Browsing by Author "Anurag Jyoti"

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Cypermethrin alters the expression profile of mrnas in the adult rat striatum: A putative mechanism of postnatal pre-exposure followed by adulthood re-exposure-enhanced neurodegeneration
(2012) Manindra Nath Tiwari; Anand Kumar Singh; Sonal Agrawal; Satya Prakash Gupta; Anurag Jyoti; Rishi Shanker; Om Prakash; Mahendra Pratap Singh
Abstract This study was undertaken to investigate the effect of cypermethrin on the expression patterns of mRNAs in the striatum of adulthood alone and postnatal pre-exposed followed by adulthood re-exposed rats using discover chips rat microarrays. The expression patterns of V-akt murine thymoma viral oncogene homolog 1, B-cell lymphoma 2 (BCL-2), BCL-2-associated X protein, caspase 1, caspase 9, death-associated protein 3 and interleukin-1β were validated by the qRT-PCR. The expressions of inducible nitric oxide synthase (iNOS) and major histocompatibility complex (MHC) II were assessed immunohistochemically; however, tumour protein p53 and cytochrome c (mitochondrial and cytosolic) expressions were checked at protein level by western blotting. Cypermethrin differentially regulated 65 transcripts at one or the other stage of exposure and 21 transcripts exhibited more pronounced alterations in the postnatal pre-exposed and adulthood re-challenged rats. The results of qRT-PCR were in accordance with the microarray observations and the expressions of iNOS, p53 and cytosolic cytochrome c and MHC II positivity were increased while the level of mitochondrial cytochrome c was reduced in adulthood treated animals. The effects were more pronounced in the postnatal pre-exposed followed by adulthood re-exposed rats. The results obtained thus suggest that multiple pathways are involved in the neurodegeneration as well as in enhancing the vulnerability of neurons in cypermethrin pre-exposed postnatal animals upon re-exposure during adulthood. ©Springer Science+Business Media, LLC 2012.
Involvement of multiple molecular events in pyrogallol-induced hepatotoxicity and silymarin-mediated protection: Evidence from gene expression profiles
(2010) Ghanshyam Upadhyay; Manindra Nath Tiwari; Om Prakash; Anurag Jyoti; Rishi Shanker; Mahendra Pratap Singh
In this study, the involvement of various molecular events in pyrogallol-mediated hepatotoxicity was deciphered by differential mRNA transcription profiles of control and pyrogallol treated mice liver. The modulatory effects of silymarin on pyrogallol-induced differentially expressed transcripts were also looked into. Swiss albino mice were treated with or without pyrogallol. In some set of experiments, mice were also treated with silymarin 2. h prior to pyrogallol. Total RNA was isolated from liver and polyadenylated RNA was reverse-transcribed into Cye 3 or Cye 5 labelled cDNA. Equal amounts of labelled cDNA from two different groups were mixed and hybridized with mouse 15. k array. The hybridized arrays were scanned, analyzed and the expression level of each transcript was calculated. The differential expression was validated by quantitative real time polymerase chain reaction. Comparative transcription pattern showed an alteration in the expression of 183 transcripts (150 up-regulated and 33 down-regulated) associated with oxidative stress, cell cycle, cytoskeletal network, cell-cell adhesion, extra-cellular matrix, inflammation, apoptosis, cell-signaling and intermediary metabolism in pyrogallol-exposed liver and silymarin pre-treatment modulated the expression of many of these transcripts. Results obtained thus suggest that pyrogallol induces multiple molecular events leading to hepatotoxicity and silymarin effectively counteracts pyrogallol-mediated alterations. © 2010 Elsevier Ltd.
Rapid detection of enterotoxigenic Escherichia coli gene using bio-conjugated gold nano-particles
(2011) Anurag Jyoti; Surinder P. Singh; Madhu Yashpal; Premendra D. Dwivedi; Rishi Shanker
In this study, a simple gold nanoparticle based colorimetric detection system for on site diagnostics of Enterotoxigenic Escherichia coli (ETEC) has been developed. The developed assay could detect LT1 gene as evident from UV-visible spectra, the broadening of peak on hybridization of GNP probes with different copy numbers of the target gene.The present study suggests that hybridization induced changes in optical properties of GNPs can be translated into a rapid and simple colorometric" spot and read test detection method for ETEC. Copyright © 2011 American Scientific Publishers All rights reserved.