Browsing by Author "Anuradha Singh"

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Allelic sequence variation in the Sub1A, Sub1B and Sub1C genes among diverse rice cultivars and its association with submergence tolerance
(Nature Research, 2020) Anuradha Singh; Yashi Singh; Ajay K. Mahato; Pawan K. Jayaswal; Sangeeta Singh; Renu Singh; Neera Yadav; A.K. Singh; P.K. Singh; Rakesh Singh; Rajesh Kumar; Endang M. Septiningsih; H.S. Balyan; Nagendra K. Singh; Vandna Rai
Erratic rainfall leading to flash flooding causes huge yield losses in lowland rice. The traditional varieties and landraces of rice possess variable levels of tolerance to submergence stress, but gene discovery and utilization of these resources has been limited to the Sub1A-1 allele from variety FR13A. Therefore, we analysed the allelic sequence variation in three Sub1 genes in a panel of 179 rice genotypes and its association with submergence tolerance. Population structure and diversity analysis based on a 36-plex genome wide genic-SNP assay grouped these genotypes into two major categories representing Indica and Japonica cultivar groups with further sub-groupings into Indica, Aus, Deepwater and Aromatic-Japonica cultivars. Targetted re-sequencing of the Sub1A, Sub1B and Sub1C genes identfied 7, 7 and 38 SNPs making 8, 9 and 67 SNP haplotypes, respectively. Haplotype networks and phylogenic analysis revealed evolution of Sub1B and Sub1A genes by tandem duplication and divergence of the ancestral Sub1C gene in that order. The alleles of Sub1 genes in tolerant reference variety FR13A seem to have evolved most recently. However, no consistent association could be found between the Sub1 allelic variation and submergence tolerance probably due to low minor allele frequencies and presence of exceptions to the known Sub1A-1 association in the genotype panel. We identified 18 cultivars with non-Sub1A-1 source of submergence tolerance which after further mapping and validation in bi-parental populations will be useful for development of superior flood tolerant rice cultivars. © 2020, The Author(s).
Alleviation of soil salinity by zinc fertilizer in wheat (Triticum aestivum L.)
(2013) Dhananjaya Singh; P.C. Ram; Anuradha Singh; S.R. Dar; J.P. Srivastava
A study was carried to alleviate adverse effects of soil salinity on physiological parameters of wheat by applying zinc fertilizer. Wheat varieties KRL 19, NW 1012 (salt-tolerant) and HD 2285 (salt-susceptible) were grown in pots under nonsaline (0, 20 kg Zn) and saline (8.0 dSm-1, 20 kg Zn + 8.0 dSm-1) conditions. Observations were recorded for the relative water content (RWC), transpiration rate (E), diffusive resistance, protein, carbohydrate and starch content at both tillering and ear emergence stage. Study revealed that soil salinity adversely affected the relative water content (RWC), transpiration rate, protein, carbohydrate and starch content but diffusive resistance increased significantly in all the three wheat varieties at both stage. Maximum reduction in these parameters was recorded in the susceptible variety (HD 2285), whereas minimum reduction was observed in the tolerant varieties (KRL 19 and NW 1012). Zn application @ 20 kg ha-1 partially alleviated the adverse effects of salinity on protein, carbohydrate and starch content particularly in susceptible variety HD 2285 than salt tolerant varieties (KRL 19 and NW 1012) as compared to no zinc application.
Bio-inspired nanomaterials in agriculture and food: Current status, foreseen applications and challenges
(Academic Press, 2018) Niladri Chaudhry; Sourabh Dwivedi; Vasvi Chaudhry; Anuradha Singh; Quaiser Saquib; Ameer Azam; Javed Musarrat
Nanotechnology is a potential area that revolutionizes almost every sector of life and is predicted to become a major economic force in the near future. Recently, nanomaterials have received great attention for their properties at nanoscale regime and their applications in many areas primarily, agriculture and food sectors. The Nanomaterials are dispersed or solid particles, with a size range of 1–100 nm. In recent times, there has been an increased research work in this area to synthesize nanomaterials using various approaches. The use of natural biomolecules using ‘green’ approach play key role in the synthesis of nanomaterials having different shapes and sizes. Further this ‘green synthesis’ approach not only minimize the cost but also limit the need of hazardous chemicals and stimulates synthesis of greener, safe and environmentally friendly nanoparticles. The present review focus on studies based on the biosynthesis of nanoparticles using biomolecules such as plants, bacteria, fungi, etc. The text summarizes the recent work done globally by renowned researchers in area of biosynthesis of nanomaterials. It also discusses the potential applications of biologically mediated nanomaterials in the areas of agriculture and food and a critical evaluation of challenges within this field. © 2018 Elsevier Ltd
Buddhism in Sarnath: An account of two Chinese travellers
(ACCB Publishing, 2014) Anuradha Singh
This paper aims to draw the religious life in Sarnath (and Varanasi) as accounted by the Chinese travellers-Fa-Hien and Hiuen-tsang. The accounts not only talk about the stupas, pillars, statues built by King Ashoka; vihars and monks (bhikshus) living in those vihars but also contain the first preachings of Lord Buddha, establishment of Sangha and the story of Mrigajataka that remain significant. With the increased popularity of Buddha dharma in China, the Chinese were attracted towards travelling to India. They came to India mainly with the intentions to visit the places related to the fond memories of Lord Buddha, to study the Buddha religion and philosophy and carry the copies of the Buddhist compositions. Fa-Hien and Hiuen-tsang occupy significant places among these Chinese travellers. These accounts can be associated with ancient history as well as with historical geography, religion and philosophy. While Fa-hien in his journey details had described about the Buddha Empire, Hiuen-tsang highlighted the civilisation of India and its cultural landscape, albeit it has been often accepted by the historians that these accounts of their journeys should be considered as significant only when they are backed by historical evidences. They opine that these travellers were mainly influenced by the Buddha dharma and therefore, their accounts are liable to containing exaggerated journey details. It is true that the journey details contain few imaginary instances; nevertheless, these accounts have been validated by the remnants, stupas and vihars at the sites.
In silico and network pharmacology analysis of fucosterol: a potent anticancer bioactive compound against HCC
(Springer, 2024) Kajal Singh; Pradeep Kumar; Amit Kumar Singh; Nancy Singh; Sakshi Singh; Kavindra Nath Tiwari; Shreni Agrawal; Richa Das; Anuradha Singh; Bhuwal Ram; Amit Kumar Tripathi; Sunil Kumar Mishra
The Fucaceae family of marine brown algae includes Ascophyllum nodosum. Fucosterol (FSL) is a unique bioactive component that was identified through GC-MS analysis of the hydroalcoholic extract of A. nodosum. Fucosterol's mechanism of action towards hepatocellular cancer was clarified using network pharmacology and docking study techniques. The probable target gene of FSL has been predicted using the TargetNet and SwissTargetPred databases. GeneCards and the DisGNet database were used to check the targeted genes of FSL. By using the web programme Venny 2.1, the overlaps of FSL and HCC disease demonstrated that 18 genes (1.3%) were obtained as targeted genes Via the STRING database, a protein–protein interaction (PPI) network with 18 common target genes was constructed. With the aid of CytoNCA, hub genes were screened using the Cytoscape software, and the targets' hub genes were exported into the ShinyGo online tool for study of KEGG and gene ontology enrichment. Using the software AutoDock, a hub gene molecular docking study was performed. Ten genes, including AR, CYP19A1, ESR1, ESR2, TNF, PPARA, PPARG, HMGCR, SRC, and IGF1R, were obtained. The 10 targeted hubs docked with FSL successfully. The active components FSL of ASD, the FSL, are engaged in fatty liver disease, cancer pathways, and other signalling pathways, which could prove beneficial for the management of HCC. © The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2024.
Intersecting Oppressions: The Lived Realities of Dom Women in Banaras, India
(ACCB Publishing, 2025) Anuradha Singh; Rana Abhyendra Singh; Prateek Gupta; Vishal Singh Bhadauriya
This research presents a theory-driven, qualitative account of how caste and gender work together to shape structural oppression in the lives of Dom women in Banaras (Varanasi), India. Based on fifteen in-depth interviews and approximately eighty hours of participant observation in the cremation settlements adjacent to the Manikarnika and Harishchandra Ghats, the research adopts an intersectional lens rooted in feminist standpoint epistemology. Inductive coding of verbatim transcripts identifies three mutually reinforcing mechanisms—affective discipline (gendered coercion through gratitude and fear), spatial stigma (postal-code proxies for ritual pollution), and metabolic inequality (unequal exposure to toxic ash, bleach, and smoke) — that lock Dom women into hazardous, low-wage niches of the informal economy. Compared with both upper-caste women and Dom men, participants experience significantly higher rates of occupational injury, wage theft and everyday humiliation, confirming that caste-gender oppression is not additive but synergistic. The findings refine intersectionality theory by specifying how “purity” logics operate as a caste-gender gearbox in a South-Asian urban context. By foregrounding the voices of one of India’s most marginalised constituencies, the study demonstrates why adequate social protection must address caste and gender simultaneously, rather than in parallel. © 2025 Singh et al. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Isolation, Characterization and In vivo Evaluation of Therapeutic Potential of Bacteriophage Sal11TP against Salmonella enterica Serovar Paratyphi A
(Journal of Pure and Applied Microbiology, 2025) Pratibha Goyal; Nupur Mathur; Anuradha Singh; Kushagri A. Singh; Irshad Mohammad
Salmonella Paratyphi A, also known as typhoidal Salmonella, is the causative agent of typhoid fever or paratyphoid fever, a threatening, invasive (bacteraemia), and occasionally fatal human disease (also called enteric fever). Even though Salmonella infections can be treated with different antibiotics, developing resistance to many broad-range antibiotics like chloramphenicol, nalidixic acid, ampicillin, and sulfamethoxazole is still a big problem. Therefore, an alternative strategy is needed urgently, which is more effective with minimal systemic side effects for treating diseases caused by Salmonella paratyphi A. In this current study, we describe the isolation, characterization, and in vivo evaluation of Sal11TP, a host-specific bacteriophage with lytic activity against multidrug-resistant Salmonella paratyphi A. Morphological examinations revealed that phage Sal11TP belonged to the order caudovirales of the Siphoviridae family, with an icosahedral head (62.8 nm) and a long tail (104.5 nm in length). Based on the one-step growth curve, Sal11TP has a short latent period (20 min) and burst size (29 PFU/ cell). The in vitro stability test showed that it remained stable below 40 °C and pH 6-7 after treatment for 1 h. The ideal multiplicity of infection for phage Sal11TP was 0.001. The therapeutic potential of phage Sal11TP was evaluated using a mouse model. Findings of the study demonstrated a reduction in bacterial loads in the heart and kidney tissues of intraperitoneally infected mice during prophylaxis, as well as a dose-dependent and antibiotic versus phage treatment. The explanation of the characteristics and in vivo research results of phage Sal11TP show that it could be used as an effective bio-control agent to prevent Salmonella paratyphi A infections. Phage Sal11TP’s characteristics and in vivo results suggest it could serve as an effective bio-control agent against Salmonella paratyphi A infections © The Author(s) 2025.
Resisting colonial and feudal oppression: the Bhil revolts in British Raj Rajasthan and their impact on India's freedom movement
(Discover, 2025) Vishal Singh Bhadauriya; Anuradha Singh; Rituparna Bhattacharyya
This research paper examines the significant yet often overlooked revolts of the Bhil tribe in Rajasthan during the British Raj, focusing on their resistance against colonial and feudal oppression from the early 19th to the early twentieth century. The Bhils, an indigenous tribal community, faced severe encroachments on their traditional rights, autonomy, and livelihoods due to British colonial policies and exploitative practices by Rajput feudal lords. Through an in-depth analysis of key events such as the Bhil revolts in Udaipur State (1818, 1881–1882) and insurrections in Banswara, the study highlights these uprisings as powerful manifestations of resistance by oppressed groups against systemic marginalization. The paper also explores how these tribal revolts influenced and inspired broader movements within India's struggle for independence. © The Author(s) 2025.
The Patterns of Bone Technology in Ancient Kashi (1300 bc to 300 ad)
(SAGE Publications Inc., 2021) Anuradha Singh
The political, socio-economic and cultural development of Kashi was never blocked. The history of technological development in Kashi state has been very flourished. The present study is an attempt to present historical and analytical studies regarding bone technology and its characteristics used in the region of ancient Kashi. The contribution of bone technology in the wisdom of Kashi and the development of a socio-economic perspective has also been discussed. Various bone tools obtained from Kashi’s archaeological sites and excavations reports have been studied. Archaeological and literary sources revealed that ancient Kashi was very developed in technology. The sources candidly depicted the prosperous societal life of its inhabitants in the backdrop of rich culture. Bone objects remains constitute an essential theme to study the integrated ecological aspect of human life. © 2021 Indian Council of Historical Research.