Browsing by Author "Deepanshu Verma"

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Advancement of Single-Cell Sequencing in Medulloblastoma
(Humana Press Inc., 2022) Deepanshu Verma; Namyashree Nayak; Ashuthosh Singh; Ashutosh Kumar Singh; Neha Garg
Single-cell sequencing is a promising attempt to investigate the genomic, transcriptomic, and multiomic level of individual cell in the larger population of cells. The outward evolution of the technique from a manual method to the automation of single-cell sequencing is cogent. Lately, single-cell sequencing is widely used in various fields of science and has applications in neurobiology, immunity, cancer, microbiology, reproduction, and digestion. This chapter introduces the reader to the details of single-cell sequencing, currently used in several small-scale and commercial platforms. The advancement of single-cell sequencing in brain cancer sheds light on questions unanswered so far in the field of oncology. © 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
Indian medicinal plants as drug leads in neurodegenerative disorders
(Elsevier, 2020) Rohit Sharma; Neha Garg; Deepanshu Verma; Preeti Rathi; Vineet Sharma; Kamil Kuca; Pradeep Kumar Prajapati
There is rising global interest toward herbal or traditional medicines due to their natural origin and lesser side effects than conventional synthetic drugs that have been described with unwanted but unavoidable side effects. Therefore herbal therapies are being preferred over conventional treatments as an effective remedy of many brain disorders. Ancient system of medicine-Ayurveda-is widely practiced in Indian subcontinent with a rich traditional wisdom of nootropic herbs known for their multidimensional utility in various conditions. Strewn information is available relating to role of Indian traditional herbs for various brain disorders. Present review includes: (i) common neurodegenerative brain disorders with the associated changes, (ii) important Ayurvedic botanicals having potential roles in neurodegenerative disorders with description of their traditional usage, administration, and possible mechanism of actions. © 2021 Elsevier Inc.
Microbiome and host crosstalk: A new paradigm to cancer therapy
(Academic Press, 2021) Ashutosh Singh; Namyashree Nayak; Preeti Rathi; Deepanshu Verma; Rohit Sharma; Ashun Chaudhary; Alka Agarwal; Yamini Bhushan Tripathi; Neha Garg
The commensal microbiome of humans has co-evolved for thousands of years. The microbiome regulates human health and is also linked to several diseases, including cancer. The advances in next-generation sequencing have significantly contributed to our understanding of the microbiome and its association with cancer and cancer therapy. Recent studies have highlighted a close relationship of the microbiome to the pharmacological effect of chemotherapy and immunotherapy. The chemo-drugs usually interfere with the host immune system and reduces the microbiome diversity inside the body, which in turn leads to decreased efficacy of these drugs. The human microbiome, specifically the gut microbiome, increases the potency of chemo-drugs through metabolism, enzymatic degradation, ecological differences, and immunomodulation. Recent research exploits the involvement of microbiome to shape the efficacy and decrease the toxicity of these chemo-drugs. In this review, we have highlighted the recent development in understanding the relationship of the human microbiome with cancer and also emphasize on various roles of the microbiome in the modulation of cancer therapy. Additionally, we also summarize the ongoing research focussed on the improved efficacy of chemotherapy and immunotherapy using the host microbiome. © 2020 Elsevier Ltd
Microbiome for Personalized Medicine
(Springer Singapore, 2020) Preeti Rathi; Deepanshu Verma; Ashutosh Singh; Neha Garg
In recent years, microbiome has attracted much attention due to its role in host homeostasis and disease. Based on the microbiota of an individual, the microbiome can act as the biomarker as well as aid to personalized medicine, therefore reducing the adverse effects and cost of the medications associated. Comparing the microbiome profile of the diseased and the healthy individual and correlating with the bacteria causing the disease can provide clues regarding the associated biomarkers. Re-establishing the microbiome via microbiota transfer, diet, or medicine can act as personalized medicine for the individual. Due to the dearth of culturable properties of various microbial communities, advancement in the sequencing technologies has opened new doors in the microbiome world. In this chapter, we have emphasized the role of microbiome/microbiota with personalized medicine in various diseases like cancer, liver disorders, diabetes, and obesity. © Springer Nature Singapore Pte Ltd. 2020.
Quercetin acts as a P-gp modulator via impeding signal transduction from nucleotide-binding domain to transmembrane domain
(Taylor and Francis Ltd., 2022) Ashutosh Singh; Sandesh Kumar Patel; Prateek Kumar; Kanhu Charan Das; Deepanshu Verma; Rohit Sharma; Timir Tripathi; Rajanish Giri; Natália Martins; Neha Garg
The inherent ability of the cancer cells to resist chemotherapeutic agents is a major challenge in drug discovery. Chemotherapy is one of the most widely used treatment methods for cancer, but due to multidrug resistance (MDR) development in cancer cells, the healing procedure often fails. Various factors impart cancer resistance to cells; among them, P-glycoprotein (P-gp) overexpression is directly linked to MDR in cancer cells. P-gp leads to the efflux of drug molecules to the extracellular space. Several molecules have been reported to inhibit the P-gp activity. Among them, quercetin has revealed a great potential to modulate P-gp activity. However, the mechanistic understanding of quercetin induced modulation is not entirely elucidated. In the present work, we showed that quercetin binds in the interacting region between the transmembrane domain and nucleotide-binding domain out of the three plausible binding sites of P-gp and restrict the conformational change from inward- to outward-facing conformation of P-gp. Due to the absence of the inward-facing structure of human P-gp, we first modeled an inward-facing P-gp structure. Using molecular docking, the interacting residues of P-gp were identified, and the stability and interaction dynamics of the complex were studied using molecular dynamics simulation. Our work reveals the mechanistic understanding of quercetin induced modulation of P-gp and indicates its importance in cancer treatment. Communicated by Ramaswamy H. Sarma. © 2020 Informa UK Limited, trading as Taylor & Francis Group.