Browsing by Author "Divya Gupta"

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Application of essential oils for the production of dietary supplements and as traditional self-medication purposes
(Elsevier, 2023) Raghvendra Raman Mishra; Pragya Mishra; Subodh Kumar; Divya Gupta
Despite the fact that essential oils (EOs) have been studied for more than 60 years, interest in them has increased recently as more individuals look for natural remedies. Essential oils have been used in rituals and medicine for millennia because of their healing properties. This has existed since the Paleolithic era. Since they are more complex and contain a variety of volatile and naturally occurring bioactive substances, EOs are commonly used in the food sector as the finest alternatives. Despite the fact that EOs are difficult to use practically as an effective antibacterial due to their volatility, low solubility, and high instability, plant-based EOs have gained interest as a potential replacement for synthetic preservatives due to their environmental friendliness and widely acknowledged safety status. Among its traditional uses, the use of EOs in food products undoubtedly stands out. While there have been a number of recent reviews that have concentrated on the use and potential of EOs as food preservatives, there has been relatively less research on other functional roles of EOs or their components in food products, which are nevertheless generating significant public interest and a growing body of scientific evidence: their health-promoting role beyond preservation and basic nutrition. The present chapter points toward the use of EOs in the manufacturing of dietary supplements and traditional self-medication. © 2024 Elsevier Inc. All rights reserved.
Computational insights into diverse aspects of glutathione S-transferase gene family in Papaver somniferum
(Springer, 2022) Swati Vaish; Reshma Parveen; Rajneesh; Nootan Singh; Divya Gupta; Mahesh Kumar Basantani
Plant glutathione S-transferases are an ancient protein superfamily having antioxidant activity. These proteins are primarily involved in diverse plant functions such as plant growth and development, secondary metabolism, signaling pathways and defense against biotic and abiotic stresses. The current study aimed to comprehensively identify and characterize the GST gene family in the medicinally important crop Papaver somniferum. A total of 93 GST proteins were identified belonging to eight GST classes and found to be majorly localized in the cytoplasm. All GST genes were found on eleven opium chromosomes. Gene duplication analysis showed segmental duplication as a key factor for opium GST gene family expansion under strong purifying selection. Phylogenetic analysis with gymnosperm, angiosperm and bryophyte revealed the evolution of GSTs earlier than their division into separate groups and also prior to the divergence of monocot and dicot. The secondary structure prediction showed the dominance of α-helices indicative of PsomGSTs as structurally stable and elastic proteins. Gene architecture showed the conservation of number of exons across the classes. MEME analysis revealed only a few class specific and many across class conserved motifs. Ser was found to be the active site residue of tau, phi, theta and zeta class and Cys was catalytic residue of DHAR, lambda and GHR class. Promoter analyses identified many cis-acting regulatory elements related to hormonal, cellular, stress and light response functions. Ser was the key phosphorylation site. Only three glycosylation sites were found across the 93 PsomGSTs. 3D structure prediction was also performed and was validated. Interactome analyses revealed the correlation of PsomGSTs with glutathione metabolizing proteins. Gene enrichment analysis and KEGG pathway analyzed the involvement of PsomGSTs in three major pathways i.e. glutathione metabolism, tyrosine metabolism and ascorbate metabolism. The outcome revealed high model quality of PsomGSTs. The results of the current study will be of potential significance to understand the functional and structural importance of the GST gene family in opium, a medicinally important crop. © 2022, The Author(s) under exclusive licence to The Botanical Society of Japan.
Effects of Salt Stress on Osmolyte Metabolism of Crop Plants and Mitigating Strategy by Osmolyte
(wiley, 2021) Abreeq Fatima; Garima Singh; Anuradha Patel; Sanjesh Tiwari; Divya Gupta; Dilip Kumar Prajapati; Anurag Dubey; Sheo Mohan Prasad
Among various abiotic stresses, salinity is a major problem in arid and semiarid regions of the world that negatively reduces the crop productivity. Osmotic stress is the major cause of reduction in growth under salt stress and to minimize the osmotic stress, plants either accumulates or enhances the synthesis of osmoprotectants. The enhanced production of osmolytes is the manifestation of osmoprotectant responsive genes, which improves stress tolerance in plants. Most of the crop plants during abiotic stress conditions such as salinity, drought, and high temperature protect themselves through amino acid metabolism as well. Salinity is one of the most brutal environmental stresses that hamper plant growth and productivity worldwide. The biosynthetic pathways of major osmolyte such as proline, glycine betaine, polyamines, or some synthetic sugar molecules are severely affected under salt stress. Salt-induced toxicity is either alleviated by enhanced antioxidant system or by exogenous supplementation of these major osmolytes. © 2022 John Wiley & Sons Ltd. All rights reserved.
Salt Stress Toxicity Amelioration by Phytohormones, Synthetic Product, and Nutrient Amendment Practices
(wiley, 2021) Divya Gupta; Garima Singh; Sanjesh Tiwari; Anuradha Patel; Abreeq Fatima; Anurag Dubey; Neha Naaz; Jitendra Pandey; Sheo Mohan Prasad
This chapter discusses the various ameliorating approaches to cope up the loss of crop yield under saline environment. Phytohormones are essential growth regulators and are best known for their prominent impact on metabolism and stimulation of defense responses. The exogenous supplementation of plant growth hormones alleviates the salt toxicity and showing vital role in minimizing the stress by improving several metabolic processes like improving seed metabolism, maintaining the homeostasis redox reaction, and other physiological mechanisms. The exogenous supplementation or endogenous accumulation of proline which is an amino acid and a well-known product enhances behavior of salt-stressed plants. Nutrient management significantly improved the growth, physiological, and biochemical modifications as well as quality characteristics of the salt-stressed plants. Salt toxicity induced alterations in growth and development by inducing the ion toxicity, osmotic stress, essential nutrients deficiency, inhibition of electron transport chain associated with oxidative stress that limits the activation of anti-oxidative machinery, and water uptake from soil. © 2022 John Wiley & Sons Ltd. All rights reserved.