Browsing by Author "Bishal Kumar Singh"

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Biophysical characterization of fibroblast growth factor homologous factor-1b (FHF-1b): Sodium dodecyl sulfate promotes two state folding
(Bentham Science Publishers B.V., 2008) Vikash Kumar Dubey; Bishal Kumar Singh; Nandini Sarkar; Monu Pande; Medicherla Venkata Jagannadham
The current article describes the biophysical characterization and folding studies of fibroblast growth factor homologous factor-1b (FHF-1b) in comparison with acidic fibroblast growth factor (FGF-1). Our data indicates that FHF-1 is significantly more stable than FGF-1. The folding mechanism of these two proteins seems to be different although they share high degree of sequence and structural similarity. FHF-1 unfolds through stable intermediate state while unfolding of FGF-1 is two-state. Interestingly, low concentration of sodium dodecyl sulfate (SDS) drives the folding pathway of FHF-1b to two-state. © 2008 Bentham Science Publishers Ltd.
Papain-like proteases: Applications of their inhibitors
(2007) Vikash K. Dubey; Monu Pande; Bishal Kumar Singh; Medicherla V. Jagannadham
Proteases are one of the most important classes of enzyme and expressed throughout the animal and plant kingdoms as well as in viruses and bacteria. The protease family has drawn special attention for drug target for cure of several diseases such as osteoporosis, arthritis and cancer. Many proteases from various sources are being studied extensively with respect to activity, inhibition and structure. In this review, we hope to bring together the information available about the proteases with particular emphasis on papain-like plant cysteine proteases. Besides, protease inhibitors and their potential utilities are also discussed. © 2007 Academic Journals.