Browsing by Author "Divya"

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Bimetallic Copper/Zinc Metal Organic Framework-MoS2Nanohybrid based Electrochemical Sensor
(Institute of Electrical and Electronics Engineers Inc., 2024) Divya; Shubhangi; Pranjal Chandra
Acetaminophen is a globally used antipyretic analgesic drug to relieve pain. The excess usage of acetaminophen leads to various health implications including cardiovascular ailments, asthma, liver and kidney damage. Bimetallic MOFs are emerging materials in the field of electrochemical sensing domain utilizing the synergistic effect of both the metal ions present within. In this study, we report a sensing matrix comprising of an electrochemically fabricated novel bimetallic MOF (CoZn) conjugated with Mos2nanosheets to form an electroconductive nanocomposite. Layer-by-layer characterization of CoZn-MOF/MoS2modified electrode surface was done through different electrochemical analytical techniques like CV and EIS. The composite (GCE/CoZn-MOF/MoS2) can find its applications in sensing a plethora of analytes based on the catalytic potential of the metal nodes. In this work we have attempted the application of the developed nanocomposite probe in the electrochemical oxidation and thereby detection of acetaminophen. The developed nanocomposite was able to detect acetaminophen with enhancement in signal, proving the improved electroconductivity of the surface due to synergistic effect of CoZn-MOF and Mos2. © 2024 IEEE.
Biochar for pollutants bioremediation from soil and water ecosystem
(Elsevier, 2024) Amit K. Dash; Saloni Tripathy; A. Naveenkumar; Tanmaya K. Bhoi; Arpna Kumari; Divya; Ashish M. Latare; Tony Manoj Kumar Nandipamu; Virendra Singh; Md. Basit Raza; Anuj Saraswat; Jehangir Bhadha
Emerging contaminants (ECs) are chemical compounds that have potentially hazardous effects, but risks associated with their exposure have not been fully explored and are not yet subject to regulation. The fact that these ECs pollute both soil and water has recently raised significant concerns. Human and natural factors together make up the main sources of soil contamination. ECs are difficult to remove from soil and the aqueous system since they are retained in the environment for a very long period. Several techniques, including biodegradation, photocatalytic degradation, nanofiltration, membrane bioreactors, separation, enhanced chemical oxidation, and others, have been developed to remove ECs from the environment. One of the well-known innovations for tackling the issue of ECs is the application of in-situ biochar. Biochar application can significantly reduce the bioavailability of ECs that accumulate in soil and/or aqueous systems. It also has the added advantage of soil fertilization and climate change mitigation. In this chapter, emphasis has been given to the use of biochar for the remediation of ECs from the soil and aqueous system and factors affecting the efficiency of biochar as a bioremediation technique. © 2024 by Elsevier Inc. All rights reserved, including those for text and data mining, AI training, and similar technologies.
Enhancing milk quality assessment with watermelon (Citrullus lanatus) urease immobilized on VS2-chitosan nanocomposite beads using response surface methodology
(Elsevier Ltd, 2024) Prince Kumar; Divya; Amit Kumar Patel; Anchal Srivastava; Arvind M. Kayastha
An eco-friendly hydrothermal method synthesized VS2 nanosheets. Several spectroscopic and microscopic approaches (TEM) were used to characterize the produced VS2 nanosheet microstructure. VS2, Chitosan, and nanocomposite were used to immobilize watermelon (Citrullus lanatus) urease. Optimization using the Response Surface Methodology and the Box-Behnken design yielded immobilization efficiencies of 65.23 %, 72.52 %, and 87.68 % for chitosan, VS2, and nanocomposite, respectively. The analysis of variance confirmed the mathematical model's validity, enabling additional research. AFM, SEM, FTIR, Fluorescence microscopy, and Cary Eclipse Fluorescence Spectrometer showed urease conjugation to the matrix. During and after immobilization, FTIR spectra showed a dynamic connectivity of chemical processes and bonding. The nanocomposite outperformed VS2 and chitosan in pH and temperature. Chitosan and VS2-immobilized urease were more thermally stable than soluble urease, but the nanocomposite-urease system was even more resilient. The nanocomposite retained 60 % of its residual activity after three months of storage. It retains 91.8 % of its initial activity after 12 reuse cycles. Nanocomposite-immobilized urease measured milk urea at 23.62 mg/dl. This result was compared favorably to the gold standard p-dimethylaminobenzaldehyde spectrophotometric result of 20 mg/dl. The linear range is 5 to 70 mg/dl, with a LOD of 1.07 (±0.05) mg/dl and SD of less than 5 %. The nanocomposite's ksel coefficient for interferents was exceptionally low (ksel < 0.07), indicating urea detection sensitivity. Watermelon urease is suitable for dairy sector applications due to its availability, immobilization on nanocomposite, and reuse. © 2024 Elsevier Ltd
Exploring the catalytic potential of watermelon urease: Purification, biochemical characterization, and heavy metal precipitation
(Elsevier B.V., 2024) Prince Kumar; Divya; Arvind M. Kayastha
Bioactive urease from watermelon (Citrullus lanatus) seeds was purified using acetone fractionation, anion-exchange, and size-exclusion chromatography, achieving a 121-fold increase and specific activity of 3216 U/mg. The enzyme appeared as a single band on native and SDS-PAGE, with a molecular mass of 480 ± 10 kDa and subunit mass of 80 ± 10 kDa, indicating six identical subunits. Atomic absorption spectroscopy revealed 1.46 nickel ions per subunit. Watermelon urease exhibited serological similarities with jack bean and pigeon pea ureases, an optimal pH of 7.3, an activation energy of 3 kcal/mol, Vmax of 3571 μmol/min/mg, and Km of 0.16 mM. The enzyme displayed biphasic thermal and pH inactivation kinetics, a strong preference for urea, and a half-life of 70 days with 1 mM DTT. This study highlights watermelon urease's role in bioremediation by facilitating the precipitation of heavy metals as stable carbonates, promoting environmental sustainability. © 2024 Elsevier B.V.
Ligand conjugated lipid-based nanocarriers for cancer theranostics
(John Wiley and Sons Inc, 2022) Rahul Kumar; Daphika S. Dkhar; Rohini Kumari; Divya; Supratim Mahapatra; Ananya Srivastava; Vikash Kumar Dubey; Pranjal Chandra
Cancer is one of the major health-related issues affecting the population worldwide and subsequently accounts for the second-largest death. Genetic and epigenetic modifications in oncogenes or tumor suppressor genes affect the regulatory systems that lead to the initiation and progression of cancer. Conventional methods, including chemotherapy/radiotherapy/appropriate combinational therapy and surgery, are being widely used for theranostics of cancer patients. Surgery is useful in treating localized tumors, but it is ineffective in treating metastatic tumors, which spread to other organs and result in a high recurrence rate and death. Also, the therapeutic application of free drugs is related to substantial issues such as poor absorption, solubility, bioavailability, high degradation rate, short shelf-life, and low therapeutic index. Therefore, these issues can be sorted out using nano lipid-based carriers (NLBCs) as promising drug delivery carriers. Still, at most, they fail to achieve site-targeted drug delivery and detection. This can be achieved by selecting a specific ligand/antibody for its cognate receptor molecule expressed on the surface of the cancer cells. In this review, we have mainly discussed the various types of ligands used to decorate NLBCs. A list of the ligands used to design nanocarriers to target malignant cells has been extensively undertaken. The approved ligand-decorated lipid-based nanomedicines with their clinical status have been explained in tabulated form to provide a wider scope to the readers regarding ligand-coupled NLBCs. © 2022 Wiley Periodicals LLC.
Shifting paradigm in electrochemical biosensing matrices comprising metal organic frameworks and their composites in disease diagnosis
(John Wiley and Sons Inc, 2024) Shubhangi; Divya; Sanjay K. Rai; Pranjal Chandra
Metal Organic Frameworks (MOFs) are an evolving category of crystalline microporous materials that have grabbed the research interest for quite some time due to their admirable physio-chemical properties and easy fabrication methods. Their enormous surface area can be a working ground for innumerable molecular adhesions and site for potential sensor matrices. They have been explored in the last decade for incorporation in electrochemical sensor matrices as diagnostic solutions for a plethora of diseases. This review emphasizes on some of the recent advancements in the area of MOF-based electrochemical biosensors with focus on various important diseases and their significance in upgrading the sensor performance. It summarizes MOF-based biosensors for monitoring biomarkers relevant to diabetes, viral and bacterial sepsis infections, neurological disorders, cardiovascular diseases, and cancer in a wide range of real matrices. The discussion has been supplemented with extensive tables elaborating recent trends in the field of MOF-composite probe fabrication strategies with their respective sensing parameters. The article sums up the future scope of these materials in the field of biosensors and enlightens the reader with recent trends for future research scope. This article is categorized under: Diagnostic Tools > Biosensing Diagnostic Tools > Diagnostic Nanodevices. © 2024 Wiley Periodicals LLC.