Browsing by Author "Harsh Pandey"

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Anti-oxidant, anti-apoptotic, anti-hypoxic and anti-inflammatory conditions induced by PTY-2 against STZ-induced stress in islets
(International Advancement Center for Medicine and Health Research Co., Ltd., 2019) Shivani Srivastava; Harsh Pandey; Surya Kumar Singh; Yamini Bhusan Tripathi
The earlier assessment of Pueraria tuberosa (PT) has shown anti-diabetic effects through enhancing incretin action and DPP-IV (Dipeptidyl peptidase-IV) inhibition. The aim of this work was to further explore the protective role of aqueous extract of Pueraria tuberosa tuber (PTY-2) against streptozotocin (STZ) induced islet stress in rats. Diabetes was induced by STZ (65 mg/kg body weight) in charles foster male rats. After 60 days of STZ administration, animals with blood glucose levels > 200 g/dL were considered as diabetic. All the rats were later divided into three groups: Group-1 (STZ untreated normal rats), Group-2 (Diabetic control), and Group-3 (PTY-2 [50 mg/100 g bw treatment for next 10 days to diabetic rats). The rats were then sacrificed after the 10th day of treatment accordingly. STZ treatment led to an increase in expression of Matrix metalloproteinases-9 (MMP-9), Tumour necrosis factor-α (Tnf-α), Hypoxia inducible factor-α (HIF-1α), Vascular endothelial growth factor (VEGF), Interleukin-6 (IL-6), Protein kinase C-ε (PKC-ε), Nuclear factor kappa-light-chainenhancer of activated B-cells (NFkB), and Caspase-3. Reverse Transcriptase-PCR (RT-PCR), Immunohistochemistry and Western-Blot analysis showed an increase in the expressions of Superoxide Dismutase (SOD) and Nephrin, and a decrease in the expressions of NFkB, PKC-ε, TNF-α, MMP-9, HIF-1α, VEGF, Caspase-3 and IL-6 after 10 days of PTY-2 treatment. The results showed that PTY-2 favorably changed all the expressions via anti-oxidant, antiapoptotic, anti-hypoxic and anti-inflammatory pathways, making itself as a protective agent against STZ induced islet stress. Further evaluation of PTY-2 might be helpful in establishing its role in the management of diabetes mellitus. © 2019, International Advancement Center for Medicine and Health Research Co., Ltd.
Assessment of Phytoremediation Potential of Algae for the Mitigation of Textile Effluents
(Springer Nature, 2025) Seema Nama; Vibha Jaiman; Anuj Sharma; Varad Nagar; Narain Bhoot; Harsh Pandey; Garima Awasthi
While the textile industry contributes significantly to the global economy, its environmental impact, which includes the discharge of pollutants such as dyes, heavy metals, and organic compounds, need long-term solutions. This study examines the phytoremediation capacities of three algal species, Chlorella, Euglena, and Oscillatoria, for which a macrophyte survey was conducted to reduce various water quality indicators in textile effluent in the drain region of Sanganeri printing companies from three different Bagru handblock print (S-1), Block print house (S-2), and Harion handblock printers. The study investigates how successful these algae are at extracting and detoxifying contaminants found in textile effluents. Pollutant absorption, algal growth features, and biochemical changes are monitored. The results show that Chlorella consistently lowered BOD and COD levels, but Euglena had inconsistent results. Oscillatoria exhibited significant variety. All three species showed decreases in overall hardness and alkalinity. All species lowered chloride, manganese, and iron concentrations, but Oscillatoria showed the greatest reduction. Nitrate reduction varied, but phosphate levels rose in all species. This work contributes to the promotion of phytoremediation as an effective tool for environmental restoration and aids in the development of low-cost, environmentally friendly alternatives to reduce the textile industry’s environmental impact. © The Author(s), under exclusive license to Springer Nature Switzerland AG 2025.
Corrigendum to “Incretin hormones receptor signaling plays the key role in antidiabetic potential of PTY-2 against STZ-induced pancreatitis” [Biomed. Pharmacother. 97 (330–338) 330–338] (Biomedicine & Pharmacotherapy (2018) 97 (330–338), (S0753332217332328) (10.1016/j.biopha.2017.10.071))
(Elsevier Masson SAS, 2018) Shivani Srivastava; Priya Shree; Harsh Pandey; Yamini Bhusan Tripathi
The authors regret the authors would like to change the word “Pancreatitis” to “β cells damage” throughout the manuscript, from heading to conclusion. The authors would like to change the third sentence of introduction “Thus, for diabetes progression, it is necessary to prevent the rate of pancreatitis.” to “Thus, for diabetes prevention, it is necessary to reduce the rate of β cells damage.” The authors would like to change the unit of glucose in Animal Design “200 g/dL” to 200 mg/dL.” The authors would like to change the name of secondary antibodies “anti-mouse-AF546 and anti-rabbit-AF488” to “anti-rabbit AF 546 (Red) and anti-mouse AF 488 (Green)” The authors would like to change the last sentence of discussion “All these changes collectively inhibit the apoptosis of pancreatic β cells through downregulation of Bcl 2 and improved insulin synthesis” to “All these changes collectively inhibit the apoptosis of pancreatic β cells through upregulation of Bcl 2 and improved insulin synthesis” The authors would like to apologise for any inconvenience caused. © 2017 Elsevier Masson SAS
Development and evaluation of herbal tablet loaded with Pueraria tuberosa water extract with use of different excipients
(BRNSS Publication Hub, 2018) Harsh Pandey; Shivani Srivastava; Brahmeshwer Mishra; Riden Saxena; Yamini Bhusan Tripathi
Aim: The aim of present paper is to develop the best quality of low cost tablets and with better effectiveness . Material and Methods: In this experiment we have used starch and some other excipients such as Microcrystalline cellulose, Acacia, Gellatin, Polyvinylpyrolidone and sodium Aliginate. The Water extract of plant Pueraria tuberosa belonging to the family of fabeacea. The herbal tablet of PT was made by wet granulation method. The finished products were characterized by standered method. For the determination of concentration, we have drawn the standard calibration curve of PT extract having absorption maxima at 221 nm in 0.1 N HCL solution. The drug obeyed Beer's lamberd law in the concentration range of 10 µg/ml to 50 µg/ml and was found to be linear with r2 =0.988 and regression equation y= 0. 003x- 0.009. Result and Discussion: The parameters of pre formulation such as Angle of repose, Carr's index and Husnner ratio showed good flow property. Tablets were evaluated by both official and non official testing, according to Indian Pharmacopoeia. After the official testing, the tablets made up by starch were not shown any significant change in properties . The friability of all formulations is less than 1%, except F0. Out of all, one of our formulation of herbal tablet mixed with 5% starch (FA) showed good release property within 1 hour in dissolution media, studied through in vitro method. Conclusion: These results concludes that starch can be proved as the better excipient for preparation of herbal tablets. © 2018 BRNSS Publication Hub. All rights reserved.
Expression kinetics reveal the self-adaptive role of β cells during the progression of diabetes
(Elsevier Masson SAS, 2018) Shivani Srivastava; Harsh Pandey; Yamini Bhusan Tripathi
Objective: To determine the histopathological and molecular changes in β-cells at different time intervals following streptozotocin (STZ)-induced diabetes. Methods: STZ (65 mg/kg body weight) was given to overnight fasted rats that were sacrificed after 1, 3, and 10 days of injection. Changes in islet morphology and in the expression of various factors involved in β-cell proliferation, inflammation and apoptosis were analyzed. Results: Superoxide dismutase (Sod) expression was completely reduced and that of NF-kB and iNOS were significantly increased, along with lymphocytic infiltration in the islets within 24 h of STZ injection. In addition, the β-cell protective markers Bcl-2, IL-6, Ki67, Hif-1α, VEGF and insulin were also enhanced, indicating a compensatory response of the β-cells to the initial damaging effects. Lymphocytic infiltration decreased after 3 days of injection, accompanied by enhanced expression of both GLP-1R and GIP R. The unresponsiveness of the incretin ligands after STZ administration further suggested a compensatory approach by the incretin receptors independent of glucose regulation. After 10 days, lymphocytic infiltration and inflammatory markers again increased, along with a concomitant reduction in the expression of incretin receptors, and upregulation of the protective markers. Furthermore, the saturation peak of blood glucose indicated progressive diabetes. Conclusions: The β-cells follow a biphasic pattern of expression of certain factors in order to achieve a balance between apoptosis, autophagy, neo-genesis, hypoxia and proliferation, and achieve homeostatic protection before the onset of diabetes. The drug interventions at an early stage, which are specific to these pathways, could be beneficial in preventing the progression of diabetes pathogenesis. © 2018 Elsevier Masson SAS
GLP 1 regulated intestinal cell’s insulin expression and self-adaptation before the onset of type 2 diabetes
(Tabriz University of Medical Sciences, 2019) Shivani Srivastava; Harsh Pandey; Surya Kumar Singh; Yamini Bhusan Tripathi
Purpose: Basically insulin is known to be secreted by β cells of the pancreas. Recently, it has also been found to be produced and expressed by intestinal epithelial cells with the help of L cells secreting glucagon like peptide 1 (GLP 1). Here, we have studied the same intestinal insulin expression property in T2D rats. Methods: Following 2 weeks of high fat diet (HFD) consumption, we have been given a single dose of streptozotocin (STZ) (35 mg/kg bw). Rats were then sacrificed after 1, 7 and 21 days. The GLP 1 analogue, liraglutide was also given to one group of diabetic rats, upto their respective durations. Intestinal cells apoptosis were checked by tunnel assay, Incretin hormones secretion and dipeptidyl peptidase 4 (DPP-IV) activity were analyzed through ELISA and immunohistochemistry was used to determine the insulin expression of intestine at different time interval during diabetes progression. Results: As compared to 1 and 21 days, we have found minor cells apoptosis in 7 days group along with high level of GLP 1 in diabetic model. Further, these effects were enhanced by liraglutide. In response to these we have found, decreased insulin expression after 21 days and with no significant effect upto 7 days in diabetic control groups. In contrast to this, GLP-1 level and insulin expression enhances prominently after 7 days of liraglutide treatment. Conclusion: These results explain the self-adapting approach of intestinal cells against diabetes onset and insulin expression enhancing property of liraglutide under stressful conditions. This study should be continued in future for the development of intestinal insulin producing drugs, to control diabetes under irreversible β cells damage. © 2019 The Author (s).
Hepatoprotective role of Pueraria tuberosa water extract (PTWE) in CCl4-induced liver injury through different signaling pathways
(Springer, 2024) Prerana Aditi; Vahab Ali; Mayank Choubey; Munichandra Babu Tirumalasetty; Harsh Pandey; Shivani Srivastava; Yamini Bhusan Tripathi
Liver damage is one of the leading diseases, resulting in high morbidity. It is more relevant in the context of bad food habits, environmental pollution, and biohazards. The present study aimed to investigate the role of semi-purified water extract of Pueraria tuberosa on carbon tetrachloride (CCl4)-induced liver injury and also its mechanism of action regarding transcriptomic status in liver tissue about inflammation, hypoxia, and apoptosis. Liver injury was induced in Charles foster rats via intraperitoneal injection (IP) of CCl4, 0.1 mg/100gm body weight, diluted with olive oil (30%) twice a week for 20 days. PTWE was given via oral route daily simultaneously with CCl4 at the dose of 50 mg/100 g and 100 mg/100 g body weight. On 21st day all rats were sacrificed. Biochemical tests and histological studies were done. mRNA expression of bcl-2, caspase-3, bax, and GAPDH and protein expression of iNOS, BCL-2, HIF-1α, VEGF, β-Tubulin was done. Simultaneous treatment of PTWE with CCl4 decreased the level of NO, PC, SGOT, SGPT, ALP, LPO, and iNOS, HIF-1α, VEGF, bax, and caspase-3 expression. In addition, PTWE increased the SOD, Catalase, GSH level, and bcl-2 expression as well as normalized the architecture of hepatic tissue. Immunohistochemical staining showed the decreased accumulation of CD45, VEGF, α-SMA, collagen, and desmin after PTWE treatment. This study suggests that PTWE inhibits fibrosis by reducing the accumulation of α-SMA, collagen, and desmin in CCl4-induced toxicity. The mechanism of protective action is through its anti-inflammatory (iNOS, NO, CD45), anti-apoptotic (bcl-2, bax, caspase-3), anti-hypoxic (HIF-1α, VEGF), and anti-fibrotic (α-SMA, collagen, desmin) potentials. © The Author(s), under exclusive licence to Institute of Korean Medicine, Kyung Hee University 2024.
Hepatoprotective role of Pueraria tuberosa water extract (PTWE) in CCl4-induced liver injury through different signaling pathways
(Springer, 2025) Prerana Aditi; Vahab Ali; Mayank Choubey; Munichandra Babu Tirumalasetty; Harsh Pandey; Shivani Srivastava; Yamini Bhusan Tripathi
Liver damage is one of the leading diseases, resulting in high morbidity. It is more relevant in the context of bad food habits, environmental pollution, and biohazards. The present study aimed to investigate the role of semi-purified water extract of Pueraria tuberosa on carbon tetrachloride (CCl4)-induced liver injury and also its mechanism of action regarding transcriptomic status in liver tissue about inflammation, hypoxia, and apoptosis. Liver injury was induced in Charles foster rats via intraperitoneal injection (IP) of CCl4, 0.1 mg/100gm body weight, diluted with olive oil (30%) twice a week for 20 days. PTWE was given via oral route daily simultaneously with CCl4 at the dose of 50 mg/100 g and 100 mg/100 g body weight. On 21st day all rats were sacrificed. Biochemical tests and histological studies were done. mRNA expression of bcl-2, caspase-3, bax, and GAPDH and protein expression of iNOS, BCL-2, HIF-1α, VEGF, β-Tubulin was done. Simultaneous treatment of PTWE with CCl4 decreased the level of NO, PC, SGOT, SGPT, ALP, LPO, and iNOS, HIF-1α, VEGF, bax, and caspase-3 expression. In addition, PTWE increased the SOD, Catalase, GSH level, and bcl-2 expression as well as normalized the architecture of hepatic tissue. Immunohistochemical staining showed the decreased accumulation of CD45, VEGF, α-SMA, collagen, and desmin after PTWE treatment. This study suggests that PTWE inhibits fibrosis by reducing the accumulation of α-SMA, collagen, and desmin in CCl4-induced toxicity. The mechanism of protective action is through its anti-inflammatory (iNOS, NO, CD45), anti-apoptotic (bcl-2, bax, caspase-3), anti-hypoxic (HIF-1α, VEGF), and anti-fibrotic (α-SMA, collagen, desmin) potentials. © The Author(s), under exclusive licence to Institute of Korean Medicine, Kyung Hee University 2024.
Histopathological Study Of Different Organs Of Charles Foster Rats Under The Exposure Of Pueraria Tuberosa
(West Bengal Veterinary Alumni Association, 2021) Harsh Pandey; Shivani Srivastava; Mohan Kumar; Yamini BhusanTripathi
The present study was undertaken to investigate the safe doses of Pueraria tuberosa water extract (PTWE) on different organs. The OECD guidelines 407 of repeated toxicity was followed with respect to the selection of dose and days for different organs. The selected doses of PTWE were 250, 500, 1000 and 2000 mg/kg b wt for 7, 14, 21 and 28 days. Haematoxylin and eosin staining was used to study the morphological alterations in heart, intestine, testis, adrenal gland and spleen. In the present study, no adverse alterations in cardiac fibers of the heart, size and shapes in crypts and villi of intestine, seminiferous tubules and spermatozoa count in testis, three zones of adrenal gland, and spleen were seen in all treated groups of PTWE. There were no adverse morphological alterations found in described organs. The PTWE are safe at 1000 mg/kg b wt. up to 28 days and 2000 mg/ kg b. wt up to 21 days, respectively © 2021, Exploratory Animal and Medical Research. All Rights Reserved.
In silico screening of Pueraria tuberosa (PTY-2) for targeting COVID-19 by countering dual targets Mpro and TMPRSS2
(Taylor and Francis Ltd., 2022) Priya Shree; Priyanka Mishra; Prateek Kumar; Harsh Pandey; Rajanish Giri; Radha Chaube; Neha Garg; Yamini Bhusan Tripathi
COVID-19 pandemic was started in Wuhan city of China in December 2019; immensely affected global population. Herein, an effort was made to identify potential inhibitors from active phytochemicals of Pueraria tuberosa (PTY-2) via molecular docking study. Our study showed five potential inhibitors (Robinin, Genistin, Daidzin, Hydroxytuberosone, Tuberostan) against Mpro and five inhibitors (Robinin, Anhydrotuberosin, Daidzin, Hydroxytuberosone, Stigmasterol) against TMPRSS2. Out of these, Robinin, Daidzin and Hydroxytuberosone were common inhibitors for Mpro and TMPRSS2. Among these, Robinin showed the highest binding affinity, therefore, tested for MD simulation runs and found stable. ADMET analysis revealed the best-docked compounds are safe and follow the Lipinski Rule of Five. Thus, it could be suggested that phytochemicals of PTY-2 could serve as potential inhibitors for COVID-19 targets. Communicated by Ramaswamy H. Sarma. © 2021 Informa UK Limited, trading as Taylor & Francis Group.
Incretin hormones receptor signaling plays the key role in antidiabetic potential of PTY-2 against STZ-induced pancreatitis
(Elsevier Masson SAS, 2018) Shivani Srivastava; Priya Shree; Harsh Pandey; Yamini Bhusan Tripathi
Aims Incretin therapy is one of the most potential approaches in the treatment of diabetes. In contrast to markedly available drugs, the herbal incretin modulators have lesser side effects with low economic cost. The main aim of this work was to analyze the potential of previously reported DPPIV inhibitor, aqueous extract of Pueraria tuberosa tubers (PTY-2) as incretin hormones receptor agonist against streptozotocin (STZ)-induced diabetes. Methods Chronic diabetes was induced with STZ (65 mg/kg bw) in rats for 60 days and grouped into diabetic control and PTY-2. Expression of genes was assessed by PCR, IHC, and ELISA. Morphological analysis of tissue was observed using H & E stain. In silico molecular docking approach has been used to see the interaction of active phytochemicals of PTY-2 on the basis of their binding energy [kcal/mol] and dissociation constant [pM] using YASARA software. Interactive visualization was done using Discovery studio 3.0. Results In comparison to diabetic control, the size and number of islet cells along with the plasma level of GLP-1, GIP, and pancreatic expressions of GLP-1R, GIP-R, Bcl2, and insulin were enhanced significantly after PTY-2 treatment. Through in silico molecular docking, tuberostan showed the best interaction for GLP-1R with binding energy at 8.15 kcal/mol and dissociation constant at 1061624.125 pM. Puererone showed the best interaction for GIP-R with binding energy at 8.31 kcal/mol and dissociation constant at 810381 pM. Conclusions In addition to previously studied DPPIV inhibitor, PTY-2 also acts as incretin receptors agonist and protects against STZ-induced diabetes by down regulating β cells apoptosis. © 2017 Elsevier Masson SAS
Pharmacokinetics Study Of Puerarin Absorption In Blood After Consumption Of Pueraria Tuberosa Water Extract (PTWE) By Rats
(West Bengal Veterinary Alumni Association, 2019) Harsh Pandey; Shivani Srivastava; Ashutosh Kumar; Rajesh Kumar; Yamini Bhusan Tripathi
The aim of the study was to analyse pharmacokinetics of puerarin absorption from blood Pueraria tuberosa tuber water extract (PTWE) and its hypoglycaemic response. Peaks of puerarin have been standardized with different types of mobile phase at 254 nm by HPLC-UV and prepared the calibration curve of puerarin. The extract was administered to the rat and their blood was collected at different time interval. The circulating PTWE in plasma was extracted with the help of acetonitryl by liquid-liquid extraction method. The extracted samples were then injected into the HPLC in order to estimate the puerarin absorption from PTWE in blood at different time interval. The concentration of blood puerarin was calculated by plotting its standard calibration curve. Further hypoglycaemic response of PTWE was also studied in diabetic rat model. The absorbed puerarin peak was found at 254 nm and its retention time (RT) is 1.6 minutes by using the mobile phase of Methanol: Ammonium acetate 10 mM buffer (60:40). After calibration, the value of Cmaxwas 21.04 μg/ml, Tmax4 hr and total AUC as 209.04 μg hr/ml of absorbed puerarin. Through in vivo study, it has also been found that PTWE significantly reduces the enhanced hyperglycaemia in diabetic rats, within 8 days of treatment. The present study clearly indicated the puerarin concentration was maximum at a time interval of 4 hr and exist in rat blood plasma upto 12 hours after oral consumption of PTWE. PTWE down regulates hyperglycemia, thus act as potential anti-diabetic drug responding in short duration of time. In addition, these results provide a new approach to study the pharmacokinetic evaluation of herbal drugs (PTWE) by estimating active components (puerarin) from herbal drugs. © 2019. All Rights Reserved.
Pueraria tuberosa as dipeptidyl-peptidase-iv inhibitor prevents streptozotocin-induced intestinal stress
(Open Access Macedonian Journal of Medical Sciences, 2021) Shivani Srivastava; Harsh Pandey; Surya Kumar Singh; Yamini Bhusan Tripathi
BACKGROUND: Enhanced expression of dipeptidyl-peptidase-IV (DPP-IV) has been found in various intestinal diseases. Pueraria tuberosa tuber water extract (PTY-2) is already known to have DPP-IV inhibitory potential. At the mRNA level, this inhibition has not yet been investigated. Increased incretin secretion due to inhibition of DPP-IV could lead to the suppression of stress and apoptosis of intestinal cells. AIM: In this research, we tried to study the antioxidant, anti-apoptotic, and DPP-IV inhibitory effect of PTY 2 against intestinal damage induced by STZ. METHODS: We found morphological damage to the intestine following streptozotocin (STZ) injection (65 mg/kg bw) in male Charles foster rats through histological examination. Superoxide dismutase (SOD) and DPP-IV mRNA expressions were analyzed by polymerase chain reaction and cell apoptosis was examined by tunnel assay and Bcl 2 immunoexpression. RESULTS: In STZ-induced diabetic control, the number and length of villi were decreased, but these damages were reversed by 10 days of PTY-2 treatment. SOD expression was found to be decreased whereas DPP-IV expression was enhanced with significant intestinal cell apoptosis in the diabetic control group. Treatment with PTY-2 decreases stress by upregulating SOD expression and by downregulating the expression of DPP-IV. These PTY-2 recoveries contribute to the suppression of apoptosis in the intestinal cells. CONCLUSION: The protective action of PTY-2 against STZ mediated intestinal damage is demonstrated by these short studies. Therefore, PTY-2 may be taken as a herbal remedy for diabetes-induced intestinal damages. © 2021 Shivani Srivastava, Harsh Pandey, Surya Kumar Singh, Yamini Bhusan Tripathi.
Toxicity profile of honey and ghee, when taken together in equal ratio
(Elsevier Inc., 2020) Prerana Aditi; Shivani Srivastava; Harsh Pandey; Yamini Bhusan Tripathi
Honey and ghee are an essential component of our diet. They play an important role like anti-inflammatory, antioxidative, antimicrobial, etc. It is written in Charak Samhita that an equal mixture of honey and ghee turn into a harmful component for health. This study was designed to explore the mechanism of toxicity through the biochemical and histological parameters in Charles foster rats (24 rats were used). We have divided these rats into four groups (n = 6) - normal, honey (0.7 ml/100 g bw), ghee (0.7 ml/100 g bw), and honey + ghee (1:1) (1.5 ml/100 g bw). Treatment was given orally for 60 days. All rats were sacrificed on 61 days. Biochemical parameters like liver function test, kidney function test, Oxidative stress, Glycemic, and some protein modification parameters were done in blood plasma. We found weight loss, hair loss, red patches on ear, and increased liver function test, oxidative stress, Amadori product formation, advanced glycation end-product formation, dipeptidyl protease (DPP-4) and decreased incretins (glucagon-like peptide-1(GLP-1) and gastric inhibitory polypeptide (GIP)) in honey + ghee group. H&E and immunohistochemistry results showed mild inflammation in liver tissue but no changes in the kidney, intestine and, pancreas. Thus it concluded that the increased formation of Amadori product, DPP-4 activity and low incretins (GLP-1, GIP) activity resulting high postprandial hyperglycemic response could be collectively responsible for oxidative stress-mediated toxicity of honey and ghee in the equal mixture. © 2020 The Authors