Browsing by Author "Durgesh Kumar Jaiswal"

Now showing 1 - 20 of 44

Advances and future prospects of pyrethroids: Toxicity and microbial degradation
(Elsevier B.V., 2022) Saurabh Singh; Arpan Mukherjee; Durgesh Kumar Jaiswal; Arthur Prudêncio de Araujo Pereira; Ram Prasad; Minaxi Sharma; Ramesh Chander Kuhad; Amritesh Chandra Shukla; Jay Prakash Verma
Pyrethroids are a class of insecticides structurally similar to that of natural pyrethrins. The application of pyrethrins in agriculture and pest control lead to many kinds of environmental pollution affecting human health and loss of soil microbial population that affect soil fertility and health. Natural pyrethrins have been used since ancient times as insect repellers, and their synthetic versions especially type 2 pyrethroids could be highly toxic to humans. PBO (Piperonyl butoxide) is known to enhance the toxicity of prallethrin in humans due to the resistance in its metabolic degradation. Pyrethroids are also known to cause plasma biochemical profile changes in humans and they also lead to the production of high levels of reactive oxygen species. Further they are also known to increase SGPT activity in humans. Due to the toxicity of pyrethrins in water bodies, soils, and food products, there is an urgent need to develop sustainable approaches to reduce their levels in the respective fields, which are eco-friendly, economically viable, and socially acceptable for on-site remediation. Keeping this in view, an attempt has been made to analyse the advances and prospects in using pyrethrins and possible technologies to control their harmful effects. The pyrethroid types, composition and biochemistry of necessary pyrethroid insecticides have been discussed in detail, in the research paper, along with their effect on insects and humans. It also covers the impact of pyrethroids on different plants and soil microbial flora. The second part deals with the microbial degradation of the pyrethroids through different modes, i.e., bioaugmentation and biostimulation. Many microbes such as Acremonium, Aspergillus, Microsphaeropsis, Westerdykella, Pseudomonas, Staphylococcus have been used in the individual form for the degradation of pyrethroids, while some of them such as Bacillus are even used in the form of consortia. © 2022 Elsevier B.V.
Alpinia officinarum
(Elsevier, 2020) Arpan Mukherjee; Gowardhan Kumar Chouhan; Saurabh Singh; Koustav Chatterjee; Akhilesh Kumar; Anand Kumar Gaurav; Durgesh Kumar Jaiswal; Jay Prakash Verma
Alpinia officinarum Hance (galanga) is a perennial ginger family plant. Galanga has been traditionally used for many years to treat several different diseases including cold, pain, inflammation, stomach ache, and microbial infection, and it also works as an antioxidant and anticancer agent. Different parts of A. officinarum have been used either directly or by processing through different methods for treatment. Plant parts like rhizomes, leaves, aerial parts, and roots of galanga were extracted both in polar and nonpolar solvents to induce the disease control property. Flavonoid extract of this plant is more effective in all kinds of disease. This chapter summarizes the application of A. officinarum in curing different diseases from microbial infection to neural disease. Our main focus in this chapter is on the mechanism of galanga on curing Alzheimer disease. © 2021 Elsevier Inc. All rights reserved.
Bio-fortification of minerals in crops: current scenario and future prospects for sustainable agriculture and human health
(Springer Science and Business Media B.V., 2022) Durgesh Kumar Jaiswal; Ram Krishna; Gowardhan Kumar Chouhan; Arthur Prudêncio de Araujo Pereira; Avinash Bapurao Ade; Satya Prakash; Sunil Kumar Verma; Ram Prasad; Janardan Yadav; Jay Prakash Verma
Minerals are the key factor determining human beings’ optimum growth and development. The deficiencies of minerals and vitamins hinder the human normal growth and development and economic status. In the 21st century, macro and micronutrient deficiencies are significant challenges to improving the nutritional value of foods at the socio-economic level. Bio-fortification is a simple strategy to improve the nutritional value of the human diet. Several bio-fortification strategies, including traditional breeding, transgenic, agronomic, and modernized agriculture practices, were employed to biofortify crops to meet nutritional needs. However, these strategies are also lacking sustainability due to the specific crop species and micronutrients, provision of long-term monitoring and assistance, long term high cost of special chemical fertilizers, losses of crop yield due to chemical fertilizers-induced alterations of plant metabolism, environmental and health impact originating from incorporation new minerals elements like Se and Cu. Therefore, microbial bio-fortification can promote human health and agriculture sustainability. This review highlights; the phyto-availability of micronutrients (Fe, Zn, Mg, Ca, Se, I, & Cu) for human diets; availability of micronutrients value in a stable in the edible part of plants; plant uptakes of micronutrients from the soil system; & strategies of crops fortification and its importance. At present, microbial bio-fortification should be emphasized by exploring the macro and micronutrients regulatory mechanisms through plant-microbe interaction with specific soil systems and climate change. © 2022, The Author(s), under exclusive licence to Springer Nature B.V.
Biogenic factors explain soil carbon in paired urban and natural ecosystems worldwide
(Nature Research, 2023) Manuel Delgado-Baquerizo; Pablo García-Palacios; Mark A. Bradford; David J. Eldridge; Miguel Berdugo; Tadeo Sáez-Sandino; Yu-Rong Liu; Fernando Alfaro; Sebastian Abades; Adebola R. Bamigboye; Felipe Bastida; José L. Blanco-Pastor; Jorge Duran; Juan J. Gaitan; Javier G. Illán; Tine Grebenc; Thulani P. Makhalanyane; Durgesh Kumar Jaiswal; Tina U. Nahberger; Gabriel F. Peñaloza-Bojacá; Ana Rey; Alexandra Rodríguez; Christina Siebe; Alberto L. Teixido; Wei Sun; Pankaj Trivedi; Jay Prakash Verma; Ling Wang; Jianyong Wang; Tianxue Yang; Eli Zaady; Xiaobing Zhou; Xin-Quan Zhou; César Plaza
Urban greenspaces support multiple nature-based services, many of which depend on the amount of soil carbon (C). Yet, the environmental drivers of soil C and its sensitivity to warming are still poorly understood globally. Here we use soil samples from 56 paired urban greenspaces and natural ecosystems worldwide and combine soil C concentration and size fractionation measures with metagenomics and warming incubations. We show that surface soils in urban and natural ecosystems sustain similar C concentrations that follow comparable negative relationships with temperature. Plant productivity’s contribution to explaining soil C was higher in natural ecosystems, while in urban ecosystems, the soil microbial biomass had the greatest explanatory power. Moreover, the soil microbiome supported a faster C mineralization rate with experimental warming in urban greenspaces compared with natural ecosystems. Consequently, urban management strategies should consider the soil microbiome to maintain soil C and related ecosystem services. © 2023, The Author(s), under exclusive licence to Springer Nature Limited.
Biotechnological Interventions in Tomato (Solanum lycopersicum) for Drought Stress Tolerance: Achievements and Future Prospects
(MDPI, 2022) Ram Krishna; Waquar Akhter Ansari; P.S. Soumia; Akhilesh Yadav; Durgesh Kumar Jaiswal; Sudhir Kumar; Achuit Kumar Singh; Major Singh; Jay Prakash Verma
Tomato production is severely affected by abiotic stresses (drought, flood, heat, and salt) and causes approximately 70% loss in yield depending on severity and duration of the stress. Drought is the most destructive abiotic stress and tomato is very sensitive to the drought stress, as cultivated tomato lack novel gene(s) for drought stress tolerance. Only 20% of agricultural land worldwide is irrigated, and only 14.51% of that is well-irrigated, while the rest is rain fed. This scenario makes drought very frequent, which restricts the genetically predetermined yield. Primarily, drought disturbs tomato plant physiology by altering plant–water relation and reactive oxygen species (ROS) generation. Many wild tomato species have drought tolerance gene(s); however, their exploitation is very difficult because of high genetic distance and pre- and post-transcriptional barriers for embryo development. To overcome these issues, biotechnological methods, including transgenic technology and CRISPR-Cas, are used to enhance drought tolerance in tomato. Transgenic technology permitted the exploitation of non-host gene/s. On the other hand, CRISPR-Cas9 technology facilitated the editing of host tomato gene(s) for drought stress tolerance. The present review provides updated information on biotechnological intervention in tomato for drought stress management and sustainable agriculture. © 2022 by the authors.
Characterization and screening of thermophilic Bacillus strains for developing plant growth promoting consortium from hot spring of Leh and Ladakh region of India
(Frontiers Media S.A., 2018) Jay Prakash Verma; Durgesh Kumar Jaiswal; Ram Krishna; Satya Prakash; Janardan Yadav; Vijai Singh
In the present investigation, the main aim is to identify and characterize the potential drought tolerant plant growth promoting consortium for agricultural productivity. Three bacterial isolates were isolated from hot spring of Chumathang area of Leh district. Bacillus species (BHUJP-H1, BHUJP-H2, and BHUJP-H3) were done some biochemical tests including catalase, cellulase, amylase, indole-3-acetic acid, phosphate solubilisation, production of ammonia, siderophore, and hydrogen cyanide. Molecular characterization of isolates was done by 16S rDNA sequencing, e.g., Bacillus subtilis BHUJP-H1 (KU312403), Bacillus sp. BHUJP-H2 (KU312404) and B. licheniformis BHUJP-H3 (KU312405). The genetic diversity of the isolates was assessed by seven inter simple sequence repeat, all primer shows high polymorphism. The highest polymorphism efficiency and polymorphism information content showed by UBC-809 and UBC-836 which were 100% and 0.44 respectively, the lowest is by UBC-807 75% and 0.28 respectively. On an average 90.69% polymorphism efficiency and 0.40 polymorphism information contents obtained by used markers. The highest, 11.08 and the lowest, 4.50 effective multiplex ratios obtained for primer UBC-823 and UBC-807, on an average 7.99 effective multiplex ratio obtained. The highest, 4.89 and the lowest, 1.25 marker indexes obtained by UBC-836 and UBC-807 respectively and on an average 3.24 obtained. The UPGMA cluster analysis divided a population into two clusters I and II, in which BHUJP-H1 and BHUJP-H2 grouped under same while BHUJP-H3 grouped under another cluster. The treatment combination of Bacillus subtilis BHUJP-H1, B. subtilis BHUJP-H1+ B. licheniformis BHUJP-H3 and B. subtilis BHUJP-H1+ Bacillus sp. BHUJP-H2+ B. licheniformis BHUJP-H3 were recorded better combination for enhancing plant growth attributes of Vigna radiata as compared to control and others. The plant growth promoting consortium, e.g., Bacillus subtilis BHUJP-H1, Bacillus subtilis BHUJP-H1+ B. licheniformis BHUJP-H3 and B. subtilis BHUJP-H1+ Bacillus sp. BHUJP-H2+ B. licheniformis BHUJP-H3 can be further used as effective microbial inoculant for enhancing the production of mungbean in field conditions. Bacillus sp. BHUJP-H1 and Bacillus sp. BHUJP-H2 may use as drought tolerant plant growth promoting consortium for enhancing the sustainable agricultural productivity. © 2018 Verma, Jaiswal, Krishna, Prakash, Yadav and Singh.
Climate change: Impact on agricultural production and sustainable mitigation
(Elsevier, 2021) Poonam Yadav; Durgesh Kumar Jaiswal; Rakesh Kumar Sinha
The agricultural is an important sector and shares a major fraction of any nations’ gross national product and, thus, has a key role in the development. Due to the green revolution, crop production has increased to a greater extent in the past decades and simulated to be continued in the coming years. But, at present, one of the challenging issues is food security and maintaining the equitable standard of living for the coming generations. In this regard, one of the major global environmental issues in front of us is human-induced climate change, which threatens our ability to meet the demand. In the past decades, the high growth rate of industrialization, urbanization, and anthropogenic activities has resulted in adverse climate changes, significantly affecting the global temperature, increased greenhouse gases, seasonal variation, and irregular precipitation patterns. According to the Intergovernmental Panel on Climate Change (IPCC) global warming prediction report, the global mean temperature will be increased by 1.0-3.5°C by 2100, and CO2 doubling would be increased temperature by 1.5-4.5°C. Global mean surface temperature anomalies were also recorded to be increasing consistently with time since 1880 to the year 2019; 2016 ranks as the warmest on record (NASA GISS). Thus, increase in temperature and CO2 can be a big challenge in front of farmers and result in a major threat to agriculture and food security. It has the potential to affect agriculture positively as well as negatively in terms of yield, depending upon the variations in various factors. The share of agriculture in global emissions, the need for further global mitigation efforts, and continued projected agricultural emissions growth in many countries all combined together to underline the necessity for stronger and more effective policies. Thus, this chapter is focused on the impact of climate change on the agricultural sector and strategies for mitigating climate change in the agriculture system. In this context, there is an urgent need to analyze the impact of climate change on agriculture productivity and project sustainable approaches to adapt the climate change and meet the future food demand. © 2021 Elsevier Inc. All rights reserved.
Co-overexpression of AtDREB1A and BcZAT12 increases drought tolerance and fruit production in double transgenic tomato (Solanum lycopersicum) plants
(Elsevier B.V., 2021) Ram Krishna; Waquar Akhter Ansari; Durgesh Kumar Jaiswal; Achuit Kumar Singh; Jay Prakash Verma; Major Singh
Drought is the major problem in agricultural production due to loss of moisture content in soil as well as climate variations. Our main aim is to enhance drought tolerance and yield potential in the present study pyramided Arabidopsis thaliana Dehydration Responsive Element Binding1A (AtDREB1A) and Brasica caranata Zinc finger proteins (BcZAT12) transcription factor genes driven by ectopic promoter rd29 A of Arabidopsis thaliana and Brassica carinata lea1, respectively. Co-overexpression of both the genes provides tolerance to multiple abiotic stresses but the AtDREB1A overexpression has been reported to cause retarded growth and dwarf phenotype; however BcZAT12 overexpressing transgenic plants does not show retarded growth and dwarf phenotype. Co-overexpressing of AtDREB1A and BcZAT12 in five (DZ1-DZ5) double transgenic (DT) tomato lines has been observed under 0, 07, 14 and 21 Days of Water Deficit (DWD). The DT plants showed enhanced drought tolerance and yield potential than single transgenic (ST) and non transgenic (NT) plants. Furthermore, AtDREB1A and BcZAT12 co-overexpressed plants showed reduced level of electrolyte leakage (EL), hydrogen peroxide and membrane lipid peroxidation and elevated level of relative water content (RWC), proline, chlorophyll color index (CCI) and photosynthetic efficiency as compared to ST and NT. The plant growth and yield attributes were improved by the co-overexpression of AtDREB1A and BcZAT12 in DT plants. The transcript analysis showed the increased level of DREB1A, ZAT12 and P5CS genes expression which were higher in DT tomato plants, and indicate that both the genes induce together in the DT plants. The present study which is first report of co-overexpressing AtDREB1A and BcZAT12 in tomato will provide a base for genetic engineering in plants through the multigenic transgenic approach to cope against various biotic and abiotic stresses. © 2021 Elsevier B.V.
Developing efficient thermophilic cellulose degrading consortium for glucose production from different agro-residues
(Frontiers Media S.A., 2019) Saurabh Singh; Durgesh Kumar Jaiswal; Nallusamy Sivakumar; Jay Prakash Verma
In the present study, 11 cellulose degrading bacterial strains were isolated from water and soil samples of hot springs in the Chumathang village, Leh and Ladakh region, India. The isolated strains were identified as Bacillus subtilis, Bacillus aryabhattai, Bacillus stratosphericus, Bacillus altitudinis, and Brevibacterium frigoritolerans by biochemical and molecular approaches. All the strains were evaluated for the total cellulase, endoglucanase, exoglucanase, and β-glucosidase enzyme activities. On the basis of overall individual cellulose degrading enzyme activities, three strains were selected to develop consortium to enhance their cellulase enzyme activities. The potent cellulose degraders B. stratosphericus BHUJPV-H5, B. subtilis BHUJPV-H19, and B. subtilis BHUJPV-H12 were selected for the consortium development. The effect of cellulase activities of bacterial strains were evaluated ranged up to 6.06 and 0.72mg ml-1 glucose by agro-residues of sugarcane bagasse and wheat straw, respectively, after 1 h of incubation. Total cellulase enzyme activity of consortia was recorded two times higher than the individual organisms. These strains can be used for enhancing bioethanol production from lignocellulosic biomass, which can consequently boost biofuel production industry. © 2019 Singh, Jaiswal, Sivakumar and Verma.
Development of indigenous microbial consortium for biocontrol management
(Elsevier, 2020) Arpan Mukherjee; Gowardhan Kumar Chouhan; Anand Kumar Gaurav; Durgesh Kumar Jaiswal; Jay Prakash Verma
Excessive use of chemicals as fertilizer in agriculture to induce crop production in recent year is a potent agent of environment pollution and a serious threat to health. A lot of alternative sources (biofertilizers, biopesticides, INM, IPM, PGPR/PGPM, vermicompost, green manuring, and crop rotation) are now available to minimize the use of chemical fertilizer and pesticides. Different soil microbes have potential ability to improve the productivity and nutritional quality as well as soil fertility and health. To date, many reports are available on single or dual consortium of microbes used as biofertilizers and biopesticides for enhancing crop production. Most recently, phytomicrobiome studies are offering further insights into alternative use of biological intervention for enhancing agricultural production. Under this approaches the whole microbiome community is considered for multiple application for survival of plant under both normal and stress condition. That may be culturable and nonculturable microbes. Culturable microbes are able to enhance the plant growth via either direct or indirect mechanism including biological nitrogen fixation, solubilization of inorganic nutrient, production of phytohormones, siderophore, HCN, chitinase, and antibiotics and help to induce systemic resistance (ISR) and systemic acquired resistance (SAR) against a wide group of plant pathogen to protect the plant. If microbial isolate exhibiting different properties are mixed together, it is possible that they enhance each other’s property. The use of such consortia in field inoculation has great potential to facilitate crop protection and enhance yield. Because single microbes can’t play different roles that help the crops for their growth and protection too, so we need a potential consortium for better yield and growth. Therefore the main aim of this chapter is to review and highlight the impact of microbial consortium for biocontrol management of plants. © 2021 Elsevier B.V. All rights reserved.
Editorial: Microbial co-cultures: a new era of synthetic biology and metabolic engineering
(Frontiers Media SA, 2023) Durgesh Kumar Jaiswal; Jay Prakash Verma; Tarun Belwal; Arthur Prudêncio De Araujo Pereira; Avinash Bapurao Ade
[No abstract available]
Editorial: Microbial co-cultures: a new era of synthetic biology and metabolic engineering, volume II
(Frontiers Media SA, 2025) Durgesh Kumar Jaiswal; Avinash Bapurao Ade; Tarun Belwal; Arthur Prudêncio de Araujo Pereira; Jay Prakash Prakash Verma
[No abstract available]
Editorial: Sustainability in integrated food systems for biovalorization and circular bioeconomy
(Frontiers Media SA, 2025) Saurabh Singh; Durgesh Kumar Jaiswal; Raj Morya; Jay Prakash Prakash Verma; Rajesh Kumar Sani
[No abstract available]
Effects of Salt Stress on Biochemistry of Crop Plants
(wiley, 2021) Poonam Yadav; Durgesh Kumar Jaiswal
Sodic soil is thought to be dynamic and spreading globally to influence the world all the more extensively and broadly. Impact on coastal areas due to sea-level rise, and the temperature rise that will result in increased evaporation and further soil salinization in the coming years. There are more than 100 countries all over the world under the influence of salinity and no land is completely free from salinity. Soil salinization is one of the primary explanations behind soil well-being and microbiome system disintegration in degraded arid and semiarid regions of the world; hence, soil salinity is the most serious factor that influences the development of plants. Germination and developmental process may be affected under the sodic soil condition. Salts influence plants in many ways by making water less accessible, and high saltiness is viewed as a physiological dry season. Depending upon the plants and abiotic/biotic stresses, an imbalance in reactive oxygen species and reactive nitrogen species metabolisms can occur. This leads to nitro-oxidative stress in plants' cell, with antioxidant systems (enzymatic and nonenzymatic), plants' redox status (NADPH, NADH, GSH/GSSG), and other responsive-defensive systems (membrane lipid and protein biosynthesis, mRNA, hormones and ion signaling) being affected differently. In this review, plants’ biochemical mechanisms and major stress-responsive genes under salt stress and interaction with DNA-binding proteins that are controlling these genes and related mechanisms under the salt stress are discussed. Therefore, the biochemical mechanisms of salinity tolerance need to be defined to provide sufficient indicators for plant breeders. © 2022 John Wiley & Sons Ltd. All rights reserved.
Evaluation of PGPR and different concentration of phosphorus level on plant growth, yield and nutrient content of rice (Oryza sativa)
(Elsevier B.V., 2014) Lavakush; Janardan Yadav; Jay Prakash Verma; Durgesh Kumar Jaiswal; Ashok Kumar
The aims of this study were to boost growth attributes, yield and nutrient uptake of rice by different treatment combination of plant growth promoting rhizobacteria (PGPR) and various phosphorus fertilizer doses. PGPR strains e.g. Pseudomonas aeruginosa BHUJY16, P. aeruginosa BHUJY20, Pseudomonas putida BHUJY13, P. putida BHUJY23 and Pseudomonas fluorescens BHUJY29 were known as combined Pseudomonas culture (CPC). The treatment combinations of (CPC+Azotobacter chroococcum+Azospirillum brasilense+60kgha-1 P2O5) and (CPC+A. chroococcum+A. brasilense+30kgha-1 P2O5) showed greater significant (31.66 and 32.00gpot-1) and (29.99 and 30.28gpot-1) grain yield of rice as compared to control during first and second year pot experiments, respectively. The treatment combination (CPC+A. chroococcum+A. brasilense+60kgha-1 P2O5) was recorded significantly higher plant growth attribute, yield, nutrient contents in grain and straw of rice followed by combination of (CPC+A. chroococcum+A. brasilense+30kgha-1 P2O5), (CPC+A. chroococcum+60kgha-1 P2O5) as compared to control during both year of experiments. PGPR combination with 60kgha-1 phosphorus (P) was found higher growth and yield; while PGPR with 30kgha-1 P gave approximately similar results of growth and yield due to more availability of NPK in the soil by cumulative effect of PGPR activities in the rhizosphere soil. So that treatment combinations of PGPR with 30kgha-1 P was found economically cheaper than the PGPR with 60kgha-1 P. Hence, the treatment combination of PGPR strains of Pseudomonas culture with A. chroococcum+A. brasilense+30kgha-1 P2O5 may be used as more effective combination for rice production. © 2013 Elsevier B.V.
Evaluation of plant growth promoting activities of microbial strains and their effect on growth and yield of chickpea (Cicer arietinum L.) in India
(2014) Jay Prakash Verma; Janardan Yadav; Kavindra Nath Tiwari; Durgesh Kumar Jaiswal
The aims of our study were to enhance growth, yield and disease control of chickpea by various combinations of microbial strains (Mesorhizobium, Azotobacter chroococcum, Pseudomonas aeruginosa and Trichoderma harzianum). Pseudomonas and Trichoderma showed positive IAA (indole-3-acetic acid) production, phosphate solubilisation and antagonistic activities against Fusarium oxysporum and Rhizoctonia solani as compared to other strains. In two year investigations, tetra-inoculants have shown significant growth attributes, yield and phytopathogen growth inhibition followed by tri-inoculants than control. Therefore, tetra-inoculants (Mesorhizobium- Azotobacter-Pseudomonas-Trichoderma) may be used as efficient biofertilizer and bio-control agent for chickpea production (Cicer arietinum L.) in eastern Uttar Pradesh. © 2013 Elsevier Ltd.
Functional metagenomics: A potential tool for mining of biomolecules from environmental samples
(Elsevier Inc., 2025) Vivek Kumar; Anjali Singh; Vijay Shankar Singh; Rajan Chaurasia; Mahesh Rao; Pramod Kumar Sahu; Durgesh Kumar Jaiswal
Functional metagenomics is emerging as a transformative tool for discovering biomolecules from environmental samples, offering unprecedented insights into microbial diversity and functionality. This review explores the potential of functional metagenomics to uncover novel enzymes, antibiotics, and therapeutic compounds by analyzing environmental DNA (eDNA) without the constraints of traditional culture-based methods. The vast microbial biomass in diverse ecosystems, from soil to extreme habitats, harbors a wealth of genetic resources that can be harnessed for biotechnological applications. Researchers can use modern sequencing techniques and functional screening approaches to find and characterize genes with advantageous features, such as bioremediation capacities and industrially relevant enzymes. Despite challenges in DNA extraction and host expression systems, integrating functional metagenomics with multi-omics approaches promises to enhance our understanding of microbial interactions and facilitate the development of eco-friendly bioprocesses. This review underscores the importance of functional metagenomics in expanding our repertoire of biomolecules and highlights its potential to significantly impact biotechnology and environmental sustainability. © 2025 Elsevier Inc.
Harnessing bacterial strain from rhizosphere to develop indigenous PGPR consortium for enhancing lobia (Vigna unguiculata) production
(Elsevier Ltd, 2023) Jay Prakash Verma; Durgesh Kumar Jaiswal; Anand Kumar Gaurav; Arpan Mukherjee; Ram Krishna; Arthur Prudêncio de Araujo Pereira
The rhizosphere microbes play a key role in plant nutrition and health. However, the interaction of beneficial microbes and Vigna unguiculata (lobia) production remains poorly understood. Thus, we aimed to isolate and characterize the soil microbes from the rhizosphere and develop novel microbial consortia for enhancing lobia production. Fifty bacterial strains were isolated from the rhizosphere soil samples of lobia. Finally, five effective strains (e.g., Pseudomonas sp. IESDJP-V1 and Pseudomonas sp. IESDJP-V2, Serratia marcescens IESDJP-V3, Bacillus cereus IESDJP-V4, Ochrobactrum sp. IESDJP-V5) were identified and molecularly characterized by 16 S rDNA gene amplification. All selected strains showed positive plant growth promoting (PGP) properties in broth culture. Based on morphological, biochemical, and plant growth promoting activities, five effective isolated strains and two collected strains (Azospirillum brasilense MTCC-4037 and Paenibacillus polymyxa BHUPSB17) were selected. The pot trials were conducted with seed inoculations of lobia (Vigna unguiculata) var. Kashi Kanchan with thirty treatments and three replications. The treatment combination T3 (Pseudomonas sp. IESDJP-V2), T14 (Pseudomonas sp. IESDJP-V2 + A. brasilense), T26 (Pseudomonas sp. IESDJP-V1+ B. cereus IESDJP-V4 + P. polymyxa) and T27 (IESDJP-V1+ IESDJP-V5+ A. brasilense) were recorded for enhancing plant growth attributes, yield, nutritional content like protein, total sugar, flavonoid and soil properties as compared to control and others. The effective treatments T3 (Pseudomonas sp.), T14 (Pseudomonas sp. IESDJP-V2 + A. brasilense), T26 (Pseudomonas sp. IESDJP-V1+ B. cereus IESDJP-V4 + P. polymyxa) and T27 (IESDJP-V1+ IESDJP-V5+ A. brasilense) recorded as potential PGPR consortium for lobia production. The treatment of single (Pseudomonas sp.), duel (IESDJP-V2 + A. brasilense) and triple combination (IESDJP-V1+ IESDJP-V4 + P. polymyxa) and (IESDJP-V1+ IESDJP-V5+ A. brasilense) can be further used for developing effective indigenous consortium for lobia production under sustainable farming practices. These PGPR bio-inoculant will be cost-effective, environment-friendly and socially acceptable. © 2023 The Authors
Harnessing of phytomicrobiome for developing potential biostimulant consortium for enhancing the productivity of chickpea and soil health under sustainable agriculture
(Elsevier B.V., 2022) Arpan Mukherjee; Saurabh Singh; Anand Kumar Gaurav; Gowardhan Kumar Chouhan; Durgesh Kumar Jaiswal; Arthur Prudêncio de Araujo Pereira; Ajit Kumar Passari; Ahmed M. Abdel-Azeem; Jay Prakash Verma
The main aim of the present work was to explore culturable bacteria and to develop potential microbial consortium as bio-inoculants for enhancing plant productivity, nutritional content, and soil health. For this study, we selected two bacterial strains e.g., Enterobacter hormaechei (BHUJPCS-15) and Brevundimonas naejangsanensis (BHUJPVCRS-1) based on plant growth-promoting activities We developed a consortium of both strains and estimated plant growth promotion (PGP) activity which recorded significant better production of Indole-3-acetic acid (IAA) (61.53 μg/ml), siderophore (12.66%), ammonia (98.66 μg/ml), phosphate solubilisation (942.64 μg/ml), potassium solubilisation, and antagonistic activity against Fusarium sp. than individual bacterial strains. Bacterial consortium (E. hormaechei + B. naejangsanensis) treatment significantly enhanced plant growth attributes, grain yields, nutritional content in plant and seed, followed by E. hormaechei as compared to control. Seed treated with consortium recorded a significant increase in available N P K, enzymes and microbial communities in soils. Microbiome analysis revealed that the dominance of bacterial group and its functional properties is directly correlated with plant growth attributes, nutrient content, soil N P K, and enzyme activity. The relative abundance of bacterial phyla Proteobacteria (98%) was dominantly recorded in all treatments. The microbiome of seed and soil, treated with consortium (E. hormaechei + B. naejangsanensis) showed high amount of diversity of bacterial phyla Verrucomicrobia, Firmicutes, Bacteroidetes, Acidobacteria, Chloroflexi, and Proteobacteria than E. hormaechei (Firmicutes, Bacteroidetes, Chloroflexi and Proteobacteria) and control (Firmicutes, Bacteroidetes and Proteobacteria). In soil, root and shoot, E. hormaechei treatment enriched ligninolytic, nitrogen fixation, cellulolytic, nitrate ammonification among other pathways. The main finding is that the consortium treated seed of chickpea recorded significant enhancement of plant growth attributes, productivity, nutritional content, and soil health as well as microbial colonization in soil and seed part. © 2022 Elsevier B.V.
Impact of Plant Growth-Promoting Microorganism (PGPM) Consortium on Biochemical Properties and Yields of Tomato Under Drought Stress
(Multidisciplinary Digital Publishing Institute (MDPI), 2024) Ram Krishna; Waquar Akhter Ansari; Mohammad Altaf; Durgesh Kumar Jaiswal; Sudhakar Pandey; Achuit Kumar Singh; Sudhir Kumar; Jay Prakash Verma
Drought is the most important abiotic stress that restricts the genetically predetermined yield potential of the crops. In the present study, four tomato varieties: Kashi Vishesh, Kashi Aman, Kashi Abhiman, and Kashi Amrit, were used to study the effect of PGPMs (plant growth-promoting microorganisms). PGPM strains, Bacillus megaterium BHUPSB14, Pseudomonas fluorescens BHUPSB06, Pseudomonas aeruginosa BHUPSB01, Pseudomonas putida BHUPSB0, Paenibacillus polymixa BHUPSB17, and Trichoderma horzianum, were used as the consortium. The control group was irrigated up to 80% of field capacity, while 7-, 14-, and 21-day water-deficit-exposed (DWD) plants’ pot soil moisture was maintained to 40, 25, and 15% of the field capacity, both with and without the PGPM inoculation condition. The physiological parameters, such as electrolyte leakage, relative water content, photosynthetic efficiency, and chlorophyll color index, were significantly improved by PGPM application under progressive drought stress, compared to the control. PGPM application enhanced the proline accumulation and reduced the formation of hydrogen peroxide and lipid peroxidation under drought stress. The plant growth attributes were significantly increased by PGPM application. The Kashi Amrit variety showed the highest fruit yield among the four varieties under all the treatments. The PGPM consortium application also improved the soil physico-biological properties and nutrient availability in the soil. The PGPM consortium used in this study can potentially mitigate drought stress on tomato in drought-prone regions and act as a biofertilizer. The present study will open a new avenue of drought stress management in tomato. © 2024 by the authors.