Browsing by Author "Gurpreet Singh"

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Effect of 5-fluorouracil and mitomycin on the healing of intestinal anastomosis
(2007) Vijay K. Shukla; Manoj Pandey; P.C.L. Das; S.K. Tiwary; J.P.N. Chansouria; Mohan Kumar; Gurpreet Singh; S.K. Pandey; Gajendra Singh
Objective: Antineoplastic agents affect the healing of intestinal anastomosis. The aim was to evaluate the effect of 5-fluorouracil (5-FU) and mitomycin on the healing of the intestinal anastomosis and their schedule of administration. Material and Methods: Eighty-nine male albino Charles Foster rats with a mean weight of 256.57 g were divided into six groups. Group A represents the control, while in others varying schedules of chemotherapy (5-FU and mitomycin) were administered. The sacrifices were made on days 7, 14 and 21 postoperatively and bursting pressure and hydroxyproline content were measured. Results: Nine rats died before completion of the experiment and were excluded. Adhesions were noted in all rats on sacrifice. The mean bursting pressure of normal intestine (group A) was 252 mm Hg. The bursting pressure was lower on day 7 (208 mm Hg) and it subsequently increased by day 21 (230 mm Hg). The mean bursting pressure in groups B, C, D and E was 174, 194, 182 and 188 mm Hg and it subsequently increased to 232, 272, 244 and 286 mm Hg. There was no difference in the pattern of bursting pressure in colon and ileum. The mean hydroxyproline content of ileum (group A) on day 7 was 34.37 mg/g tissue. The hydroxyproline content of the ileum in groups B, C, D and E was 15.08, 27.03, 7.75 and 21.04 mg/g tissue. There was a significant decrease in hydroxyproline content following anastomosis and chemotherapy. Conclusions: The effect of chemotherapy was pronounced when administered on the day of surgery or in the immediate pre- or postoperative period. Hence administration of chemotherapy during this period may be harmful. Copyright © 2007 S. Karger AG.
Genetics of site specific colon cancer: a family study
(1984) R. Bamezai; Gurpreet Singh; N.N. Khanna; Shamer Singh
In a kindred of 46 individuals of an Indian Sikh family with no history of consanguinity, 8 suffered from site specific colon (caecum) cancer (not associated with polyposis). The clinical features in the affected kindred were indicative of a “cancer family syndrome”. The study of the family depicted an autosomal dominant transmission pattern of site specific colon cancer, with probably 100% penetrance in the affected individuals. A low percentage of mitotic index (MI) was observed in the two probands who were available for the study. No structural or numerical chromosomal aberrations were observed in either case. It is probable that the gene responsible in this family could have begun by mutation at the germ cell level in the 1st generation and remained in a heterozygous condition in the affected individuals. Copyright © 1984, Wiley Blackwell. All rights reserved
Immune status in advanced upper gastrointestinal cancers
(1985) Gurpreet Singh; S.N. Das; N.N. Khanna
Thirty‐eight cases of advanced upper gastrointestinal cancer were assessed for their immune status prior to any form of therapy. Cell‐mediated immunity, as tested by absolute lymphocyte count, T‐cell count and delayed cutaneous hypersensitivity reactions to purified protein derivative and phytohemagglutinin showed severe depression. Immunoglobulins A and M were elevated, while immunoglobulin G reduced. No correlation could be established between the immune status and the site of cancer or its extent, the performance status of the patients, or their response to chemotherapy. Copyright © 1985 Wiley‐Liss, Inc., A Wiley Company
Malformations of the eye resulting from maternal hypervitaminosis a during gestation in the rat
(1981) R. Padmanabhan; Gurpreet Singh; Shamer Singh
Vitamin A (1-1.5 lac IU) was administered orally to pregnant Wistar rats on days 8-14 of gestation (sperm + = day 0). Fetuses collected on day 21 revealed several anomalies including those of the eyes, e.g. open eyelids, exophthalmia, cataractous lens, microphthalmia and anophthalmia. Histological examination of treated eyes revealed degenerative changes in the cornea, lens and retina; shallowness or obliteration of anterior and vitreous chambers, and complete fusion of various structures with each other in some cases of microphthalmis. Anophthalmic cases showed no trace of any ocular structure. Retinae showed remarkable infoldings. The degenerative changes in retinae were associated with degeneration of optic nerve fibres. Eye malformations induced by hypervitaminosis A were stage dependent and mostly found in groups treated on day 8 and 9 of gestation. Reported cases of anomalies in humans associated with hypervitaminosis A during pregnancy warn us against the uncritical use of high doses of vitamin A during pregnancy. © 1981 S. Karger AG, Basel.
Nutritional status in advanced upper gastrointestinal cancer
(1985) Gurpreet Singh; N.N. Khanna
Fifty‐four patients with advanced upper gastrointestinal cancer were assessed for their nutritional status prior to any form of therapy. Anthropometric parameters—weight loss, arm muscle circumference, skin‐fold thickness—and biochemical parameters—serum albumin and delayed cutaneous hypersensitivity reactions to purified protein derivative (PPD) and phytohemagglutinin (PHA)—were used for nutritional assessment. Using standard values for comparison, about 80% of all patients were found to be nutritionally depleted. Immunocompetence did not show significant correlation with other nutritional parameters. Copyright © 1985 Wiley‐Liss, Inc., A Wiley Company