Browsing by Author "Guruprasad C. Nille"

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Characterization and potential novel applications of zinc-based traditional medicine, Yashad Bhasma
(Elsevier B.V., 2025) Guruprasad C. Nille; Monisha Bhuyan; Laxmi Narayan Gupta; Mohd Ali; Chandra Shekhar Pati Tripathi; Omkar S. Nille; Shardendu K. Mishra; Anuja A. Vibhute; Pranoti Anil Kamble; Himanshu Ranjan; Amaresh Kumar Singh; Arpita P-Tiwari; Anand Kumar Chaudhary
Background: Yashad Bhasma (YB), the incinerated metal ash of zinc, has been used for centuries in Ayurveda to address a variety of conditions, including eye diseases, diabetes mellitus, anemia, respiratory illnesses, etc. Objective: This research aimed to synthesize and characterize YB and to evaluate its potential antimicrobial, antioxidant, and anti-angiogenic activities. Materials and methods: In this study, YB is synthesized by optimizing the traditional method. Morphological and physicochemical characterization are performed using XRD, XPS, SEM, TEM, EDAX, DLS, TGA-DSC, and FTIR. The antimicrobial activity of YB is assessed using the well diffusion technique against the gram-positive bacterium Staphylococcus aureus (S. aureus) and the gram-negative bacterium Escherichia coli (E. coli). The antioxidant potential is evaluated using the 1,1-Diphenyl-2-Picrylhydrazyl (DPPH) radical scavenging assay and Nitric oxide (NO) radical scavenging assay. A chick chorioallantoic membrane (CAM) assay is performed on fertilized chick eggs to study the anti-angiogenesis potential of YB. Results: The XRD patterns of YB showed the presence of cubic and hexagonal phases of ZnS having average crystallite size of 32.66 nm. XPS data supports the formation of ZnS phase of YB. SEM and TEM data confirmed the size of YB NPs in a range of 250–350 nm. The EDAX analysis confirmed the presence of Zn (37.2 %) and S (21.18 %). The mean particle diameter was 361 nm in DLS. TGA-DSC findings verified that the synthesized material is stable up to 435.80 °C. The FTIR confirms the presence of organic moieties in YB along with ZnS phase. YB effectively inhibited the growth of S. aureus and E. coli. The ability of YB to scavenge DPPH and NO radicals is found to be concentration dependent (50–250 μg/mL). The study also demonstrated that YB has notable antioxidant activity. The disappearance of blood vessels beneath the sample-loaded disk after 7 days indicated the effective anti-angiogenic properties of YB. Conclusion: Altogether, YB exhibited significant antimicrobial, noteworthy antioxidant, and anti-angiogenic activities, indicating its potential as a promising therapeutic agent. © 2025 The Authors
Clinical evaluation of Talapotaka Churna – A polyherbal Ayurvedic formulation in type 2 Diabetes mellitus
(National Institute of Science Communication and Information Resources (NISCAIR), 2018) Guruprasad C. Nille; K.R.C. Reddy; J.S. Tripathi
To evaluate and compare the antidiabetic effect of Talapotaka Churna in Type II Diabetes mellitus patients were registered from S. S. Hospital, Banaras Hindu University, Varanasi. Patients were randomly divided in four groups for treatment of four different medicine groups: [Group A- Talapotaka Churna (4 g TID) with Takra (Buttermilk) before meal; Group B- Talapotaka Churna (4 g TID) with warm water before meal; Group C- Talapotaka Churna (4 g TID) with ongoing allopathic treatment with water before meal. Group D- Glimepride treated (1 mg BD) before meal]. The treatment was given for a period of two months. Fasting blood sugar, postprandial blood sugar, HbA1c, SGOT, SGPT, Sr. Creatinine and lipid profile level were estimated biochemically. The results were promising and reveal that Talapotaka Churna can be a safe, acceptable and effective alternative or adjuvant to the conventional oral hypoglycemic agents. Holistic principles of Ayurveda when used as supplementary for modern drugs in diabetes show their effects on all bodily symptoms and helps to minimize them in which modern drug fails. So, it is the need of time to support the modern medications with Ayurvedic therapy. © 2018, National Institute of Science Communication and Information Resources (NISCAIR). All rights reserved.
Comparative antidiabetic investigation of Talapotaka Churna and Avartaki Churna in STZ-induced diabetic rats
(BRNSS Publication Hub, 2016) Guruprasad C. Nille; Shardendu Mishra; Anshuman Trigunayat; K.R.C. Reddy
Aim: To compare the antidiabetic effect of Talapotaka Churna and Avartaki Churna in experimental animals. Materials and Methods: Talapotaka Churna (Avartaki [Cassia auriculata L.], Amalaki [Emblica officinalis G.], Haridra [Curcuma longa L.], and Daruharidra [Berberis aristata]) and Avartaki Churna (C. auriculata L.) were prepared by the standard procedure of Churna Kalpana. Diabetes was induced by streptozotocin (35 mg/kg) solution (intra-peritoneal). After assessment of hyperglycemia as an approximate induction of diabetes, a group of animals (TP300 and AV300) were treated with a dose of 300 mg/kg of Talapotaka Churna and Avartaki Churna each. For treatment comparison, Group III animals were treated with a standard antidiabetic drug, glibenclamide 1 mg/kg. Blood sugar and lipid profile level were estimated biochemically. Results: Talapotaka Churna and Avartaki Churna both reduced fasting blood glucose significantly on various doses in STZ-induced diabetic rats. Talapotaka Churna and Avartaki Churna also showed a reduction in the levels of total cholesterol, triglycerides, low-density lipoprotein-cholesterol, very low-density lipoprotein-cholesterol but it increases the levels of high-density lipoprotein-cholesterol in diabetic rats. Conclusion: Talapotaka Churna and Avartaki Churna have significant antidiabetic and antihyperlipidemic activities in Type 2DM rats, which seem to scientifically validate its traditional uses and might be promising drugs in the therapy of diabetes mellitus and its hyperlipidemic complications.
Frankincense unveiled: Phytochemistry, bioactivity, and future directions
(Elsevier B.V., 2025) Shardendu K. Mishra; Deepali Pandey; Omkar S. Nille; Shital J. Rajmane; Guruprasad C. Nille
Background: Frankincense, an aromatic resin, is obtained from a deep longitudinal slit in the trunk of the Boswellia plant (Family - Burseraceae). It is also known as olibanum. Amongst 30 different species of Boswellia, only four species, namely Boswellia serrata, Boswellia carteriii, Boswellia frereana, and Boswellia sacra, have been used for the commercial production of the olibanum. Purpose: This systematic review aims to offer a comprehensive overview of chemistry, phytochemistry, and biological activities of frankincense. This work provides essential information and various perspectives on frankincense for the purpose of discovering new drugs. Study Design: This review examines the chemical structures and biological activities associated with frankincense, utilizing several electronic databases, including PubMed, Web of Science, Google Scholar, and other research repositories. Methods: A systematic search technique was used to identify relevant databases that focus on the phytochemistry and biological activity of frankincense. A literature survey covering the last 25 years identified over 104 distinct phytochemicals from published databases. Results: Our exploration has covered various dimensions, including phytochemical analysis, biological effects, production methods, clinical applications, and identified research needs. Frankincense, derived from different Boswellia species, reveals a rich array of approximately 104 phytochemicals from various published sources. Numerous biological effects of frankincense have been reported, including anti-inflammatory, antioxidant, immunomodulatory, anticancer, and wound healing capacities. Conclusion: This systematic review covers the details of chemistry, phytochemistry, and biological activities of frankincense, and also warrants the need and scope of extensive future research. © 2025 The Author(s)
Hypoglycemic effect of Talapotaka Churna in streptozotocin-induced hyperglycemia in rats
(Medknow Publications, 2016) Guruprasad C. Nille; Shardendu Mishra; Anshuman Trigunayat; K.R.C. Reddy
Objective: To evaluate the antihyperglycemic effect of Talapotaka Churna in experimental animals, Talapotaka Churna was prepared, containing Avartaki (Cassia auriculata), Amalaki (Emblica officinalis), Haridra (Curcuma longa), and Daruharidra (Berberis aristata). Materials and Methods: Talapotaka Churna was prepared by the standard procedure of Churna Kalpana. Hyperglycemia was induced to create an equivalent to the diabetic state by giving streptozotocin (STZ) solution (intraperitoneal) 35 mg/kg. After assessment of hyperglycemia as an approximate induction of diabetes, group of animals (IV and V) were treated with doses 300 and 600 mg/kg of Talapotaka Churna. For treatment comparison, Group III animals were treated with a standard hypoglycemic drug, glibenclamide 10 mg/kg. Blood sugar, the level was estimated by glucometer on the 7th, 14th, 21st, and 28th day. Results: Talapotaka Churna produced a significantly lowering of fasting blood glucose with various doses in STZ-induced diabetic rats. In a 4-week study, Talapotaka Churna produced a significant reduction in blood glucose compared to glibenclamide. Conclusion: Talapotaka Churna and glibenclamide significantly lowered blood glucose level. The results were found more significant with respect to treatment days in this in vivo comparative study.
Potential implications of Ayurveda in Psoriasis: A clinical case study
(Elsevier B.V., 2021) Guruprasad C. Nille; Anand Kumar Chaudhary
Immune response of a human body to the uncertain factors leads to the accelerated inflammatory proliferation of the ailing cells of the skin known as Psoriasis. Although the condition found described many decades ago, the etiology and treatment look under-researched. In Ayurveda, many herbs have proven efficacy in psoriasis, but the multifaceted etiology of the disease needs a multimodal treatment approach. We report about Ayurveda treatment in a 68-year-old female patient with plaque psoriasis presented with erythematous plaques on the anterior surface of the legs, right forehand, and neck region. The Auspitz sign and Koebner phenomenon were positive. The treatment protocol was adopted as per Ayurvedic samprapti (pathophysiology) and the patient cured completely without reporting any adverse events after the one year of treatment. No recurrence observed even after one year of the halted treatment. The importance of a wholesome diet as a health promoter is also revalidated. Photographic documentation was recorded with the proper consent of the patient during successive treatment and regular follow-ups. Altogether, multimodal Ayurveda treatment led to speedy and substantial recovery from a chronic case of psoriasis. © 2020 The Authors
Safe and effective management of psoriasis through Ayurveda: A case report
(Elsevier B.V., 2025) Guruprasad C. Nille; Monisha Bhuyan; Laxmi Narayan Gupta; Anand Kumar Chaudhary
Ayurveda, an ancient system, offers in-depth insights into various skin disorders. It provides detailed understanding of causative factors and pathomechanisms, along with time-tested traditional treatments based on its principles. We report a 16-year chronic case of psoriasis (36-year-old male patient) with plaques and peeling erythrodermic skin rashes over the back and front of the trunk area and both legs. The scalp area and nail beds were also affected. The early involvement of joints was also noted. A two-year multimodal Ayurveda treatment has shown notable efficacy in managing chronic plaque and erythrodermic psoriasis, with no reported adverse events or side effects. The progressive events were documented in the form of photographs with the proper consent of the patient. The Ayurveda medicines, strict dietary regimen, and regular follow-ups together resulted in promising outcomes, revalidating the potential of Ayurveda in controlling psoriasis and its complications. © 2024 The Authors
Talapotaka Churna: A prudent permutation by Acharya Vallabhacharya
(Medknow Publications, 2016) Guruprasad C. Nille; Bhaswati Bhattacharya; Shital J. Rajmane; K.R.C. Reddy
Talapotaka Churna is one of the best classical formulations mentioned for the treatment of Prameha in Vaidya Chintamani. Each of the ingredients has been proven classically and scientifically to be very effective in the management of Prameha and diabetes mellitus, respectively. The view of Acharya Vallabhacharya toward the management of Prameha is straightforward by such a wise permutation in a specific proportion with precise Anupana. There are many such classical formulations where the logic behind combination by ancient Ayurveda scholars should be validated or compared with the corresponding modern entity. The same attempt has been made in this review, where it is observed that Acharya Vallabhacharya had prepared Talapotaka Churna with a scientific vision, as published research work supports the same.