Browsing by Author "Deborshi Sharma"

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Apoptosis: A basic physiologic process in wound healing
(2005) Nirendra K. Rai; Kamlakar Tripathi; Deborshi Sharma; Vijay K. Shukla
Apoptosis, or programmed cell death, is a complex network of biochemical pathways for controlling such events in a cell. Apoptosis is essential, as its failure can lead to disease. Because apoptosis concerns the regulation of sequential events, including the removal of inflammatory cells and the evolution of granulation tissue into scar tissue, it has an essential role in wound repair. This article examines the literature and proposes that apoptosis features in the development of diabetic foot wounds. Hyperglycemia deregulates the sequential apoptotic events by multiple mechanisms, leading to delayed wound healing. Deregulated apoptosis is emerging as a prominent cause of delayed wound healing, especially in diabetic wounds, along with the well-known triad of peripheral vascular disease, neuropathy, and infection. © 2005 Sage Publications.
Arginase activity in carcinoma of the gallbladder: A pilot study
(2009) Vijay Kumar Shukla; Ashutosh Tandon; Braja Kishor Ratha; Deborshi Sharma; Tej Bali Singh; Somprakas Basu
Carcinoma of the gallbladder is the third most common cancer of the gastrointestinal tract. Recent studies have shown increased arginase activity in various malignancies. The main aim of this study was to evaluate whether arginase activity increases in carcinoma of the gallbladder. The arginase activity was evaluated in serum and gallbladder tissue in 22 patients with histologically proven carcinoma of the gallbladder and 20 patients with cholecystitis using spectrophotometry and western blot assay. The Student's t-test, analysis of variance, and Student-Newman-Keuls test were used for comparison of data and for statistical significance. The mean tissue arginase and serum arginase activity (118.64±17.45 and 15.91±1.91, respectively) in cases of carcinoma of the gallbladder were significantly higher in comparison with cholecystitis (86.37±4.45 and 12.73±0.72, respectively). Subgroup analysis showed stage III gallbladder carcinoma had the maximum tissue arginase activity (142.00±21.68 U/g of tissue) followed by stage II (124.15±19.88) and stage I (108.46±6.73). This significant rise in mean tissue arginase and serum arginase activity in patients with gallbladder cancer probably supports an association between arginase activity and the malignancy. © 2009 Lippincott Williams & Wilkins, Inc.
Diagnostic value of serum CA242, CA 19-9, CA 15-3 and CA 125 in patients with carcinoma of the gallbladder.
(2006) Vijay K Shukla; Gurubachan; Deborshi Sharma; V.K. Dixit; Usha
BACKGROUND: Tumor markers have an increasing significance in the diagnosis and evaluation of tumor, but their role in gallbladder cancer has not been established. The present study was undertaken to determine the utility of serological markers in carcinoma of the gallbladder (CaGB). METHODS: This study was carried out in 55 cases and 8 healthy controls presenting to a single surgical unit of the University Hospital, Varanasi, India. CA242, CA19-9, CA15-3 and CA125 were assayed preoperatively in serum of patients with carcinoma of the gallbladder (39), cholelithiasis (16) and healthy controls (8) using ELISA technique. RESULTS: Mean concentration of all tumor markers was significantly raised in carcinoma of the gallbladder when compared with cholelithiasis. CA 242 was 12.10 vs 42.19 u/ ml, CA19-9 was 211.27 vs 86.06 uml, CA 15-3 was 71.42 vs 1.93u/ml and CA125 was 253.61 vs 65.5 u/ml <0.05). Sensitivity and specificity were calculated at various cut off points. Significant changes in CAl9-9 and CA242 occurred with advanced stage (p <0.05) and grade of tumor (p<0.00 1). When two tumor markers were combined, like CA242 and CA125, sensitivity and specificity improved to 87.5% and 85.7% respectively. Diagnostic accuracy is highest with a combination of CA 19-9 and CA 125 (80.65%). However, combination of tumor markers did not improve any further sensitivity or specificity of markers. CONCLUSION: Assay of CA242, CA15-3, CA19-9 and CA 125 are fairly good markers for discriminating patients of carcinoma of the gallbladder from cholelithiasis. CA242 and CA125 when used together achieved best sensitivity and specificity. Serum markers seem to be less sensitive when used individually in carcinoma of the gallbladder but may prove useful in combination.
Electrophoretic pattern of proteins in carcinoma of the gallbladder
(2008) Vijay K. Shukla; Sachin Goel; Sunil K. Trigun; Deborshi Sharma
Carcinoma of the gallbladder is a common health problem in the northern region of India and in spite of improved diagnostic techniques it is generally diagnosed at an advanced stage. This study was carried out in 60 patients, 30 of whom were diagnosed with gallbladder carcinoma and 30 with cholelithiasis. The protein content in tissue extracts and serum was determined using the Folin method of Lowry. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis was performed for the identification of proteins according to Laemmli. The results showed the mean concentration of total protein in gallbladder tissue of patients with carcinoma of the gallbladder was 51.83±3.36 mg/g tissue (45.33-57.80) and in patients with cholelithiasis it was 38.82±9.11 mg/g tissue (29.55-50.99) (P<0.001). Protein electrophoresis of gallbladder tissue from the patients with carcinoma of the gallbladder showed three additional bands of protein (two protein bands were present in the region of 50-55 kDa and the third band was present in the region of 35 kDa), which were absent in the gallbladder tissue of cholelithiasis patients. Mean total protein content in serum was 72.2±1.73 g/l (67.98-74.99) in patients of carcinoma of the gallbladder, whereas it was 71.01±3.4 g/l (60.00-78.99) (P>0.05) in the patients with cholelithiasis. Electrophoretic analysis of serum protein revealed at least two additional protein bands in patients with carcinoma of the gallbladder as compared with electrophoretic pattern in cholelithiasis. These two bands were in the range of 80 and 25 kDa proteins. In conclusion, the presence of these new bands of protein in the gallbladder tissue and serum of the patients with carcinoma of the gallbladder indicate their role in the pathogenesis of the carcinoma of the gallbladder. © 2008 Lippincott Williams & Wilkins, Inc.
Laparoscopic splenectomy: 16 years since delaitre with review of current literature
(2009) Deborshi Sharma; Vijay K. Shukla
Since Delaitre reported his first laparoscopic splenectomy it has been accepted universally and today it is probably regarded as a Gold standard in removal of benign and normal sized spleens. We discuss the indications, advantages, techniques, and recent advances of Laparoscopic splenectomy after 16 years of Delaitre. Copyright © 2009 by Lippincott Williams & Wilkins.
Malignant transformation of a pilonidal sinus
(2009) Deborshi Sharma; Arvind Pratap; Amrita Ghosh; Vijay K. Shukla
[No abstract available]
Primary angiosarcoma of the breast
(2007) Satyendra K. Tiwary; Manish K. Singh; Rahul Prasad; Deborshi Sharma; Mohan Kumar; Vijay K. Shukla
[No abstract available]
Primary rectal teratoma
(2008) Deborshi Sharma; Sanjeev Kumar; Ashutosh Tandon; Amrita Ghosh Kar; Mohan Kumar; V.K. Shukla
[No abstract available]
Retrorectal dermoid cyst in an adult
(2008) Deborshi Sharma; R. Nandini; Deepali Goel; Amrita Ghosh; Ram C. Shukla; Vijay K. Shukla
[No abstract available]
Unconsidered cause of bowel obstruction--gossypiboma.
(2008) Deborshi Sharma; Arvind Pratap; Ashutosh Tandon; Ram Chandra Shukla; Vijay K Shukla
[No abstract available]