Browsing by Author "D. Gupta"

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Bridging large bone defects with a xenograft composited with autologous bone marrow - An experimental study
(Springer-Verlag, 1982) D. Gupta; S. Khanna; S.M. Tuli
We have investigated the role of defatted, decalcified xenogenic bone implants composited by impregnation with fresh autologous marrow in bridging large cortical defects. Diaphyseal cortical implants were obtained from orthopaedic operations on humans and from guinea pigs and prepared by treatment with acetone, 0.6 M HCl and ethanol. The composited graft was used to bridge a 3 cm defect in the diaphysis of the ulna of rabbits. Periodic investigations were made clinically, radiographically and histologically and by tetracycline, fluorescence studies up to 20 weeks. Abundant new bone formation was observed in 85.7% of composited human xeno-autografts and in 83.3% of composited guinea pig xeno-autografts. New bone formation was seen in 13.3% of noncomposited xeno-implants. No appreciable antigenicity was observed either clinically or histologically in the grafts. The high degree of success obtained justifies further studies on implants composited by fresh autologous marrow and offers hope for the enhancement of new bone formation clinically, particularly in reconstructive operations for significant skeletal defects. We feel that the osteo-inductive potential of autologous marrow was responsible for the healing of the defects aided by the better internal milieu provided by defatted decalcified xeno implant. © 1982 Springer-Verlag.
Delayed intracerebral hematoma [1]
(1992) S.K. Gupta; D. Gupta; S.K. Gupta; V. Shankar
[No abstract available]
Estimation and correlation of serum folic acid levels in spina bifida babies and their mothers
(2008) D. Gupta; A. Pandey; A. Gangopadhyay; S. Prasad; T. Singh; H. Khanna; V. Upadhyaya
Spina bifida is a common neural tube defect. The relative deficiency of folic acid (FA) in mother during periconceptional period is an important factor. We measured the serum FA levels in spina bifida babies and their mothers and compared the result with the FA level in normal babies. The blood samples were collected at the time of admission to hospital. Enquiry made about the age, sex, and birth order, problem in siblings and FA supplementation and detailed examination done to access the severity of the problem. Folic acid estimation was done by enzyme-linked immunosorbent assay method. Antenatal FA supplementation was found in 40% of cases and 80% of control group. In the study group, the mean FA level in babies and mothers of FA supplemented group was 21.73 ± 5.64 ng/dl and 15.02 ± 3.02 ng/dl and in non-supplemented group were 7.41 ± 1.48 ng/dl and 4.12 ± 0.81 ng/dl, respectively. In control group mean FA level in babies and mothers of FA supplemented group were 16.88 ± 3.2 ng/dl and 14.70 ± 2.30 ng/dl and in nonsupplemented group were 14.33 ± 1.74 ng/dl and 10.75 ± 1.34 ng/dl, respectively. The serum FA levels in spina bifida babies as well as in their mothers were significantly low as compared to control group. This study suggests that the low serum FA levels in a lady at the time of pregnancy may be a causative factor in the pathogenesis of spina bifida and low levels in affected babies.
Investigation of treatment potential of horizontal subsurface flow constructed wetland for the treatment of secondary treated sewage
(Institute for Ionics, 2024) R. Shukla; D. Gupta; V.K. Mishra
Present study investigated the potential of different setups of horizontal subsurface flow constructed wetland (HSSFCW) for sewage treatment in the Indian context. Total four different setups, i.e., CW1 (unplanted CW); CW2 (planted with C. Benghalensis); CW3 (planted with T. Latifolia); and CW4 (planted with C. Benghalensis and T. Latifolia; 50% each of both the plants) were used to perform the treatment experiments. To perform the treatment experiment, a HSSFCW with the surface area of 35 m2 (3.5 × 10 m) and gravel as filter medium was built. Each setup of wetland remained operational for three months during which its performance was evaluated. The CW was fed at an average flow rate of 250 L h−1 with secondary treated sewage, and the treated sewage was examined twice a week at five different hydraulic retention times (HRT), i.e., 0, 12, 24, 36 and 48 h for essential sewage quality parameters. The wetlands efficiency improved proportionally with the increase in the duration of the hydraulic retention time. However, the aquatic macrophytes enhanced the HSSFCW efficiency. The CW4, i.e., the wetland planted with combination of C. Benghalensis and T. Latifolia showed the best performance in treating the biological oxygen demand, chemical oxygen demand, nitrate, ammonia, and phosphate, i.e., 73%, 70%, 81%, 88%, and 67%, respectively. This setup also showed the best performance in treating the total coliforms, fecal coliforms, and E. coli with removal efficiency of 80%, 85%, and 83%, respectively, at 48 h HRT. © 2023, The Author(s) under exclusive licence to Iranian Society of Environmentalists (IRSEN) and Science and Research Branch, Islamic Azad University.
Juvenile polyposis syndrome
(2008) Vijai Upadhyaya; A. Gangopadhyaya; S. Sharma; S. Gopal; D. Gupta; Vijayendra Kumar
Aim: Report of a series of 12 cases of juvenile polyposis coli. Methods: The study period was from 1995 to 2005. All the patients were treated by total colectomy with rectal mucosectomy and endorectal ileoanal pullthrough with or without ileal pouch formation. Covering ileostomy was avoided in all the cases. Time taken for the surgery, postoperative complications and continence were documented. Results: The mean operating time was 4.2 h (range: 4-5 h). The mean duration of hospital stay was 16.3 days (range: 15-18 days). The most common postoperative complication was pouchitis and perianal excoriation. Initially, all the patients were passing stools at an interval of 2 h, and after 3 weeks, the frequency has reduced to 6-8 stools per day. In the follow-up after 3 months, the frequency was 3-5 per day with minimal soiling. Conclusions: Single-stage total colectomy with rectal mucosectomy and endorectal ileoanal pull-through without covering ileostomy and pouch formation is a safe and definitive treatment for juvenile polyposis coli if the patient selection is appropriate.
Single system ectopic ureter in females: A single center study
(2007) A. Gangopadhyaya; Vijay Upadhyaya; Anand Pandey; D. Gupta; S. Gopal; S. Sharma; Vijayendra Kumar
The purpose of this study was to inquire into the clinical features and methods for the diagnosis and management of single-system ectopic ureters associated with renal dysplasia. Materials and Methods: A total of 13 female patients were studied. Main stay of diagnosis was ultrasonography of KUB region and intravenous urography and renal scan was used to confirm the diagnosis. Histopathological evaluation was done in all cases for documentation of renal dysplasia. Result: In eight cases ectopic ureter with dysplastic kidney was seen on left side and in five it was on right side. All the patients were treated with nephroureterectomy of the affected side because of poor functioning of ipsilateral dysplastic kidney. Conclusion: Continuous urinary incontinence in females with a normal voiding pattern should prompt an evaluation for ureteric ectopia and when initial evaluation yields diagnosis of solitary kidney the clinician should be aware of the possibility of a hypoplastic and/or dysplastic on one side and normally functioning kidney on opposite side. Nephroureterectomy is the treatment of choice for unilateral single system ectopic ureter with renal dysplasia of affected side.
Surgical considerations in pediatric necrotizing fasciitis
(2009) A. Pandey; A.N. Gangopadhyay; S. Sharma; V. Kumar; S. Gopal; D. Gupta
Background: Necrotizing fasciitis (NF) is a serious infection of soft tissues. This paper presents experience with pediatric NF and suitability of conservative surgery in its management. Materials and Methods : In this retrospective study, 70 patients of NF were managed during the study period of eight years. The study was divided into two time periods- first period (June 1998 to June 2001- group 1) and second period (June 2001 to June 2006- group 2). The parameters studied were age, sex, site of involvement and treatment. The treatment included intravenous antibiotics, supportive therapy and either aggressive (group 1) or conservative surgery (group 2). Results: Age of presentation ranged from 10 days to 11 years. Male to female ratio was 1.69:1. Back was the commonest site to be involved. Culture reports were polymicrobial in 70% with predominance of Staphylococcus species. Predisposing factors included malnourishment, boils, scratch injury, intravenous cannulation and injections. Conservative surgery had better outcome in terms of hospital stay, complications and cost of treatment. Conclusion: NF is a serious and disease which requires immediate and all out attention. Early diagnosis, aggressive supportive treatment and conservative surgery improve survival.
Tetracycline as vital stain in the study of bone bio-dynamics
(1980) D. Gupta; S.M. Tuli
Unlike soft tissues, bone exhibits a biological record. The past events both physiological and pathological are recorded in a unique symbology on a durable tablet. All major physiological departures from the steady state, leave imprinted evidence for 15 to 20 years. Systemically administered tetracycline gets deposited wherever fresh mineralisation is taking place such as Haversian systems, cancellous trabeculae, subperiosteal surfaces, and endosteal borders. Under ultraviolet light it emits fluorescence which is woolly in appearance in woven bone and in areas of enchondral ossification. In the region of lamellar bone it appears as sharp bands. The tetracycline fluorescence serves as a label in time (provided one knows when the drug was used) and a label of morphology of newly forming bone. A dose of tetracycline at 30 mg per kg body weight given for 3 days, followed by an interval of 10 days till the next dose, as used in our experiments did not seem to be toxic to 'osteoneogenesis'. Preservation of specimens in 5 % formalin up to 7 days did not affect the fluorescence. It is only the multiply banded tetracycline labelling as vital stain which is more useful for study of bone biodynamics while single banded label can serve only as an indicator of accessibility of bone by circulation. Although one may not be able to translate all the symbology, however, with suitable precautions tetracycline fluorescence is one of the simpler, less expensive and reliable methods of studying bone biodynamics in vivo.
The bridging of large osteoperiosteal gaps using 'Decalbone'
(Springer-Verlag, 1988) S.M. Tuli; T.P. Srivastava; S.V. Sharma; S.C. Goel; D. Gupta; S. Khanna
'Decalbone' was prepared by partial decalcification of human bones obtained from recently amputated specimens. It was then stored in 80 to 90% ethanol in a domestic refrigerator. The decalbone was used to fill large osteoperiosteal gaps in 25 patients. The commonest lesion was a giant cell tumour (21 cases) and various long bones were affected. There were 6 failures with recurrence of the tumour in 3 and uncontrolled infection in 3. The remaining 19 cases were followed up for from 2 1/2 to 7 years. In 10 the decalbone incorporated well, but further reconstructive procedures were needed in 9. Our studies showed that incorporation began at around 6 to 9 months and was complete at about 2 years in the upper limb and 4 years in the lower limb. There was no clinical evidence of an immune response. © 1988 Springer-Verlag.
Unusual presentations in gout
(1980) V. Mohan; S.K. Gupta; I. Singh; D. Gupta
[No abstract available]