Browsing by Author "Ashish Kumar Pandey"

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Biochemical and Molecular Evaluation of Rhizobium spp. and its Growth Promotion Studies with Lentil (Lens culinaris Medik. L.)
(Journal of Pure and Applied Microbiology, 2023) Umakant Banjare; Arun Kumar Patel; Ashish Kumar Pandey; Santosh Kumar; Ramesh Kumar Singh; Prahlad Masurkar; Rakesh Kumar Singh; Sandeep Kumar Gupta
Growth promoting Rhizobium spp are frequently used as biofertilizers for agricultural cropping system. Furthermore, Isolation, screening and biochemical characterization of Rhizobium for a specific plant is necessary to examine ability of isolated bacteria to affect the growth and development of host plant in various ways. The current study was aimed to isolate plant specific rhizobacterial strains which are compatible with lentil (Lens culinaris Medik.L.) plant. 20 bacterial isolates have been isolated from root nodules of lentil from various agro ecological area and their biochemical characterization was performed by different plant growth promotion activities. The result showed that, among 20 isolates, four isolates have vigorous plant growth promoting activities. Four bacterial strains were able to solubilise phosphorous along with hormone production. Moreover, among four bacterial strains, two strongly produced HCN and siderophore in vitro. Subsequently, all selected bacterial isolates were inoculated in lentil seeds of variety HUL57 to study germination percentage and vigour index of the crop. Out of four isolates 26N isolate performed best growth promotion activities on lentil seedlings. Finally, on the basis of performance of bacteria on plant, four isolates were characterized using molecular approach of species identification such as 16S rRNA sequencing. © The Author(s) 2023.
Characterization and identification of pigeonpea [cajanus cajan (L.) millsp.] genotypes based on quality of seed protein
(Agricultural Research Communication Centre, 2020) Ashish Kumar Pandey; Ajai Kumar Singh; Prakash Singh; Rajendra Prasad Vyas; Ravish Kumar Singh; Hemraj Bhandari
Tris-and water-soluble seed protein profiling was used to characterize, identify and differentiate 13 genotypes of pigeonpea via SDS– PAGE based electrophoresis of seed protein. Electropherograms, RM-value and UPGMA based cluster (dendrograms) analysis was used to analyze tris-and water-soluble protein banding patterns of these genotypes. It revealed that RM-value of protein bands have differed from all the genotypes for both soluble proteins. Tris-soluble protein banding pattern exhibited 17 bands including 12-15 variate of RM-value (0.083 to 0.98) and two common bands with RM-value 0.083 and 0.78. However, water-soluble protein banding pattern revealed the presence of 16 bands including 8-12 variate of RM-value (0.16 to 0.95) with four common bands of RM-value 0.43, 048, 0.23 and 0.65, respectively. UPGMA cluster analysis was used to group the 13 genotypes into six distinct clusters. The banding pattern of tris-soluble protein was found to be more distinct than water-soluble protein. The result demonstrated that the electrophoretic profile of tris-soluble protein through SDS-PAGE was more effective than the water-soluble protein. © 2020, Agricultural Research Communication Centre. All rights reserved.
Gene action analysis and identification of combiners for yield traits in maize
(Society for Plant Research, 2017) Ravish Kumar Singh; Erappa Gangappa; Prakash Singh; Ravi P. Singh; Ashish Kumar Pandey; Mukesh K. Singh
Field experiment was conducted using half diallel analysis involving 14 high yielding drought tolerant inbreds to generate 91 F1 hybrids of maize (Zea mays L.) to study the gene action and identify the best combiners for grain yield and its associated traits. The analysis of variances represents significant amounts of variability present among inbreeds for most of the traits studied. The estimates of general combining ability (GCA) and specific combining ability (SCA) variances were found significant, it indicated the preponderance of both additive and non-additive gene actions for the expression of yield controlling traits in maize. The parent CML-359 and CML-41 were found best general combiners, showing significant GCA effect for grain yield and its associated traits, and also simultaneously possessed high per se performance. However, the hybrids, CML-326 × DMR-N-21, HKI 26-24 (1-2) × CM-500, HKI-26-24(1-2) × CML-411, CML-41 × HKI-164-4(1-3)-2 and CML-41 × CM-500 were found best specific combiners for grain yield and its associated traits. The present study revealed that 5 best identified hybrids among 91 hybrids, may be used directly as hybrids for development of synthetics or composites or high yielding hybrids of maize (Zea mays L.). © 2017, SPR, All Rights Reserved.