Browsing by Author "Ashutosh Kumar Dubey"

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Development of luminescent atacamite nanoclusters for bioimaging and photothermal applications
(Institute of Physics Publishing, 2020) Kedar Sahoo; Deepak Khare; Saripella Srikrishna; Ashutosh Kumar Dubey; Manoj Kumar
Fluorescent atacamite nanoclusters (FANCs) have been developed and modified with silica for Drosophila salivary gland tissue imaging and photothermally induced cell death of osteosarcoma MG-63 cells. FANCs were synthesized with Moringa oleifera leaf extract without using any hazardous reducing and external capping agents. FANC was further used to evaluate light absorption, fluorescence emission, band gap, and magnetic properties as the first report on such nanoclusters. Upon excitation with a 350 nm light source, FANCs exhibited fluorescence at 460 nm, with a relative quantum yield of 0.3%. Besides, silica-encapsulated fluorescent atacamite nanoclusters (SEFANC) manifested remarkable improvement in emission, quantum yield (1.7%), shelf-life (15 d), biocompatibility, and photostability. Concomitantly, it has also increased the absorption in the near-infrared region and demonstrated high heat generation potential (42 C → 50 C). The above results suggest that FANC can be a potential candidate in the area of nanomedicine for a number of applications such as bioimaging, photothermal therapy, etc. © 2020 IOP Publishing Ltd.
Effect of carbon quantum dots derived from extracts of UV-B-exposed Eclipta alba on alcohol-induced liver cirrhosis in Golden Hamster
(Springer Nature, 2023) Kshama Rai; Kanchan Yadav; Megha Das; Shilpi Chaudhary; Kaustubh Naik; Priya Singh; Ashutosh Kumar Dubey; Sanjeev Kumar Yadav; Shashi Bhushan Agrawal; Avanish Singh Parmar
The Eclipta alba plant is considered hepatoprotective, owing to its phytoconstituents wedelolactone. In the current study, effect of elevated ultraviolet-B (eUV-B) radiation was investigated on biochemical, phytochemical, and antioxidative enzymatic activities of E. alba (Bhringraj) plant. The UV-B exposure resulted in an increase in oxidative stress, which has caused an imbalance in phytochemical, biochemical constituents, and induced antioxidative enzymatic activities. It was observed that the UV-B exposure promoted wedelolactone yield by 23.64%. Further, the leaf extract of UV-B-exposed plants was used for the synthesis of carbon quantum dots (CQDs) using low cost, one-step hydrothermal technique and its biocompatibility was studied using in vitro MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay on HepG2 liver cell line. It revealed no toxicity in any treatment groups in comparison to the control. Both CQDs and leaf extract were orally administered to the golden hamster suffering from alcohol-induced liver cirrhosis. In the morphometric study, it was clearly observed that a combination of UV-B-exposed leaf extract and synthesized CQDs delivered the best result with maximum recovery of liver tissues. The present study reveals the positive impact of UV-B exposure on the medicinally important plant, increased yield of wedelolactone, and its enhanced hepatoprotective efficacy for the treatment of damaged liver tissues. Graphical Abstract: [Figure not available: see fulltext.]. © 2023, The Author(s), under exclusive licence to European Photochemistry Association, European Society for Photobiology.
Electret-induced antibacterial response of Mg1-xCaxSi1-xZrxO3 (x = 0–0.4) bioceramics
(John Wiley and Sons Inc, 2024) Priya Singh; Ashutosh Kumar Dubey
The potential risk of bacteria-induced prosthetic infection during implantation/post-operative healing raises a serious concern about the success of implant/surgery. In this context, the present study successfully established the antibacterial efficacy of the electret form of Mg1-xCaxSi1-xZrxO3 (x = 0, 0.1, 0.2, 0.3, 0.4 [MCSZO-X]) bioceramics, toward both, Escherichia Coli and Staphylococcus aureus bacteria with reference to those of hydroxyapatite (HAP). MCSZO-X bioceramics were synthesized using a solid-state route. The influence of Ca- and Zr co-doping on the crystallite size of MCSZO-X has been analyzed using X-ray peak profile analyses. The electrets were developed by corona poling of sintered MCSZO-X samples at voltage and temperature of 20 kV and 500°C (30 min), respectively. The charge, stored on the surface of MCSZO-X electret samples was calculated to be 0.253, 0.294, 0.320, 0.173, and 0.161 µC/cm2 via thermally stimulated depolarized current measurement. The positive and negative ends of MCSZO- X electrets exhibited a reduction in the viability of E. coli bacteria by (24%, 25%, 30%, 43%, and 42%) and (29%, 37%, 39%, 51%, and 48%), respectively, in comparison to those of HAP. On the contrary, the viability of S. aureus bacteria has decreased by (26%, 33%, 35%, 46%, and 42%) and (21%, 22%, 27%, 395, and 37%) on the surfaces of the positive and negative ends of MCSZO- X electrets, respectively, with the reference of those of HAP. The mechanism of electret-induced antibacterial activity has been revealed via various assays, such as catalase activity, superoxide production (SOD), protein estimation, and lipid peroxidation (LPO) assays. The positive ends of MCSZO-X electrets demonstrate antibacterial efficacy by means of more reactive oxygen species generation as compared to their negative ends of electrets and uncharged surfaces of MCSZO-X samples, as revealed by SOD, protein estimation, catalase activity, and LPO assays. However, the negative ends of electrets prevent the adhesion of bacterial cells via electrostatic repulsion. © 2024 The American Ceramic Society.
Piezoelectric catalyst BaTiO3 and K0.5Na0.5NbO3 induced cellular and antibacterial response in poly (vinylidene fluoride) for self-powered implants for orthopedic applications
(Elsevier B.V., 2025) Kuntal Kumar das; Urvashi Kesarwani; Ravi Prakash; Pralay Maiti; Om Shankar; Ashutosh Kumar Dubey
Despite of excellent biocompatibility of piezoelectric poly(vinylidene fluoride) (PVDF), lower resistance towards bacteria and piezoelectric coefficient restricts it's widespread application as self-powered implant. Towards this end, this study investigates the effect of incorporation of piezo-catalysts BaTiO3 (BT) and K0.5Na0.5NbO3 (KNN) into PVDF on piezo-catalytic characteristics, cellular and antibacterial response. Thin films of PVDF and its composites were prepared using the solvent casting method and hot compression molding. The piezo-catalytic response is observed to be notably higher in the composites than that of pure PVDF. The measured values of voltage, and power for PVDF, PVDF-10 wt% BT (PVDF-10BT) and PVDF-10 wt% KNN (PVDF-10KNN) are (24 V, ∼4.4 µW/cm2), (92 V, ∼15.8 µW/cm2) and (103 V, ∼19.4 µW/cm2), respectively. The presence of piezo-catalyst BT and KNN significantly enhance the cell proliferation, osteogenic differentiation (ALP) of MG-63 osteoblast-like cells and hemocompatibility. Incorporation of piezo-catalysts is observed to improve significantly the antibacterial response against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) due to a higher production of reactive oxygen species. These results suggest that PVDF modified with BaTiO3 and K0.5Na0.5NbO3 piezo-catalysts are promising candidates as self-powered implants for orthopedic applications. © 2025 Elsevier B.V.
Role of Piezoelectricity in Disease Diagnosis and Treatment: A Review
(American Chemical Society, 2024) Pratishtha Tripathi; Ashutosh Kumar Dubey
Because of their unique electromechanical coupling response, piezoelectric smart biomaterials demonstrated distinctive capability toward effective, efficient, and quick diagnosis and treatment of a wide range of diseases. Such materials have potentiality to be utilized as wireless therapeutic methods with ultrasonic stimulation, which can be used as self-powered biomedical devices. An emerging advancement in the realm of personalized healthcare involves the utilization of piezoelectric biosensors for a range of therapeutic diagnosis such as diverse physiological signals in the human body, viruses, pathogens, and diseases like neurodegenerative ones, cancer, etc. The combination of piezoelectric nanoparticles with ultrasound has been established as a promising approach in sonodynamic therapy and piezocatalytic therapeutics and provides appealing alternatives for noninvasive treatments for cancer, chronic wounds, neurological diseases, etc. Innovations in implantable medical devices (IMDs), such as implantable piezoelectric energy generator (iPEG), offer significant advantages in improving physiological functioning and ability to power a cardiac pacemaker and restore the heart function. This comprehensive review critically evaluates the role of piezoelectricity in disease diagnosis and treatment, highlighting the implication of piezoelectric smart biomaterials for biomedical devices. It also discusses the potential of piezoelectric materials in healthcare monitoring, tissue engineering, and other medical applications while emphasizing future trends and challenges in the field. © 2024 American Chemical Society.
Sandalwood-derived carbon quantum dots as bioimaging tools to investigate the toxicological effects of malachite green in model organisms
(Elsevier Ltd, 2020) Devyani Shukla; Megha Das; Dipanshu Kasade; Maneesha Pandey; Ashutosh Kumar Dubey; Sanjeev Kumar Yadav; Avanish Singh Parmar
Malachite green is an N-methylated diaminophenylmethane dye that has generated much concern over its suggestive carcinogenic nature. After its excessive use in aquaculture industry as an effective ectoparasitide, much debate was raised over its toxicological effects leading to scientific studies conducted on animal models. Even after several bans, malachite green is still easily available in many parts of the world and unscrupulously even used to give green vegetables a fresher look. This study aims to address this concern by systematically studying the toxicological effects of malachite green through bioimaging in plant and animal cell and tissue. Sandalwood-derived carbon quantum dots have been used as a bioimaging tool since they are non-cytotoxic and show excellent fluorescence properties. Onion tissues demonstrate the translocation of the dye inside cells having high affinity for the nuclei and cell walls. Toxicological effects on the growth of Vigna radiata (mung beans) have been studied methodically. Bioimaging of the transverse cross-section of the dye-treated plant root shows a significant difference from the control. In animal cells, dose-dependent decrease in cell viability of MG-63 cells was observed with MG. CQD showed good fluorescence in both cytoplasm and nucleus of MG63 cells. In addition, CQDs were employed as a great tool for bioimaging of the histopathologically adverse effects of MG in Golden hamster animal model. This study showed CQDs could be used as an alternative non-site specific fluorescent probe for cell and tissue imaging for better visualization of cell and tissue architectural changes. © 2020 Elsevier Ltd
Synthesis and characterization of novel protein nanodots as drug delivery carriers with an enhanced biological efficacy of melatonin in breast cancer cells
(Royal Society of Chemistry, 2021) Kanchan Yadav; Megha Das; Nurul Hassan; Archana Mishra; Jayeeta Lahiri; Ashutosh Kumar Dubey; Sanjeev Kumar Yadav; Avanish Singh Parmar
Melatonin is a potent antioxidant, chemotherapeutic and chemo preventive agent against breast cancer. However, its short half-life is one of the major limitations in its application as a therapeutic drug. To overcome this issue, the green-emitting protein nanodot (PND) was synthesized by a one-step hydrothermal method for loading melatonin. The synthesized pH-7 and pH-2 PND showed a quantum yield of 22.1% and 14.0%, respectively. The physicochemical characterization of both PNDs showed similar morphological and functional activities. Furthermore, the biological efficacy of melatonin-loaded PND (MPND) was evaluated in a breast cancer cell line (MDA-MB-231) for live-cell imaging and enhanced nano-drug delivery efficacy. Interestingly, the permeability of neutral pH PND in both cell cytoplasm and nucleus nullifies the limitations of real-time live-cell imaging, and ensures nuclear drug delivery efficacy. Neutral pH PND showed better cell viability and cytotoxicity as a fluorescence bioimaging probe compared to acidic PND. The bioavailability and cell cytotoxicity effect of MPND on MDA-MB-231 breast cancer cells were studied through confocal and migration assay. Results showed that MPND causes enhanced bioavailability, better cellular uptake, and inhibition of the migration of breast cancer cells as compared to the drug alone. Besides, the synthesized MPND showed no sign of fluorescence quenching even at a high concentration of melatonin, making it an ideal nanocarrier for bioimaging and drug delivery. © The Royal Society of Chemistry 2021.
Tissue-engineered vascular grafts for cardiovascular disease management: Current strategies, challenges, and future perspectives
(John Wiley and Sons Inc, 2024) Kuntal Kumar Das; Ruchi Mishra Tiwari; Om Shankar; Pralay Maiti; Ashutosh Kumar Dubey
Cardiovascular diseases are the leading cause of mortality which primarily occurs due to the blood vessel obstruction or narrowing. Surgical procedures such as, coronary artery and peripheral artery bypass grafting frequently require vascular grafts for long-term revascularization. However, using autogenous vessels, such as the internal thoracic artery and saphenous vein, especially for vessels with diameters less than 6 mm, are associated with number of concerns due to limited availability, invasive retrieval procedures, and aptness. To overcome these limitations, the development of tissue-engineered vascular grafts (TEVGs) is in continuous thrust. This review comprehensively provides the potentiality of a range of artificial and naturally occurring biopolymers and their fabrication techniques, cell sources and seeding techniques to realize the state-of-the-art TEVGs. Moreover, this review article presents a synopsis of insights obtained from a variety of in vitro and in vivo studies, including human clinical trials. It underscores the need for further exploration into key areas such as optimal cell sources, seeding techniques, mechanical properties, hemodynamics, graft integration, the impact of patient conditions, optimum burst pressure, sufficient suture strength, hydrophilicity, biodegradability, and related factors. In summary, the review offers insights into the current strategies, challenges, and future perspectives of TEVG. © 2024 The Author(s). MedComm – Biomaterials and Applications published by John Wiley & Sons Australia, Ltd on behalf of Sichuan International Medical Exchange & Promotion Association (SCIMEA).