Browsing by Author "Brahma Swaroop Dwivedi"

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Effect of fluorescent Pseudomonas on plant growth promotion of Aloe vera
(Taylor and Francis Ltd., 2024) Anuradha Rai; Vaibhav Kumar Singh; Naveen Kumar Sharma; Jay Shankar Singh; Vinod Kumar Singh; Brahma Swaroop Dwivedi; Pradeep Kumar Rai
Modern agriculture practices heavily depend on the application of chemical fertilizers and continuous application disturbs the soil quality and health. Fluorescent Pseudomonas are the well characterized group of bacteria proficient in plant growth promotion and have excellent phosphate solubilizing abilities and solubilize insoluble phosphate to plants available form via secretion of organic acids. This study focused on the phosphate solubilization ability of Pseudomonas, arouse of Aloin-A molecule, gluconic acid production, as well as biometric parameters of key medicinal plant, the Aloe vera (Aloe barbadensis). Two strains (P. putida and Pseudomonas sp.) were found very effective PSBs and solubilized 236.73 and 153.73 mg L−1 phosphate, respectively. High-performance liquid chromatography analysis (HPLC) showed the production of gluconic acid (GA). P. putida and P. sp. produced 23970 ± 231 and 21538 ± 129 mg L−1 GA, respectively. Inoculation of the plants with consortium of P. putida and Pseudomonas sp. enhanced the plant growth, soil available P and phosphate uptake in plants than individual inoculation. An increase in aloin-A content (18.15 and 28.71 fold) was recorded in A. barbadensis treated with a PSB consortium in a soil amended without and with TCP (tricalcium phosphate), respectively. P-uptake ultimately enhances the aloin-A synthesis and biomass of plant. Therefore, agronomic attributes of Aloe vera plants can be improved for used as a medicinal plant through application of potent Fluorescent Pseudomonas. © 2023 Taylor & Francis Group, LLC.
Study of Phosphate Solubilizing Fluorescent Pseudomonas Recovered from Rhizosphere and Endorhizosphere of Aloe barbadensis (L.)
(Taylor and Francis Ltd., 2023) Anuradha Rai; Naveen Kumar Sharma; Vinod Kumar Singh; Brahma Swaroop Dwivedi; Jay Shankar Singh; Pradeep Kumar Rai
Phosphorus (P) is a key nutrient required by plants for their growth and development. A large part of applied phosphatic fertilizer becomes unavailable due to its fixation in soil. In rhizosphere, fluorescent Pseudomonads are instrumental in phosphate solubilization. We isolated Pseudomonads from the rhizosphere of Aloe barbadensis (L.), and screened them for their plant growth promoting ability such as - solubilization of phosphate, auxin production and 1-aminocyclopropane-1-carboxylate (ACC) deaminase activity. A total of 307 fluorescent Pseudomonas were isolated and screened, of which, 24 isolates showed a strong phosphate solubilizing ability, ranging from 69.71 to 236.73 µg ml−1. The Indole acetic acid (IAA) production by all 24 isolates varied from 28.44 to 151.99 µg ml−1. However, 17 isolates produced ACC-deaminase ranging from 8.19 to 10.27 µmol mg−1 h−1. Analyses of 16S rRNA gene and Pseudomonas-specific genes grouped the isolates in seven different species of fluorescent Pseudomonas. Genotypic analysis based on 16S rDNA-RFLP using restriction enzymes (HaeIII, AluI and MspI) and BOX-PCR generated unique genotype specific pattern. The results suggested that Pseudomonas diversity has no association with microenvironments and the sampling site of the plants. 16S rRNA gene-based diversity indices revealed great diversity among the fluorescent Pseudomonas recovered from the rhizospheric regions of the Aloe. Due to the above plant growth-promoting traits, fluorescent Pseudomonas can be exploited as bio-inoculants for crops, with minimal damage to the environment. © 2023 Informa UK Limited, trading as Taylor & Francis Group.