Browsing by Author "Ashok Kumar Gupta"

Now showing 1 - 6 of 6

Antidiabetic activity and molecular docking of fructooligosaccharides produced by Aureobasidium pullulans in poloxamer-407-induced T2DM rats
(2013) Sudhanshu Kumar Bharti; Supriya Krishnan; Amit Kumar; Kaushal Kishore Rajak; Krishna Murari; Binod Kumar Bharti; Ashok Kumar Gupta
This study evaluated the beneficial effects of fructooligosaccharide (FOS) intake from Aureobasidium pullulans using poloxamer-407 (PX-407) induced type 2 diabetes mellitus (T2DM) in rat. Administration of FOS enhanced enzymatic activities of catalase and glutathione reductase in a dose-dependent manner. Significant reduction in fasting plasma triacylglycerol and very low-density lipoprotein level coupled with slight increase in fasting plasma insulin level was observed. Significant decrease in severe glucosuria, proteinuria, blood creatinine, urea and advanced glycation end products was also observed. Supplementation of FOS increased glucagon like peptide-1 content as well as Bifidobacteria and Lactobacilli populations in the caecum. Molecular docking by Gold and Glide software revealed that three sugar types present in the FOS (1-kestose, nystose, and 1-β-fructofuranosyl nystose) are potent dipeptidyl peptidase-IV inhibitors as well as peroxisome proliferator-activated receptor-gamma agonists. This work indicates that FOS can be positioned as a nutraceutical product, beneficial in diabetes-associated metabolic abnormalities. © 2012 Elsevier Ltd. All rights reserved.
Citric acid-assisted inexpensive semi-wet combustion synthesis and characterization of ultrafine LiFeinf0.95/infTiinf0.05/infPOinf4/inf and LiFePOinf4/inf polycrystalline materials
(Pan Stanford Publishing Pte. Ltd., 2022) Laxman Singh; Dev Kumar Mahato; R.N. Rai; Ashok Kumar Gupta; Youngil Lee
[No abstract available]
Effectiveness of conventional drug therapy of plaque psoriasis in the context of consensus guidelines: A prospective observational study in 150 patients
(2013) Ashok Kumar Gupta; Shyam Sunder Pandey; Bajrangprasad Laxminarayan Pandey
Background: Evidence for superior outcome by adhering to therapy guidelines is imperative to their acceptance and adaptation for the optimal management of disease variants. Objective: Comparative study of prospective outcomes in simultaneous consideration of independent variables in groups of 150 patients of plaque psoriasis either treated adhering to or in digression of standard guidelines. Methods: The psoriasis area severity index (PASI) and the dermatology life quality index (DLQI), prior to and after three months of uninterrupted therapy were examined in treatment groups among 150 patients. Recovery rates of 75% or more in PASI were compared. Independent variables were also examined for their bearing on the outcome. Results: The vast majority was early onset disease phenotype. All three treatment regimens when administered in adherence to the guidelines yielded significantly superior rates of defined recovery both in PASI and DLQI. Compromise of the therapeutic outcome appeared in high stress profiles, obesity, female sex and alcohol, tobacco or smoking habit. Conclusion: Conventional drug therapy of plaque psoriasis yields superior outcome by adhering to the consensus guidelines. Psychiatric address to stress must be integral and special considerations for phenotypic/syndromic variants is emphasized for effective therapy of psoriasis. Copyright © 2008 The Korean Dermatological Association.
Essential oil of cymbopogon citratus against diabetes: Validation by In vivo experiments and computational studies
(2013) Sudhanshu Kumar Bharti; Amit Kumar; Om Prakash; Supriya Krishnan; Ashok Kumar Gupta
This study evaluates the antidiabetic activities of essential oil obtained by steam distillation of the leaf sheath of Cymbopogon citratus (CCEO) in poloxamer-407 induced type 2 diabetic (T2D) Wistar rats. The sample was then analyzed by gas chromatography-mass spectroscopy (GCMS) identifying 23 compounds representing 96.9% of the oil. The major compounds of essential oil were geranial (42.4%), neral (29.8%), myrcene (8.9%) and geraniol (8.5%). When compared to diabetic control rats, the CCEO treated diabetic rats presented significant amelioration of glycaemia, insulinamia and lipid dysmetabolism, accompanied by increased GLP-1 content in cecum and remarkable reduction of oxidative markers. Histopatholgical analysis of pancreas showed increase in β-cell mass, islet number and quality of insulitis. HYBRID and FRED docking were performed for 48 documented CCEO phytoconstituents for putative action mechanism concerning three proteins namely PTP-1B, PPAR-γ and DPP IV having diabetic therapeutic properties. Phytoconstituents like myrcenol, linalool, α-elemol and β Eudesmol showed significant interaction with PPAR-γ and DPP-IV while only pimelyl dihydrazide showed interaction with PTP-1B. The results provided a pharmacological evidence of CCEO as antidiabetic mediated by interaction of various phytoconstituents with multiple targets operating in diabetes mellitus. © 2013 Bharti SK, et al.
Superconductivity in nontransition metals. I
(1969) J.S. Rajput; Ashok Kumar Gupta
The theoretical calculation of superconducting-state parameters Vph, VC, and Z0 [Vph estimates the phonon-mediated attractive electron-electron interaction in the metal; VC, the Coulomb pseudo-potential, measures the effectiveness of the Coulomb repulsion in inhibiting superconductivity; and Z0, the quasiparticle mass (or energy) renormalization parameter, takes into account the many-body renormalization effects due to electron-phonon and Coulomb interactions] is undertaken for both nonsuperconductors and simple-metal (nontransition-metal) superconductors. The two questions faced are: why non-superconductors (alkali, alkaline-earth, and noble metals) are nonsuperconductors (this question arises from the startling prediction by Morel and Anderson that all metals should be superconductors); and whether better agreement can be achieved between theoretically estimated superconducting-state parameters and empirical parameters better, e.g., than those of Pines and of Morel and Anderson. Our calculation is differentiated from that of Morel and Anderson first in using electron-phonon and Coulomb-interaction matrix elements between Bloch electron states, secondly in using for the electron-phonon matrix element the form suggested by Harrison for the orthogonalized-plane-wave form factor, and lastly in including the renormalization effects due to electron-phonon and Coulomb interactions. The renormalization due to Coulomb interaction is included by taking the values from the calculations by Rice. The Bloch electron states are calculated in a model in which the ionic core is replaced by a three-dimensional constant repulsive potential, and the wave function is determined in the spirit of the Wigner-Seitz approximation and first-order perturbation theory. The theoretically estimated parameters Vph, VC, and Z0 are compared with the empirically estimated parameters. These are also compared with the parameters estimated by Garland. A good comparison better than that of Morel and Anderson is found between theoretical and empirical parameters. It is pointed out that if renormalization effects are included in the calculation by Morel and Anderson, then their apparently good comparison with empirical parameters does not remain so good. In the case of nonsuperconductors, we find that the phonon-mediated attractive interaction fails to dominate over the Coulomb repulsion, contrary to the conclusions of Morel and Anderson, and the effective interaction strength (Vph-VC)Z0, which is analogous to the BCS parameter N0V, is repulsive or very slightly attractive. The nonsuperconducting status is thus restored to alkali and alkaline-earth metals. © 1969 The American Physical Society.
Tocopherol from seeds of Cucurbita pepo against diabetes: Validation by invivo experiments supported by computational docking
(2013) Sudhanshu Kumar Bharti; Amit Kumar; Neeraj Kumar Sharma; Om Prakash; Sudhir Kumar Jaiswal; Supriya Krishnan; Ashok Kumar Gupta; Awanish Kumar
Background/purpose: Tocopherol from raw pumpkin seeds has been reported to be effective in the alleviation of diabetes through its antioxidant activities. This study evaluates the antidiabetic activities of the tocopherol fraction of raw seeds of Cucurbita pepo L. (CPSE) in a diabetic rat model. In addition, the putative action mechanisms of its botanicals were computationally investigated. Methods: Seed water activity (Aw) was assessed. Tocopherol was extracted and quantified from raw seed oil. The effect of CPSE was studied in poloxamer-407 (PX-407)-induced type 2 diabetic Wistar rats. Glycemic, insulinemic, and lipid profiles, as well as lipid peroxidation status, were evaluated. Glucagon like peptide-1 (GLP-1) content in the cecum was evaluated and histopathological analysis of the pancreas was performed. Further, HYBRID and FRED docking were performed for 10 documented CPSE botanicals, for putative action mechanisms concerning three proteins [protein-tyrosine phosphatase 1B (PTP-1B), peroxisome proliferator-activated receptor gamma (PPAR-γ), and dipeptidyl peptidase IV (DPP-IV)] known to have diabetic therapeutic potential. Results: The Aw of raw seeds was found to be 0.544±0.002. Using tocopherol standards, HPLC determination of CPSE revealed the presence of tocopherol isomers (α, β, γ, and δ). The tocopherol content was found to be 107.4±2.9mg/100g of CPSE. When compared to diabetic control (DC) rats, the CPSE-treated diabetic rats presented a significant amelioration of glycemia, insulinemia, and lipid dysmetabolism. A remarkable reduction in oxidative markers and improved cecal and pancreatic characteristics were also observed. Tocopherol isomers have shown a considerable interaction potential with the aforesaid proteins in docking. Conclusion: The results provide pharmacological evidence of CPSE as an antihyperglycemic mediated by the interaction of various botanicals with multiple targets operating in diabetes mellitus (DM). © 2013.