Browsing by Author "Bijoy K. Roy"

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Analysis of genetic variation among accessions of wild and cultivated species of Amaranthus based on RAPD and SDS-PAGE markers
(2012) Reema Srivastava; Bijoy K. Roy
Genetic diversity and relationships among 12 cultivated and wild Amaranthus species were examined using protein and RAPD markers. High level of genetic diversity was common within species contrary to genetic uniformity within most accessions. On average, the polymorphism reached 42.60% among the cultivars but raised to 46.88% in the wild counterpart. The seed protein content varied in the 11.80-17% range and seeds of amaranth proved highly nutritive. The SDS-PAGE analysis indicated that such proteins in case of amaranths were highly heterogenous with 8-18 bands. Based on the electrophoretic mobility, such bands were assigned four zones (A, B, C and D). The RAPD analyses of Amaranthus species are further substantiated by the seed protein profile, indicating that a combination of such approaches can offer relatively reliable parameters to unravel genetic diversity.
Characterization of blue and white varieties of Clitoria ternatea (L.) based on seed protein profiling collected from North India
(International Journal of Pharma and Bio Sciences, 2015) Tara Chandra Ram; Bijoy K. Roy
The medicinal plant Clitoria ternatea belongs to the family fabaceae and their seeds are highly nutritive. In the present study characterization of seed proteins of its popularly growing varieties has been undertaken. A comparative study has been done in term of qualitative estimation of total seed protein among the six accessions of white and blue petal varieties. Three accessions (ACCRI, ACCR2 and ACCR3) of blue petal variety and three accessions (ACCR4, ACCR5 and ACCR6) of white petal variety were taken. With the help of protein molecular markers and electrophoresis pattern, seed proteins of above two varieties showed the presence of four categories of polypeptide masses. Some bands were seen in both the varieties shows varietal differences and genetic composition. A dendrogram was made using UPGMA which revealed the interrelationship of two varieties of Clitoria that indicates two clustures viz. clusture A and clusture B. Clustures A suggested that there are no changes in protein profiling among ACCR1, ACCR2 and ACCR3, but different with protein profiling among clusture B (ACCR4, ACCR5 and ACCR6).
Effect of varying pH on protein composition and yield of amaranth seed (Amaranthus blitum)
(Triveni Enterprises, 2011) Reema Srivastava; Bijoy K. Roy
The isolation procedure of the seed proteins of Amaranthus blitum have been analyzed at different pH conditions. Qualitative studies were carried out by using electrophoretic technique sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). Mainly four protein fractions i.e. albumin, globulin, prolamin and glutelin were obtained. Protein isolates were prepared by (a) extraction at different alkaline pH (9, 10, 11 and 12) and precipitation at pH 5 and (b) extraction at pH 9 and precipitation at different pH (4, 5, 6 and 7). The composition of isolates, prepared by method (a), depended on extraction pH. The isolate extracted at pH 8 was mainly composed of albumin and globulin, whereas at pH 9, 10 and 11 showed the presence of prolamin and glutelin. Electrophoretic pattern of different isolates had five major bands with molecular weight of 30, 45, 72, 84 and 90 kDt respectively. The increase of the extraction pH led to the increase in protein yield. With method (b) isolates obtained were variable in composition. At pH 7 albumin and prolamine were present, whereas at pH 4, 5 and 6 all 4 protein fractions were precipitated. According to the quantitative estimation of the albumin, globulin, prolamin and glutelin in the seed flour the contents were 26.4, 25, 5.81 and 42.7%, respectively. The results suggest that composition of protein isolates could be controlled by different extraction and precipitation pH. © 2011 Triveni Enterprises.
In Silico Profiling of the Potentiality of Curcumin and Conventional Drugs for CagA Oncoprotein Inactivation
(Wiley-VCH Verlag, 2015) Akhileshwar K. Srivastava; Mallika Tewari; Hari S. Shukla; Bijoy K. Roy
The oncoprotein cytotoxic associated gene A (CagA) of Helicobacter pylori plays a pivotal role in the development of gastric cancer, so it has been an important target for anti-H. pylori drugs. Conventional drugs are currently being implemented against H. pylori. The inhibitory role of plant metabolites like curcumin against H. pylori is still a major scientific challenge. Curcumin may represent a novel promising drug against H. pylori infection without producing side effects. In the present study, a comparative analysis between curcumin and conventional drugs (clarithromycin, amoxicillin, pantoprazole, and metronidazole) was carried out using databases to investigate the potential of curcumin against H. pylori targeting the CagA oncoprotein. Curcumin was filtered using Lipinski's rule of five and the druglikeness property for evaluation of pharmacological properties. Subsequently, molecular docking was employed to determine the binding affinities of curcumin and conventional drugs to the CagA oncoprotein. According to the results obtained from FireDock, the binding energy of curcumin was higher than those of amoxicillin, pantoprazole, and metronidazole, except for clarithromycin, which had the highest binding energy. Accordingly, curcumin may become a promising lead compound against CagA+ H. pylori infection. The oncoprotein cytotoxic associated gene A (CagA) is an important target for anti-Helicobacter pylori drugs. By molecular docking to CagA, a comparative analysis was carried out between curcumin and conventional drugs such as clarithromycin, amoxicillin, pantoprazole, and metronidazole. The results show that curcumin may become a promising lead compound against CagA+ H. pylori infection. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Novel source of resistance and differential reactions on chilli fruit infected by Colletotrichum capsici
(2013) Ruchi Garg; Sanjay Kumar; Rajesh Kumar; Murugan Loganathan; Sujoy Saha; Sanjeet Kumar; Awadhesh B. Rai; Bijoy K. Roy
Pre- and post-harvest anthracnose fruit rot is a main disease of chilli (Capsicum spp.). Among Colletotrichum species causing anthracnose, C. capsici is the most common in India. A total of 41 Capsicum genotypes were screened for anthracnose resistance under field conditions. Bhut Jolokia, PBC-380 and IC-383072 were found symptomless and some marginally cultivated lines and other crosses were observed to be resistant. In vitro inoculation of the selected set of genotypes with C. capsici revealed that 9 days after inoculation was appropriate to record observations on disease resistance. Screening the 41 genotypes for resistance to anthracnose under in vitro conditions revealed four symptomless and 11 highly resistant lines. Comparing field and in vitro evaluations, nine lines (BS-35, BS-20, BS-28, Punjab Lal, Bhut Jolokia, Taiwan-2, IC-383072, Pant C-1 and Lankamura Collection) showed consistent resistance in field as well as in vitro to the disease. The study on differential reactions on fruits of 16 genotypes inoculated by the two C. capsici isolates (Ccf-Varanasi and Ccc2-Raichur) clearly showed that these isolates are two different pathotypes. A detailed analysis on prevalent strains in the target region would be needed to initiate resistance breeding. Nevertheless, newly identified resistant sources offer better choices for the ongoing anthracnose resistance breeding program. © 2012 Australasian Plant Pathology Society Inc.
Proteomic analysis of different extracts from Amaranth (Amaranthus tricolor) grains
(2013) Reema Srivastava; Bijoy K. Roy
Amaranth seed has a high nutritional value due to the balanced composition of its protein fractions (i.e albumins, globulins, prolamins and glutelins). These were isolated by sequential extractions. The amount of albumins, globulins, prolamins and glutelins were 2.90, 2.20, 0.47 and 5.73 g/100g of seed flour. Upon electrophoresis, albumins were found to be most polymorphic, while prolamins were made up of fewer and less abundant components. Gel electrophoresis analysis revealed the occurrence of glutelins as the major proteins in the seed. Amaranth protein isolates were prepared by (a) extraction at different alkaline pHs and precipitation at pH 5 and (b) extraction at pH 9 and precipitation at different pHs. The isolates were compared with protein fractions. Acidification at pH 5 and precipitation at lower pHs lead to denaturation of the proteins which was partially reversed by returning to pH 7.