Browsing by Author "Brajesh Sinha"

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Comparative Study of Crossability Behavior in Intra-specific and Inter-specific Crosses of Vigna radiata and Vigna mungo
(Agricultural Research Communication Centre, 2023) Varsha Singh; Anil Kumar Singh; Mahendra Narain Singh; Brajesh Sinha
Background: The basic plant material consisted of 9 mungbean and 5 urdbean genotypes. 42 different crosses were made comprising 13 Vigna radiata ´ V. radiata, 4 V. radiata ´ V. mungo and 25 V. radiata ´ V. mungo crosses. Varying degree of success has been achieved in obtaining intraspecific and interspecific crosses having desired variability for yield and yield contributing traits along with MYMV resistance. Methods: The present investigation was done at Agricultural Research Farm, Banaras Hindu University, Varanasi, during Kharif, 2019 and Summer, 2020. 42 intra-specific and inter-specific cross combinations were initially examined for their crossability. Out of these, six promising crosses with higher crossability percent were further analyzed for hybrid pollen fertility, hybrid lethality and, evaluation of F1 and parents for different yield traits. Result: Crossability per cent found highest in intraspecific cross HUM 2 ´ IPM 02-3 (41.86%) in mungbean while, in urdbean cross NDU 1 ´ R3/12 (41.38%). In interspecific crosses, the maximum crossability per cent was found in SKAU M 365 ´ R3/28 (34.92%). Two mungbean genotypes (HUM 2 and HUM 26) and two urdbean genotypes (R3/12 and R3/28) can be further utilized for genetic improvement through inter-specific hybridization as there cross combinations are better performing on the basis of yield attributes along with MYMV resistance. © 2023, Agricultural Research Communication Centre. All rights reserved.
Identification of transcription factors in tomato, potentially related to early blight resistance at invasion in host tissue using, microarray expression profiling
(Elsevier B.V., 2016) Priti Upadhyay; Showkat Hussain Ganie; Ashutosh Rai; Major Singh; Brajesh Sinha
Tomato early blight is an important threat due to its capacity to reduce the production in all major tomato producing areas. Molecular mechanisms underlying resistance to the causal organism are not well known. Therefore, we aimed to study tomato – Alternaria solani system to search the transcription factors and pathways which, are responsible for resistance to this fungi using, affymetrix gene chip for tomato. three hundred ninety five transcription factors were found to be differentially expressed at 24 h after inoculation with A. solani in the resistant genotype, EC-520,061, of tomato. Also, Zinc Finger Proteins, Ribosomal binding unit S4 and Auxin responsive transcription factors were found to play significant role in resistance. Their expression has enhanced the pathogenesis related proteins and also other proteins as well, which, have direct role in stopping the penetration of mycelia in host plant. © 2016 South African Association of Botanists
Microarray analyses during early stage of the tomato/Alternaria solani interaction
(Elsevier Inc., 2015) Priti Upadhyay; Ashutosh Rai; Rajesh Kumar; Major Singh; Brajesh Sinha
Tomato early blight is an important threat and it has capacity to reduce the production in all major tomato producing areas. Molecular mechanism underlying the resistance against this is not well known. Therefore we studied this system to search the possible mechanism of resistance, which includes pathogenesis related protein, and pathways and transcription factors, which are responsible for resistance against this pathogen using affymetrix gene chip for tomato. Their differential expressions have enhanced the biochemical and other related products, which have, direct or indirect role in stopping the penetration of mycelia in the host plant. © 2015 The Authors. Published by Elsevier Inc.