Browsing by Author "Devendra Kumar Choudhary"

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Comparative expression analysis of defence-related genes in Bacillus-treated Glycine max upon challenge inoculation with selective fungal phytopathogens
(Indian Academy of Sciences, 2018) Shekhar Jain; Anukool Vaishnav; Ajit Varma; Devendra Kumar Choudhary
Activation of defence-related genes by the application of beneficial bacteria leads to prior protection against pathogens through induced systemic resistance. The present study was carried out to examine the qRT- PCR-based relative quantification of differently expressed defence-related genes in soybean (Glycine max L. Merrill) plants primed with Bacillus sp. strain SJ-5 against the fungal pathogen Rhizoctonia solani and Fusarium oxysporum. In this context, molecular characterization of plant growth promoting and biocontrol genes of SJ-5 was done by PCR followed by homology analysis. In the GC-MS analysis of SJ-5 volatile organic compounds, potent antifungal compound bis(2-ethylhexyl) phthalate and antioxidant compound butylated hydroxy toluene were reported with the highest peak area 47.96% and 21.82% respectively, along with other antifungal compounds in small proportion. Qualitative expression of different defence-related genes like lipoxygenase, phenylalanine ammonia-lyase 2, peroxidase, polyphenol oxidase, endo-1,3-beta-glucanase, catalase, defensin-like protein, vegetative storage protein and chitinase class I was found elicited in the plants primed with SJ-5 against the fungal pathogens. In the qPCR analysis, the highest upregulation was observed in the transcript profile of ppojh2 in the treatments T5 and T6 with 4.12- and 4.06-fold increase respectively. © 2018 Current Science Association, Bengaluru.
Regulation of Drought-Responsive Gene Expression in Glycine max L. Merrill is Mediated Through Pseudomonas simiae Strain AU
(Springer New York LLC, 2019) Anukool Vaishnav; Devendra Kumar Choudhary
Plant growth promoting rhizobacteria (PGPR) have been described for sustainable agriculture practices as being a vital agent for abiotic and biotic stress mitigation and growth promotion in plants. In the present research, the authors emphasize the role of drought tolerant PGPR namely, Pseudomonas simiae strain AU, in protection of soybean plants by modulating the gene expression profile and phytohormone biosynthesis responsible for drought tolerance in plants. The gene expression analysis confirmed the involvement of transcription factors (DREB/EREB), osmoprotectants (P5CS, GOLS), and water transporters (PIP & TIP), as these genes were up-regulated in P. simiae AU-inoculated plants leading to drought tolerance. In addition, enhanced production of abscisic acid (ABA) and salicylic acid (SA) hormones and reduction of ethylene emission, associated with promoting drought tolerance, was observed in bacterial-inoculated plants in comparison to non-inoculated plants. Higher proline and total soluble sugar contents in AU-inoculated soybean plants also contributed to increased tolerance to drought stress. Overall, P. simiae AU mediated drought-induced expression profiles of stress genes and plant hormones were determined in soybean plants. © 2018, Springer Science+Business Media, LLC, part of Springer Nature.