Browsing by Author "Gaurav Kumar Pandey"

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Arbuscular mycorrhizal fungi as a potential biofertilizers for agricultural sustainability
(Open Science Publishers LLP Inc., 2022) Kumar Anand; Gaurav Kumar Pandey; Tanvir Kaur; Olivia Pericak; Collin Olson; Rajinikanth Mohan; Kriti Akansha; Ashok Yadav; Rubee Devi; Divjot Kour; Ashutosh Kumar Rai; Manish Kumar; Ajar Nath Yadav
Globally, by 2050, agricultural food production will be increased to feed the growing population. To achieve the objective in sustainable manner, scientific chronicles have explores the mutualistic interaction between plant roots and rhizosphere microbiome. One of the interactions of plants roots was found with arbuscular mycorrhiza fungi (AMF), a rhizosphere microbiome. Biofertilization process by the mean of AMF has depicted as a beneficial alternative to chemical fertilization practices. It has been recognized for having several potential applications such as plant fertilization (phosphorus, nitrogen and other micronutrients), alleviation of biotic (protecting plants from pest and pathogens), and abiotic stresses (drought, salinity, heavy metals, low and high temperature). AMF sustainably increases the plant growth and production by establishing within the host root with the help of set of genes and fulfilling the needs of the host. At present, worldwide total 340 species of AMF has been found. In the present review, global diversity, molecular crosstalk in AMF symbiosis and their potential application in sustainable agriculture has been reviewed. © 2022 Anand, et al.
Combination of EZH2 and ATM inhibition in BAP1-deficient mesothelioma
(Springer Nature, 2024) Nick Landman; Danielle Hulsman; Jitendra Badhai; Jawahar Kopparam; Julian Puppe; Gaurav Kumar Pandey; Maarten van Lohuizen
Background: More than half of mesothelioma tumours show alterations in the tumour suppressor gene BAP1. BAP1-deficient mesothelioma is shown to be sensitive to EZH2 inhibition in preclinical settings but only showed modest efficacy in clinical trial. Adding a second inhibitor could potentially elevate EZH2i treatment efficacy while preventing acquired resistance at the same time. Methods: A focused drug synergy screen consisting of 20 drugs was performed by combining EZH2 inhibition with a panel of anti-cancer compounds in mesothelioma cell lines. The compounds used are under preclinical investigation or already used in the clinic. The synergistic potential of the combinations was assessed by using the Bliss model. To validate our findings, in vivo xenograft experiments were performed. Results: Combining EZH2i with ATMi was found to have synergistic potential against BAP1-deficient mesothelioma in our drug screen, which was validated in clonogenicity assays. Tumour growth inhibition potential was significantly increased in BAP1-deficient xenografts. In addition, we observe lower ATM levels upon depletion of BAP1 and hypothesise that this might be mediated by E2F1. Conclusions: We demonstrated the efficacy of the combination of ATM and EZH2 inhibition against BAP1-deficient mesothelioma in preclinical models, indicating the potential of this combination as a novel treatment modality using BAP1 as a biomarker. © The Author(s) 2024.
CPW-fed fork shaped slotted antenna with dual-band notch characteristics
(IEEE Computer Society, 2014) Sachin Kalraiya; Hari Shankar Singh; Gaurav Kumar Pandey; Awadhesh Kumar Singh; Manoj Kumar Meshram
A coplanar waveguide (CPW) fed dual band-notched antenna for Ultra Wideband (UWB) and Bluetooth applications is presented. The proposed antenna consists of a planar fork shaped radiating patch and modified ground plane. The fork shaped radiating patch is fed with 50-0 CPW microstrip line. The proposed antenna has a total size of 25 × 30 mm2 and operates over the frequency band between 2.13 and 12.86 GHz. The conventional slots are created on the modified ground plane and radiating patch to obtained the band notched characteristics. The J-shaped slot is created on modified ground plane to obtained band notched at WiMAX (3.4 GHz-3.69 GHz) and U-shaped slot is created on feed line which is responsible for WLAN (5.15 GHz-5.85 GHz) band notched. The proposed antenna achieves good impedance matching, almost stable radiation patterns, and good peak realized gain over an operating band. © 2014 IEEE.
Genomic Imprinting: Insights into Diverse Epigenetic Regulatory Mechanisms
(S. Karger AG, 2025) Gaurav Kumar Pandey; Rajiva Raman
Background: Genomic imprinting is a well-known phenomenon in which certain genes are expressed in a sex-of-the-parent-specific manner, resulting in mono-allelic expression. Summary: Over the years, the diversity of mechanisms observed in imprinted gene clusters has provided a valuable model system for exploring the complexities of epigenetics, which can be extended to other cellular and disease models. This review examines these different mechanisms throughout early embryonic development and offers insights into the interactions among key players such as DNA methylation, histone modifications, and non-coding RNAs, as well as their regulatory impact on gene expression. Key Message: Genomic imprinting, although being a classical genetic concept, has emerged as a model system for understanding diverse epigenetic regulatory mechanisms. This review offers an overview of such regulatory mechanisms that have been learnt over the years through studies on imprinted clusters. © 2025 S. Karger AG, Basel.
Long Non-Coding RNAs: Tools for Understanding and Targeting Cancer Pathways
(MDPI, 2022) Gaurav Kumar Pandey; Chandrasekhar Kanduri
The regulatory nature of long non-coding RNAs (lncRNAs) has been well established in various processes of cellular growth, development, and differentiation. Therefore, it is vital to examine their contribution to cancer development. There are ample examples of lncRNAs whose cellular levels are significantly associated with clinical outcomes. However, whether these non-coding molecules can work as either key drivers or barriers to cancer development remains unknown. The current review aims to discuss some well-characterised lncRNAs in the process of oncogenesis and extrapolate the extent of their decisive contribution to tumour development. We ask if these lncRNAs can independently initiate neoplastic lesions or they always need the modulation of well characterized oncogenes or tumour suppressors to exert their functional properties. Finally, we discuss the emerging genetic approaches and appropriate animal and humanised models that can significantly contribute to the functional dissection of lncRNAs in cancer development and progression. © 2022 by the authors.