the clinical effectiveness of periosteum as a barrier membrane for the treatment of intrabony defects. Materials and Methods: The study was conducted in patients having bilateral intrabony defects. A total of 20 intrabony defects in 10 patients were treated, out of which 10 defects received periosteal barrier and the other 10 defects received conventional open flap debridement procedure. The efficacy of the treatment was assessed using clinical parameters and dentascan. Results: Statistically significant gain in clinical attachment level (CAL) was found in the test group (2.00 ± 0.26 mm) as compared to the control group (0.60 ± 0.22 mm). In both the treatment modalities (test and control groups), a significant decrease in probing pocket depth of 3.90 ± 0.35 mm and 2.90 ± 0.31 mm was observed, respectively. The difference between the two groups was not statistically significant. Bone defect fill was 1.40 ± 0.16 mm for the test group and 0.90 ± 0.18 mm for the control group, but the difference observed was not statistically significant. Conclusion: The results of this study show that periosteal barrier membrane can be a better alternative of barrier membranes for the treatment of intrabony defects.
'Pyogenic granuloma - hyperplastic lesion of the gingiva: Case reports'
(2012) Pushpendra Kumar Verma; Ruchi Srivastava; H.C. Baranwal; T.P. Chaturvedi; Anju Gautam; Amit Singh
Pyogenic granuloma is a reactive hyperplasia of connective tissue in response to local irritants. It is a tumourlike growth of the oral cavity, frequently located surrounding the anterior teeth or skin that is considered to be neoplastic in nature. It usually arises in response to various stimuli such as low-grade local irritation, traumatic injury, hormonal factors, or certain kinds of drugs. Histologically, the surface epithelium may be intact, or may show foci of ulcerations or even exhibiting hyperkeratosis. It overlies a mass of dense connective tissue composed of significant amounts of mature collagen. Gingiva is the most common site affected followed by buccal mucosa, tongue and lips. Pyogenic granuloma in general, does not occur when excised along with the base and its causative factors. This paper presents some cases of a pyogenic granuloma managed by surgical intervention. © Anastasios K. Markopoulos; Licensee Bentham Open.
Unveiling the Lipid Paradox in Oral Leukoplakia: Metabolic Signatures of Dysplasia and Disease Progression
(Springer, 2025) Akhilesh Chandra; R. Keerthika; Rahul Agrawal; Anju Gautam; Richik Tripathi; S. Sandhiya
Oral Leukoplakia (OLK) is the most prevalent oral potentially malignant disorder with an increased risk of malignant transformation. Altered lipid metabolism, often driven by tobacco-induced oxidative stress, may contribute to oral carcinogenesis. However, the relationship between serum lipid profiles and OLK remains ambiguous. This study aimed to evaluate lipid profiles across OLK subtypes and their association with dysplasia and tobacco use to their assess diagnostic and prognostic potential. This cross-sectional prospective study included 114 participants, 57 clinically and histologically confirmed OLK cases and 57 healthy controls. OLK cases were categorized into homogeneous OLK, erythroleukoplakia, verrucous OLK, and OLK with oral submucous fibrosis (OSMF) and their dysplastic status was also evaluated. Serum lipid parameters (TC, TG, HDL, LDL, VLDL) were measured. Statistical comparisons were performed using Mann–Whitney U and Kruskal–Wallis tests (p < 0.05). Although OLK patients showed higher mean lipid levels than controls, the differences were not statistically significant (p > 0.05). Among OLK subtypes, significantly elevated levels of TC, TG, LDL, and VLDL were found in homogeneous OLK, while the lowest levels were observed in erythroleukoplakia (p < 0.00). Lipid levels were significantly lower in dysplastic lesions (p < 0.001). Patients with tobacco use > 5 years had higher TC and LDL levels (p < 0.05), whereas habit frequency showed no significant association. Serum lipid alterations, particularly reduced TC, TG, LDL, and VLDL in dysplastic and high-risk OLK variants, may serve as non-invasive prognostic biomarkers for early risk assessment. © Association of Otolaryngologists of India 2025.