Browsing by Author "Chandra B. Patel"

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Q-PCR based culture-independent enumeration and detection of enterobacter: An emerging environmental human pathogen in riverine systems and potable water
(Frontiers Research Foundation, 2016) Chandra B. Patel; Rishi Shanker; Vijai K. Gupta; Ram S. Upadhyay
The availability of safe and pristine water is a global challenge when large numbers of natural and anthropogenic water resources are being depleted with faster rate. The remaining water resources are severely contaminated with various kinds of contaminants including microorganisms. Enterobacter is one of the fecal coliform bacteria of family Enterobacteriaceae. Enterobacter was earlier used as an indicator bacterium along with other fecal Coliforms namely Escherichia coli, Citrobacter, and Klebsiella, but it is now known to cause various diseases in human beings. In this study, we have collected 55 samples from potable water and riverine system and proved their presence using their conserved sequences of 16S rRNA and 23S rRNA genes with the help of SYBR green real-time PCR, which showed very high specificity for the detection of Enterobacter. The Enterobacter counts in potable water were found to 1290 ± 32.89 to 1460 ± 39.42 cfu/100 ml. The Enterobacter levels in surface water were 1.76 × 104 ± 492, 1.33 × 104 ± 334, 1.15 × 104 ± 308, 2.56 × 104 ± 802, 2.89 × 104 ± 962, 8.16 × 104 ± 3443 cfu/100 ml; the levels of Enterobacter contamination associated with hydrophytes were 4.80 × 104 ± 1804, 3.48 × 104 ± 856, 8.50 × 104 ± 2074, 8.09 × 104 ± 1724, 6.30 × 104 ± 1738, 3.68 × 104 ± 949 cfu/10 g and the Enterobacter counts in sediments of the river, were 2.36 × 104 ± 703, 1.98 × 104 ± 530, 9.92 × 104 ± 3839, 6.80 × 104 ± 2230, 8.76 × 104 ± 3066 and 2.34 × 104 ± 732 cfu/10 g at the sampling Site #1, Site #2, Site #3, Site #4, Site #5, and Site #6, respectively. The assay could be used for the regular monitoring of potable water and other water reservoirs to check waterborne outbreaks. © 2016 Patel, Shanker, Gupta and Upadhyay.
Role of Penicillium Species in Bioremediation Processes
(Elsevier, 2017) Andleeb Zehra; Manish K. Dubey; Mukesh Meena; Mohd Aamir; Chandra B. Patel; Ram S. Upadhyay
Environmental pollution, particularly that caused by various industrial activities, is responsible for the accelerated flux of organic and inorganic matter in the ecosphere. Xenobiotics such as phenolic compounds, polycyclic aromatic hydrocarbons, and heavy metals, even at low concentrations, can be toxic to humans and other lifeforms. Various types of physical, chemical, and biological treatment techniques are in use for the remediation process of contaminated soil and water. Bioremediation is a pollution-control technology that uses biological systems to catalyze the degradation or transformation of various toxic chemicals to less harmful forms. The biological process of remediation is superior over others because it leaves fewer harmful byproducts. © 2018 Elsevier B.V. All rights reserved.