Browsing by Author "Arti Singh"

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Advances in extraction technologies: Isolation and purification of bioactive compounds from biological materials
(Elsevier, 2020) Nishi Srivastava; Arti Singh; Puja Kumari; Jay Hind Nishad; Veer Singh Gautam; Monika Yadav; Rajnish Bharti; Dharmendra Kumar; Ravindra N. Kharwar
Natural bioactive compounds (NBCs) are receiving more popularity because of their various medicinal properties and huge exploitation in various commercial sectors such as food, chemical, and pharmaceutical industries. The extraction of NBCs begins with the selection of a suitable protocol, sample preparation, and extensive literature survey. During the extraction of NBCs from biological materials, the major concern of researchers is on minimizing the interference of unwanted compounds that may coextract with the focused compounds. Numerous extraction procedures have been introduced along with existing classical extraction methodology (conventional extraction methodology), but still, scientists are in search of the development of a standard single method for extracting NBCs from biological materials. There are several critical parameters such as understanding the nature of the plant matrix and chemistry of bioactive compounds and scientific expertise that impact efficiencies of conventional and nonconventional extraction. This chapter focuses on the mechanism of different extraction techniques, extraction instrumentation, and common problems encountered and their elimination. For efficient extraction of NBCs from biological materials, there is an urgent need for advancement in selective extraction procedures, from extraction to fractionation and subsequent purification that will speed up and scale up the isolation of purified compounds from biological materials. © 2021 Elsevier Inc.
Antifungal, Antiaflatoxin and Antioxidant Activity of Plant Essential Oils and Their In Vivo Efficacy in Protection of Chickpea Seeds
(Blackwell Publishing Ltd, 2016) Bhanu Prakash; Akash Kedia; Aakanksha Singh; Shashi Yadav; Arti Singh; Amrita Yadav; Deepika; Nawal Kishore Dubey
This study presents the efficacy of six essential oils (EOs), viz. Carum carvi, Myristica fragrans, Melaleuca leucadendra, Cinnamomum camphora, Pelargonium odoratissimum and Cymbopogon citratus, as food preservatives based on antifungal, antiaflatoxin and antioxidant activity. The minimum inhibitory concentration of EOs against aflatoxigenic strain of Aspergillus flavus (LHP-10) and 13 other storage molds ranged between 1.25 and 6.0μL/mL, while aflatoxin inhibition was observed at 1.0-5.0μL/mL. EOs also exhibited free-radical scavenging activity through DPPH (2,2-diphenyl-1-picrylhydrazil) assay as IC50 value ranged between 3.96 and 96.63μL/mL. The antifungal action of EOs was observed in terms of reduction in ergosterol content of the plasma membrane of A.flavus. The EOs provided >50% protection of chickpea samples against fungal association without affecting their germination during in vivo testing in storage containers. Based on antifungal, antiaflatoxigenic, antioxidant potential and in vivo efficacy, the tested EOs may be recommended as plant-based food preservatives. © 2016 Wiley Periodicals, Inc.
Bioactive potential evaluation and purification of compounds from an endophytic fungus Diaporthe longicolla, a resident of Saraca asoca (Roxb.) Willd.
(Springer Science and Business Media Deutschland GmbH, 2021) Jay Hind Nishad; Arti Singh; Veer Singh Gautam; Puja Kumari; Jitendra Kumar; Monika Yadav; Ravindra Nath Kharwar
An endophytic fungus (L3), isolated from the leaf tissues of Saraca asoca was identified as D. longicolla by microscopic and molecular methods. The crude extracts of D. longicolla revealed to harbor seven compounds in GC–MS analysis which was subjected to a thin layer chromatography (TLC) for purification and separation of bioactive ingredients. The partially purified fraction from TLC displayed the presence of 2-tridecene (Z) (RT-14.50), 5-tridecene (E) (RT-16.65) and 2,4-di-tert-butylphenol (RT-13.92) in GC–MS. High-performance liquid chromatography (HPLC) was performed to further purify the constituents which led to the collection of 2,4-di-tert-butyl phenol (RT-2.34) with excellent antioxidant activity and antibacterial activity against methicillin resistance Staphylococcus aureus (MRSA). © 2021, The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.
Effect of the Histone Methyltransferase Specific Probe BRD4770 on Metabolic Profiling of the Endophytic Fungus Diaporthe longicolla
(Frontiers Media S.A., 2021) Jay Hind Nishad; Arti Singh; Rajnish Bharti; Priyanka Prajapati; Vijay Kumar Sharma; Vijai Kumar Gupta; Ravindra Nath Kharwar
The endophytic fungus Diaporthe longicolla was isolated from the stem of Saraca asoca (Roxb.) Willd., commonly known as Ashok plant in India and Sri Lanka. Since no reports are available regarding epigenetic modulations by BRD4770 in microbial entities, D. longicolla was treated with different concentrations of BRD4770 for this purpose and evaluated for its antioxidant and antibacterial potential against five human pathogenic bacteria, Staphylococcus aureus, methicillin-resistant Staphylococcus aureus (MRSA), Shigella boydii, Klebsiella pneumoniae, and Escherichia coli. The crude extract obtained from cultures treated with 100 nM concentration of BRD4770 showed increased antioxidant activity and inhibition zone against S. aureus and MRSA, compared to the non-treated control. The composition of the non-treated and treated crude extract was analyzed, and induced compounds were identified with the help of Gas chromatography–mass spectrometry (GC-MS) and LC-ESI-MS/MS. LC-ESI-MS/MS analysis showed that berberine (antibacterial)-, caffeine-, and theobromine (antioxidant)-like compounds were induced in the BRD4770-treated crude extract. The presence of particular absorbance at a wavelength of 346.5 nm for berberine, 259.4 nm for caffeine, and 278.4 nm for theobromine in the reverse-phase high-performance liquid chromatography (HPLC) analysis of both BRD4770-treated crude metabolites and standard solution of the above compounds strongly supported the increased antibacterial and antioxidant activities that may be due to inducing the alterations in bioactivities of the BRD4770-treated culture. © Copyright © 2021 Nishad, Singh, Bharti, Prajapati, Sharma, Gupta and Kharwar.
Endophytic Fungi: A Cryptic Fountainhead for Biodiversity, Functional Metabolites, Host Stress Tolerance, and Myco-mediated Nanoparticles (Nps) Synthesis
(Springer Science and Business Media B.V., 2019) Jay Hind Nishad; Arti Singh; Veer Singh Gautam; Dharmendra Kumar; Jitendra Kumar; R.N. Kharwar
The view on association of higher plants and fungi from past few decades confirms the belief that all plants foster their own endophytic fungal diversity as a host, and all plant species studied till date are found to harbor one or more endophytes. The diversity of endophytic fungi can have deep impressions on plant communities through adding fitness to their concerned host conferring tolerance against abiotic and biotic stresses. While endophytes have been outlined to biosynthesize a wide array of molecules, genome sequencing of such organisms has revealed that these have the potential to provide many more secondary metabolites than usual. Recently, various methods have been advanced to aid in the activation of cryptic biosynthetic pathways. Since the most important medicinal compound taxol (paclitaxel) has been isolated from the endophytic fungus therefore, more plant mimetic compounds may be expected from this hidden microbial source. Various enzymes (amylase, lipase, cellulase, protease, lactase, pectinases, peroxidase, catalase, and penicillinase) and toxins (aflatoxin, zearalenone, ochratoxin, citrinin, T-2 toxin, and fumonisins) may be isolated from this repertoire. Cell-free extract of many endophytic fungal isolates may also be utilized to synthesize the nanoparticles like copper (Cu), silver (Ag), platinum (Pt), and gold (Au) from respective metal salt solutions. This chapter also discusses different approaches such as co-culture of microbes, altering growth media and culture conditions, genetic as well as epigenetic strategies for obtaining the biochemical treasure hidden within these unique microbes. © 2019, Springer Nature Switzerland AG.
Evaluation of Congo red dye decolorization and degradation potential of an endophyte Colletotrichum gloeosporioides isolated from Thevetia peruviana (Pers.) K. Schum.
(Springer Science and Business Media B.V., 2023) Rajnish Bharti; Monika Yadav; Arti Singh; Puja Kumari; Jay Hind Nishad; Veer Singh Gautam; Ravindra Nath Kharwar
Decolorization and degradation of textile dye by endophytic fungi stand to be a profitable and viable alternative over conventional methods with respect to eco-friendliness, cost-effectiveness, and non-hazardous nature. One of the active fungal endophytes Colletotrichum gloeosporioides isolated from plant Thevetia peruviana (Pers.) K. Schum. was screened for laccase production and Congo red dye decolorization. Various physicochemical parameters like dye concentration, carbon sources, nitrogen sources, temperature, and pH were optimized, and the maximum decolorization (%) was achieved at 100 mg/L of dye concentration (82%), yeast extract (80%), 30 °C temp (80%), glucose (79%), and 7 pH (78%), respectively. SEM image and fungal biomass changes represent that fungus actively participated in the dye decolorization and had less significant effect on biomass. The regenerative ability of fungus C. gloeosporioides after dye decolorization indicated tolerance against the dye and was found to be more advantageous over previous reports of dye decolorization by other endophytic fungi. UV-Vis spectra, TLC, FTIR, and HPLC results confirmed the decolorization and degradation process due to absorption and biodegradation. Phytotoxicity assay depicted that degraded products are less toxic to Phaseolus mungo compared to Congo red. The overall findings showed that C. gloeosporioides possesses a good decolorization and degradation potential against Congo red and this endophyte can be profitably used for dye-containing wastewater treatment. © 2022, Institute of Microbiology, Academy of Sciences of the Czech Republic, v.v.i.
Interplay between food-associated oxidative stress and NDG disorders
(Elsevier, 2024) Lakshay Kapil; Vishal Kumar; Sanchit Arora; Simranjit Kaur; Sonima Prasad; Charan Singh; Arti Singh
Food-associated oxidative stress has a significant impact on the pathophysiology of many diseases and is often influenced by dietary patterns, food preferences, and nutrient intake. Unhealthy eating practices also cause the creation of free radicals, which interact with polyunsaturated fatty acids to create lipid peroxides. When these peroxides degrade, a chain of events begins that includes the production of the recognized mutagen malondialdehyde (MDA). Lipid peroxides have been demonstrated to reduce membrane permeability and flexibility, which may lead to cell damage. These changes are probably more pronounced in long-lived, primarily postmitotic cells like neurons, which may result in a variety of illnesses. In the study, several micro- and macronutrients, their origins, and associated dysfunctions are highlighted along with the mechanism underlying food-associated oxidative stress. © 2025 Elsevier Inc. All rights reserved.
Isolation and functional characterization of a fungal plant symbiont Nigrospora sphaerica, associated to Euphorbia hirta L.
(Springer, 2022) Veer Singh Gautam; Jay Hind Nishad; Puja Kumari; Arti Singh; Satish Kumar Verma; Vijay Kumar Sharma; Jitendra Kumar; Ravindra Nath Kharwar
The endophytic fungi are the endosymbiont which play important role in improving host plant fitness and source of plethora of bioactive molecules. Present study includes the assessment of antimicrobial activity, phytochemical analysis and enzymes activity of fungal endophyte EHL2, isolated from leaf tissues of an Indian medicinal plant Euphorbia hirta L. The fungus exhibited the antibacterial and antifungal activities against a broad range of bacterial and fungal pathogens. To the best of our knowledge, this is the first report about the isolation and characterization of endophytic fungus Nigrospora sphaerica (EHL2) recovered from E. hirta L. The minimum inhibitory concentration (MIC) of the crude extract against pathogenic bacteria ranged from 0.45 to 3.14 mg/ml. For antifungal ativity of fungus, the highest percentage of inhibition was observed against Colletotrichum sp. (33.78%) while, minimum activity was noticed against Alternaria solani (16.60%). Preliminary mycochemical analysis revealed the positive tests for alkaloids, phenolics, flavonoids and terpenoids. The results showed that the total phenolic content (TPC) and total flavonoid content (TFC) of crude extracts were 78.11 ± 0.04 mg GAE/g and 235.94 ± 3.06 mg RE/g, respectively. Furthermore, the fungus also produced amylase, cellulase, protease and laccase enzymes. In conclusion, these positive results of mycochemicals and enzymes activity displayed by N. sphaerica of E. hirta provide an opportunity which could be exploited for host protection against pathogens and enzyme production. © 2022, Indian Phytopathological Society.
Isolation and purification of bioactive metabolites from an endophytic fungus Penicillium citrinum of Azadirachta indica
(Elsevier B.V., 2021) Puja Kumari; Arti Singh; Dheeraj K. Singh; Vijay K. Sharma; Jitendra Kumar; Vijai Kumar Gupta; S. Bhattacharya; R.N. Kharwar
Endophytic fungi are the plant symbiont with highly diverse nature and poorly defined ecological importance in host fitness. Although there are the reports on the isolation and characterization of fungal endophytes from a variety of hosts, there is still no report of Penicillium citrinum from Azadirachta indica. In this study, an endophytic P. citrinum was isolated from A. indica. The purified fraction of secondary metabolites was characterized by combining TLC, GC-MS, 1H NMR and 13C NMR analyses. The TLC purified fraction was identified as milbemycin. The pure fraction did not show any antioxidant activity while crude extract showed strong antioxidant activity (DPPH inhibition capacity; IC50 = 52.13 μg ml−1). The secondary metabolites displayed significant antimicrobial activity against human's pathogenic bacteria and fungi. The inhibition zones between 15 and 20 mm were recorded against Gram +ve Staphylococcus aureus, Enterococcus faecalis and Gram–ve Aeromonas hydrophila, while maximum inhibition of 29 mm was observed against Trichophyton mentagrophytes. P. citrinum can be a promising fungus that has broad spectrum antimicrobial activity and may provide future insight towards the production of bioactive compounds. © 2021 SAAB
Matrix metalloproteinases (MMP-2 and MMP-9) activity in corneal ulcer and ocular surface disorders determined by gelatin zymography
(2012) Arti Singh; O.P.S. Maurya; M.V. Jagannadhan; Ashok Patel
The purpose of this paper is to determine the active form of matrix metalloproteinases (MMP-2 and MMP-9) in corneal ulcer and ocular surface disorder patients. A total of 35 patients of corneal ulcer, 20 patients of ocular surface disorders and 10 control subjects were included in this study and estimation of active form ofMMP-2 andMMP-9 was done by gelatin zymography. Tear samples were collected by capillary tube method. Both pro- and active forms of MMP-9 were detected in 24 out of 35 patients with corneal ulcer and 15 out of 20 patients with ocular surface disorders. None of the patients were showing MMP-2 activity. Neither MMP-2 nor MMP-9 was detected in the control group. Active forms of MMP-9 are present in tears of severe ulcerative and ocular surface disorder patients. Thus, proteinase inhibitors have been recommended for the treatment of corneal ulcer and ocular surface disorders to reduced the progression of stromal ulcer and to minimize corneal scarring. © Springer Science+Business Media New York 2013.
Mycosynthesis of bactericidal silver and polymorphic gold nanoparticles: Physicochemical variation effects and mechanism
(Future Medicine Ltd., 2018) Dheeraj Kumar Singh; Jitendra Kumar; Vijay Kumar Sharma; Satish Kumar Verma; Arti Singh; Puja Kumari; Ravindra Nath Kharwar
Aim: Extracellular synthesis of silver and gold nanoparticles using aqueous cell-free filtrate (CFF) of endophytic Chaetomium globosum and characterization of its bioactive proteins. Methods: Temperature and pH gradients were used to assess their effects on dimensions of NPs. NPs were tested in vivo for antibacterial activity. MALDI-TOF-MS/MS was used for characterization of CFF proteins. Results: Fungal CFF fabricated nanoparticles of various shape under varied physicochemical conditions. Silver nanoparticles showed significantly (p ≤ 0.5) enhanced antibacterial activity against Staphylococcus aureus and Klebsiella pneumoniae compared with AgNO3. Two prominent CFF proteins showed homology with benzoate 4-monooxygenase cytochrome P450 and ubiquinol-cytochrome c reductase. Conclusion: The study achieved controlled mycosynthesis of NPs and explains the hitherto poorly known mechanism of reduction, stabilization and antibacterial activity of nanoparticles. © 2018 Future Medicine Ltd.
Phenolic and flavonoid contents and antioxidant activity of an endophytic fungus Nigrospora sphaerica (EHL2), inhabiting the medicinal plant Euphorbia hirta (dudhi) L.
(Springer Science and Business Media Deutschland GmbH, 2022) Veer Singh Gautam; Arti Singh; Puja Kumari; Jay Hind Nishad; Jitendra Kumar; Monika Yadav; Rajnish Bharti; Priyanka Prajapati; Ravindra Nath Kharwar
Since endophytic fungi are pivotal sources of various bioactive natural compounds, the present study is aimed to investigate the antioxidant compounds of the endophytic fungus Nigrospora sphaerica isolated from a pantropical weed, Euphorbia hirta L. The fungus was fermented in four different media and each filtered broth was sequentially extracted in various solvents. Crude extracts collected from different solvents were subjected to phytochemical analysis and antioxidant activity. The total phenolic content (TPC) and total flavonoid content (TFC) were maximal in ethyl acetate crude extract (EtOAcE) of endophyte fermented in potato dextrose broth (PDB) medium (77.74 ± 0.046mgGAE/g and 230.59 ± 2.0 mgRE/g) with the highest 96.80% antioxidant activity. However, TPC and TFC were absent in hexane extract of Czapek Dox broth (CDB) medium exhibiting the lowest 4.63 ± 2.75% activity. The EtOAcE (PDB) showed a positive correlation between TFC and antiradical activity (R2 = 0.762; P < 0.05), whereas a high positive correlation was noticed between TPC and antioxidant activity (R2 = 0.989; P < 0.05). Furthermore, to determine the antioxidant activity, EtOAcE (PDB) was subjected to TLC bioautography-based partial purification, while GC/MS analysis of the partial purified extract was done to confirm the presence of phenolics along with antioxidant compounds that resulted in the detection of 2,4-Di-tert-butylphenol (13.83%), a phenolic compound accountable for the antioxidant potential. Conclusively, N. sphaerica is a potential candidate for natural antioxidant. Graphical abstract: [Figure not available: see fulltext.] © 2022, The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.
Phytochemical analysis and antimicrobial activity of an endophytic Fusarium proliferatum (ACQR8), isolated from a folk medicinal plant Cissus quadrangularis L.
(Elsevier B.V., 2021) Arti Singh; Jitendra Kumar; Vijay Kumar Sharma; Dheeraj Kumar Singh; Puja Kumari; Jay Hind Nishad; Veer Singh Gautam; Ravindra Nath Kharwar
The endophytic fungi are an important source of bioactive natural products. This study includes evaluation of antimicrobial activity and phytochemical analysis of endophytic fungus ACQR8, recovered from the root tissues of a folk medicinal plant Cissus quadrangularis L., which was identified as Fusarium proliferatum by microscopic and molecular methods. The fungus showed broad spectrum antibacterial and antifungal activities against variety of important plant and human pathogens. To the best of our knowledge, this is the first study to record antifungal activity of any endophytic Fusarium sp. against phytopathogens Rhizoctonia solani, Fusarium oxysporum and the dermatophytes Trichophyton mentagrophyte and Microsporum gypseum. The minimum inhibitory concentration of the crude extract ranged from 40–120 µg/ml against pathogenic bacteria and 0.2–2.5 mg/ml against fungal pathogens. Besides, this study probably is also the first to document the phytochemical constituents of an endophytic Fusarium sp. Phytochemical testing and GC-MS (gas chromatography- mass spectrometry) analysis revealed the presence of fatty acids and its derivatives, phenolics, terpenoids and unsaturated alkenes in the crude extract which scientifically validates the highly promising antibacterial and antifungal activity exhibited by endophytic isolate ACQR8. © 2021 SAAB
Phytostimulation and ISR responses of fungi
(Elsevier, 2020) Puja Kumari; Arti Singh; Ravindra Nath Kharwar
Our earth is blessed with many resources including the soil, the another world or home where various kinds of micro-organisms resides not only for their survival but also beneficiating the plants in growth development, stress tolerance capacity by the rhizofiltration of the heavy metals and other contaminants present in the soil. Soil pollution is one of the major ecological problem facing by the present modernized world as well as the plants. Fungi gives the potential to grow under stressed environment along with protection from the phytopathogens. In this chapter, we are going to discuss the different fungi responsible for the phytostimulation and Induced systemic resistance in plants. © 2021 Elsevier Inc.
Piperine loaded metal organic frameworks reverse doxorubicin induced chemobrain in adult zebrafish
(Elsevier B.V., 2023) Sanchit Arora; Vishal Kumar; Lakshay Kapil; Ashish Kumar Agrawal; Arti Singh; Charan Singh
The study's primary goal was to enhance medicinal potential of piperine (PIP)-loaded zeolitic imidazolate frameworks-8 (PIP@ZIF-8) against doxorubicin (DOX)-induced cognitive impairments in zebrafish. Herein, PIP@ZIF-8 was synthesized via easy, economical and reproducible ultrasonication method followed by spray drying technology. ZIF-8's structural integrity has been confirmed by PXRD, and even after PIP was encapsulated, the structure of ZIF-8 remained unchanged. Pure ZIF-8 and PIP@ZIF-8 were subjected to TEM analysis, which revealed hexagonal morphology with a nanosize range. FTIR and UV–Visible spectroscopy studies confirmed the drug loading of ZIF-8. Studies on in vitro release revealed 71.48 ± 7.21% and 34.56 ± 5.35% PIP release from PIP@ZIF-8 and unformulated PIP, respectively in pH 7.4. The highest antioxidant scavenging results were obtained with vitamin C (73.77 ± 6.7%) at an intensity of 200 μg/ml, though it was 65.09 ± 2.5% and 57.99 ± 3.1% for PIP@ZIF-8 and PIP, respectively. In vivo studies on zebrafish showed that DOX administration remarkably impaired cognitive activity in T-Maze, and downregulated spatial memory and locomotor activity in the open field test. In addition, DOX administration caused a downregulation in GSH and SOD levels and increase in LPO, AChE and TNF-α levels compared to the vehicle group along with changes in brain histopathology. Further, PIP@ZIF-8 reversed the DOX-induced cognitive impairments by its antioxidant and neuroprotective properties. It can be concluded that PIP@ZIF-8 has a promising therapeutic potential against the chemotherapy-induced cognitive impairments in zebrafish. © 2023 Elsevier B.V.