Browsing by Author "Gaurav Rathore"

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Derivation and characterization of a ES-Like cell line from Indian catfish heteropneustes fossilis blastulas
(2014) Anindya S. Barman; Kuldeep K. Lal; Gaurav Rathore; Vindhya Mohindra; Rajeev K. Singh; Akankaha Singh; Praveen Khare; Bechan Lal
A cell line designated as HFB-ES was established from blastula stage embryos of H. fossilis (Singhi). The embryonic cells were harvested and maintained in Leibovitz's medium supplemented with 15% fetal bovine serum. The cell line had been subcultured for more than 90 passages in a period of 24 months. HFB-ES cells were able to grow at temperatures between 25 and 35°C with an optimum temperature of 28°C. The growth rate of HFB-ES was proportional to FBS concentration, with optimum growth seen at 15% FBS concentration. The originality of the cell line was confirmed by sequencing of cytochrome oxidase c subunit I (COI), cytochrome b gene, and microsatellite DNA profile. Results of chromosome complements of HFB showed normal karyo-morphology with 56 (2 n) diploid number of chromosomes after 40 passages which indicated that the developed cell line is chromosomally stable. The pluripotency of HFB was demonstrated by alkaline phosphatase activity and Oct-4 gene expression. Expression of GFP reporter gene was successful in HFB-ES. These results indicated that HFB-ES could be utilized for future gene expression studies. © 2014 Anindya S. Barman et al.
Genotyping of Aeromonas hydrophila by Box elements
(2010) Vijai Singh; Dharmendra Kumar Chaudhary; Indra Mani; Pallavi Somvanshi; Gaurav Rathore; Neeraj Sood
PCR-based DNA fingerprinting techniques were evaluated to genotype eight diseased, particularly normal and environmental isolates of Aeromonas hydrophila. PCR-based fingerprinting method has an advantage of having repetitive sequence also called Box elements that are interspersed throughout the genome in diverse bacterial species. The BOX-PCR fingerprinting technique was evaluated for the discrimination of different isolates of A. hydrophila. All the studied isolates have shown major banding patterns ranged from 500-3000 bp. These finding could be advantageous to investigate the strain level specific fingerprints of A. hydrophila as potential genotypic markers. © 2010 Pleiades Publishing, Ltd.