Browsing by Author "Diksha Joshi"

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ENDOPHYTIC BACTERIAL STRAINS FROM CURCUMA AMADA RHIZOME: BIOCHEMICAL CHARACTERIZATION AND PLANT GROWTH PROMOTING TRAITS ANALYSIS
(Prof (Dr.) P. R. Yadav, 2025) Diksha Joshi; Monika M. Singh; Rahul Prasad Singh; Priyvrat Singh Chauhan; Nikunaj Bhardwaj; Ajay Kumar
Curcuma amada Roxb. commonly referred as mango ginger is a promising herb, frequently used in the traditional medical system due to their broad pharmacological activity. In this study, endophytic bacterial strains were isolated from rhizome of Curcuma amada. All these endophytic bacterial strains were characterized and designated as Bacillus species CA1, Bacillus subtilis CA2, Pseudomonas aeruginosa CA11 and Pseudomonas sp. CA13 on the basis of biochemical or molecular rRNA gene sequence analysis. All these four strains showed some differential response during the biochemical test and plant growth promoting traits analysis. All strains showed positive response during the Catalase, Glucose, Sucrose and Starch hydrolysis test. However, for the Maltose, Mannose strains showed differential response. The strain P. aeruginosa CA11 synthesized higher amount (42.34±2.67 µg ml-1) of IAA production while minimum was recorded in B. subtilis (32.65 µg ml-1). All the strains showed phosphate solublization and siderophore activity. Similarly, during salinity stress tolerance test, P. aeruginosa CA11 showed highest tolerance of 8% and Bacillus sp. showed lowest of 5% of NaCl level. The study showed that these strains especially P. aeruginosa CA11 can be used as microbial biofertlizers during the normal and salinity stress conditions. © 2025, Prof (Dr.) P. R. Yadav. All rights reserved.
Mechanisms of Stress Tolerance in Cyanobacteria under Extreme Conditions
(Multidisciplinary Digital Publishing Institute (MDPI), 2022) Priya Yadav; Rahul Prasad Singh; Shashank Rana; Diksha Joshi; Dharmendra Kumar; Nikunj Bhardwaj; Rajan Kumar Gupta; Ajay Kumar
Cyanobacteria are oxygen-evolving photoautotrophs with worldwide distribution in every possible habitat, and they account for half of the global primary productivity. Because of their ability to thrive in a hostile environment, cyanobacteria are categorized as “extremophiles”. They have evolved a fascinating repository of distinct secondary metabolites and biomolecules to promote their development and survival in various habitats, including severe conditions. However, developing new proteins/enzymes and metabolites is mostly directed by an appropriate gene regulation system that results in stress adaptations. However, only few proteins have been characterized to date that have the potential to improve resistance against abiotic stresses. As a result, studying environmental stress responses to post-genomic analysis, such as proteome changes using latest structural proteomics and synthetic biology techniques, is critical. In this regard, scientists working on these topics will benefit greatly from the stress of proteomics research. Progress in these disciplines will aid in understanding cyanobacteria’s physiology, biochemical, and metabolic systems. This review summarizes the most recent key findings of cyanobacterial proteome study under various abiotic stresses and the application of secondary metabolites formed during different abiotic conditions. © 2022 by the authors.