Browsing by Author "Charu Lata"

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An overview on miRNA-encoded peptides in plant biology research
(Academic Press Inc., 2021) Ankita Yadav; Indraneel Sanyal; Shashi Pandey Rai; Charu Lata
MicroRNAs (miRNAs) are short (21–23 nt) regulatory RNA molecules present in plants and animals which are known for regulating the mRNA target gene expression either by cleavage or translational repression. With the advancements in miRNAs research in plants towards their biogenesis and applications has directed the recent discovery of pri-miRNAs encoding functional peptides or microRNA peptides (miPEPs). These miPEPs are encoded by 5′ of pri-miRs containing short ORFs (miORFs). miPEPs are known to enhance the activity of their associated miRNAs by increasing their accumulation and hence downregulating the target genes. Since miPEPs are very specific for each miRNA, they are considered as novel and effective tools for improving traits of interest for plant growth promotion and plant-microbe interaction. Entire peptidome research is the need of the hour. This review thus summarizes recent advancements in miPEP research and its applications as a technology with important agronomical implications with miRNAs augmentation. © 2021 Elsevier Inc.
microRNA 166: an evolutionarily conserved stress biomarker in land plants targeting HD-ZIP family
(Springer, 2021) Ankita Yadav; Sanoj Kumar; Rita Verma; Charu Lata; Indraneel Sanyal; Shashi Pandey Rai
MicroRNAs (miRNAs) are significant class of noncoding RNAs having analytical investigating and modulatory roles in various signaling mechanisms in plants related to growth, development and environmental stress. Conserved miRNAs are an affirmation of land plants evolution and adaptation. They are a proof of indispensable roles of endogenous gene modulators that mediate plant survival on land. Out of such conserved miRNA families, is one core miRNA known as miR166 that is highly conserved among land plants. This particular miRNA is known to primarily target HD ZIP-III transcription factors. miR166 has roles in various developmental processes, as well as regulatory roles against biotic and abiotic stresses in major crop plants. Major developmental roles indirectly modulated by miR166 include shoot apical meristem and vascular differentiation, leaf and root development. In terms of abiotic stress, it has decisive regulatory roles under drought, salinity, and temperature along with biotic stress management. miR166 and its target genes are also known for their beneficial synergy with microorganisms in leguminous crops in relation to lateral roots and nodule development. Hence it is important to study the roles of miR166 in different crop plants to understand its defensive roles against environmental stresses and improve plant productivity by reprogramming several gene functions at molecular levels. This review is hence a summary of different regulatory roles of miR166 with its target HD-ZIP III and its modulatory and fine tuning against different environmental stresses in various plants. © 2021, Prof. H.S. Srivastava Foundation for Science and Society.
Overexpression of miR166 in Response to Root Rhizobacteria Enhances Drought Adaptive Efficacy by Targeting HD-ZIP III Family Genes in Chickpea
(Springer Science and Business Media Deutschland GmbH, 2024) Ankita Yadav; Sanoj Kumar; Rita Verma; Shiv Narayan; Uma Gupta; Charu Lata; Shashi Pandey Rai; Indraneel Sanyal
Using the transgenic approach, the current study investigated the tripartite interaction of miRNA166, Plant Growth Promoting Rhizobacteria (PGPR), and chickpea crops in response to drought. miR166, an evolutionarily conserved small RNA, was cloned and transformed in a homologous manner. This Car-miR166 is reported in our previous research to have drought-enduring roles in response to microbial candidates. A Pseudomonas putida strain RA (MTCC5279) is used as a PGPR for the whole study. The overexpressed lines generated using tissue-culture practice were functionally validated with physiological parameters studied using Li-Cor 6400XT, including photosynthesis rate, transpiration rate, water-use efficiency, and electron transport rate. We also studied the relative water content of the overexpressed lines in comparison to treated control plants. In biochemical methods, we studied the accumulation of proline, superoxide dismutase, peroxidase, catalase, H2O2 and lipid peroxidation levels. miR166 has its target as ATHB15 (Homeobox-leucine zipper protein-15) validated using 5’ RNA Ligase-Mediated Rapid Amplification of cDNA Ends (RLM-RACE) experiment. At the molecular levels, we carried out the stem-loop quantitative real-time (qRT) PCR analysis of miR166 and the expression analysis of ATHB15 in transgenic lines. As per our study, the results reported that the transgenic lines showed a positive interaction of miR166 with PGPR, resulting in drought stress mitigation and better plant survival in harsh drought conditions. In conclusion, the physiology, biochemistry, and molecular expression levels of Car-miR166 (Cicer arietinum L.) in transgenic lines in response to PGPR support enhanced growth and development in response to PGPR in transgenic lines under drought. © The Author(s) under exclusive licence to Sociedad Chilena de la Ciencia del Suelo 2024.
Overexpression of PGPR responsive chickpea miRNA166 targeting ATHB15 for drought stress mitigation
(Springer Science and Business Media B.V., 2023) Ankita Yadav; Sanoj Kumar; Rita Verma; Shiv Narayan; Ram Jatan; Charu Lata; Shashi Pandey Rai; Pramod A. Shirke; Indraneel Sanyal
Water limitation creates drought-like situations and constrains the life cycle of crop plants by modulating their biological processes at physiological, biochemical, and molecular levels. The microbial measures, including plant growth-promoting rhizobacteria (PGPR), could be used in plant adaptation. These PGPR escape water scarcity conditions and relates to plants by modulating several microRNAs in plant stress responses. The present study relates the beneficial role of PGPR (Pseudomonas putida-RA) responsive Car-miR166 of chickpea in drought mitigation with phytohormonal crosstalk in transgenic Arabidopsis lines. The transgenic lines showed an increased percentage of seed germination in comparison to treated control plants with highest germination rate in T2 (90%) and highest root length was observed in drought treated inoculated T1 lines (29%) under 300 mM of mannitol. The various physiological parameters including photosynthesis rate, transpiration rate, water-use efficiency and stomatal conductance were also better along with lower electrolyte leakage and higher relative water content in treated transgenic lines under inoculated conditions. The biochemical parameters including enzymatic and non-enzymatic antioxidants were improved in transgenic lines with less membrane damage and the highest accumulation of proline in T2 lines under RA inoculation and drought stress in comparison to treated control. The miR166 in drought-treated inoculated plants was highly upregulated (≥ 4) log2 fold change in T3 whereas the target was highly downregulated (≥ -2) log2 fold change in T2. Overall, our results concluded that RA-responsive Car-miR166 plays beneficial stress-mitigating roles under drought in transgenic plants, suggesting its crucial role in crop enhancement in response to PGPR. © 2023, The Author(s), under exclusive licence to Springer Nature B.V.
Target cleavage mapping and tissue-specific expression analysis of PGPR responsive miR166 under abiotic stress in chickpea (Cicer arietinum L.)
(Springer Science and Business Media B.V., 2023) Ankita Yadav; Sanoj Kumar; Rita Verma; Shashi Pandey Rai; Charu Lata; Indraneel Sanyal
Legumes are an indispensable food after cereals with extensive production across the world. Legume production is imposed with limitations and has been augmented by various environmental stresses. The symbiotic relations between legumes and rhizobacteria have been an intriguing topic of research in view of their roles in plant growth, development and various stress responses. Recent advances in gene networks involving a plethora of evolutionarily conserved miRNAs have been investigated pertaining to their roles in plant stress responses. The interaction between plant growth-promoting rhizobacteria (PGPR) strain Pseudomonas putida (RA), MTCC5279 and abiotic stress-responsive miRNAs have previously been studied with roles in abiotic stress mitigation by modulating stress-responsive miRNAs and their target genes. The present study is an investigation involving the role of RA-responsive miR166 for drought mitigation in desi chickpea genotype. Drought-stressed chickpea plants when inoculated with RA, the inverse correlation in expression patterns were noticed in miR166 and its validated target, ATHB15. miR166-directed cleavage of ATHB15 has been carried out in drought-treated plantlets upon RA inoculation using 5´RLM-RACE analysis. Tissue-specific expression patterns in 15 days old chickpea seedlings including leaves, shoot and roots when exposed to salinity, drought and abscisic acid at different time points indicating the role of miR166 in different abiotic stress responses. In view of the results, validation and functional characterization of such interactions involving stress-responsive miRNAs along with microbial applications in stress management could be an important method for crop improvement. © 2023, The Author(s), under exclusive licence to Springer Nature B.V.