Browsing by Author "Hari Om Singh"

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HRAMS Proteomics Insights on the Anti-Filarial Effect of Ocimum sanctum: Implications in Phytochemical-Based Drug-Targeting and Designing
(Multidisciplinary Digital Publishing Institute (MDPI), 2025) Ayushi Mishra; Vipin Kumar; Sunil Kumar; Hari Om Singh; Anchal Pratap Singh
Lymphatic filariasis (LF) continues to impact 657 million individuals worldwide, resulting in lifelong and chronic impairment. The prevalent anti-filarial medications—DEC, albendazole, and ivermectin—exhibit limited adulticidal efficacy. Despite ongoing LF eradication programs, novel therapeutic strategies are essential for effective control. This study examines the mechanism of action of Ocimum sanctum on the filarial parasites Setaria cervi via a synergistic biochemical and proteomics methodology. The ethanolic extract of Ocimum sanctum (EOS) demonstrated potential anti-filarial action in the MTT reduction experiment, with an LC50 value of 197.24 µg/mL. After EOS treatment, an elevation in lipid peroxidation (51.92%), protein carbonylation (48.99%), and NADPH oxidase (88.88%) activity, along with a reduction in glutathione (GSH) (−39.23%), glutathione reductase (GR) (−60.17%), and glutathione S transferase (GST) (−50.48%) activity, was observed. The 2D gel electrophoresis identified 20 decreased and 11 increased protein spots in the EOS-treated parasites relative to the control group. Additionally, in drug docking analysis, the EOS bioactive substances ursolic acid, rutin, and rosmarinic acid show a significant binding affinity with the principal differentially expressed proteins. This paper demonstrates, for the first time, that the anti-filarial efficacy of EOS is primarily facilitated by its impact on energy metabolism, antioxidant mechanisms, and stress response systems of the parasites. © 2024 by the authors.
IL-8 polymorphisms (−251T/A, −1633T/C) and their effects on COVID-19 clinical outcomes: An observational study
(Academic Press, 2025) Hari Om Singh; Josna Wilson; Goldi Namdev; Meenakshi Bhattacharya; Anchal Pratap Singh; Supriya D. Mahajan; Nemat Ali; Abdullah F. Alasmari
Background: COVID-19 presents with a wide spectrum of clinical outcomes, ranging from asymptomatic infection to critical illness. The cytokine storm is a hallmark of severe COVID-19 and is associated with elevated levels of pro-inflammatory cytokines, often observed in severe cases. Genetic polymorphisms in the IL-8 gene may influence individual responses to SARS-CoV-2 infection and the severity of the disease. Methods: A case-control study was conducted involving 200 COVID-19 patients and 201 healthy controls. Two IL-8 gene polymorphisms, −251T/A and +1633T/C, were genotyped using the PCR-RFLP (Polymerase chain reaction-restriction fragment length polymorphism) technique. Results: The IL-8 -251TA genotype and −251A allele were significantly associated with COVID-19 patients (P = 0.02, OR = 2.31; P = 0.03, OR = 1.38). The AT haplotype (−251A/+1633C) showed a non-significant trend toward increased risk for COVID-19 (P = 0.11, OR = 2.24). The IL-8 +1633 TT genotype was associated with impaired platelet counts among COVID-19 patients (P = 0.05, OR = 2.42). The IL-8 -251TA genotype was significantly associated with all stages of COVID-19 severity-critical (P = 0.05, OR = 8.15), severe (P = 0.002, OR = 2.74), moderate (P = 0.008, OR = 2.16), and mild (P = 0.01, OR = 2.89). In contrast, the IL-8 -251AA genotype was significantly associated with the critical stage (P = 0.04, OR = 2.53). A marginally significant association was observed between the IL-8 +1633CT genotype and the severe stage of COVID-19 (P = 0.07, OR = 2.13). Additionally, a borderline significant association was noted between elevated serum creatinine levels and the IL-8 -251AA genotype (P = 0.07, OR = 2.45). Conclusion: The IL-8 -251T/A polymorphism may serve as a potential risk factor for severe disease progression; the +1633 TT genotype may be linked to thrombocytopenia and poor clinical outcomes; and the −251AA genotype may be associated with elevated serum creatinine levels and an increased risk of renal dysfunction. © 2025 Elsevier Ltd