Browsing by Author "B.K. Honrao"

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Mining of Indian wheat germplasm collection for adult plant resistance to leaf rust
(Public Library of Science, 2019) Sundeep Kumar; B.S. Phogat; V.K. Vikas; A.K. Sharma; M.S. Saharan; Amit Kumar Singh; Jyoti Kumari; Rakesh Singh; Sherry Rachel Jacob; G.P. Singh; M. Sivasamy; P. Jayaprakash; M. Meeta; J.P. Jaiswal; Deep Shikha; B.K. Honrao; I.K. Kalappanavar; P.C. Mishra; S.P. Singh; S.S. Vaish; V.A. Solanki
Leaf rust (Puccinia triticina Eriks.) is a fungal disease of wheat (Triticum spp.), which causes considerable yield loss. Adult plant resistance (APR) is one of the most sustainable approaches to control leaf rust. In this study, field-testing was carried out across ten different locations, followed by molecular screening, to detect the presence of APR genes, Lr34+, Lr46+, Lr67+ and Lr68 in Indian wheat germplasm. In field screening, 190 wheat accessions were selected from 6,319 accessions based on leaf tip necrosis (LTN), disease severity and the average coefficient of infection. Molecular screening revealed that 73% of the accessions possessed known APR genes either as single or as a combination of two or three genes. The occurrence of increased LTN intensity, decreased leaf rust severity and greater expression of APR genes were more in relatively cooler locations. In 52 lines, although the presence of the APR genes was not detected, it still displayed high levels of resistance. Furthermore, 49 accessions possessing either two or three APR genes were evaluated for stability across locations for grain yield. It emerged that eight accessions had wider adaptability. Resistance based on APR genes, in the background of high yielding cultivars, is expected to provide a high level of race non-specific resistance, which is durable. © 2019 Kumar et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Multi-environment analysis to unravel bread wheat core collection to identify donors for grain quality, phenology, and yield traits
(CSIRO, 2024) Jyoti Kumari; R.K. Gupta; Arun Gupta; B.K. Honrao; S.S. Vaish; Achla Sharma; Sewa Ram; Gopalareddy Krishnappa; Shivani Sharma; Rakesh Bhardwaj; Sherry Rachel Jacob; Sundeep Kumar; V.K. Vikas; Sushil Pandey; J.C. Rana; Ashok Kumar; G.P. Singh; Kuldeep Singh
Context. Untapped wheat germplasm is conserved globally in genebanks. Evaluating it for grain quality and yield will help achieve nutritional and food security. Aims. We aimed to evaluate the Indian National Genebank bread wheat core collection for grain quality, phenology and yield, to identify potential donor germplasm. Methods. 1485 accessions were grown at three locations in India during winter 2015–2016 to evaluate test weight, grain protein content, sedimentation value (SV), days to spike emergence, days to maturity, grain yield and thousand-grain weight (TGW). Key results. Best linear unbiased estimates indicated mean protein of 13.3%, 14.7%, and 13.0% and yield of 73.0 g/m, 70.9 g/m and 66.6 g/m at Ludhiana, Pune, and Varanasi locations, respectively. The SV ranged from 26.6–65.6 mL and 17.7–66.6 mL at the Ludhiana and Pune locations, respectively. The top 10 accessions were identified for all the studied traits. Six high protein accessions, with consistent protein of more than 15% along with moderate Thousand-grain and test weights were further validated and assessed for stability across environments. Grain protein content was correlated negatively with thousand-grain weight and yield, but positively with days to maturity and spike emergence. Conclusion. The identified accessions with high trait values could be used in future breeding programmes to develop high yielding biofortified cultivars to address protein malnutrition and also cultivars with suitable end-product quality. Implications. The diversity in a core collection can be exploited to develop modern high yielding bread wheat cultivars with higher grain protein content and suitable end-product quality. © 2024 The Author(s).