Browsing by Author "Arpita Ghosh"

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Comparative whole genome analysis of Listeria monocytogenes 4b strains reveals least genome diversification irrespective of their niche specificity
(Elsevier Inc, 2017) Dharmendra K. Soni; Arpita Ghosh; Surendra K. Chikara; Krishna M. Singh; Chaitanya G. Joshi; Suresh K. Dubey
Listeria monocytogenes has emerged as the deadly pathogen inflicting high mortality in humans and animals. To investigate the strain-specific characteristics of L. monocytogenes, and their role(s) in virulence and ecological sustenance, we sequenced genome of three L. monocytogenes strains BHU1, 2 and 3 isolated from the Ganges river, agricultural soil and human placenta bit, respectively, and compared it with L. monocytogenes EGD-e serovar 1/2a and L. monocytogenes F2365 serovar 4b strain. The contigs of all the three strains had a similarity (> 90%) in regions that aligned with EGD-e and F2365. A total of 2872 core genes on the set of strains were identified in BHU1, 2 and 3 strains. In the mice virulence assay, BHU2 and 3 strains showed pathogenicity while BHU1 was non-pathogenic. These strains were also characterized with unique genes (8 genes in BHU1, 12 in BHU 2, and 17 in BHU 3 strain). Phylogenetic analysis based on multilocus sequence typing revealed BHU1, 2 and 3 strains to be more closely related to lineage I, serotype 4b, clonal complex 1, and sequence type 328. BHU1 strain seemed to harbor nucleotide mutation from A to G in the major virulence genes i.e. hemolysin D and listeriolysin O. Strain-specific mutations 8, 5 and 2 were identified in BHU1, 2 and 3, respectively, compared to F2365. Though all the three strains were genetically very close, the observed differences may play the crucial role(s) in their virulence attributes, and also, in the prevalence of L. monocytogenes. © 2017 Elsevier Inc.
Enabling Environment for Climate-Smart Agriculture: A Critical Review of Climate Smart Practices from South Asia and Sub-Saharan Africa
(Technoscience Publications, 2025) Arpita Ghosh; Puneet Sharma; Arnab Mondal; Surajit Mondal
In South Asian and Sub-Saharan African nations, climate change offers numerous hurdles to growth and development. These regions are susceptible to climate change due to their vast population reliance on agriculture, high demand for natural resources, and comparatively limited strategies for coping. Reduced food grain yields, crop losses, feed scarcity, lack of potable water for livestock during the summer, forceful animal migrations, and severe losses in the poultry and fishery industries have all been documented, posing a threat to the lives of the rural poor. As global food security and agricultural productivity become increasingly vulnerable, the focus has shifted towards adopting climate-smart agricultural practices and techniques. The present study discussed the need to identify and prioritize regionally evolving climate-smart farming practices and the enabling environment required for CSA uptake. The popular CSA practices in South Asia and Sub-Saharan Africa are crop rotation, cultivation of drought/flood-tolerant crops, legume intercropping, changing planting dates, rainwater harvesting, agroforestry, micro-irrigation technologies, minimum tillage, and integrated crop-livestock farming. A solid institutional structure, policy environment, infrastructure, agricultural insurance, climate information services, and gender and social inclusion provide the required enabling environment to alleviate farmer issues, lower CSA adoption obstacles, and improve operational sustainability. Highlights of the study are: This study examines how climate-smart farming practices are evolving in South Asia and Sub-Saharan Africa. We used a systematic approach to categorize and characterize agricultural adaptation alternatives to climate change. Our specific goals are to gain knowledge of the CSA adoption-enabling environments and the climate-smart agriculture practices employed in South Asia and Sub-Saharan Africa. © 2025 by the authors.
Nonmedical factors and health-related quality of life in CKD in India
(American Society of Nephrology, 2020) Gopesh K. Modi; Ashok K. Yadav; Arpita Ghosh; Kajal Kamboj; Prabhjot Kaur; Vivek Kumar; Shobhit Bhansali; Narayan Prasad; Manisha Sahay; Sreejith Parameswaran; Santosh Varughese; Sishir Gang; Shivendra Singh; Dipankar Sircar; Natarajan Gopalakrishnan; Ajay Jaryal; Sanjay Vikrant; Seema Baid Agarwal; Vivekanand Jha
Background and objectives Patient-reported outcomes have gained prominence in the management of chronic noncommunicable diseases. Measurement of health-related quality of life is being increasingly incorporated into medical decision making and health care delivery processes. Design, setting, participants, & measurements The Indian Chronic Kidney Disease Study is a prospective cohort of participants with mild to moderate CKD. Baseline health-related quality of life scores, determined by the standardized Kidney Disease Quality of Life 36 item instrument, are presented for the inception cohort (n52919). Scores are presented on five subscales: mental component summary, physical component summary, burden, effect of kidney disease, and symptom and problems; each is scored 0–100. The associations of socioeconomic and clinical parameters with the five subscale scores and lower quality of life (defined as subscale score <1 SD of the sample mean) were examined. The main socioeconomic factors studied were sex, education, occupation, and income. The key medical factors studied were age, eGFR, diabetes, hypertension, and albuminuria. Results The mean (SD) subscale scores were physical component summary score, 4369; mental component summary score, 48610; burden, 61633; effects, 87613; and symptoms, 90620. Among the socioeconomic variables, women, lower education, and lower income were negatively associated with reduced scores across all subscales. For instance, the respective b-coefficients (SD) for association with the physical component summary subscale were 22.6 (23.4 to 21.8), 21.5 (22.2 to 20.7), and 21.6 (22.7 to 20.5). Medical factors had inconsistent or no association with subscale scores. The quality of life scores also displayed regional variations. Conclusions In this first of its kind analysis from India, predominantly socioeconomic factors were associated with quality of life scores in patients with CKD. © 2020 by the American Society of Nephrology.
Prescription Practices in Patients With Mild to Moderate CKD in India
(Elsevier Inc., 2021) Narayan Prasad; Ashok Kumar Yadav; Monica Kundu; Jasmin Sethi; Ajay Jaryal; Dipankar Sircar; Gopesh K. Modi; Kajal Kamboj; Manisha Sahay; Natarajan Gopalakrishnan; Prabhjot Kaur; Sanjay Vikrant; Santosh Varughese; Seema Baid-Agrawal; Shivendra Singh; Sishir Gang; Sreejith Parameswaran; Vivek Kumar; Arpita Ghosh; Vivekanand Jha
Introduction: Patients with chronic kidney disease (CKD) require multiple medications. There is no information on prescription patterns or the use of evidence-based therapies for management of CKD from low-middle-income countries. Using baseline data from the Indian CKD (ICKD) cohort, we describe the drug prescription practices in patients with mild to moderate CKD. Methods: The ICKD study is a prospective, observational cohort study of mild to moderate kidney disease across 11 centers in India. We analyzed all the prescriptions captured at enrollment in the ICKD study. Drugs were categorized into 11 different groups. We provide descriptive data on prescription details and evaluate the appropriateness of medication use. Results: Complete prescription data were available in 3966 out of 4056 (97.8%) subjects enrolled in the ICKD database. Most patients had stage 3 CKD, 24.9% had diabetic kidney disease, 87% had hypertension, and 25.5% had moderate to severe proteinuria. Renin-angiotensin-aldosterone system blockers were prescribed in less than half (47.9%) and in 58.8% of patients with proteinuric CKD. Metformin was prescribed in 25.7% of diabetic subjects with CKD. Only 40.4% of patients were taking statins; 31.1% and 2.8% subjects with anemia were receiving iron and erythropoiesis-stimulating agents, respectively. Conclusion: This study highlights the missed opportunities for improving outcomes through appropriate prescriptions of drugs in patients with CKD. There is need for dissemination of evidence-based guidelines and institution of sustainable implementation practices for improving the overall health of patients with CKD. © 2021 International Society of Nephrology
Renin-angiotensin blocker use is associated with improved cardiovascular mortality in Indian patients with mild-moderate chronic kidney disease—findings from the ICKD study
(Frontiers Media S.A., 2022) Narayan Prasad; Ashok Kumar Yadav; Monica Kundu; Ajay Jaryal; Dipankar Sircar; Gopesh Modi; Manisha Sahay; Natarajan Gopalakrishnan; Sanjay Vikrant; Santosh Varughese; Seema Baid-Agrawal; Shivendra Singh; Sishir Gang; Sreejith Parameswaran; Arpita Ghosh; Vivek Kumar; Vivekanand Jha
Introduction: Angiotensin-converting enzyme inhibitors (ACEI) and angiotensin receptor blockers (ARB) are the antihypertensive drug class of choice in patients with chronic kidney disease (CKD). Head-to-head comparisons of the renal or non-renal outcomes between ACEI/ARB users and nonusers have not been conducted in all population groups. We examined the renal and cardiovascular outcomes in users and nonusers enrolled in the Indian Chronic Kidney Disease (ICKD) Study. Methods: A total of 4,056 patients with mild-moderate CKD were studied. Patients were categorized as ACEI/ARB users or nonusers. Major adverse kidney events [ESKD (end stage kidney disease), ≥50% decline in eGFR and kidney death], all-cause mortality, and cardiovascular mortality were analyzed over a median follow-up period of 2.64 (1.40, 3.89) years between the two groups. Results: Out of a total of 4,056 patients, 3,487 (87%) were hypertensive. The adjusted sub-hazard ratio (SHR) and 95 % CI for ACEI /ARB users was 0.85 (0.71, 1.02) for MAKE, 0.80 (0.64, 0.99) for a 50% decline in eGFR, and 0.72 (0.58, 0.90) for ESKD. For cardiovascular mortality, ACEI/ARB users were at lower risk (SHR = 0.55, 95% CI: 0.34, 0.88). Diuretic users were at increased risk of all-cause mortality (HR = 1.95, 95% CI: 1.50, 2.53) and cardiovascular mortality (adjusted SHR = 1.73, 95% CI: 1.09, 2.73). There was non-significant association between the use of other antihypertensives and any of the end points. Discussion: ACEI/ARB use is associated with slower rate of decline in eGFR in those with CKD stage 1-3. ACEI/ARB users had a significantly lower risk of renal outcomes, and cardiovascular mortality. Copyright © 2022 Prasad, Yadav, Kundu, Jaryal, Sircar, Modi, Sahay, Gopalakrishnan, Vikrant, Varughese, Baid-Agrawal, Singh, Gang, Parameswaran, Ghosh, Kumar and Jha.
Socio-economic-environmental challenges at himachal villages: findings from five unnat bharat abhiyan adopted villages
(Springer Science and Business Media Deutschland GmbH, 2024) Arpita Ghosh; Puneet Sharma; Devika Vashisht; Parul Malik; Arnab Mondal; Surajit Mondal
This research pinpoints the primary socio-economic issues and inherent circumstances of rural development in Himachal Pradesh, India. It provides a critical analysis of 289 households based on the surveys conducted in 5 villages of Sirmaur district, adopted under the UBA (‘Unnat Bharat Abhiyan’, transl. ‘Developed India initiative’). This study highlights and evaluates the significant socio-economic concerns and rural development challenges based on key factors such as population, education, employment, rural development schemes, livestock assets, property distribution, essential services and transport connectivity. The findings from this study are crucial for identifying priority areas for community capacity building and sustainable rural development including literacy rates, healthcare, and agricultural products. The study emphasizes the prudent use of existing resources and the maximisation of benefits from centrally and state-sponsored schemes for the comprehensive development of the adopted villages under UBA. Although the current initiative is based on the UBA-adopted villages, it may be applied to other rural villages of Himalayan states. This article highlights inter-variations in socio-economic issues such as healthcare, sanitation, waste management, unsustainable agriculture methods, drug misuse, unemployment, and sanitation in the UBA adopted villages in the Sirmaur District, Himachal Pradesh, India. © 2024, The Author(s), under exclusive licence to Springer Nature B.V.
The Indian Chronic Kidney Disease (ICKD) study: baseline characteristics
(Oxford University Press, 2022) Vivek Kumar; Ashok Kumar Yadav; Jasmine Sethi; Arpita Ghosh; Manisha Sahay; Narayan Prasad; Santosh Varughese; Sreejith Parameswaran; Natarajan Gopalakrishnan; Prabhjot Kaur; Gopesh K. Modi; Kajal Kamboj; Monica Kundu; Vivek Sood; Neeraj Inamdar; Ajay Jaryal; Sanjay Vikrant; Saurabh Nayak; Shivendra Singh; Sishir Gang; Seema Baid-Agrawal; Vivekanand Jha
Background: Chronic kidney disease (CKD) is an important cause of morbidity and mortality worldwide. There is a lack of information on epidemiology and progression of CKD in low-middle income countries. The Indian Chronic Kidney Disease (ICKD) study aims to identify factors that associate with CKD progression, and development of kidney failure and cardiovascular disease (CVD) in Indian patients with CKD. Methods: ICKD study is prospective, multicentric cohort study enrolling patients with estimated glomerular filtration rate (eGFR) 15-60 mL/min/1.73 m2, or >60 mL/min/1.73 m2 with proteinuria. Clinical details and biological samples are collected at annual visits. We analysed the baseline characteristics including socio-demographic details, risk factors, disease characteristics and laboratory measurements. In addition, we compared characteristics between urban and rural participants. Results: A total of 4056 patients have been enrolled up to 31 March 2020. The mean ± SD age was 50.3 ± 11.8 years, 67.2% were males, two-thirds of patients lived in rural areas and the median eGFR was 40 mL/min/1.73 m2. About 87% were hypertensive, 37% had diabetes, 22% had CVD, 6.7% had past history of acute kidney injury and 23% reported prior use of alternative drugs. Diabetic kidney disease, chronic interstitial nephritis (CIN) and CKD-cause unknown (CKDu) were the leading causes. Rural participants had more occupational exposure and tobacco use but lower educational status and income. CIN and unknown categories were leading causes in rural participants. Conclusions: The ICKD study is the only large cohort study of patients with mild-to-moderate CKD in a lower middle income country. Baseline characteristics of study population reveal differences as compared with other cohorts from high-income countries. © 2021 The Author(s) 2021.
Whole-genome sequence of Listeria monocytogenes strains from clinical and environmental samples from Varanasi, India
(American Society for Microbiology, 2015) Dharmendra K. Soni; Krishna M. Singh; Arpita Ghosh; Surendra K. Chikara; Chaitanya G. Joshi; Suresh K. Dubey
We present here the whole-genome sequences of Listeria monocytogenes from Ganges River water, agricultural soil, and human clinical samples from Varanasi, India, which will be used for a comparative analysis. © 2015 Soni et al.