Browsing by Author "Debasis Mitra"

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Bioengineering Bacillus spp. for Sustainable Crop Production: Recent Advances and Resources for Biotechnological Applications
(Springer, 2025) Aurelio Ortiz; Estibaliz Sansinenea; Keswani Chetan; Tatiana Mikhailovna Minkina; Satyendra Pratap Singh; Bhagwan Narayan Rekadwad; Rainer Borriss; Kathleen Laura Hefferon; Trinh Xuan Hoat; Debasis Mitra; Pradeep Kumar Das Mohapatra; Periyasamy Panneerselvam
The goal of sustainable agriculture is to meet the rising need for food, while minimizing adverse impacts on the environment, protecting natural resources, and ensuring agricultural output over the long term. The pressing need to increase agricultural yield through sustainable agriculture is being emphasized. Several Bacillus species have been used as commercial biopesticides since they can act against plant pathogens by potentially suppressing them. At the same time, they can act as plant growth-promoting rhizobacteria and are known for their diverse characteristics and beneficial properties, making them potential candidates for use sustainable crop production programs. Knowledge of genetic information opens the door of possibility for understanding the way these microorganisms behave. By applying biotechnological tools to Bacillus, strategies can be adopted for the purpose of increasing the yield of crops and managing pests and pathogens that infect them. In this review, we identify the genes in the most significant Bacillus spp. that contribute to plant improvement. The most important biotechnological tools and advance computational approaches are described to provide an extended vision on this topic. However, increasing the crop production through application of beneficial microbial strains requires a multifaceted approach that considers ecological, economic, and social aspects. By implementing these strategies and practices, we can work towards a sustainable and resilient agricultural system that meets the growing food demand, while preserving the environment for future generations. © The Author(s) 2024.
Bioengineering Bacillus spp. for Sustainable Crop Production: Recent Advances and Resources for Biotechnological Applications
(Springer, 2024) Aurelio Ortiz; Estibaliz Sansinenea; Chetan Keswani; Tatiana Minkina; Satyendra Pratap Singh; Bhagwan Rekadwad; Rainer Borriss; Kathleen Hefferon; Trinh Xuan Hoat; Debasis Mitra; Pradeep Kumar Das Mohapatra; Periyasamy Panneerselvam
The goal of sustainable agriculture is to meet the rising need for food, while minimizing adverse impacts on the environment, protecting natural resources, and ensuring agricultural output over the long term. The pressing need to increase agricultural yield through sustainable agriculture is being emphasized. Several Bacillus species have been used as commercial biopesticides since they can act against plant pathogens by potentially suppressing them. At the same time, they can act as plant growth-promoting rhizobacteria and are known for their diverse characteristics and beneficial properties, making them potential candidates for use sustainable crop production programs. Knowledge of genetic information opens the door of possibility for understanding the way these microorganisms behave. By applying biotechnological tools to Bacillus, strategies can be adopted for the purpose of increasing the yield of crops and managing pests and pathogens that infect them. In this review, we identify the genes in the most significant Bacillus spp. that contribute to plant improvement. The most important biotechnological tools and advance computational approaches are described to provide an extended vision on this topic. However, increasing the crop production through application of beneficial microbial strains requires a multifaceted approach that considers ecological, economic, and social aspects. By implementing these strategies and practices, we can work towards a sustainable and resilient agricultural system that meets the growing food demand, while preserving the environment for future generations. © The Author(s) 2024.
Insight into an Effective Development of Biocontrol Agent Formulation for Commercial Production
(Springer Science+Business Media, 2025) Swarnmala Samal; Rahul Kumar; Divya Gunsola; Apaarna; Debasis Mitra; Madhu Thapliyal; Subhankar Mondal; Bahman Khoshru; Magdalena M. Knezevic; R. D. Dodiya
Sustainable agriculture relies heavily on effective biocontrol agents (BCAs), which provide a greener substitute for conventional pesticides. The key elements in the creation and formulation of BCAs for commercial production were examined in this study. Important factors to consider include the choice of strong microbial strains or natural enemies, growth condition optimization, formulation techniques to improve stability and efficacy, and regulatory needs for commercial viability. The analysis of case studies and latest developments in BCA formulation technologies aims to emphasize the effective strategies and obstacles encountered while transitioning from lab prototypes to large-scale manufacturing. This study offers a thorough framework for developing BCA formulations for sustainable agricultural practices by combining knowledge from microbiology, agriculture, and biotechnology. © 2025 The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Nature Singapore Pte Ltd.
Isolation and identification of Rhizospheric and Endophytic Bacteria from Cucumber plants irrigated with wastewater: Exploring their roles in plant growth promotion and disease suppression
(Elsevier Ltd, 2024) Kumar Shreshtha; Satyam Raj; Arun Kumar Pal; Pooja Tripathi; Krishna Kumar Choudhary; Debasis Mitra; Anju Rani; Sergio de los Santos-Villalobos; Vijay Tripathi
Wastewater contains various emerging contaminants, including heavy metals, residues of pesticides, and pharmaceuticals. Therefore, irrigation with wastewater can enhance heavy metal contamination in soil and adversely affect plant growth. To mitigate this problem, plant growth-promoting bacteria (PGPR) can improve plant growth under heavy metal stress. This study aimed to isolate and characterize rhizospheric and endophytic bacteria from the rhizosphere soil and roots of a cucumber plant irrigated with municipal wastewater. A total of 121 morphologically distinct bacterial isolates from the rhizosphere and 90 bacterial isolates from the endophytic region were isolated and tested for heavy metal resistance and in vitro plant growth-promoting characteristics, including indole-3-acetic acid (IAA) production, phosphate solubilization, Hydrogen Cyanide (HCN) production, and siderophore production. Most of the bacteria analyzed from the rhizospheric and endophytic regions showed various plant growth-promoting characteristics and were tolerant to different heavy metals at various concentrations. Bacterial strains R1 (Proteus sp.) and E2 (Bacillus sp.) were antagonistic to Fusarium oxysporum f. sp. Lycopersici. Wastewater irrigation increases heavy metal-resistant bacteria in cucumber plants, which can alleviate heavy metal stress. Additionally, Proteus sp. and Bacillus sp. isolates are potential candidates for removing heavy metal-contaminated soil and could be potential biofertilizer candidates for selected plants and biocontrol agents. © 2024 The Author(s)