Browsing by Author "Deepjyoti Singh"

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Green synthesis of silver nanoparticles by root extract of Premna integrifolia L. and evaluation of its cytotoxic and antibacterial activity
(Elsevier Ltd, 2023) Chandrashekhar Singh; Sumit Kumar Anand; Richa Upadhyay; Nidhi Pandey; Pradeep Kumar; Deepjyoti Singh; Punit Tiwari; Rajesh Saini; Kavindra Nath Tiwari; Sunil Kumar Mishra; Ragini Tilak
An efficient, reproducible, and eco-friendly protocol has been developed for the green synthesis of silver nanoparticles using an aqueous root extract of Premna integrifolia (PE-AgNPs). The synthesized nanoparticles were characterized by different techniques to determine their formation, stability, and morphology. Nanoparticles were tested for cytotoxicity against the HepG2 cancer cell line and antibacterial activity against clinical isolates of bacteria. The cytotoxic potential of PE-AgNPs was analyzed using MTT assay, Hoechst and AO/EtBr staining, ROS measurement, mitochondrial membrane potential, clonogenic, and wound healing assays. PE-AgNPs were found strongly cytotoxic against Hep G2 cell lines during all assays. A disc diffusion assay confirmed that Pseudomonas aeruginosa was most susceptible to PE-AgNPs than Staphylococcus aureus and E. coli. It was further confirmed by MIC, MBC values, and TEM microphotographs. As a result, our findings indicate that biosynthesized silver nanoparticles could be useful as cytotoxic and antimicrobial candidates. © 2023 Elsevier B.V.
Soil Algae as a Feedstock for Biofuel Production and Value-Added Products
(Springer Nature, 2025) Vijayata Singh; Swati Mishra; Deepjyoti Singh
Microalgae are a sustainable and economical source of biofuels and other valuable products. Algae are autotrophic organisms that can survive in aquatic or terrestrial forms. They are fast-growing microorganisms with a high potential for converting CO2 from the atmosphere into valuable products such as polysaccharides, fats, and other bioactive metabolites. Fuel derived from algae is named “third-generation biofuel,” which has several critical positives over plant-based (first) and animal waste feedstock (second). Biofuel companies are currently seeking to increase the commercial algal product and pursuing several engineering and biotechnological approaches to design an economic system for proper algal growth. However, microalgal research and production are mainly confined to aquatic forms, with specific limitations like water contamination and the high cost of dewatering feedstock during processing. Overexploitation of aquatic algae also leads to ecosystem imbalance as aquatic algae are the primary producers. Soil algae can be used as an alternative to counterbalance the usage of aquatic algae. The present chapter compiles some terrestrial algae that can be used as potential biomass to produce biofuels and various bioactive products. © The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Nature Singapore Pte Ltd. 2025.