Browsing by Author "Ashish Yadav"

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Effect of ultraviolet irradiation on the osseointegration of a titanium alloy with bone
(Medknow Publications, 2017) Ashish Yadav; Ranjana Yadav; Aratee Gupta; Akash Baranwal; Atul Bhatnagar; Vakil Singh
Introduction: Attempt has been made to analyze the potential of titanium (Ti) alloy for osteointegration by the effect of surface photo functionalization in different aspects as follows: in Ringer's solution, in vitro cell growth, and in vivo study on rabbit. The present study was aimed to investigate the influence of ultraviolet (UV) light on surface topography, corrosion behavior, and bioactivity of indigenously manufactured samples of Ti alloy mini-implant. Materials and Methods: The study includes surface modification of Ti samples by UV treatment, corrosion testing of the specimens using Potentiostat (GAMRY System), qualitative examination of modified surface topography using scanning electron microscope, and cellular viability test on Ti alloy surface (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide ASSAY). To find the effect of UV light on implant bone integration, biochemical test was performed on the femur of rabbits. Results and Discussion: Corrosion resistance of untreated Ti alloy in Ringer's solution was found to be less, whereas corrosion rate was more. Corrosion resistance of UV-Treated samples was found to increase significantly, thereby lowering the corrosion rate. Cell growth in UV-Treated specimen was observed to be higher than that in untreated samples. It is important to mention that cell growth was significantly enhanced on samples which were UV treated for longer duration of time. Conclusions: There was a marked improvement in cell growth on UV-Treated Ti alloy samples. Hence, it is expected that it would enhance the process of osseointegration of Ti with bone. Another important finding obtained was that the removal torque values of UV-Treated implants were higher than that of untreated implants. The overall result reveals that UV treatment of implants does help us in speeding up the osseointegration process. © 2017 Medknow Publications. All rights reserved.
Genetically modified fruit and vegetable - An overview on senescence regulation, postharvest nutraceutical quality preservation and shelf life extension
(Taylor and Francis Ltd., 2021) Ram Asrey; Kalyan Barman; Uma Prajapati; Swati Sharma; Ashish Yadav
Genetic engineering techniques offer opportunities for deliberate modulation and enhance the bio-functionality and bio-availability of horticultural crops, particularly fruit and vegetable which can aid in realising the goals of food and nutritional security for the burgeoning world population. The need of the hour calls upon us to use modern-day technologies such as genetic engineering in horticultural field as well for eradication of globally widespread hunger and malnutrition while the population is escalating and climate change is imminent. However, rigorous testing and trials to elucidate any unfounded fear and notion are warranted before the release of genetically modified (GM) crops for ensuring environmental and food safety. In this review, there is an attempt by the authors to compile and discuss the modulation in postharvest biology, edible quality, functional properties and storage life of genetically modified fruit and vegetable along with challenges in their use and consumer acceptance. The review also provides an outline of GM fruit and vegetable research prioritisation along with road map for future work. © 2020 The Journal of Horticultural Science & Biotechnology Trust.
Greulich and Pyle atlas: a non-reliable skeletal maturity assessment method in the North Indian population
(Springer, 2024) Praveen Kumar Tiwari; Amit Kumar Nayak; Ashish Verma; Surendra Kumar Pandey; Anand Mishra; Deepa Devadas; Ashish Yadav; Mayank Gupta
Forensic age assessments are crucial in the evaluation of criminal responsibility and preventing false age claims. Of all the methods available, the Greulich and Pyle (GP) atlas is most commonly used for age estimation purposes. Therefore, the current study sought to analyze the reliability and applicability of the GP standard and, additionally, to determine any possible association between the socioeconomic status (SES), food habits, and estimated skeletal maturity in the North Indian population. The study included 627 (334 males and 293 females) healthy children up to 19 years of age with varying SES and food habits. The skeletal age (SA) was estimated by three different evaluators using the GP atlas. The chronological mean age (CA) and SA were compared in different age cohorts. A paired t-test and a Pearson chi-square test were applied to show the difference between CA and estimated SA and the association of skeletal maturity with SES and food habits. The estimated skeletal age in males was retarded by 0.142 years or 1.72 months (p ≤ 0.05), whereas in females, it was retarded by 0.259 years or 3.12 months (p ≤ 0.05). In males, the GP method has significantly underestimated SA in age cohorts 3–4, 4–5, 6–7, 7–8, 8–9, and 12–13, whereas it overestimated in 10–11 and 18–19 years. However, in females, the SA was significantly underestimated in age groups 10–11, 12–13, and 14–15, respectively. Estimated skeletal maturity had no significant association with SES and food habits. The current study concludes that the GP atlas may not be applicable to North India’s population. The observed difference in assessed skeletal maturity may be due to geographical region, genetics, hormonal effects, etc., which require further investigation. Hence, population-specific standards are necessary to determine the bone age of Indian children accurately. © The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2023.
Immediate placement of implant in fresh extraction socket with early loading
(Wolters Kluwer Medknow Publications, 2012) Archana Singh; Aratee Gupta; Ashish Yadav; T.P. Chaturvedi; Atul Bhatnagar; B.P. Singh
Implant placement in maxillary anterior region has most aesthetic challenges in implant dentistry because tooth loss lead to bone resorption and collapse of gingival architecture, which lead to aesthetic compromise and inadequate bone for implant placement. Immediate implant placement into fresh extraction socket reduces the treatment time, cost, preserved the gingival aesthetic and increases the comfort of the patient. This article describes the procedure for immediate implant placement in fresh extraction socket and early loading of implant with zirconia crown. Clinical and radiographic examination revealed width and length of the tooth for selecting implant size and design. Cement retained zirconia crown was used for early loading. Implant was successfully loaded and was functional during 36 months follow up period. Immediate placement and early loading of dental implant provides advantages like fewer surgical procedures, shorter treatment time, and improved aesthetic and psychological confidence. © 2012 Wolters Kluwer Medknow Publications. All rights reserved.
In vitro studies on immunotoxic potential of Orange II in splenocytes
(2012) Ashish Yadav; Arvind Kumar; Premendra Dhar Dwivedi; Anurag Tripathi; Mukul Das
Orange II, an azo dye, is not permitted in food preparations, but high levels of the dye have been detected in different food commodities. Though there are reports on the toxicity of Orange II but knowledge based on the immunomodulatory properties of Orange II is scanty. The present investigation was undertaken to study the in vitro immunotoxic potential of Orange II in splenocytes. Splenocytes were isolated, cultured and subjected to immunophenotypic analysis, mixed lymphocyte reaction (MLR) assay or stimulated with lipopolysaccharide (LPS) or concanavalin A (Con A) for 72. h. The supernatant was collected for cytokine assays. Orange II showed cytotoxic effects at 100-1000 μg/ml concentrations and 50 μg/ml was determined as the highest non-cytotoxic dose. Orange II at the non-cytotoxic dose (50 μg/ml) significantly altered the relative distribution of T and B-cells, MLR response and the mitogen induced proliferative response of T-cells and B-cells. Consistent with the hypo-responsiveness of the T and B-lymphocytes, Orange II induced a concomitant decline in the secretion of cytokines IL-2, IL-4, IL-6, IFN-γ, TNF-α and IL-17. On the contrary, there was an increase in the production of IL-10, an anti-inflammatory regulatory cytokine, which may be one of the causative factor for immunosuppressive property of Orange II. These results suggest that non-cytotoxic dose of Orange II may have immunomodulatory effects. © 2011 Elsevier Ireland Ltd.
Management of fractured implant case using loop connector fixed partial denture
(2013) Ashish Yadav; Aratee Gupta; Amrit Tandan; Sulabh Kumar
Dental implants treatment have become a common modality of treatment nowadays for the replacement of missing tooth/teeth, but there have been failures due to a number of reasons; one of the rare problems is the fracture of the dental implants fixture. It is of prime importance to understand the type/system of the implant to be placed in a site and the amount and direction of occlusal forces incurred after the loading of prosthesis. Copyright 2013 BMJ Publishing Group. All rights reserved.
Phytochemical and antibacterial insights into crude pigment of endophytic fungi Corynespora torulosa (Syd.) Crous for its preliminary application for dyeing
(Taylor and Francis Ltd., 2025) Subham Singha; Prabha Toppo; Ashish Yadav; Debamalla Pathak; Piyush Mathur
The present work aimed to isolate pigment-producing endophytic fungi from the medicinal plant Hygrophila erecta. Sterilized plant tissue (leaf) was cultured on Potato Dextrose Agar media to isolate endophytic fungi. Morphological identification was complemented by molecular characterization using ITS region sequencing and it was identified as Corynespora torulosa (Syd.) Crous. Pigmentation was checked in different media and maximum production was noted in Czapek Dox Broth at pH 9.0 and 67.04 ± 1.38 mg of crude extract was quantified. Qualitative detection of secondary metabolites in crude pigment extract was carried out by standard procedures, interestingly which disclosed the presence of flavonoids, phenols, terpenoids, sterols and saponins. The antibacterial efficacy of the pigment was evaluated against a panel of pathogenic bacteria, including Escherichia coli ATCC 2592, Salmonella typhimurium ATCC 25241 and Bacillus subtilis ATCC 11774 using the agar well diffusion method giving inhibition zones of 12.31 ± 0.78 mm, 16.80 ± 0.54 mm and 23.88 ± 0.39 mm respectively. GC-MS analysis revealed several compounds with potent anti-microbial, anti-inflammatory and analgesic activities. Textile dyeing treatments of color fastness to washing were tested, and crude extracts of C. torulusa have provided promising results for dyeing and mordanting. © 2025 Har Krishan Bhalla & Sons.
Reference Range of Thyroid Hormones in Pregnancy: Customization Needed for Pregnant Women in India
(Springer, 2025) Nalini Arora; Pallavi Kashyap; Dipankar Saren; Priyanka Meel; Joya Ghosh; Ashish Yadav
Background: The precise interpretation of thyroid function tests during pregnancy needs population-based trimester-specific data among pregnant women. This study was to determine trimester-specific reference range for free triiodothyronine (FT3), free thyroxine (FT4) and thyrotropin (TSH) among pregnant women in India. Methods: In this prospective observational study, asymptomatic pregnant women with single fetus were enrolled from each trimester. Serum FT3, FT4, TSH and anti-thyroperoxidase (anti-TPO) antibodies were estimated using electrochemiluminescence technique. Results: Among 918 pregnant women, 82 women were excluded due to the presence of anti-TPO antibodies (76) and overt hypothyroidism (6). Among the remaining 836 women, 279 (33.3%) were in first trimester, 309 (36.9%) in second and 248 (29.6%) in third trimester. The 5th and 95th percentile values for each trimester were used as reference ranges. For all three hormones, the reference ranges for the first, second and third trimesters were: FT3 (1.59–3.64, 1.60–3.50 and 1.44–3.28 pg/dl), FT4 (0.64–1.12, 0.64–1.05 and 0.60–1.01 ng/dl) and TSH (0.21–4.95, 0.23–4.90 and 0.14–4.59 µIU/ml). The mean and median values for TSH between each trimester showed no statistically significant difference. No specific trend was seen for FT3 and TSH with advancing gestation. FT4 showed a decreasing trend with advancing trimester (P value: first versus second = 0.01, first versus third = 0.00003 and second versus third = 0.004). The reference range (N = 836) irrespective of trimester for thyroid hormones was: FT3 (1.53–3.46 pg/dl), FT4 (0.63–1.08 ng/dl) and TSH (0.19–4.72 µIU/ml). Conclusion: The trimester-specific reference levels of TSH among pregnant women from India are significantly higher than 4 mIU/L (American Thyroid Association 2017). © The Author(s) 2025.
Sodium benzoate, a food preservative, affects the functional and activation status of splenocytes at non cytotoxic dose
(Elsevier Ltd, 2016) Ashish Yadav; Arvind Kumar; Mukul Das; Anurag Tripathi
Sodium benzoate (SB) is a widely used food preservative due to its bacteriostatic and fungistatic properties. The acceptable daily intake of SB is 5 mg/kg-bw, however, it has been found to be used in the food commodities at relatively high levels (2119 mg/kg). Earlier studies on SB have shown its immunosuppressive properties, but comprehensive immunotoxicity data is lacking. Our studies have shown that SB was non cytotoxic in splenocytes up to 1000 μg/ml for 72 h, however at 2500 μg/ml it was found to be cytotoxic. Thus, 1000 μg/ml dose of SB was chosen for the subsequent experiments. SB significantly suppresses the proliferation of Con A and LPS stimulated splenocytes at 72 h, while allogenic response of T cells was significantly decreased after 96 h. SB did not affect the relative expression of CD3e or CD4 molecules following 72 h exposure, however, it downregulated the relative expression of CD8 co-receptor. Further, exposure of splenocytes to SB for 72 h led to reduced expression of CD28 and CD95, which play a vital role in T cell activation. SB also suppresses the relative expression of CD19, CD40 and CD95 receptors on B cells after 72 h. In addition to the functional responses, SB lowered the expression of IL4, IL6, IFNγ and IL17 cytokines in Con A stimulated splenocytes; and IL6, IFNγ and TNFα in LPS stimulated splenocytes following 48 h of exposure. Taken together, the present study is suggestive of the immunomodulatory potential of SB. © 2015.
Sunset yellow FCF, a permitted food dye, alters functional responses of splenocytes at non-cytotoxic dose
(2013) Ashish Yadav; Arvind Kumar; Anurag Tripathi; Mukul Das
Sunset yellow FCF (SY), a permitted food color, is extensively used in various food preparations and quite often exceeds the permissible levels (100-200. mg/kg). Several toxicity studies on SY are reported, however immunomodulatory properties have not been explored yet. To investigate the immunotoxic properties of SY, splenocytes were isolated, cultured and subjected to mitogen stimulated proliferation assay (lipopolysaccharide, LPS or concanavalin A, Con A), mixed lymphocyte reaction (MLR) assay, immunophenotypic analysis of cell surface receptor expression and assay for cytokines release in the culture supernatants were performed in the presence of SY. Since SY did not exhibit any cytotoxicity up to 250 μg/ml, this dose was used for further studies. It was observed that SY (250 μg/ml) significantly (p<. 0.05) suppressed the mitogen induced proliferation of splenocytes and MLR response. Further, immunophenotypic analysis revealed that SY alters the relative expression of CD3e/CD4/CD8 in T cells and CD19 in B-cells. Consistent with the suppression of T-cell and B-cell responses and altered surface receptor expression, SY also lowered the expression of IL2, IL4, IL6, IL-17, IFN-γ and TNF-α cytokines. These results suggest that non-cytotoxic dose of SY may have immunomodulatory effects. © 2012 Elsevier Ireland Ltd.