Browsing by Author "Estibaliz Sansinenea"

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Antimicrobial secondary metabolites from agriculturally important bacteria as next-generation pesticides
(Springer, 2020) Chetan Keswani; Harikesh B. Singh; Carlos García-Estrada; John Caradus; Ya-Wen He; Samia Mezaache-Aichour; Travis R. Glare; Rainer Borriss; Estibaliz Sansinenea
The whole organisms can be packaged as biopesticides, but secondary metabolites secreted by microorganisms can also have a wide range of biological activities that either protect the plant against pests and pathogens or act as plant growth promotors which can be beneficial for the agricultural crops. In this review, we have compiled information about the most important secondary metabolites of three important bacterial genera currently used in agriculture pest and disease management. © 2019, Springer-Verlag GmbH Germany, part of Springer Nature.
Antimicrobial secondary metabolites from agriculturally important fungi as next biocontrol agents
(Springer, 2019) Chetan Keswani; Harikesh B. Singh; Rosa Hermosa; Carlos García-Estrada; John Caradus; Ya-Wen He; Samia Mezaache-Aichour; Travis R. Glare; Rainer Borriss; Francesco Vinale; Estibaliz Sansinenea
Synthetic chemical pesticides have been used for many years to increase the yield of agricultural crops. However, in the future, this approach is likely to be limited due to negative impacts on human health and the environment. Therefore, studies of the secondary metabolites produced by agriculturally important microorganisms have an important role in improving the quality of the crops entering the human food chain. In this review, we have compiled information about the most important secondary metabolites of fungal species currently used in agriculture pest and disease management. © 2019, Springer-Verlag GmbH Germany, part of Springer Nature.
Auxins of microbial origin and their use in agriculture
(Springer Science and Business Media Deutschland GmbH, 2020) Chetan Keswani; Satyendra Pratap Singh; Laura Cueto; Carlos García-Estrada; Samia Mezaache-Aichour; Travis R. Glare; Rainer Borriss; Surya Pratap Singh; Miguel Angel Blázquez; Estibaliz Sansinenea
To maintain the world population demand, a sustainable agriculture is needed. Since current global vision is more friendly with the environment, eco-friendly alternatives are desirable. In this sense, plant growth–promoting rhizobacteria could be the choice for the management of soil-borne diseases of crop plants. These rhizobacteria secrete chemical compounds which act as phytohormones. Indole-3-acetic acid (IAA) is the most common plant hormone of the auxin class which regulates various processes of plant growth. IAA compound, in which structure can be found a carboxylic acid attached through a methylene group to the C-3 position of an indole ring, is produced both by plants and microorganisms. Plant growth–promoting rhizobacteria and fungi secrete IAA to promote the plant growth. In this review, IAA production and mechanisms of action by bacteria and fungi along with the metabolic pathways evolved in the IAA secretion and commercial prospects are revised. Key points • Many microorganisms produce auxins which help the plant growth promotion. • These auxins improve the plant growth by several mechanisms. • The auxins are produced through different mechanisms. © 2020, Springer-Verlag GmbH Germany, part of Springer Nature.
Bacillus spp. as Bio-factories for Antifungal Secondary Metabolites: Innovation Beyond Whole Organism Formulations
(Springer, 2023) Bruno Salazar; Aurelio Ortiz; Chetan Keswani; Tatiana Minkina; Saglara Mandzhieva; Satyendra Pratap Singh; Bhagwan Rekadwad; Rainer Borriss; Akansha Jain; Harikesh B. Singh; Estibaliz Sansinenea
Several fungi act as parasites for crops causing huge annual crop losses at both pre- and post-harvest stages. For years, chemical fungicides were the solution; however, their wide use has caused environmental contamination and human health problems. For this reason, the use of biofungicides has been in practice as a green solution against fungal phytopathogens. In the context of a more sustainable agriculture, microbial biofungicides have the largest share among the commercial biocontrol products that are available in the market. Precisely, the genus Bacillus has been largely studied for the management of plant pathogenic fungi because they offer a chemically diverse arsenal of antifungal secondary metabolites, which have spawned a heightened industrial engrossment of it as a biopesticide. In this sense, it is indispensable to know the wide arsenal that Bacillus genus has to apply these products for sustainable agriculture. Having this idea in our minds, in this review, secondary metabolites from Bacillus having antifungal activity are chemically and structurally described giving details of their action against several phytopathogens. Knowing the current status of Bacillus secreted antifungals is the base for the goal to apply these in agriculture and it is addressed in depth in the second part of this review. © 2022, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.
Bioengineering Bacillus spp. for Sustainable Crop Production: Recent Advances and Resources for Biotechnological Applications
(Springer, 2025) Aurelio Ortiz; Estibaliz Sansinenea; Keswani Chetan; Tatiana Mikhailovna Minkina; Satyendra Pratap Singh; Bhagwan Narayan Rekadwad; Rainer Borriss; Kathleen Laura Hefferon; Trinh Xuan Hoat; Debasis Mitra; Pradeep Kumar Das Mohapatra; Periyasamy Panneerselvam
The goal of sustainable agriculture is to meet the rising need for food, while minimizing adverse impacts on the environment, protecting natural resources, and ensuring agricultural output over the long term. The pressing need to increase agricultural yield through sustainable agriculture is being emphasized. Several Bacillus species have been used as commercial biopesticides since they can act against plant pathogens by potentially suppressing them. At the same time, they can act as plant growth-promoting rhizobacteria and are known for their diverse characteristics and beneficial properties, making them potential candidates for use sustainable crop production programs. Knowledge of genetic information opens the door of possibility for understanding the way these microorganisms behave. By applying biotechnological tools to Bacillus, strategies can be adopted for the purpose of increasing the yield of crops and managing pests and pathogens that infect them. In this review, we identify the genes in the most significant Bacillus spp. that contribute to plant improvement. The most important biotechnological tools and advance computational approaches are described to provide an extended vision on this topic. However, increasing the crop production through application of beneficial microbial strains requires a multifaceted approach that considers ecological, economic, and social aspects. By implementing these strategies and practices, we can work towards a sustainable and resilient agricultural system that meets the growing food demand, while preserving the environment for future generations. © The Author(s) 2024.
Bioengineering Bacillus spp. for Sustainable Crop Production: Recent Advances and Resources for Biotechnological Applications
(Springer, 2024) Aurelio Ortiz; Estibaliz Sansinenea; Chetan Keswani; Tatiana Minkina; Satyendra Pratap Singh; Bhagwan Rekadwad; Rainer Borriss; Kathleen Hefferon; Trinh Xuan Hoat; Debasis Mitra; Pradeep Kumar Das Mohapatra; Periyasamy Panneerselvam
The goal of sustainable agriculture is to meet the rising need for food, while minimizing adverse impacts on the environment, protecting natural resources, and ensuring agricultural output over the long term. The pressing need to increase agricultural yield through sustainable agriculture is being emphasized. Several Bacillus species have been used as commercial biopesticides since they can act against plant pathogens by potentially suppressing them. At the same time, they can act as plant growth-promoting rhizobacteria and are known for their diverse characteristics and beneficial properties, making them potential candidates for use sustainable crop production programs. Knowledge of genetic information opens the door of possibility for understanding the way these microorganisms behave. By applying biotechnological tools to Bacillus, strategies can be adopted for the purpose of increasing the yield of crops and managing pests and pathogens that infect them. In this review, we identify the genes in the most significant Bacillus spp. that contribute to plant improvement. The most important biotechnological tools and advance computational approaches are described to provide an extended vision on this topic. However, increasing the crop production through application of beneficial microbial strains requires a multifaceted approach that considers ecological, economic, and social aspects. By implementing these strategies and practices, we can work towards a sustainable and resilient agricultural system that meets the growing food demand, while preserving the environment for future generations. © The Author(s) 2024.
Biological synthesis of nanofertilizer and their effects on crop health
(Nova Science Publishers, Inc., 2021) S.P. Singh; Soumya Subhadarshini; Hagera Dilnashin; Chetan Keswani; Estibaliz Sansinenea; Sudhir S. Shende
After green revolution, nanotechnology has been evolved as the sixth most important revolutionary technology. The green methods i.e., biological approaches used in the synthesis of nanoparticles (NPs) and their application are a great hope for sustainable agriculture practices. Nanotechnology has provided tremendous application by virtue of nanoparticles in agriculture sector which includes nanosensor, nanopesticide, nanofertilizer and smart delivery system for regulating the release of agrochemicals. In the present chapter, the recent advances in the field of biologically synthesised NPs for the development of nanofertilizers, and their effect on the agricultural crops are comprehensively reviewed along with the mechanisms of synthesis of NPs. © 2021 Nova Science Publishers, Inc.
Biosynthesis and beneficial effects of microbial gibberellins on crops for sustainable agriculture
(Oxford University Press, 2022) Chetan Keswani; Satyendra P. Singh; Carlos García-Estrada; Samia Mezaache-Aichour; Travis R. Glare; Rainer Borriss; Vishnu D. Rajput; Tatiana M. Minkina; Aurelio Ortiz; Estibaliz Sansinenea
Soil microbes promote plant growth through several mechanisms such as secretion of chemical compounds including plant growth hormones. Among the phytohormones, auxins, ethylene, cytokinins, abscisic acid and gibberellins are the best understood compounds. Gibberellins were first isolated in 1935 from the fungus Gibberella fujikuroi and are synthesized by several soil microbes. The effect of gibberellins on plant growth and development has been studied, as has the biosynthesis pathways, enzymes, genes and their regulation. This review revisits the history of gibberellin research highlighting microbial gibberellins and their effects on plant health with an emphasis on the early discoveries and current advances that can find vital applications in agricultural practices. © 2021 The Society for Applied Microbiology.
Correlation of the Effect of Native Bioagents on Soil Properties and Their Influence on Stem Rot Disease of Rice
(Multidisciplinary Digital Publishing Institute (MDPI), 2023) Sowmya Vanama; Maruthi Pesari; Gobinath Rajendran; Uma Devi Gali; Santosha Rathod; Rajanikanth Panuganti; Srivalli Chilukuri; Kannan Chinnaswami; Sumit Kumar; Tatiana Minkina; Estibaliz Sansinenea; Chetan Keswani
Soil is a crucial component for plant growth, as it provides water, nutrients, and mechanical support. Various factors, such as crop cultivation, microflora, nutrient addition, and water availability, significantly affect soil properties. Maintaining soil health is important, and one approach is the introduction of native organisms with multifaceted activities. The study evaluates the effects of introducing these microbes (Trichoderma asperellum strain TAIK1, Bacillus cabrialesii strain BIK3, Pseudomonas putida strain PIK1, and Pseudomonas otitidis strain POPS1) and their consortium, a combination of four bioagents, on soil health, plant growth, and the incidence of stem rot disease caused by Sclerotium oryzae in rice. Upon treatment of soil with the consortium of the four native bioagents mentioned above through seed treatment or soil application, variations/increases in the chemical properties of the soil were observed, viz., pH (8.08 to 8.28), electrical conductivity (EC) (0.72 to 0.75 d S m−1), organic carbon (OC) (0.57 to 0.68 %), available soil nitrogen (SN) (155 to 315 kg/ha), soil phosphorus (SP) (7.87 to 24.91 kg/ha), soil potassium (SK) (121.29 to 249.42 kg/ha), and soil enzymes (urease (0.73 to 7.33 µg urea hydrolyzed g−1 soil h−1), acid and alkaline phosphatase (0.09 to 1.39 and 0.90 to 1.78 µg of p-nitrophenol released g−1 soil h−1), and dehydrogenase (0.14 to 16.44 mg triphenyl formazan (TPF) produced g−1 soil h−1)), compared to untreated soil. Treatment of seeds with the consortium of four native bioagents resulted in a significant increase in plant height (39.16%), the number of panicles (30.29%), and average grain yield (41.36%) over control plants. Under controlled conditions, the bioagent-treated plants showed a 69.37% reduction in stem rot disease. The findings of this study indicate a positive correlation between soil properties (pH, EC, OC, SN, SP, SK, and soil enzymes) and plant growth (shoot and root length, fresh and dry weight) as well as a highly negative association of soil properties with stem rot disease severity. The results suggest that using native bioagents as a management strategy can control stem rot disease and enhance crop productivity, while reducing reliance on chemical management. These findings provide valuable insights into the development of sustainable agricultural practices that maximize productivity by minimizing negative environmental impacts, which promotes soil health, plant growth, and disease management. © 2023 by the authors.
Dual Trichoderma consortium mediated elevation of systemic defense response against early blight in potato
(Springer Science and Business Media B.V., 2022) Sumit Kumar; Ram Chandra; Lopamudra Behera; Chetan Keswani; Estibaliz Sansinenea
The annual crop loss caused due to phytopathogens is a serious problem affecting food security. To overcome the losses due to phytopathogens, the application of toxic pesticides is the only alternative but pose a serious threat to human and environmental health. Hence, more eco-friendly and sustainable approaches for efficient management of phytopathogens are urgently required. In this study, we evaluated the effectiveness of Trichoderma viride and Trichoderma harzianum as single as well as in consortium for elevating the systemic defense response and growth promotion activities in potato challenged with early blight pathogen Alternaria solani. The percent of mycelial inhibition of A. solani by T. viride (91.88%) and T. harzianum (80.11%) was recorded and compared with control. Scanning electron microscope (SEM) observations revealed the collapsed hyphae and sunken conidia of A. solani due to antagonistic activity of T. viride and T. harzianum. Induction of defense enzymes including total phenolic content (TPC), phenylalanine ammonia lyase (PAL), superoxide dismutase (SOD) and total protein content was increased 3.19, 3.72, 1.99 and 2.5 folds, respectively in consortia of Trichoderma spp. treated plants as compared to pathogen infected plants at 48 hapi. HPLC analysis demonstrated higher production free poly-phenolic compounds during combined application of Trichoderma spp. treated potato plants in the response of A. solani infection. This study demonstrates that the consortium of Trichoderma spp. is effective in elevating the host defense response by modulating the activities of phenylpropanoid derivatives, pathogenesis related proteins (PR-proteins), enzymes of oxidative stress network, and growth parameters in potato challenged with the early blight pathogen, A. solani. © 2021, Koninklijke Nederlandse Planteziektenkundige Vereniging.
Nano-Inputs: A Next-Generation Solution for Sustainable Crop Production
(Springer, 2023) Satyendra Pratap Singh; Chetan Keswani; Tatiana Minkina; Aurelio Ortiz; Estibaliz Sansinenea
A sustainable agriculture is a challenge for human health. In the agriculture field, the factors like stress created due to the climate change and the newly emerging pathogens, their recognition, and the intensity of damage that they can do are some of the vital challenges in plant disease management worldwide. There is an urgent call for adoption of environmentally friendly biopesticides/biofertilizers to apply to agriculture. In this context, nanomaterials promise to support this transition by promoting mitigation, enhancing productivity, and reducing contamination. Nanotechnology can be used in agriculture to reduce the use of chemicals, decrease nutrient losses, and increase crops’ yield. The nanotechnology can be applied in the form of nanofertilizers and nanopesticides to increase the crops production. Besides, nanosensors act monitoring soil quality of agricultural field and maintaining the health of agricultural plants. Although large-scale production and in-field testing of nano-agrochemicals are still ongoing, the collected information indicates improvements in uptake, use efficiency, targeted delivery of the active ingredients, and reduction of leaching and pollution. In this review we summarize how nanotechnology impacts in agriculture. © 2023, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.
Preface
(Springer Singapore, 2019) Harikesh Bahadur Singh; Chetan Keswani; M.S. Reddy; Estibaliz Sansinenea; Carlos García-Estrada
[No abstract available]
Re-addressing the biosafety issues of plant growth promoting rhizobacteria
(Elsevier B.V., 2019) Chetan Keswani; Om Prakash; Nidhi Bharti; Juan I. Vílchez; Estibaliz Sansinenea; Richard D. Lally; Rainer Borriss; Surya P. Singh; Vijai K. Gupta; Leonardo F. Fraceto; Renata de Lima; Harikesh B. Singh
To promote agronomic sustainability, extensive research is being carried out globally, investigating biofertilizer development. Recently, it has been realized that some microorganisms used as biofertilizers behave as opportunistic pathogens and belong to the biosafety level 2 (BSL-2) classification. This poses serious risk to the environmental and human health. Evidence presented in various scientific forums is increasingly favoring the merits of using BSL-2 microorganisms as biofertilizers. In this review, we emphasize that partial characterization based on traditional microbiological approaches and small subunit rRNA gene sequences/conserved regions are insufficient for the characterization of biofertilizer strains. It is advised herein, that research and industrial laboratories developing biofertilizers for commercialization or environmental release must characterize microorganisms of interest using a multilateral polyphasic approach of microbial systematics. This will determine their risk group and biosafety characteristics before proceeding with formulation development and environmental application. It has also been suggested that microorganisms belonging to risk-group-1 and BSL-1 category should be used for formulation development and for field scale applications. While, BSL-2 microorganisms should be restricted for research using containment practices compliant with strict regulations. © 2019 Elsevier B.V.
Secondary metabolites of plant growth promoting rhizomicroorganisms: Discovery and applications
(Springer Singapore, 2019) Harikesh Bahadur Singh; Chetan Keswani; M.S. Reddy; Estibaliz Sansinenea; Carlos García-Estrada
Recent changes in the pattern of agricultural practices from use of hazardous pesticides to natural (organic) cultivation has brought into focus the use of agriculturally important microorganisms for carrying out analogous functions. The reputation of plant growth promoting rhizomicroorganisms (PGPRs) is due to their antagonistic mechanisms against most of the fungal and bacterial phytopathogens. The biocontrol potential of agriculturally important microorganisms is mostly attributed to their bioactive secondary metabolites. However, low shelf life of many potential agriculturally important microorganisms impairs their use in agriculture and adoption by farmers. The focal theme of this book is to highlight the potential of employing biosynthesized secondary metabolites (SMs) from agriculturally important microorganisms for management of notorious phytopathogens, as a substitute of the currently available whole organism formulations and also as alternatives to hazardous synthetic pesticides. Accordingly, we have incorporated a comprehensive rundown of sections which particularly examine the SMs synthesized, secreted and induced by various agriculturally important microorganisms and their applications in agriculture. Section 1 includes discussion on biosynthesized antimicrobial secondary metabolites from fungal biocontrol agents. This section will cover the various issues such as development of formulation of secondary metabolites, genomic basis of metabolic diversity, metabolomic profiling of fungal biocontrol agents, novel classes of antimicrobial peptides. The section 1 will also cover the role of these secondary metabolites in antagonist-host interaction and application of biosynthesized antimicrobial secondary metabolites for management of plant diseases. Section 2 will discuss the biosynthesized secondary metabolites from bacterial PGPRs, strain dependent effects on plant metabolome profile, bio-prospecting various isolates of bacterial PGPRs for potential secondary metabolites and non-target effects of PGPR on microbial community structure and functions. Section 3 encompasses synthesis of antimicrobial secondary metabolites from beneficial endophytes, bio-prospecting medicinal and aromatic hosts and effect of endophytic SMs on plants under biotic and biotic stress conditions. © Springer Nature Singapore Pte Ltd. 2019.
Towards a new horizon of sustainable agriculture with microorganisms useful in agriculture
(Elsevier B.V., 2021) Chetan Keswani; Ugo De Corato; Estibaliz Sansinenea; Sina M. Adl
[No abstract available]
Trichoderma spp. mediated induction of systemic defense response in brinjal against Sclerotinia sclerotiorum
(Elsevier Ltd, 2021) Satyendra Pratap Singh; Chetan Keswani; Surya Pratap Singh; Estibaliz Sansinenea; Trinh Xuan Hoat
Induction of resistance to pathogen is associated with the colonization of root by Trichoderma spp. has been attributed as one of the major mechanisms contributing to pathogenic invasion. The present study sheds light on the defense network of brinjal plant bioprimed with Trichoderma spp. challenged with Sclerotinia sclerotiorum. Plants treated with dual inoculation of Trichoderma harzianum and Trichoderma asperellum triggered further synthesis of TPC under S. sclerotiorum challenge with maximum increment recorded at 72 hours. In consortium treated and pathogen challenged plants, a higher amount of shikimic acid was observed at 72 hours, whereas other phenolics showed little differences among the treatments. The consortium treatment showed significantly higher defense related enzymes (Phenylalanine Ammonia Lyase, Peroxidase and Polyphenol Oxidase) activity than other treatments. The study signifies how Trichoderma spp. reprograms the host's defense network to provide robust protection against S. sclerotiorum. In the present case, overall protection was provided to the brinjal plants against the attack of S. sclerotiorum. © 2021 The Author(s)
Unraveling efficient strategies for inducing systemic resistance in crops for managing biotic stress
(Elsevier B.V., 2023) Chetan Keswani; Raffaella Balestrini; Tatiana Minkina; Satyendra Pratap Singh; Ugo De Corato; Estibaliz Sansinenea
[No abstract available]