Browsing by Author "Dinesh Chandra Joshi"

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A consortium of arbuscular mycorrizal fungi improves nutrient uptake, biochemical response, nodulation and growth of the pea (Pisum sativum L.) under salt stress
(Elsevier B.V., 2020) Manoj Parihar; Amitava Rakshit; Kiran Rana; Rajendra Prasad Meena; Dinesh Chandra Joshi
The present study was conducted to examine the accumulation of nutrients, antioxidant enzymes activity, growth and yield of pea influenced by arbuscular mycorrhizal fungi (AMF) under salt stress. This study included four treatments i.e. single species AMF (Rhizophagus intraradices), formulated AMF (Funneliformis mosseae and Rhizophagus intraradices), and multi species AMF (Rhizophagus fasciculatus and Gigaspora sp.) along with control (non-mycorrhizal). The AMF inoculation found to be effective in reducing the negative effects of salt stress by improving the antioxidant enzyme system, greater ionic balance, and by regulating the malondialdehyde and phenolic compounds, which resulted in higher growth and yield of pea. Seed yield increased by ~24, 40 and 54% in Rhizophagus intraradices, Funneliformis mosseae + Rhizophagus intraradices and Rhizophagus fasciculatus + Gigaspora sp. respectively in comparison to control treatments. Overall, consortium based application of AMF belonging to different family i.e. multi species (Rhizophagus fasciculatus and Gigaspora sp.) was found superior than single AMF inoculation to improve the pea production under salt stress environment. © 2020 Elsevier B.V.
Identification and characterization of Eco-miR 169-EcNF-YA13 gene regulatory network reveal their role in conferring tolerance to dehydration and salinity stress in finger millet
(Nature Research, 2025) Varsha Rani; Sumi Rana; Mehanathan Muthamilarasan; Dinesh Chandra Joshi; Ramwant Kumar Gupta; Rajesh Kumar Singh; D. L. Yadav
The finger millet (Eleusine coracana (L.) Gaertn) genome, comprised 166 conserved microRNAs (miRNAs) belonging to 39 families and three novel miRNAs. The miR169 is one of the most conserved miRNA families, while Eco_N1 is a species-specific miRNA prevalent in finger millet. Its members regulate the expression of genes encoding the Nuclear Factor-Y subunit A (NF-YA) via transcript cleavage. However, the role of miRNA genes in regulating the expression of NF-YA transcription factors in finger millet needs to be deciphered. The present study characterized 166 conserved and novel miRNAs (Eco_N1, Eco_N2 and Eco_N3). Further, secondary structures were predicted, and the potential miR genes targeting the NF-YA transcription factors regulating abiotic stress tolerance were analysed. Twenty-three Eco-miR169 members and one Eco_N1 miRNA targeting EcNF-YA13 were identified in the finger millet genome. The presence of relevant cis-elements such as ABRE (abscisic acid-responsive elements), DRE (dehydration-responsive element), and MYB (myeloblastosis) indicates that the target of Eco-miR169 might be involved in abiotic stress responses. The tissue-specific RNA-seq transcriptomic expression pattern of Eco-miR169 showed variable fold of expression in seedlings compared to the control. At the same time, the expression of EcNF-YA13 (target genes of Eco-miR169 members and Eco_N1) presented a downregulated trend under salinity and dehydration conditions compared to the control. Tissue-specific RNA-seq followed by expression analysis confirmed the antagonistic effect of Eco-miR genes on EcNF-YA13. In a nutshell, the results of this study could be utilized as a platform for further exploration and characterization of finger millet Eco-miR169-EcNF-YA13gene regulatory network. © The Author(s) 2025.