Browsing by Author "Durga P. Mishra"

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Anti-cancer evaluation of quercetin embedded PLA nanoparticles synthesized by emulsified nanoprecipitation
(Elsevier B.V., 2015) Sanjeev K. Pandey; Dinesh K. Patel; Ravi Thakur; Durga P. Mishra; Pralay Maiti; Chandana Haldar
This study was carried out to synthesize quercetin (Qt) embedded poly(lactic acid) (PLA) nanoparticles (PLA-Qt) and to evaluate anti-cancer efficacy of PLA-Qt by using human breast cancer cells. PLA-Qt were synthesized by using novel emulsified nanoprecipitation technique with varying dimension of 32 ± 8 to 152 ± 9. nm of PLA-Qt with 62 ± 3% (w/w) entrapment efficiency by varying the concentration of polymer, emulsifier, drug and preparation temperature. The dimension of PLA-Qt was measured through transmission electron microscopy indicating larger particle size at higher concentration of PLA. The release rate of Qt from PLA-Qt was found to be more sustained for larger particle dimension (152 ± 9. nm) as compared to smaller particle dimension (32 ± 8. nm). Interaction between Qt and PLA was verified through spectroscopic and calorimetric methods. Delayed diffusion and stronger interaction in PLA-Qt caused the sustained delivery of Qt from the polymer matrix. In vitro cytotoxicity study indicate the killing of ~50% breast cancer cells in two days at 100. μg/ml of drug concentration while the ~40% destruction of cells require 5 days for PLA-Qt (46 ± 6. nm; 20. mg/ml of PLA). Thus our results propose anticancer efficacy of PLA-Qt nanoparticles in terms of its sustained release kinetics revealing novel vehicle for the treatment of cancer. © 2015 Elsevier B.V.
Controlled release of drug and better bioavailability using poly(lactic acid-co-glycolic acid) nanoparticles
(Elsevier B.V., 2016) Sanjeev K. Pandey; Dinesh K. Patel; Akhilendra K. Maurya; Ravi Thakur; Durga P. Mishra; Manjula Vinayak; Chandana Haldar; Pralay Maiti
Tamoxifen (Tmx) embedded poly(lactic-co-glycolic acid) (PLGA) nanoparticles (PLGA-Tmx) is prepared to evaluate its better DNA cleavage potential, cytotoxicity using Dalton's lymphoma ascite (DLA) cells and MDA-MB231 breast cancer cells. PLGA-Tmx nanoparticles are prepared through emulsified nanoprecipitation technique with varying dimension of 17-30 nm by changing the concentrations of polymer, emulsifier and drug. Nanoparticles dimension are measured through electron and atomic force microscopy. Interactions between tamoxifen and PLGA are verified through spectroscopic and calorimetric methods. PLGA-Tmx shows excellent DNA cleavage potential as compared to pure Tmx raising better bioavailability. In vitro cytotoxicity studies indicate that PLGA-Tmx reduces DLA cells viability up to ~38% against ~15% in pure Tmx. Hoechst stain is used to detect apoptotic DLA cells through fluorescence imaging of nuclear fragmentation and condensation exhibiting significant increase of apoptosis (70%) in PLGA-Tmx vis-à-vis pure drug (58%). Enhanced DNA cleavage potential, nuclear fragmentation and condensation in apoptotic cells confirm greater bioavailability of PLGA-Tmx as compared to pure Tmx in terms of receptor mediated endocytosis. Hence, the sustained release kinetics of PLGA-Tmx nanoparticles shows much better anticancer efficacy through enhanced DNA cleavage potential and nuclear fragmentation and, thereby, reveal a novel vehicle for the treatment of cancer. © 2016 Elsevier B.V.
Corrigendum to “Controlled release of drug and better bioavailability using poly(lactic acid-co-glycolic acid) nanoparticles” [Int. J. Biol. Macromol. 89 (2016) 99–110] (S0141813016303737) (10.1016/j.ijbiomac.2016.04.065))
(Elsevier B.V., 2018) Sanjeev K. Pandey; Dinesh K. Patel; Akhilendra K. Maurya; Ravi Thakur; Durga P. Mishra; Manjula Vinayak; Chandana Haldar; Pralay Maiti
The authors regretfully submit that there was human mistake and it can be rectified by adding a corrigendum in which the following be mentioned: [Figure presented] Fig. 1a incorrect one be immediately replaced by the Fig. 1a correct one. The human mistake was due to folders having electron micrograph of all experiment and during transfer of images from technical person to us. The authors would like to apologies for inconvenience caused. © 2018 Elsevier B.V.