Browsing by Author "Anushka Yadav"

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A contemporary overview on quantum dots-based fluorescent biosensors: Exploring synthesis techniques, sensing mechanism and applications
(Elsevier B.V., 2025) Anushka Yadav; Priyanka Dogra; Pinky Sagar; Monika Srivastava; Amit Kumar Srivastava; Rajneesh Kumar; Sanjay Kumar Srivastava
In the epoch of bioinformatics, pivotal biomedical scrutiny and clinical diagnosis hinge upon the unfolding of highly efficacious biosensors for intricate and targeted identification of specific biomolecules. In pursuit of developing robust biosensors endowed with superior sensitivity, precise selectivity, rapid performance, and operational simplicity, semiconductor QDs have been acknowledged as pivotal and advantageous entities. In this review, we present a comprehensive analysis of the latest unfolding within the domain of QDs used in fluorescent biosensors for the detection of diverse biomolecular entities, encompassing proteins, nucleic acids, and a range of small molecules, with an emphasis on the synthesis methodologies of QDs employed and mechanism behind sensing. Additionally, this review delves into several pivotal facets of QD-based fluorescent biosensors in detail, such as surface functionalization methodologies aimed at enhancing biocompatibility and improving target specificity. The challenges and future perspectives of QD-based fluorescent biosensors are also considered, emphasizing the necessity of ongoing multidisciplinary research to realize their full potential in enhancing personalized medicine and biomedical diagnostics. © 2025 Elsevier B.V.
A smartphone-enabled colorimetric sensor based on VS2 quantum dots for Rapid and on-site detection of ferric ions
(Elsevier B.V., 2025) Anushka Yadav; Pinky Sagar; Monika Srivastava; Amit Kumar Srivastava; Rajneesh Kumar; Sanjay Kumar Srivastava
This research delves into the holistic hydrothermal synthesis of VS2 QDs and their subsequent utilization as a fluorescent probe for the subtle detection of ferric ions (Fe3+) in practical sample matrices. The detection paradigms harness a colorimetric sensing mechanism, amplified by smartphone-enabled analytical integration for improved precision and real-time monitoring. A comprehensive suite of analytical characterization techniques has been employed, revealing that the as-synthesized VS2 QDs feature a surface densely populated with functional groups. While the VS2 QDs showcase interactions with multifarious metal ions in aqueous media, they set forth a pronounced and selective fluorescent quenching response toward Fe3+ ions, markedly surpassing their interactions with other metal ions. The developed sensing probe exhibits a linear detection range spanning from 0 – 90 μM, with a LOD as low as 2.25 μM, also exhibits exceptional sensitivity (KD ∼ 0.8 × 104 M−1) and remarkable selectivity for Fe3+ ions, harnessing the intrinsic photoluminescent characteristics of VS2 QDs. In addition, a sophisticated portable smartphone platform, integrated with a radiometric fluorescence probe specifically tailored for in-situ detection of Fe3+ at the point of care, exhibits a LOD of approximately 5.05 μM, a value that resides below the prescribed safety threshold. Thus, the proposed probe stands to function as an exceptionally potent sensing apparatus for the precise quantification of Fe3+ in complex real-world samples. © 2024 Elsevier B.V.