Browsing by Author "Brij Mohan Singh"

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Characterization of Swarna Bhasma and Swarnaprash and Determination of Antimicrobial Properties Against Gut-Pathobiont and Symbiont
(Springer, 2025) Kavita Singh; Prashant Kumar Gupta; Awanish Kiran Kumar; Brij Mohan Singh
Ayurveda has a long-standing tradition of healthcare in Southeast Asia. Swarnaprash, a classical Ayurveda preparation, is commonly given as the pre-lacteal feed to neonates to prevent labor-related complications and infections. It comprises incinerated gold particles (InAuP/Swarna Bhasma), honey (Madhu), and clarified butter oil (CBO/Cow Ghrita). This in vitro study aimed to evaluate the therapeutic potential of the individual ingredients and combinations of Swarnaprash against selected neonatal gut pathobionts and symbionts. The study employed sophisticated instruments, including SEM with EDAX and X-ray diffraction analysis, to investigate the shape and structural disparities in the ingredients of Swarnaprash. The reported size of gold particles in Swarnaprash ranges from 0.6 to 9.5 µm. These particles are relatively smaller than those in Swarna Bhasma but larger than synthetic gold particles. Swarnaprash demonstrated both bactericidal and bacteriostatic activity against selected neonatal gut pathobionts, with the largest inhibition zones observed for P. aeruginosa and S. Typhi. It surpassed the individual efficacy of its components—Prash, InAuPs, honey, or CBO alone. Notably, Swarnaprash did not affect the selected beneficial gut bacteria. The results warrant further in vivo and clinical studies to explore the effects of Swarnaprash on neonatal gut flora, which would provide vital information for research in neonatal healthcare. © The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2024.
Characterization of Swarna Bhasma and Swarnaprash and Determination of Antimicrobial Properties Against Gut-Pathobiont and Symbiont
(Springer, 2024) Kavita Singh; Prashant Kumar Gupta; Awanish Kumar; Brij Mohan Singh
Ayurveda has a long-standing tradition of healthcare in Southeast Asia. Swarnaprash, a classical Ayurveda preparation, is commonly given as the pre-lacteal feed to neonates to prevent labor-related complications and infections. It comprises incinerated gold particles (InAuP/Swarna Bhasma), honey (Madhu), and clarified butter oil (CBO/Cow Ghrita). This in vitro study aimed to evaluate the therapeutic potential of the individual ingredients and combinations of Swarnaprash against selected neonatal gut pathobionts and symbionts. The study employed sophisticated instruments, including SEM with EDAX and X-ray diffraction analysis, to investigate the shape and structural disparities in the ingredients of Swarnaprash. The reported size of gold particles in Swarnaprash ranges from 0.6 to 9.5 µm. These particles are relatively smaller than those in Swarna Bhasma but larger than synthetic gold particles. Swarnaprash demonstrated both bactericidal and bacteriostatic activity against selected neonatal gut pathobionts, with the largest inhibition zones observed for P. aeruginosa and S. Typhi. It surpassed the individual efficacy of its components—Prash, InAuPs, honey, or CBO alone. Notably, Swarnaprash did not affect the selected beneficial gut bacteria. The results warrant further in vivo and clinical studies to explore the effects of Swarnaprash on neonatal gut flora, which would provide vital information for research in neonatal healthcare. © The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2024.
Clinical and metabolic markers based study of Swas Kasa Chintamani Rasa (An Ayurvedic herbo-metallic preparation) in childhood bronchial asthma (Tamak Swas)
(BRNSS Publication Hub, 2014) Yogesh Kumar; Brij Mohan Singh; Prashant Gupta
Background: Asthma is a problem worldwide, with an estimated 300 million affected individuals. It is a disease of the respiratory system, characterised by intermittent inflammation, constriction or obstruction of the airways leading to a temporary reduction in airflow, and associated shortness of breath. Aims: In Ayurveda, there are lots of drugs to treat the bronchial asthma (Tamak Swas), among them Swas-Kasa-Chintamani Rasa (SKCR) is considered a good drug to cure. Materials and Methods: A total of consented 23 children of both sex under 12 years of age were included in the study and divided into three groups, blood samples were collected before treatment and after the completion of therapy for the metabolic markers like Hb gm%, TLC, AEC, S. Protein, S. Albumin, SGOT, SGPT, alkaline phosphatase and S. Bilirubin. SKCR was given for a total of 45 days in a dose of 4 mg/kg/dose × 12 hourly with garlic, ginger and honey in ratio of 1:2:4. Statistical Analysis Used: In the present study, SPSS software was used to get statistical data such as Mean (X-), Mean Difference (d′), Standard Deviation (SD) and Student′s "t" test, etc. Results and Conclusions: The findings suggest that the drug is more effective in those cases who are not receiving corticosteroid with bronchodilator in comparison to children receiving corticosteroids with/without bronchodilator. No specific adverse effect of drug SKCR was observed.
Efficacy of Swarna-prash in combating perinatal oxidative stress and its compatibility in newborns
(National Institute of Science Communication and Policy Research, 2024) Smriti Singh; Kavita Singh; Brij Mohan Singh; Yamini Bhushan Tripathi
All neonates are exposed to a variable degree of oxidative stress that may result in cellular, tissue, or organ damage due to a low-efficient antioxidant system. The present study aimed to evaluate the effect of licked Swarna-prash, comprising 15 mg Swarna-bhasma (incinerated gold particles) mixed with 1 mL honey and 0.5 mL Ghrita (butter oil), given in perinatal oxidative stress. A randomized case-control study ensued after the microanalysis of InAuPs (Incinerated Aurum Particles) and Swarna-prash. Ninety newborns were registered, considering the inclusion and exclusion criteria, and divided into three groups. The single dose of Swarna-prash was given once a day in Group A and thrice a day in Group B. Due to ethical issues, nothing was given except mother milk in Group-C (control group). Venous blood samples were collected from the umbilical cord after birth and 48 h for complete blood counts (CBC), Liver function test (LFT), Renal function tests (RFT), and antioxidant enzymes. The Swarna-bhasma consisted of crystallite-size InAuPs ranging from 30.86 to 114.02 nm. The intergroup analysis of CBC, LFT, RFT, SOD, GSH, and catalase values shows in significant (p>0.05) variation except for the serum bilirubin and SOD in group-B v/s C, offers significant mean values (p<0.05). Finally, it can be inferred that Swarna-prash opposes neonatal perinatal oxidative stress, seems nontoxic, reduces the incidence of physiological jaundice, and is bio-compatible. © 2024, National Institute of Science Communication and Policy Research. All rights reserved.
In vitro analysis of synergistic effect of honey against chemically induced hepatic insult in Balb/c mice
(Research Journal of Pharmacy and Technology, 2021) Prashant Kumar Gupta; Chandradeo Narayan; Kamal Shah; Arvind Kumar; Brij Mohan Singh
Human being knows honey since thousands of years for its nutritional and medicinal values. Traditional medicine systems like Ayurveda have elaborated honey as boon for health and patient care. Synergistic effect of honey was research but to a limited extend. Honey is advised with Picrorhiza kurroa for hepatic disorders or hepato-protection in Ayurveda. Here we have examined in vitro synergistic effect of honey when given with Picrorhiza kurroa in acetaminophen-induced hepatotoxicity in Balb/c mice model. We obtained Serum and liver lysate to check levels of hepatic markers, Alkaline phosphatase and Glutathione. The level of alkaline phosphatase raised while glutathione level is reduced during hepatic offence. We observed a compensation of above markers when P. Kurrua, honey and its combination were used in acetaminophen induced hepatic toxicity. We observed a better mice weight gain in combination group (Group IV) compared to control group. This study can pave a way for future research on honey as a better adjuvant for hepatotoxic drugs and other herb-drug interactions researches. © RJPT All right reserved.
Microanalysis oriented bactericidal effect of the Suvarna Bhasma containing incinerated gold nanoparticles against the Pseudomonas Aeruginosa
(Association of Ayurvedic Physicians of India, 2025) Sahana Shankari; Brij Mohan Singh; Gopal Nath; Anchal Srivastava
The antibacterial activity of synthetically prepared gold nanoparticles is well-known. The present unique study was done to test the Suvarna bhasma containing the incinerated gold particles for its bactericidal activity against Pseudomonas aeruginosa. The Microanalysis of Suvarna bhasma was characterized by scanning electron microscopy, which revealed the gold particles’ size ranges from 28 nm to 8.1 µm, forming particle agglomerates of a spherical shape. It has a rough surface with a heterogeneous distribution. The transition electron microscopy revealed that the particle size ranged between 2.2 to 18.96nm after the sonication of Suvarna bhasma. The energy dispersed X-ray analysis of the Suvarna bhasma sample revealed that it contained 86.3% gold, 3.1% oxygen, and 10.5% carbon. The X-ray diffraction findings of Suvarna bhasma showed four intense peaks of pure gold nanoparticles at (111), (200), (220), and (311) depicting that gold existed in a face-centered cubic crystalline lattice phase with an average particle size of 27.58 nm. The Minimum Inhibitory Concentration of Suvarna bhasma, through the broth microdilution method, was seen to be 312µg/mL against Pseudomonas aeruginosa ATCC 27853 grown in brain-heart infusion. In contrast, the Minimum Bactericidal Concentration was 1250 µg/mL. This result was substantiated by scanning electron microscopy analysis of the P. aeruginosa (106 colony forming units) treated with 1000 µg/mL of Suvarna bhasma, showing cellular structural damage as membrane rupture, cell shrinking, and death. Hence, Suvarna bhasma may be one of the futuristic antipseudomonal agents. © 2025, Association of Ayurvedic Physicians of India. All rights reserved.