Browsing by Author "Avijit Ghosh"

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A 1,8 naphthalimide anchor rhodamine B based FRET probe for ratiometric detection of Cr3+ion in living cells
(Elsevier B.V., 2019) Susanta Adhikari; Sabyasachi Ta; Avijit Ghosh; Subhajit Guria; Abhishek Pal; Manisha Ahir; Arghya Adhikary; Sumit Kumar Hira; Partha Pratim Manna; Debasis Das
A 2-(1,3-dioxo-1H-benzo[de]isoquinolin-2(3H)-yl)acetaldehydeanchored rhodamine B based probe, RDNAP detects Cr3+ ion by fluorescence resonance energy transfer (FRET) process in aqueous buffered acetonitrile media (7:3, v/v). Conversely, conjugation of 2-(1,3-dioxo-6-(piperidin-1-yl)-1H-benzo[de]isoquinolin-2(3H)-yl)acetaldehyde with rhodamine B provides another probe, RDNAP-PY that undergoes Cr3+assisted ratiometric fluorescence and colorimetric change in the same media. RDNAP-PY provides higher FRET efficiency and detects as low as 1.81×10−6 M Cr3+with an association constant, 15.9 × 104 M−1. Other common ions do not interfere. RDNAP-PY efficiently images intracellular Cr3+ in live Hep3B, MCF-7, HeLa, SiHa and HEK 293T cells under fluorescence microscope in a ratiometric and time dependent manner. 1H NMR titration and DFT studies strongly support experimental findings. © 2018 Elsevier B.V.
Development of Controlled Release Fertilizer from Double-Boiled Linseed and Mustard Oil-Based Formulations: Surface Morphology, Nutrient Release and Performance of Wheat in Sub-tropical Inceptisol
(Springer Science and Business Media Deutschland GmbH, 2024) Abhijit Sarkar; Dipak Ranjan Biswas; Madhumonti Saha; Rajesh Kumar; B.S. Dwivedi; K.K. Bandyopadhyay; Ranjan Bhattacharyya; Siddhartha Sankar Biswas; Avijit Ghosh; Trisha Roy; Bappa Das; Abir Dey; Khurshid Alam; Sayon Mukherjee
Synchronization of nutrient release from fertilizers and plant nutrient demand could optimize the nutrient use efficiency and crop productivity, particularly for nitrogen (N) and phosphorus (P). N and P both have low nutrient use efficiency, while P has a use efficiency of only 15–20%. Four oil formulations (Oil-1, Oil-2, Oil-3 and Oil-4) were prepared from double-boiled linseed oil (DBLO) and mustard oil (MO) at different mass proportions. Oil formulations were characterized physically by observing surface wrinkle formation using Otsu’s algorithm for binary threshold images. Commercial di-ammonium phosphate (DAP) was coated with prepared oil formulations at 0, 4 and 8% (w/w) and assessed through scanning electron microscopy (SEM), while their nutrient release pattern in soil was studied at different temperatures (20 and 30°C) regimes. Finally net house pot culture experiment was conducted with wheat (Triticum aestivum L.) at different P doses (0, 25 and 50 mg P kg−1 soil) and P sources (uncoated DAP, 4%-Oil-1-DAP, 8%-Oil-1-DAP) with/without Aspergillus awamori. The binary threshold image directed Oil-1 had the lowest surface wrinkle, and SEM images also depicted Oil-1 coated DAP which had smooth surface morphology. Soil incubation data indicated that N is more temperature sensitive than P, and surface coating with oil formulation could extend the period of nutrient release. During soil incubation of oil formulation-coated DAP, N and P release was comparatively slow at higher coating levels. In pot culture experiment, the wheat grain, straw yield, relative chlorophyll, grain protein, soil mineral N, available P, inorganic P and dehydrogenase activity were significantly improved with the increased doses of P and A. awamori inoculation; however, microbial inoculation had non-significant effects on crop and soil parameters. Except post-harvest soil organic P, other parameters were strongly and positively correlated with grain yield of wheat. Coating with hydrophobic oil formulation could extend the nutrient release period with minimal intervention. In terms of crop and soil parameters, both 4%-Oil-1-DAP and 8%-Oil-1-DAP treated pots performed better than the uncoated DAP, but 4%-Oil-1-DAP could be considered as best for annual crops like wheat. For perennial crops, higher coating level could be more beneficial. © The Author(s) under exclusive licence to Sociedad Chilena de la Ciencia del Suelo 2024.
Impact of Soil Acidity Influenced by Long-term Integrated Use of Enriched Compost, Biofertilizers, and Fertilizer on Soil Microbial Activity and Biomass in Rice Under Acidic Soil
(Springer Science and Business Media Deutschland GmbH, 2021) Abhik Patra; Vinod Kumar Sharma; Dhruba Jyoti Nath; Avijit Ghosh; Tapan Jyoti Purakayastha; Mandira Barman; Sarvendra Kumar; Kapil Atmaram Chobhe; Ajin S. Anil; Ravindra Kumar Rekwar
To elucidate the effects of soil acidity influenced by 10 years of application of enriched compost (ECM), biofertilizers, and chemical fertilizers in integration on soil microbial activity and biomass in rice crop under acidic condition of soil. A field experiment was performed with five treatments, namely, T1, absolute control; T2, 100% recommended doses of nitrogen (N), phosphorus (P), and potassium (K); T3, 50% NP + 100% K + biofertilizers; T4, 50% NP + 100% K + 1 t ECM ha−1; and T5, 25% NP + 100% K + 2 t ECM ha−1, in a randomized block design. And each treatment was repeated four times. Experimental findings revealed that pH-dependent acidity, exchangeable acidity, and total potential acidity increased with soil depth; however, the application of ECM effectively controlled various forms of soil acidity by 31, 31, and 35%, respectively, in surface soil layer (0–5 cm). Soil acidity is greatly influenced by nutrient management practices; thus, the ECM application recorded significantly higher soil microbial biomass and soil functional diversity within all layers of soil over the 100% recommended dose of fertilizer (RDF) (T2). In comparison to 100% RDF, integrated nutrient management (INM) practices enhanced the microbial activities, viz., β-glucosidase, β-galactosidases, β-glucosaminidase, phenol oxidase, and peroxidase activities, by 18, 19, 20, 21, and 8%, respectively, at the surface soil layer. The use of ECM and chemical fertilizers in integration increases enzymatic activity and microbial density in the soil by controlling various forms of soil acidity. © 2021, Sociedad Chilena de la Ciencia del Suelo.
Novel bio-filtration method for the removal of heavy metals from municipal solid waste
(Elsevier B.V., 2020) M.C. Manna; Asha Sahu; Nirmal De; J.K. Thakur; Asit Mandal; Sudeshna Bhattacharjya; Avijit Ghosh; Mohammad Mahmudur Rahman; Ravi Naidu; Udai Bhan Singh; Raja Dakhli; M.P. Sharma; Sukanya Misra
Fast urbanization, and agricultural escalation produce a lot of municipal solid wastes (MSW). There is need to recognize economically practical innovations to debase substantial metals to an innocuous state. The present investigation aimed to recognize proficient fungi and develop bio-filtration strategy utilizing them for expulsion of substantial heavy metals (HMs) from tainted MSW compost (MSWC). Six fungi were isolated from sewage sludge, namely, Trichoderma viride, Aspergillus heteromorphus, Rhizomucor pusillus, Aspergillus flavus, Aspergillus terreus, and Aspergillus awamori. Growths of all these fungi were highly susceptible to Cd at 5 ppm. Trichoderma viride, Aspergillus awamori, and Rhizomucor pusillus were highly susceptible to Cu at 50 ppm. Aspergillus flavus and Aspergillus heteromorphus could tolerate Cr below 50 ppm. The varying degree of HM tolerance by different fungi may be due to diverse strategies to dodge HM toxicity. Functional groups like amide (-NH2), hydroxyl (-OH), carboxylate anions (-COO), carbonyl groups (-CO), C-F and C-Br were responsible for bio-sorption of heavy metals by fungi through chelation with HMs. We found Trichoderma viride and Aspergillus flavus based bio-filters were capable to remove the highest amount of Pb (>40%) and Cd (>20%). Aspergillus heteromorphus based bio-filters was the most efficient to remove Cu and Cr from MSWC (∼20%). Trichoderma viride, Aspergillus heteromorphus, Rhizomucor pusillus and Aspergillus flavus based bio-filters were equally effective for elicitation of Zn (∼30%) and Ni (>30%) toxicity. Differential minimum inhibitory concentration, uptake of HMs, bio-sorption capacity of HMs by fungi resulted in unequal efficacy of fungal bio-filters. © 2020 Elsevier B.V.